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The effect of missing feed intake records on the estimate of feed intake in cattle over a 70-day test period was studied. Two datasets, containing several cattle breeds at different ages, were analysed. The first dataset comprised 300-day-old Angus cattle, while the second dataset consisted of 900-day-old Limousin and Jersey crossbred cattle. A first-, second- and third-order polynomial and a non-linear function were fitted to the intake data. The 3 polynomial functions showed minimal differences in their goodness of fit to the data, with the non-linear function explaining the least variation in intake. Both datasets were analysed over a 70-day test period and shortened tests were compared to the full 70-day test. Tests were shortened by randomly deleting intake records or periodically deleting data on a daily or weekly basis. The effect of missing data on feed intake estimates was evaluated using Pearsons correlation and t-tests. The results suggest that a greater proportion of data can be removed randomly and daily, compared with missing data in weekly blocks. However, when data are removed in weekly blocks, removal in the middle of the test period gives a more accurate estimate of feed intake. It appears that in young growing animals, estimation of daily feed intake is more sensitive to missing feed intake records than for older cattle.

Animal GeneticsVolume 35, Issue 2 p. 163-163 Physical mapping of the stearoyl-CoA desaturase (SCD) locus in sheep H. Kuchel, H. Kuchel Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this authorB. D. Siebert, B. D. Siebert Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this authorC. D. K. Bottema, C. D. K. Bottema Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this authorG. C. Webb, G. C. Webb Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this authorA. M. Crawford, A. M. Crawford AgResearch, Molecular Biology Unit, Biochemistry Department, University of Otago, Box 56, Dunedin, New ZealandSearch for more papers by this authorS. J. Duncan, S. J. Duncan AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New ZealandSearch for more papers by this authorP. A. McDonald, P. A. McDonald AgResearch, Molecular Biology Unit, Biochemistry Department, University of Otago, Box 56, Dunedin, New ZealandSearch for more papers by this authorJ. C. McEwan, J. C. McEwan AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New ZealandSearch for more papers by this authorW. S. Pitchford, W. S. Pitchford Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this author H. Kuchel, H. Kuchel Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this authorB. D. Siebert, B. D. Siebert Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this authorC. D. K. Bottema, C. D. K. Bottema Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this authorG. C. Webb, G. C. Webb Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this authorA. M. Crawford, A. M. Crawford AgResearch, Molecular Biology Unit, Biochemistry Department, University of Otago, Box 56, Dunedin, New ZealandSearch for more papers by this authorS. J. Duncan, S. J. Duncan AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New ZealandSearch for more papers by this authorP. A. McDonald, P. A. McDonald AgResearch, Molecular Biology Unit, Biochemistry Department, University of Otago, Box 56, Dunedin, New ZealandSearch for more papers by this authorJ. C. McEwan, J. C. McEwan AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, New ZealandSearch for more papers by this authorW. S. Pitchford, W. S. Pitchford Animal Science, University of Adelaide, Roseworthy Campus, Roseworthy, SA 5371, AustraliaSearch for more papers by this author First published: 17 March 2004 https://doi.org/10.1111/j.1365-2052.2004.01114.xCitations: 4 W. S. Pitchford ([email protected]) Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat No abstract is available for this article.Citing Literature Volume35, Issue2April 2004Pages 163-163 RelatedInformation

SUMMARY The aim of this study was to investigate the relationships between various estimates of marbling in Jersey and Limousin crossbred steers with intramuscular fat percentage (IMF%) as determined by solvent extraction. The correlations between IMF% and various marbling scores ranged from 0.67 to 0.79. AUS-MEAT, MSA or USDA scoring systems were correlated, and ranked animals similarly. However, the best correlation with IMF% was found to be with the AUS-MEAT marbling score. When the range of IMF% was assigned to different scores within each system, the AUS-MEAT and USDA systems were similar whereas MSA scores had a higher concentration of fat per score and a broader range of concentrations. The only factors influencing assessment of marbling were IMF% and breed. Other traits, such as loin temperature, eye muscle area, melting point, fat colour and meat colour were not significant. Jersey cross steers had higher marbling scores (1.9 vs 0.8) and IMF% (6.4 vs 4.4%) than Limousin cross. A breed by sire interaction in marbling was also detected.

