Water absorption ability is a critical property for starch-based materials used in absorbent and hemostatic applications. This study investigated the effects of plasma treatment and gamma irradiation on the water absorption behavior and physicochemical properties of modified potato starch powders. Three modified potato starch powders were examined: acetylated distarch phosphate (ADP), sodium chloride-modified ADP (MADP), and sodium carboxymethyl starch (SCS). Plasma treatment significantly increased the water absorption capacities of ADP, MADP, and SCS powders from 1021.9 +/- 55.1% to 1485.0 +/- 93.2%, from 1328.4 +/- 55.8% to 1489.8 +/- 68.5%, and from 1139.5 +/- 52.6% to 2000.1 +/- 73.7%, respectively. Fourier-transform infrared spectroscopy revealed increased carbonyl-related absorbance after plasma treatment, suggesting that plasma-induced surface oxidation enhanced hydrophilicity and promoted hydrogen bonding interactions with water molecules. In contrast, gamma irradiation produced starch-dependent effects. Water absorption in MADP decreased from 1328.4 +/- 55.8% to 1154.3 +/- 29.4%, whereas irradiated SCS powders exhibited increased water absorption from 1139.5 +/- 52.6% to 1663.0 +/- 10.8%. In contrast, water absorption of ADP powders was completely lost after gamma irradiation. The enhanced water absorption behavior of irradiated SCS powders may be associated with radiation-induced chain scission, increased exposure of hydrophilic carboxymethyl groups, and reduced particle size. Scanning electron microscopy further revealed reduced particle size in treated SCS powders, which may contribute to increased surface area and enhanced water absorption. Thermogravimetric and differential scanning calorimetry analyses demonstrated that gamma irradiation altered the thermal stability of the starches without significantly changing their crystalline structures. In addition, the pH values of all treated starch powders remained within the range of 5-7, which is generally considered suitable for biomedical-related material environments. These findings demonstrate that plasma treatment and gamma irradiation induce distinct physicochemical modifications in different starch structures and may provide useful strategies for developing starch-based absorbent materials with potential hemostatic and biomedical applications.

Platelet-rich plasma (PRP) is widely used in cosmetic and topical biomedical applications; however, conventional preparation methods rely heavily on centrifugation, which becomes operationally demanding when processing large blood volumes. In this study, a sedimentation-assisted strategy was investigated as an alternative to the initial centrifugation step for industrial-scale production of porcine PRP lyophilized powder. Whole blood anticoagulated with ACD-A was subjected to gravity sedimentation for 6–12 h, achieving >99.6% erythrocyte removal while maintaining a platelet recovery rate of >64%, comparable to conventional centrifugation. For large-volume batches (e.g., 100 L), this approach significantly reduced operator-intensive handling time. ACD-A outperformed other anticoagulants in preserving platelet integrity and preventing hemolysis during prolonged sedimentation. These findings demonstrate that gravity sedimentation represents a practical, scalable, and cost-effective alternative for the initial separation step in large-scale manufacturing of cosmetic-grade PRP raw material, with quality controlled by TGF-β1 concentration as the key release specification.

Cancer therapy requires advanced drug delivery systems capable of responding to complex tumor microenvironments through multiple environmental stimuli. In this study, a nanocarrier composed of chitosan (CS), Pluronic F127 (PF127), and silver nanoparticles (AgNP) was developed as a dual stimuli-responsive platform. AgNP were synthesized via a green approach using Ulva lactuca extract, while ibuprofen (IB) was employed as a model drug to evaluate the encapsulation and release performance of the nanocarrier. The structural and physicochemical properties of the system were characterized using FTIR, XRD, and DLS analyses. The nanocarrier exhibited an encapsulation efficiency of 76.6% and a drug loading content of 21.9%. In vitro release studies performed under different temperature (27 and 37 degrees C) and pH (4 and 7) conditions demonstrated dual stimuli-responsive release behavior. At pH 7, cumulative drug release increased from 43.3% at 27 degrees C to 57.7% at 37 degrees C, while release under acidic conditions remained substantially lower. Drug release kinetics were best described by the Higuchi model, indicating that diffusion was the predominant release mechanism. The biological response of the nanocomposite was evaluated using T47D breast cancer cells. CCK-8 and apoptosis assays revealed concentration- and time-dependent cytotoxic effects, with the highest apoptotic cell population observed after 48 h of incubation. Overall, the results demonstrate that the Ag/CS/PF127 nanocomposite exhibits dual pH-and thermo-responsive release characteristics and may serve as a promising platform for stimuli-responsive drug delivery applications.

