Cr(VI) widely exists in the environment and has highly toxic, carcinogenic and mutagenic effects on all organisms. Physical/chemical methods to remove chromium pollution are economically expensive and have disadvantages like high reagent consumption, energy requirements and so on, while bioremediation is an eco-friendly, simple and cost-effective way. In this study, a novel Cr(VI)-reducing strain, Microbacterium sp. NEAU-W11, was reported, and its reduction mechanism was investigated. Microbacterium sp. NEAU-W11 could effectively degrade Cr(VI) under the conditions of pH 7–10, 15–35 °C, and the coexistence of metal pollutants such as Pb and Ni, etc. In addition, both Fe3+ and Cu2+ could improve the reducing ability of strain NEAU-W11, and glucose and lactose as electron donors also had promoting effect. Heat treatment of resting cells confirmed that chromium removal was not biological sorption but biological reduction. The active reductase of strain NEAU-W11 to chromium(VI) mainly existed in the cell cytoplasm, which is the first report in the genus Microbacterium. Micro-characterization of strain NEAU-W11 and the reduction products identified the reduction products as Cr(III)-ligand complexes bound to extracellular polymeric substances (EPS). Collectively, this study systematically investigated the degradation mechanism of Microbacterium sp. NEAU-W11 and the distribution of degradation product Cr(III), providing a new reduction mechanism for the genus Microbacterium, providing a new perspective for a comprehensive understanding of the degradation and transport of chromium by bacteria, and providing theoretical reference for the migration of metal ions in environmental governance.

Rice direct seeding technology has been considered as a promising alternative to traditional transplanting because of its advantages in saving labor and water. However, the poor emergence and seedling growth caused by chill stress are the main bottlenecks in wide-scale adoption of direct-seeded rice in Heilongjiang Province, China. Here, we found that natural plant growth regulator guvermectin (GV) effectively improved rice seed germination and seedling growth under chilling stress. Results from 2 year field trials showed that seed-soaking with GV not only enhanced the emergence rate and seedling growth but also increased the panicle number per plant and grain number per panicle, resulting in 9.0 and 6.8% increase in the yield of direct-seeded rice, respectively. Integrative physiological, transcriptomic, and metabolomic assays revealed that GV promoted seed germination under chilling stress mainly by enhancing the activities of α-amylase and antioxidant enzymes (superoxide dismutase, peroxidase, and catalase), increasing the contents of soluble sugar and soluble protein, improving the biosynthesis of glutathione and flavonoids, as well as activating gibberellin-responsive transcription factors and inhibiting the abscisic acid signaling pathway. These findings indicate that seed-soaking with GV has good potential to improve seedling establishment and yield of direct-seeded rice even under chilling stress.

Purple Passion fruit (Passiflora edulis) is widely cultivated in many regions of southern China as an edible tropical fruit with excellent nutritional value and high economic value. In July 2021, postharvest fruit rot was observed on 20-25% of purple passion fruit in several fruit markets of Dehong City in Yunnan Province. Symptoms on infected fruits were irregular, pink-brown, soft, and water-soaked lesions, which enlarged and formed sunken patches with time as well as producing a small amount of white mycelium. To isolate the causal organism, five diseased fruits were collected from different fruit markets. A conidial mass from an individual sorus observed on an infected fruit was isolated and cultured on potato dextrose agar (PDA) supplemented with 50 μg ml-1 of streptomycin, and five fungal isolates were obtained. These isolates were morphologically similar and produced pale pink colonies on PDA for 7 