The importance of direct genetic, maternal, heterosis and epistatic effects were examined on post-weaning weight, height, length, girth, fat depth and muscle (ratio of stifle to hip width) with dry and wet season gains in these traits. The breeds used were two pure breeds (Jersey and Limousin), the LimousinxJersey F-1, and two backcrosses (F(1)xJersey dams and F(1)xLimousin dams). Direct genetic effects were large (p<0.001) for all traits except for length. Jersey maternal effects were large for weight, girth, fat depth and muscle in the post-weaning wet season gains which is an evidence of the impact of Jersey dam on progeny beyond weaning. There were large heterosis effects on fat depth and muscle relative to other traits. Epistatic effects were observed for post-weaning performance in weight, girth, fat depth and muscle. There are indications that there were different genetic effects for post-weaning compared to pre-weaning growth traits. Thus, it could be hypothesized from this study that different quantitative trait loci (QTL) affect early and late growth in Jersey and Limousin cross cattle breeds. The follow up work will examine the different chromosomal gene effects on pre- and post-weaning growth.

This study describes development of a consensus genetic linkage map of bovine chromosome 24 (BTA24). Eight participating laboratories contributed data for 58 unique markers including a total of 25 409 meioses. Eighteen markers, which were typed in more than one reference population, were used as potential anchors to generate a consensus framework map. The framework map contained 16 loci ordered with odds greater than 1000:1 and spanned 79.3 cM. Remaining markers were included in a comprehensive map relative to these anchors. The resulting BTA24 comprehensive map was 98.3 cM in length. Average marker intervals were 6.1 and 2.5 cM for framework and comprehensive maps, respectively. Marker order was generally consistent with previously reported BTA24 linkage maps. Only one discrepancy was found when comparing the comprehensive map with the published USDA-MARC linkage map. Integration of genetic information from different maps provides a high-resolution BTA24 linkage map.

During a 5-yr period, 1994-1998, pre-weaning and weaning data were collected on 591 calves produced by mating either straightbred Jersey, straightbred Limousin or F-1 (Limousin x Jersey) bulls to mature purebred Jersey or Limousin cows. Traits recorded included birth and weaning weight, height, length, girth, fat depth and a measure of muscle (ratio of stifle to hip width expressed as a percentage). All traits were analyzed assuming a model with sire and dam random effects that included effects of year and date of birth, sex, breed and yearxsex interaction. Main effects were generally significant with few exceptions. Direct genetic effects were large for weight, height, girth and muscle with a breed trend from purebred Jersey (small) to purebred Limousin (large). At weaning, the maternal effect of the Jersey dam was positive for weight (10.9+/-4.9 kg), girth (3.7+/-1.0 cm) and muscle (6.0+/-0.9%). Heterosis was highly significant and positive only for fat depth (1.5+/-0.2 mm) with the F-1 progeny being the fattest, followed by the backcrosses, then purebred Jersey and purebred Limousin. Also, significant (p<0.001) but negative heterosis was observed for weight, girth and muscle. The change in ranking for fat depth relative to other traits is a reflection of the large heterotic effects relative to direct effects on fat depth. Epistatic effects were not significant on any trait at birth or weaning. This study has indicated the possibility of exploiting the positive heterotic and maternal effects for fat depth and muscularity to meet specific meat quality and quantity demand by consumers.

Mature Hereford cows (766) were mated to 97 sires from seven breeds (Jersey, Wagyu, Angus, Hereford, South Devon, Limousin, and Belgian Blue), resulting in 1,215 calves born over 4 yr (1994 to 1997). These cattle comprised Australia's 'Southern Crossbreeding Project." Heifers were slaughtered at an average of 16 mo with hot standard carcass weight of 219 kg and 9 mm fat over the rump. Steers were slaughtered at an average of 23 mo with carcass weight of 319 kg and 13 mm fat over the rump. Meat and fat samples were taken from the carcass on the day after slaughter for subsequent laboratory analysis of i.m. fat content and fatty acid composition. Data were analyzed using uni- and bivariate animal models containing fixed effects of cohort, management group, birth month, and sire breed. March-born calves had fat with a 0.5 degrees C lower melting point, 0.6% higher total monounsaturated fatty acids, and 0.7% higher fatty acid desaturation index than calves born in April. Steers born in 1997 were the only cohort finished on pasture, and they had much more yellow fat than the other cohorts. Four heavy breed crosses (Angus, South Devon, Limousin, and Belgian Blue) averaged 284 kg carcass weight, followed by purebred Hereford (268 kg), Wagyu (244 kg) and Jersey (236 kg). Angus had the greatest fat depth (14.3 mm), ahead of Hereford and Wagyu (11.9 mm), Jersey (10.7 mm), South Devon and Limousin (9.9 mm) and Belgian Blue (8.0 mm). Jersey, Wagyu, and Angus had themost i.m. fat (4.6%), followed by Hereford and South Devon (3.8%), and Limousin and Belgian Blue (3.1%). The highly marbled Jersey and Wagyu had softer fat (6% lower fat melting point) than the other breeds. Angus were more highly marbled, similar to Jersey and Wagyu, but had harder fat similar to the leaner breeds. Heritabilities for all traits were low to moderate (16 to 36%). Genetic correlations between fatty add composition and carcass traits were not significant, indicating little evidence of antagonisms between traits that would prevent genetic progress in both production and quality.