BACKGROUND:Absorbable barbed sutures have been widely used for minimally invasive facial rejuvenation. However, clinical lifting performance varies due to differences in suture geometry, which remain insufficiently quantified. OBJECTIVE:The study aims to evaluate the effects of barb rotation angle, barb orientation, and pulling speed on the lifting performance and efficiency of the barbed sutures. The goal is to identify the optimized geometric design for facial lifting applications. METHODS:The barbs were fabricated with four rotation angles (30°, 45°, 90°, and 180°) and four orientations (Forward, Reverse, Forward-Reverse, and Reverse-Forward). The fabricated sutures were tested at 10, 50, and 100 mm/min. Maximum lifting and holding displacements and lifting efficiency were quantified. Statistical analysis was conducted using one-way ANOVA with Tukey's post hoc test (p < 0.05). RESULTS:Higher pulling speed increased maximum lift but reduced efficiency due to slippage. The maximum lift was at 100 mm/min (2.03 ± 0.08 mm), whereas the highest lifting efficiency was at 10 mm/min (42.7%). For the barb rotation angles, the 90° configuration showed the highest lifting efficiency (35.5%) and superior anchor stability (p < 0.05). Regarding the barb orientation, the Forward orientation produced the largest lift (1.72 ± 0.18 mm), while the Forward-Reverse orientation achieved the highest efficiency (35.5%). The optimized Forward-Reverse 90° configuration exhibited improved holding capacity and reduced slippage compared to a commercial barbed suture. CONCLUSION:Barb geometry critically affects the lifting performance of the suture. The Forward-Reverse 90° configuration shows the optimal lifting efficiency and stability, offering clinically relevant guidance for designing next-generation facial lifting sutures.

Transarterial embolization (TAE) is a minimally invasive endovascular therapy used for tumor devascularization, hemorrhage control, and the treatment of vascular malformations. In particulate TAE, embolic microspheres are delivered through microcatheters to occlude target vessels, and the clinical outcome strongly depends on the particle uniformity, deliverability, mechanical compliance, and intravascular biocompatibility. However, currently available biodegradable embolic materials often degrade too rapidly, leading to premature recanalization, and their acidic degradation products may induce local inflammatory responses. Therefore, degradable microspheres with tunable mechanical properties and a controllable degradation behavior are needed. In this study, polylactic acid/polycaprolactone (PLA/PCL) blend microspheres were fabricated using an emulsion solvent evaporation method with varying PLA/PCL ratios and systematically evaluated. Incorporation of PCL modulated microsphere surface morphology and improved particle deformability, while compression testing demonstrated a progressive reduction in apparent Young's modulus compared with neat PLA. Hemolysis and cytotoxicity assays confirmed that the P-(LA/CL)-73 formulation exhibited nonhemolytic behavior and good cytocompatibility. In addition, P-(LA/CL)-73 demonstrated enhanced thrombus formation, controlled and sustained degradation, and improved distal embolization performance in a 3D-printed in vitro vascular model. These findings suggest that P-(LA/CL) blend microspheres, particularly P-(LA/CL)-73, represent a promising biodegradable embolic platform with tunable mechanical and degradation properties for next-generation TAE applications.

Given the market for chronic wound care, where wound dressings are the key product, it should go without saying that providing satisfactory wound dressings is critical. A wide variety of wound dressings have been developed. Hydrogel has drawn attention as a potential wound dressing for chronic wounds. Hydrogel may absorb large amounts of water and up to 90% of biological fluids because it is a hydrophilic polymer. Nevertheless, there are still a number of unresolved issues with hydrogel, including frequent changing, the absence of real-time wound monitoring, maceration risk, and limited patient mobility. Several studies have attempted to overcome the limitations of hydrogel wound dressings. However, it is also possible that some problems are still unsolved or practically unproven. This review will discuss the limitations of the developed hydrogel dressings. We will also try to discuss prospective future projects and the strategies and tactics that have been investigated to circumvent the existing limitations.