days containing several conidiophores with abundant conidia. Mycelia were hyaline, 2 µm in diameter, and conidiophores were simple or branched (100 to 286 × 1.5 to 2.5 µm, n=50). Conidia were pyriform, ovate, with papillary protuberances at one end. Almost all conidia were two-celled and single-septate (5.8 to 9.1 × 1.7 to 4.9 µm, n=50). The morphology of the fungi resembled Trichothecium roseum as reported previously (Inácio et al. 2011). To further confirm the fungal species, isolate PASF4 was selected for molecular identification by amplifying and sequencing the ribosomal internal transcribed spacer (ITS) and large subunit (LSU) genes. Primers and PCR amplification were described by Fell et al. (2000). Results showed that both the ITS (GenBank accession OL336243) and LSU (OL336242) gene sequences had 100% similarity to T. roseum in NCBI database (MH856757 and MH868278). Maximum likelihood tree was constructed using MEGA 7 (Felsenstein, 1981) based on concatenated sequences (ITS and LSU) of isolate PASF4 and reference strains. Phylogenetic analysis showed that isolate PASF4 belonged to T. roseum clade. Based on morphological characteristics and phylogenetic analysis, isolate PASF4 was identified as T. roseum (Inácio et al. 2011). To confirm their pathogenicity, healthy purple passion fruits (cv. Tainong-1) were disinfected in 0.5% NaClO solution for 2 min, and then washed with sterile water. After wounding with a sterile needle, the fruits were inoculated by placing mycelium agar plugs on the wounds, and mock inoculation with mycelium-free PDA plugs served as control. Five fruits were used in each treatment. All fruits were maintained in plastic boxes at 25 °C. Disease symptoms appeared after inoculation for 4-7 days on all inoculated fruits, which were similar to those observed in fruit markets. No symptoms were observed on fruits used as control. The Trichothecium isolates were re-isolated from symptomatic fruits thus fulfilling Koch's postulates. Trichothecium roseum has been reported to cause fruit rot of tomato, apple and orange in Pakistan (Hamid et al., 2014) and fruit rot of pepper in China (Lin et al., 2016). To our knowledge, this is the first report of T. roseum causing fruit rot on purple passion fruit worldwide, and these data will provide useful information for developing effective control strategies.

A bacterial strain, Gram-stain negative, rod-shaped, aerobic and cellulose-degrading, designated NEAU-DD11T, was isolated from rhizosphere soil of rice collected from Northeast Agricultural University in Harbin, Heilongjiang Province, North-east China. Base on 16S rRNA gene sequence analysis, strain NEAU-DD11T belongs to the genus Massilia and shared high sequence similarities with Massilia phosphatilytica 12-OD1T (98.46%) and Massilia putida 6NM-7 T (98.41%). Phylogenetic analysis based on the 16S rRNA gene and whole genome sequences indicated that strain NEAU-DD11T formed lineage related to M. phosphatilytica 12-OD1T and M. putida 6NM-7 T. The major fatty acids of the strain were C16:0, C17:0-cyclo and C16:1ω7c. The respiratory quinone was Q-8. The polar lipids profile of the strain showed the presence of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified polar lipid and an unidentified phospholipid. In addition, the digital DNA-DNA hybridization values between strain NEAU-DD11T and M. phosphatilytica 12-OD1T and M. putida 6NM-7 T were 45.4 and 35.6%, respectively, which are lower than the accepted threshold value of 70%. The DNA G + C content of strain NEAU-DD11T was 66.2%. The whole genome analysis showed the strain contained carbohydrate enzymes such as glycoside hydrolase and polysaccharide lyase, which enabled the strain to have the function of degrading cellulose. On the basis of the phenotypic, genotypic and chemotaxonomic characteristics, we conclude that strain NEAU-DD11T represents a novel species of the genus Massilia, for which the name Massilia cellulosiltytica sp. nov. is proposed. The type strain is NEAU-DD11T (= CCTCC AB 2019141 T = DSM 109721 T).