The results of genotypic data contributed to the International Society for Animal Genetics (ISAG) Bovine Chromosome 27 Workshop are presented. Eight laboratories contributed 23 261 informative meioses from 44 loci. Eighteen loci were typed by at least two laboratories and were used to construct a consensus linkage map. Twenty-one loci were subsequently incorporated into a comprehensive map. The sex-averaged consensus map covered 66.9 cM. The sex-averaged comprehensive map was 75.5 cM, while the female and male maps were 73.1 and 63.7 cM, respectively. Five loci were excluded from the analysis because of ambiguous position in the linkage group and a low LOD score (less than 2.0). Average distance between loci in the comprehensive map was 1.98 cM.

Mammals possess multiple, closely linked beta-globin genes that differ in the timing of their expression during development. These genes have been thought to be derived from a single ancestral gene, by duplication events that occurred after the separation of the mammals and birds. We report the isolation and characterization of an atypical beta-like globin gene (omega-globin) in marsupials that appears to be more closely related to avian beta-globin genes than to other mammalian beta-globin genes, including those previously identified in marsupials. Phylogenetic analyses indicate that omega-globin evolved from an ancient gene duplication event that occurred before the divergence of mammals and birds. Furthermore, we show that omega-globin is unlinked to the previously characterized beta-globin gene cluster of marsupials, making this the first report of an orphaned beta-like globin gene expressed in a vertebrate.

The results of genotypic data contributed to the International Society of Animal Genetics (ISAG) Bovine Chromosome 11 (BTA11) Workshop are presented. Six laboratories contributed a total of 26 199 informative meioses from 80 loci. Thirty-six loci were typed by at least two independent laboratories and were used to construct a consensus linkage map of the chromosome. The remaining loci were subsequently incorporated into a comprehensive map. The sex-averaged consensus map covered 128.9 cM. The female consensus map was 101.2 cM, while the male consensus map was 129.8 cM. The comprehensive sex-averaged map was 134.2 cM and the average genetic distance between loci was 1.72 cM.

The Eph family of receptor tyrosine kinases plays a crucial role during development and is implicated in oncogenesis. Using a partial cDNA clone of an Eph-related kinase (Esk) we isolated the complete coding region of a gene which we show to be murine EphA1 by both structural and functional criteria. The chromosomal localization is shown to be syntenic to hEphA1 and the genomic organization also shows distinct features found in the hEphA1 gene. Functionally, in keeping with findings for the human homologue, both soluble recombinant and "native" mEphA1 show preferential binding to ephrin A1. However, we also observed significant binding to other A-type ligands as has been observed for other Eph receptors. We analysed the expression of mEphA1 mRNA by in situ hybridization on tissue sections. mEphA1 was expressed in epithelial elements of skin, adult thymus, kidney and adrenal cortex. Taken together with previous Northern blotting data these results suggest that mEphA1 is expressed widely in differentiated epithelial cells.