The incorporation of volatile, thermally labile bioactive agents into porous calcium-deficient hydroxyapatite (CDHA; Ca9(HPO4)(PO4)5OH) biomaterials presents an inherent design challenge: thermal treatment enhances biomaterials integrity, however, it could risk degradation and loss of biological activity of the active phase. In this study, CDHA–clove oil (CEO) composites at a concentration of 10% wt. were loaded into the solution during the precipitation method, and subjected to two distinct temperature treatments at 70°C (CEO-70) and 100°C (CEO-100) to explicitly investigate this trade-off. Bruneian-Emmet-Teller (BET) surface area analysis detected that heating at 100°C resulted in a higher surface area (106.54 m2/g) compared to drying at 70°C (10.75 m2/g). While, Fourier-transform infrared (FTIR) spectroscopy showed a pronounced attenuation of CEO–associated vibrational bands after treatment at 100°C, consistent with partial loss or decomposition of the thermally sensitive bioactive components. Correspondingly, antibacterial assays demonstrated superior efficacy for the composites treated at 70°C despite its lower surface area. These results demonstrate that increased surface area does not necessarily translate to improved biological activity in proposed systems of incorporated volatile bioactives and establish a fundamental processing–function trade–off. Thus, temperature emerges as a critical design parameter governing the balance between material textural optimization and preservation of bioacitivity in hydroxyapatite–based composites.

Chitosan (CS) is a cationic linear polysaccharide rich in functional groups (-OH and -NH2) that enable diverse biochemical interactions, making it a promising candidate for the electrochemical detection of dopamine (DA), uric acid (UA), and ascorbic acid (AA). However, its poor electrical conductivity and limited thermal stability restrict its standalone performance, thus requiring the formation of composites with other materials. In this study, we report the synthesis of a chitosan/Fe3O4/graphene nanoplatelet (CS@Fe3O4/GNP) nanocomposite for the highly sensitive and selective electrochemical detection of DA, UA, and AA. The nanocomposite integrates the superior conductivity and high surface area of GNP, the electrocatalytic activity of Fe3O4 nanoparticles, and the biocompatibility and adhesive nature of chitosan. The CS@Fe3O4/GNP composite with various CS concentration (0.0625, 0.125, and 0.25%) was synthesized via a facile in-situ co-precipitation method. Electrochemical studies demonstrated that the 0.25% CS@Fe3O4/GNP-modified glassy carbon electrode (GCE) exhibited excellent detection performance toward DA, UA, and AA, with limits of detection (LOD) of 28.37 nM (range 30-488 nM), 566.27 nM (range 0.98-15.6 μM), and 26.54 μM (range 31.25-500 μM), respectively. The sensor also achieved recovery rates of 87.83-92.68% (DA), 82.41-91.61% (UA), and 88.83-95.04% (AA) in human blood serum samples.

Transarterial embolization (TAE) is a minimally invasive endovascular therapy used for tumor devascularization, hemorrhage control, and the treatment of vascular malformations. In particulate TAE, embolic microspheres are delivered through microcatheters to occlude target vessels, and the clinical outcome strongly depends on the particle uniformity, deliverability, mechanical compliance, and intravascular biocompatibility. However, currently available biodegradable embolic materials often degrade too rapidly, leading to premature recanalization, and their acidic degradation products may induce local inflammatory responses. Therefore, degradable microspheres with tunable mechanical properties and a controllable degradation behavior are needed. In this study, polylactic acid/polycaprolactone (PLA/PCL) blend microspheres were fabricated using an emulsion solvent evaporation method with varying PLA/PCL ratios and systematically evaluated. Incorporation of PCL modulated microsphere surface morphology and improved particle deformability, while compression testing demonstrated a progressive reduction in apparent Young's modulus compared with neat PLA. Hemolysis and cytotoxicity assays confirmed that the P(LA/CL)73 formulation exhibited nonhemolytic behavior and good cytocompatibility. In addition, P(LA/CL)73 demonstrated enhanced thrombus formation, controlled and sustained degradation, and improved distal embolization performance in a 3D-printed in vitro vascular model. These findings suggest that P(LA/CL) blend microspheres, particularly P(LA/CL)73, represent a promising biodegradable embolic platform with tunable mechanical and degradation properties for next-generation TAE applications.

In this work, a series of Cr3+-doped (3-tricalcium phosphate ((3-TCP) powders was synthesized by a wet co- precipitation method. It was shown that the dissolution of Cr3+ ions in the (3-TCP crystal structure is limited, resulting in the formation of a Cr2O3 secondary phase at higher substitution levels. The room-temperature and temperature-dependent photoluminescence excitation spectra, emission spectra, and photoluminescence decay curves of the samples were studied. Thermally stimulated luminescence, and afterglow decay spectral measurements were performed as well. All the synthesized materials exhibited broadband photoluminescence in the near-infrared region, ranging from approximately 680 to 900 nm, with a maximum centered at 760 nm. The strongest emission was detected for the sample with a doping level of 0.1 mol% Cr3+. Moreover, for the (3-TCP: 0.1 mol% Cr3+ sample, the afterglow after irradiation with X-rays can be detected for at least 8 h.