A novel rhizobacterium, designated strain NEAU-GH312T, with antibacterial activity against Ralstonia solanacearum was isolated from rhizosphere soil of rice (Heilongjiang Province, PR China) and characterized with a polyphasic approach. Cells of strain NEAU-GH312T were Gram-stain-negative, aerobic, non-spore-forming, motile with peritrichous flagella and rod-shaped. Colonies were light orange, convex and semi-translucent on Reasoner's 2A (R2A) agar after 2 days of incubation at 28 °C. Growth was observed on R2A agar at 10-40 °C, pH 4.0-8.0 and with 0-5 % (w/v) NaCl. The respiratory quinone was ubiquinone Q-8. The major cellular fatty acids of strain NEAU-GH312T were C16 : 1  ω7c and/or C16 : 1  ω6c, C16 : 0 and C18 : 1  ω7c and/or C18 : 1  ω6c. The main polar lipids were phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Phylogenetic analyses confirmed the well-supported affiliation of strain NEAU-GH312T within the genus Massilia, close to the type strains of Massilia arvi THG-RS2OT (98.7 %), Massilia norwichensis NS9T (98.7 %) and Massilia kyonggiensis TSA1T (98.6 %). Strain NEAU-GH312T had a genome size of 6.68 Mb and an average DNA G+C content of 66.3 mol%. Based on the genotypic, phenotypic and chemotaxonomic data obtained in this study, strain NEAU-GH312T could be classified as representative of a novel species of the genus Massilia, for which the name Massilia rhizosphaerae sp. nov. is proposed, with strain NEAU-GH312T (=DSM 109722T=CCTCC AB 2019142T) as the type strain.

A novel actinobacterium, designated strain NEAU-AAG5T, was isolated from sandy soil collected from Niuwang island in Sanya, Hainan Province, PR China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-AAG5T belongs to the genus Actinomadura and shared highest sequence similarity with Actinomadura macra NBRC 14102T (98.8 %). Strain NEAU-AAG5T grows at 20-40 °C (optimum, 28 °C), pH 6-10 (optimum, pH 7) and has NaCl tolerance of 0-3 %. The menaquinones were identified as MK-9(H4) (4.2 %), MK-9(H6) (49.2 %) and MK-9(H8) (46.5 %). The major fatty acids were C16 : 0 (31.4 %), 10-methyl C18 : 0 (21.3 %) and C18 : 1  ω9c (15.7 %). The polar lipids were diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositolmannoside, phosphatidylglycerol and phosphoglycolipid. The genomic DNA G+C content of strain NEAU-AAG5T based on whole genome sequences was 72.8 mol%. Digital DNA-DNA hybridization between strain NEAU-AAG5T and its closest phylogenetic neighbour, A. macra NBRC 14102T, resulted in similarity value of 28.0 % (<70 %). Additionally, the average nucleotide identity was 84.2 % for A. macra NBRC 14102T. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-AAG5T can be characterized to represent a novel species of the genus Actinomadura, for which the name Actinomadura litoris sp. nov. is proposed. The type strain is NEAU-AAG5T (=JCM 33456T=CCTCC AA 2019043T).

Enterobacter asburiae is the causal agent of rice bacterial palea browning disease. Here, we report the complete genome of E. asburiae strain SD4L, which represents the first whole genome sequence of an isolate from rice seedlings in China. The assembled genome consisted of two contigs, with a circular chromosome of 4,574,166 bp, and a plasmid of 85,271 bp, respectively. This complete genome will provide a valuable resource for further studies on bacterial palea browning worldwide.