SummaryAdipose tissues from the 12th–13th rib interface were sampled at weaning (324 cattle) and slaughter (310 cattle). The animals were progeny from Hereford dams sired by Angus, Belgian Blue, Hereford, Jersey, Limousin, South Devon and Wagyu. Fatty acid composition of the triacylglycerol fraction at both stages was measured by gas‐liquid chromatography. Estimates of heritability and genetic and phenotypic correlations at weaning and slaughter were computed by restricted maximum likelihood using a sire model in both univariate and multivariate analyses. Results indicated that generally, there were strong, positive genetic correlations between fatty acids at weaning and slaughter (as high as 0.98). Phenotypic correlations were however, low and poor (0.04–0.44). Heritability (h2) estimates at weaning were low to moderate, ranging from 0–0.31. However, at slaughter, h2 estimates were generally higher than at weaning: Stearate, oleate and total monounsaturates had h2 estimates of 0.43, 0.37 and 0.40, respectively. Other carcass traits such as marbling score and melting point of fat had h2 estimates of 0.20 and 0.52, respectively. Significant breed, sex and location differences in fatty acid composition were also observed at weaning and slaughter.

Summary Subcutaneous adipose tissues were biopsied in purebred Jersey (n=17), purebred Limousin (n=17) and reciprocal F 1 Jersey × Limousin crossbred (n=33) calves at the age of 9–10 months. Triacylglycerol fatty acids were extracted and analysed for sex and breed differences. Heterosis, additive and maternal variances were estimated. All calves were pasture‐fed in a single management group and biopsied from the same anatomical site. Heifer calves had significantly higher proportions of palmitoleate, total mono‐unsaturated fatty acids, desaturation index and lower stearate than steer calves. Significant breed differences were observed in that Limousin calves had the highest proportions of palmitate and total saturated fatty acids, whereas Jersey calves had the most palmitoleate and desaturation index. Dominance effects were evident in the proportions of palmitate, stearate, desaturation and elongation enzyme indices due to the observed highly significant heterosis effect. Myristate, palmitate and total saturated fatty acids were considered heritable due to the observed highly significant additive genetic effect.

SummaryFatty acid composition, marbling score and melting point data collected between 1994 and 1996 were analysed. The data were from the adipose tissue of 764 Angus, Belgian Blue, Hereford, Jersey, Limousin, South Devon and Wagyu crossbred cattle slaughtered after lot‐feeding at 500 days of age. The aim was to investigate sire‐breed differences and to estimate heritability and genetic and phenotypic correlations. Significant breed differences were found: Jersey crosses had the highest marbling score and Belgian Blue crosses had the lowest. Limousin crosses had the highest melting point and Jersey crosses the lowest. South Devon crosses had the highest proportion of stearate and Jersey crosses the lowest. Desaturation indices in C16 and C18 fatty acids were highest in Jersey crosses and lowest in Limousin and South Devon crosses. In contrast, there were no breed differences in the proportions of palmitate, oleate, total saturated, total mono‐unsaturated fatty acids and elongation index. Heritability estimates of individual fatty acids and their summations, melting point and marbling were low to moderately low (0.05–0.27). Strong genetic correlations of melting point and desaturation index in C16 fatty acids (‐0.93), melting point and stearate (0.62), marbling and stearate (‐0.71) and marbling and desaturation index in C18 fatty acids (0.62) were observed. Phenotypic correlation were generally low. The results imply that fatty acids in the adipose tissue of lot‐fed cattle have a moderately low heritability, hence genetic progress might be slow.

SummaryLongissimus dorsi muscle tissue was biopsied between the 12th and 13th ribs of 96 purebred Jersey, purebred Limousin and Jersey × Limousin crossbred calves at the age of 9–10 months. Fatty acids of the phospholipid fraction were extracted and analysed for sex and breed differences. Heterosis, additive and maternal variances were estimated. All calves grazed pasture in a single management group and were biopsied from the same anatomical site. Steer calves had significantly higher proportions of the 14 : 0, 14 : 1, 18 : 2, 20 : 3 fatty acids and less of the 16‐di‐methyl‐acetal than heifer calves. Significant breed differences were observed: Limousin calves had the highest proportions of 16 : 0, 24 : 0 and saturated fatty acids (SFA), whereas Jersey×Limousin calves had the most 18 : 0 and elongation index. Dominant effects were evident in the proportions of 16 : 0, 18 : 0, SFA and elongation index. Additive genetic effects were significant in the proportions of 16 : 0, 18 : 0, 18 : 1n‐9, total SFA and desaturation and elongation indices. Combined maternal and additive effects were significant for long chain polyunsaturated fatty acids 18 : 2, 20 : 3 and 20 : 4.