Background: Postoperative abdominal adhesions are a common and serious complication following abdominal surgery, often leading to chronic pain, bowel obstruction, or infertility. This study aimed to evaluate the efficacy of the new starch-based absorbable hemostatic agent and dressing, BioSight, in comparison with a predicate device (4DryField® PH) for the prevention of abdominal adhesions in a rat model. Methods: A total of 90 Sprague-Dawley rats were used to establish an intra-abdominal adhesion model and assigned to the BioSight, 4DryField® PH, or control group. Standardized injuries were created on the cecum and parietal peritoneum, followed by application of the designated materials. Animals were sacrificed at 2, 4, and 12 weeks for macroscopic adhesion scoring and histopathological evaluation. Adhesion area, adhesion strength, and tissue thickness were assessed using established scoring systems, and local healing was examined by H&E staining. All quantitative data were analyzed using one-way ANOVA. Conclusions: In a rat peritoneal adhesion model, BioSight exhibited pronounced anti-adhesion efficacy comparable to 4DryField® PH. Macroscopic evaluation showed consistently low adhesion scores (≤0.4) across all time points up to 12 weeks, while histological analysis confirmed reduced adhesion thickness, with BioSight displaying numerically lower values, particularly at early stages (251.3 ± 137.4 µm vs. 323.2 ± 174.6 µm at Week 2). This performance is attributed to rapid in situ hydrogel formation that provides effective temporary tissue separation, limits early fibrin deposition and inflammatory cell infiltration, and supports hemostasis. Importantly, the starch-based hydrogel exhibits a balanced biodegradation profile-persisting long enough to protect injured tissues during the critical inflammatory and fibroproliferative phases, yet undergoing complete enzymatic resorption thereafter without adverse tissue reactions. Collectively, these results highlight the anti-adhesion functionality of BioSight and support the clinical potential of plant-derived starch-based bioresorbable surgical adjuncts.

INTRODUCTION:Polylactic acid can be classified into poly(L-lactic acid) (PLLA) and poly(D,L-lactic acid) (PDLLA) according to their stereoisomeric structures, and both are widely used as dermal fillers for soft tissue augmentation. Although the clinical efficacy of commercially available PLLA- and PDLLA-based fillers has been well established, variations in their physicochemical properties may lead to differences in handling characteristics and clinical performance. A systematic comparison of these properties among different PLA-based fillers remains limited. MATERIALS AND METHODS:In this study, the physicochemical characteristics of three PDLLA-based fillers (AestheFill, NeoFilera, and Juvelook) and one PLLA-based filler (Sculptra) were evaluated. The analyses included functional group identification, particle morphology and size distribution observation, reconstitution time measurement, osmotic pressure determination, and viscosity assessment. RESULTS:AestheFill and NeoFilera exhibited similar profiles in terms of functional groups, size distribution, osmotic pressure, and viscosity, while NeoFilera and Juvelook showed comparable particle morphologies. Sculptra displayed distinct particle morphology and viscosity, likely attributable to its PLLA composition, yet showed similarities with Juvelook in functional group identification and osmotic pressure. Additionally, the reconstitution times of Sculptra, NeoFilera, and Juvelook were significantly shorter than that of AestheFill. CONCLUSIONS:Although the direct correlation between physicochemical characteristics and clinical outcomes warrants further investigation, this comparative analysis provides clinicians with a clearer understanding of the material properties of PLA-based dermal fillers and may assist in the informed selection of appropriate products for individual patients.

This study explores the effects of thermal treatment on X-ray-induced processes in magnesium whitlockite (MgWH; Ca18Mg2(HPO4)2(PO4)12) prepared via a dissolution-precipitation reaction under hydrothermal conditions. Powder X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM) analyses reveal enhanced crystallinity after annealing of the as-prepared material at 600 degrees C. Annealing also minimises luminescence quenching effects, leading to increased luminescence intensity. After Xray irradiation, paramagnetic species are generated, which can be detected by electron paramagnetic resonance (EPR) spectroscopy. Based on EPR spectra simulations, atomic hydrogen (H0) centres and PO42- radicals are identified in Mg-WH. The annealing of the radiation-induced paramagnetic centres is partially correlated with thermally stimulated luminescence (TSL) processes. The obtained results highlight the importance of thermal treatment for controlling the types of charge traps and enhancing the luminescence efficiency of the material.