Microorganisms related to plant roots are vital for plant growth and health and considered to be the second genome of the plant. When the plant is attacked by plant pathogens, the diversity and community structure of plant-associated microbes might be changed. The goal of this study is to characterize differences in root-associated endophytic actinobacterial community composition and antifungal activity between Fusarium wilt diseased and healthy cucumber and screen actinobacteria for potential biological control of Fusarium wilt of cucumber. In the present research, three healthy plants (also termed "islands") and three obviously diseased plants (naturally infected by F. oxysporum f. sp. cucumerinum) nearby the islands collected from the cucumber continuous cropping greenhouse were chosen as samples. Results of culture-independent and culture-dependent analysis demonstrated that actinomycetes in the healthy roots were significantly more abundant than those of diseased roots. Moreover, there were seven strains with antifungal activity against F. oxysporum f. sp. cucumerinum in healthy cucumber roots, but only one strain in diseased cucumber roots. Out of these eight strains, the isolate HAAG3-15 was found to be best as it had the strongest antifungal activity against F. oxysporum f. sp. cucumerinum, and also exhibited broad-spectrum antifungal activity. Thus, strain HAAG3-15 was selected for studying its biocontrol efficacy under greenhouse conditions. The results suggested that the disease incidence and disease severity indices of cucumber Fusarium wilt greatly decreased (p < 0.05) while the height and shoot fresh weight of cucumber significantly increased (p < 0.05) after inoculating strain HAAG3-15. On the basis of morphological characteristics, physiological and biochemical properties and 100% 16S ribosomal RNA (rRNA) gene sequence similarity with Streptomyces sporoclivatus NBRC 100767T, the isolate was assigned to the genus Streptomyces. Moreover, azalomycin B was isolated and identified as the bioactive compound of strain HAAG3-15 based on analysis of spectra using a bioactivity-guided method. The stronger antifungal activity against F. oxysporum f. sp. cucumerinum, the obvious effect on disease prevention and growth promotion on cucumber seedlings in the greenhouse assay, and the excellent broad-spectrum antifungal activities suggest that strain HAAG3-15 could be developed as a potential biocontrol agent against F. oxysporum f. sp. cucumerinum used in organic agriculture. These results suggested that the healthy root nearby the infected plant is a good source for isolating biocontrol and plant growth-promoting endophytes.

Rice is used as a staple food in different areas of world, especially in China. In recent years, rice seedlings have been affected seriously by symptoms resembling bacterial palea browning (BPB) in Heilongjiang Province. To isolate and identify the pathogenic bacteria responsible for the disease, 40 bacterial strains were isolated from diseased rice seedlings collected from the four major accumulative-temperature zones of rice fields cultivated in Heilongjiang Province, and these were identified as 13 species based on morphological characteristics and 16S ribosomal RNA (rRNA) gene sequences. Inoculation of all the isolates on healthy rice seedlings showed that the nine Enterobacter cloacae isolates were the pathogens causing typical symptoms of BPB, including yellowing to pale browning, stunting, withering, drying, and death. Moreover, the nine E. cloacae isolates could also cause symptoms of bacterial disease on the seedlings of soybean (Glycine max), maize (Zea mays L.), and tomato (Solanum lycopersicum). Phylogenetic analysis based on the 16S rRNA gene sequences and phenotypic and biochemical characteristics indicated that these nine pathogenic isolates were E. cloacae. In addition, analysis of the sequences of four housekeeping genes (rpoB, gyrB, infB, and atpD) from the selected strain SD4L also assigned the strain to E. cloacae. Therefore, E. cloacae is the pathogen causing disease of rice seedlings in Heilongjiang Province, which we propose to classify as a form of BPB. To the best of our knowledge, this is the first study to identify E. cloacae as a causal agent of BPB in rice.

A Gram-stain-positive, non-motile, creamy-white actinobacterium, which has an elementary branching rod-coccus life cycle was isolated from the rhizosphere soil of rice (Oryza sativa L.) collected from Northeast Agricultural University in Harbin, Heilongjiang province, north-east PR China, and its taxonomic status was examined by using a polyphasic approach. Results from the 16S rRNA gene sequence study showed that the isolate, designated strain NEAU-CX67T, belonged to the genus Rhodococcus and formed a cluster with Rhodococcus maanshanensis DSM 44675T, Rhodococcus kronopolitis NEAU-ML12T and Rhodococcus tukisamuensis JCM 11308T (98.3, 98.1 and 97.7% gene sequence similarity, respectively). The major fatty acids were C16 : 0, 10-methyl C18 : 0, C18 : 1 ω9c and C16 : 1 ω7c. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major isoprenoid quinone was MK-8(H2). Whole-cell hydrolysates contained meso-diaminopimelic acid. Arabinose, galactose and ribose were detected as diagnostic sugars from whole-cell hydrolysates. Mycolic acids were detected. The genomic DNA G+C content of strain NEAU-CX67T was 64.6 mol%. Strain NEAU-CX67T exhibited low average nucleotide identity and digital DNA-DNA hybridization values with R. maanshanensis DSM 44675T (92.1 and 45.4 %) and R. tukisamuensis JCM 11308T (81.9 and 24.4 %). On the basis of results of phylogenetic, genotypic, physiological and chemotaxonomic analysis, strain NEAU-CX67T is considered to represent a novel species of the genus Rhodococcus for which the name Rhodococcus oryzae sp. nov. is proposed. The type strain is NEAU-CX67T (=DSM 107701T=CCTCC AB 2018233T).

