Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. The diagnosis of pulmonary TB in general still relies on microscopic examination with a sputum smear for Acid Fast Basil (AFB). Microscopic examination to detect M. tuberculosis has high specificity, but the sensitivity is 35-70%. The sputum culture method is the gold standard for the diagnosis of pulmonary TB but requires a long time, which is 6-8 weeks. In recent years ICT (Immuno Chromatography Test) has been introduced to identify potential and useful TB antigens to help diagnose pulmonary TB. Antigens detected were ESAT-6, CFP-10, CFP-21, and MPT-64. This study aims to compare the results of the examination of specimens of suspect TB sputum with the ICT method and AFB. This study based on observational with analytic design cross-sectional on 56 samples of Stored Biological Material suspects TB that were examined by the AFB method and the ICT method (Cocktail Antigen). Statistical test results Fisher Exact (α = 0.05) showed no difference between microscopic examination of smear sputum and ICT (Cocktail Antigen) TB (p = 1,000). Nevertheless, the use of ICT reagents for the diagnosis of pulmonary TB still needs to be further investigated, especially to find out the exact causes of false positive and false negative reactions.
Vegetarian vegans are at risk of anemia, it maybe because the food intake lacks the nutritional components necessery for hemoglobin formation. The aims of this study is to determine of hemoglobin (Hb) concentration in vegetarian as an indication of suffering of anemia. The study was conducted on 57 vegetarian vegan people which was eligible by inclusioncriterion. The independent variables in this study were gender, age, and duration of being vegetarian, while the dependent variable was hemoglobin concentration. Hemoglobin concentrations of each subject was measured by Cyanmethehemoglobinmethod. Bivariate analyzed was done bychi square test and by descriptively bycomparing Hb concentration with reference value. There are four people (7.0%)out of the 57 subjects studied who had Hb concentrations below the reference value, consisting of one male and three female. The male and female subjects between the ages 12-25 years had the best mean Hb concentrations compared to others aged groups. T test showed no corralation between Hb concentrations with gender, age, and duration of being vegetarian (P ≥ 0.05).That may be due to the good-vegetarian nutritional intake.The result also show that long being vegetarian does not cause low Hb concentration, and refuse the hypothesis that it will cause low Hb concentration.