When an orthokeratology (ortho-k) lens contacts the ocular surface, tear film components such as lipids and proteins rapidly adsorb onto the lens, which may increase friction and contribute to discomfort if not properly removed. Polysaccharides have been reported to reduce protein deposition and improve lubrication, prompting the investigation of alginate acid and lambda-carrageenan in modulating the tribological properties of ortho-k lenses. An in vitro tribological property analysis of ortho-k lenses and protein adsorption and desorption analyses were carried out to investigate the lubricating ability of alginate acid and carrageenan. Zeta potential and turbidity analyses were further conducted to examine potential interactions between polysaccharides and tear film proteins. Tear film proteins significantly increased the friction coefficient of the ortho-k lens, whereas the addition of alginate acid and carrageenan markedly reduced friction. Electrostatic interaction and polysaccharide-protein complex formation were identified as possible mechanisms underlying these effects. These results demonstrate that alginate acid and carrageenan can modify the tribological and interfacial behavior of ortho-k lenses in protein-rich environments, suggesting their potential application in reducing friction-related complications in ortho-k lens wearers.

Myopia patients wear rigid gas-permeable contact lenses during the day to achieve normal vision, but they might feel uncomfortable, since they are made of hard materials that can cause inappropriate friction and adhesion. These forces affect the biological tissues of the cornea and eyelid. In this study, an in vitro rigid gas-permeable contact lens friction testing method was established to mimic the friction between the eyelid and the rigid contact lens. The lens was rubbed against a gelatin membrane to investigate the tribological properties of artificial tear, saline, and two kinds of care solutions using a dedicated experimental setup. The viscosity, pH value, and surface tension of each lubricant was also analyzed. The friction coefficient of the artificial tear solution was the highest: 0.18 for its static friction and 0.09 for its dynamic friction. In contrast, polysaccharide-containing care solution demonstrated the lowest friction coefficient. The viscosity of artificial tear solutions ranged from 0.97 ± 00 to 1.15 ± 0.16 mPa·s, when the shear rate was increased from 19.2 to 192 1/s, while it ranged from 2.26 ± 1.12 to 2.91 ± 0.00 for polysaccharide-containing care solution. Although the physical–chemical properties of various lubricants could not explain the distinct tribological outcomes, the in vitro tribological testing method for rigid gas-permeable lenses was successfully established in this study.

Poly(L-lactic acid) (PLLA) and poly(D,L-lactic acid) (PDLLA) particles have been applied as dermal fillers for soft-tissue augmentation because they can induce foreign-body reactions, resulting in fibroblast proliferation and collagen formation. Although PLLA and PDLLA fillers are safe and biocompatible, clinical complications such as nodules and granulomas have been reported, possibly due to incomplete reconstitution. PDLLA particles were prepared via emulsification in this study, and three stirring speeds were investigated when adding PDLLA into carboxymethyl cellulose solution. The particle size, molecular weight of PDLLA, optical rotation, pH value, osmotic pressure, and reconstitution time were analyzed. A rabbit dorsal ear model was established to evaluate the soft-tissue augmentation of a commercial PDLLA filler. The results demonstrated that the stirring speed affected the particle size, but not other physical-chemical properties of the PDLLA particles. All the PDLLA particles were reconstituted in less than 7 min, which is faster than the process for the other commercial PDLLA dermal filler products. In addition, the PDLLA particles could induce inflammation and fibroblast proliferation. Although the PDLLA particles generated in this study have not yet been investigated in vivo, the results demonstrated here suggest their potential for application as dermal fillers.