A novel actinobacterium, designated strain NEAU-C40T, was isolated from the rhizosphere soil of rice (Oryza sativa L.) collected from Northeast Agricultural University in Harbin, Heilongjiang province, north-east PR China, and was characterized using a polyphasic approach. On the basis of results of 16S rRNA gene sequence analysis, strain NEAU-C40T belongs to the genus Streptomyces, and shares highest sequence similarities with Streptomyces polygonati CGMCC 4.7237T (97.9%) and Streptomyces abietis DSM 42080T (97.9%). Morphological and chemotaxonomic characteristics of the strain also supported its assignment to the genus Streptomyces. Cell walls contained ll-diaminopimelic acid and the whole-cell hydrolysates were glucose, rhamnose and ribose. The major menaquinones were identified as MK-9(H8) and MK-9(H6). The major fatty acids were iso-C16:0, anteiso-C15:0, C16:0 and anteiso-C17:0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannoside and an unidentified lipid. The genomic DNA G+C content of strain NEAU-C40T was 71.8 mol%. Moreover, multilocus sequence analysis based on five other housekeeping genes (atp D, gyr B, rpo B, rec A and trp B) and the low level of DNA-DNA relatedness allowed the isolate to be differentiated from its most closely related strains. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-C40T can be characterized to represent a novel species of the genus Streptomyces, for which the name Streptomyces oryziradicis sp. nov. is proposed. The type strain is NEAU-C40T (=DSM 107943T=CCTCC AA 2018038T).

HomePlant DiseaseVol. 103, No. 7First Report of Fusarium incarnatum-equiseti Species Complex Causing Fruit Rot on Muskmelon (Cucumis melo) in China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Fusarium incarnatum-equiseti Species Complex Causing Fruit Rot on Muskmelon (Cucumis melo) in ChinaP. Cao, C. Li, W. Xiang, X. Wang, and J. ZhaoP. CaoKey Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Xiangfang District, Harbin 150030, P.R. China, C. LiKey Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Xiangfang District, Harbin 150030, P.R. China, W. XiangKey Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Xiangfang District, Harbin 150030, P.R. ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China, X. Wang†Corresponding authors: X. Wang; E-mail Address: wangneau2013@163.com and J. Zhao; E-mail Address: guyan2080@126.comKey Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Xiangfang District, Harbin 150030, P.R. China, and J. Zhao†Corresponding authors: X. Wang; E-mail Address: wangneau2013@163.com and J. Zhao; E-mail Address: guyan2080@126.comhttp://orcid.org/0000-0003-1250-2756Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Xiangfang District, Harbin 150030, P.R. ChinaAffiliationsAuthors and Affiliations P. Cao1 C. Li1 W. Xiang1 2 X. Wang1 † J. Zhao1 † 1Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Xiangfang District, Harbin 150030, P.R. China 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P.R. China Published Online:24 Apr 2019https://doi.org/10.1094/PDIS-09-18-1603-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Muskmelon (Cucumis melo L.) is an economically important fruit crop in China. In September 2018, fruit rot was observed on approximately 20% of muskmelon fruits in Harbin, Heilongjiang Province, China. Brown water-soaked lesions were observed on the fruit side in contact with soil initially, which gradually extended to most of or the entire fruit. Internal decay was observed with white to dark brown mycelium on the fruit surface. Diseased muskmelon tissues were surface disinfested with 1% NaOCl for 3 min, 70% ethanol for 10 s, and then washed three times with sterile water. The disinfested tissues were cut into 1-cm pieces and placed on potato dextrose agar (PDA) amended with streptomycin sulfate (50 mg/liter) and incubated at 25°C for 1 week. Isolations were done on 10 fruit fragments, and the same fungus was obtained. The cultures were purified using the hyphal-tip technique and used for morphological and molecular analyses. Morphological characteristics were observed on 1-week-old PDA cultures grown at 28°C. The aerial mycelium changed from white to light yellow, and the back of the plate turned pale brown with time. Microconidia were single celled, hyaline, nonseptate, ovoid, and 8.5 to 10.6 × 3.2 to 4.4 μm. Hyaline macroconidia (mostly three-septate) were slightly curved at the apex and ranged from 19.4 to 35.2 × 3.8 to 7.7 μm. Chlamydospores with thick, roughened walls were abundant in clumps or chains, ellipsoidal or subglobose. Primers ITS1/ITS4 (White et al. 1990) and EF-1/EF-2 (O'Donnell et al. 2000) were used to amplify and sequence the internal transcribed spacer (ITS1-5.8S-ITS2) and translation elongation factor 1-α (TEF1) region. ITS and TEF1 gene sequences were deposited in NCBI GenBank nucleotide database with accession numbers MH910492 and MH920853, respectively. BLASTn analysis showed 99% nucleotide sequence identity with Fusarium incarnatum-equiseti species complex (FIESC). The TEF1 gene sequence of isolate NEAU-TG1 was compared with sequences in the FUSARIUM-ID database (Geiser et al. 2004), which indicated that the pathogen was most closely (99% identity) related to phylogenetic species within the FIESC. Pathogenicity of these isolates was confirmed by following Koch's postulates. Ten muskmelon fruits of cultivar Xiangfei were surface disinfested with 2% NaOCl for 2 min and rinsed with sterile distilled water three times. Spores were produced on PDA for 7 days at 28°C and washed with sterile distilled water; the concentrations were adjusted to 1 × 106 spores/ml using a hemocytometer. Ten muskmelon fruits were inoculated by injecting the suspension (2 ml, 1 × 106 spores/ml) and covered with plastic bags for 24 h, and another 10 surface-disinfested fruits treated with sterile distilled water were used as a control. The fruits were placed in a humidified chamber (>95% relative humidity) at 25°C for 48 h after inoculation and kept in a growth chamber at 25°C with 12-h day/night cycle for 8 days. All inoculated fruits showed symptoms identical to those observed in the field. No disease occurred on the controls. The pathogen was reisolated from diseased fruits, and species identification was confirmed by the morphological and molecular method described above. Members of the FIESC can cause disease on plants, such as Allium cepa, Festuca arundinacea, Morchella importuna, and Cucumis trigonus. Furthermore, the members of the FIESC have been reported to produce type A and B trichothecene mycotoxins that cause toxicosis in humans and animals (Leslie and Summerell 2006; O'Donnell et al. 2009). To our knowledge, this is the first report of fruit rot caused by a member of the FIESC on muskmelon in China. This pathogen presents a threat to muskmelon production, and its accurate identification is necessary to develop effective management strategies.The author(s) declare no conflict of interest.References:Geiser, D. M., et al. 2004. Eur. J. Plant Pathol. 110:473. https://doi.org/10.1023/B:EJPP.0000032386.75915.a0 Crossref, ISI, Google ScholarLeslie, J. F., and Summerell, B. A. 2006. The Fusarium Laboratory Manual. Blackwell Publishing, Oxford, U.K. https://doi.org/10.1002/9780470278376 Crossref, Google ScholarO'Donnell, K., et al. 2000. Proc. Natl. Acad. Sci. U.S.A. 97:7905. https://doi.org/10.1073/pnas.130193297 Crossref, ISI, Google ScholarO'Donnell, K., et al. 2009. J. Clin. Microbiol. 