Antibacterial alloys are widely applied to reduce the incidence of medical-implant-associated infection. Copper (Cu) and silver (Ag) are commonly used in antibacterial alloys; however, rare earth elements, such as Cerium (Ce), are now gaining attention because their low trace is sufficient for killing bacteria. Accordingly, the antibacterial activity of Copper48-Silver48-Cerium4 (CuAgCe4) alloys with different crystalline structures was investigated. The immersion approach was employed for alloys cultured with Escherichia coli, and a direct contact method was used for alloys cultured with Staphylococcus aureus. Surface morphology was observed when alloys were made, and the crystalline structures of alloys were examined before and after being cultured with bacteria. The immersion method revealed that all the CuAgCe4 alloy samples could inhibit the growth of Escherichia coli, and the crystallized structures were distorted after the alloys were cultured with bacteria. Conversely, the direct contact approach showed the crystalline structures of CuAgCe4 alloys remained unchanged after the culture with Staphylococcus aureus, thereby indicating that the antibacterial activity did not correspond to the crystalline structures. Despite the lack of clarity surrounding the possible antibacterial mechanisms of CuAgCe4 alloy, the current findings demonstrate the potential antibacterial effects of CuAgCe4 alloy in medical implants.

Peri-implantitis is a common complication characterized by inflammation in tissues surrounding dental implants due to plaque accumulation, which can lead to implant failure. While air flow abrasive treatment has been found to be effective for debriding implant surfaces, little is known about the factors that affect its cleaning capacity. This study systematically examined the cleaning capacity of air powder abrasive (APA) treatment with β-tricalcium phosphate (β-TCP) powder, using various powder jetting strengths and different particle sizes. Three sizes of β-TCP powder (S, M, and L) were prepared, and different powder settings (low, medium, and high) were tested. The cleaning capacity was determined by quantifying ink removal, which simulated biofilm removal from the implant surfaces at different time points. The results of the systematic comparisons showed that the most efficient cleaning of implant surfaces was achieved using size M particles with medium setting. Additionally, the amount of powder consumed was found to be critical to cleaning efficiency, and the implant surfaces were altered in all tested groups. These systematically analyzed outcomes may provide insights into the development of potential non-surgical strategies for treating peri-implant diseases.

Abstract Background As an increasing population has received different kinds of COVID-19 vaccines, many ocular adverse events have been reported. The objective of this study is to evaluate the association between COVID-19 vaccines and the activation of herpetic keratitis. Methods Patients presented with any type of herpetic keratitis after mixing and matching COVID-19 vaccinations was enrolled. A descriptive analysis of the demographics, clinical history and ocular presentation in these patients was performed. Results A total of 16 patients including 6 (37.5%) males and 10 (62.5%) females receiving COVID-19 vaccines between 2021 to 2022 were included. The mean age at evaluation was 59.4 ± 15.4 years. Of the 16 patients, 9 (56.3%), 2 (12.5%), 5 (31.3%) of them presented with stromal keratitis, epithelial keratitis and herpes zoster ophthalmicus respectively. Among 8 recurrent cases, the COVID-19 vaccine-related attacks presented severer in 1 (12.5%) patient, equally severe in 2 (25%) patients, and less severe in 5 (62.5%) patients compared to previous infection. Interestingly, 6 (37.5%) patients developed an initial symptomatic herpetic keratitis attack after mixing and matching (COVID-19) vaccination. All of them received oral famciclovir with or without topical steroid eyedrops soon after diagnosis. The majority of them reported no further complication. Conclusions In conclusion, this study demonstrated a possible association between COVID-19 vaccination and primary herpetic infection or reactivation. Early diagnosis and appropriate management help reduce the severity of the disease.

Human blood-derived topical therapies have been a boon to clinicians in recent decades. Autologous serum (AS) and platelet-rich plasma (PRP) are enriched in epitheliotropic growth factors that are essential in corneal wound healing. Unlike AS, PRP is based on a differential centrifugation system, yielding more platelet-derived growth factors. Autologous conditioned serum (ACS) not only preserves the preparation of AS and PRP, but also focuses on immune-modulating properties, which are important in inflammatory diseases. The lack of standardized protocols and high preparation costs are limitations for the clinical application of ACS. This video experiment demonstrates a standard operating procedure for preparing modified autologous conditioned serum (mACS) eye drops. First, glycerol was added into heparin syringes as the blood cell stabilizer during hypoxic incubation. To activate the blood cells, a 4 h incubation at 37 °C was initiated. Then, the blood samples were centrifuged at 3,500 × g for 10 min at room temperature. After filtration of the supernatant through a 0.22 µm filter, the mACS eye drops were fully prepared. A tentative try-out of the therapeutic effect of mACS showed that it may have competitive advantages over conventional AS in the corneal wound healing in ex vivo mouse eyes. The AS used in this study was prepared according to published studies and the clinical practice in our hospital. Therefore, the efficacy of mACS on ocular surface diseases could be evaluated in future research through in vivo animal studies and clinical trials.