47:3851. https://doi.org/10.1128/JCM.01616-09 Crossref, ISI, Google ScholarWhite, T. J., et al. 1990. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA. Crossref, Google ScholarFunding: Funding was provided by the National Natural Youth Science Foundation of China (grant no. 31701858).The author(s) declare no conflict of interest.DetailsFiguresLiterature CitedRelated Vol. 103, No. 7 July 2019SubscribeISSN:0191-2917e-ISSN:1943-7692 DownloadCaptionApple cultivar Joya Cripps Red lesions caused by Colletotrichum fructicola (Nodet et al.). Photo credit: P. Nodet. Symptoms of Lotus powdery mildew caused by Erysiphe takamatsui (Zhou et al.). Photo credit: C. Liang. Symptoms of tar spot (Phyllachora maydis) on maize leaves (Dalla Lana et al.). Photo credit: F. Dalla Lana. Metrics Article History Issue Date: 20 Jun 2019Published: 24 Apr 2019First Look: 11 Feb 2019Accepted: 5 Feb 2019 Page: 1768 Information© 2019 The American Phytopathological SocietyFundingNational Natural Youth Science Foundation of ChinaGrant/Award Number: 31701858KeywordsFusarium incarnatum-equiseti species complexfruit rotmuskmelonThe author(s) declare no conflict of interest.Cited byFirst Report of Fusarium incarnatum-equiseti Species Complex Causing Fruit Rot on Muskmelon in TaiwanAhmed Namisy, Mohamed Rakha, Wei-Che Hsu, and Wen-Hsin Chung12 February 2023 | Plant Disease, Vol. 107, No. 2Occurrence of Neopestalotiopsis clavispora Causing Leaf Spot on Dendrobium officinale in ChinaPeng Cao, Yuhui Fang, Zikui Zheng, Xia Han, Huixi Zou, and Xiufeng Yan11 May 2022 | Plant Disease, Vol. 106, No. 6First Report of Cucumber Fruit Rot Caused by Fusarium incarnatum in MexicoR. S. García-Estrada, I. Márquez-Zequera, J. M. Tovar-Pedraza, and I. Cruz-Lachica8 December 2020 | Plant Disease, Vol. 105, No. 2Effect of no-tillage and tillage systems on melon ( Cucumis melo L.) yield, nutrient uptake and microbial community structures in greenhouse soils17 October 2020 | Folia Horticulturae, Vol. 32, No. 2First report of leaf spot on Cucurbita pepo caused by Fusarium incarnatum‐equiseti species complex in Jamaica21 August 2019 | New Disease Reports, Vol. 40, No. 1

A Gram-stain-positive, aerobic, heterotrophic, non-spore-forming and rod-shaped strain, designated SJ-23(T), was isolated from rhizosphere soil of wheat (Triticum aestivum L.) collected from Langfang, Hebei Province, central PR China and characterized using a polyphasic approach. Morphological and chemotaxonomic characteristics were consistent with those of members of the genus Agromyces. The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, glycolipid and three unidentified lipids. The predominant menaquinones detected were MK-12, MK-11 and MK-10. Major fatty acids were identified as anteiso-C-17 : 0, anteiso-C-15 : 0 and iso-C-16 : 0. The 16S rRNA gene sequence analysis showed that strain SJ-23(T) belongs to the genus Agromyces with high sequence similarities to Agromyces ramosus DSM 43045(T) (99.2 %), Agromycescerinus subsp. cerinus DSM 8595(T) (98.8 %) and Agromyces cerinus subsp. nitratus DSM 8596(T) (98.6 %). Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain formed a separate branch in the genus Agromyces. Furthermore, the combination of DNA-DNA hybridization results and some phenotypic characteristics demonstrated that strain SJ-23(T) could be distinguished from its closest relatives. Therefore, it is proposed that strain SJ-23(T) represents a novel species of the genus Agromyces, for which the name Agromyces tardus sp. nov. is proposed. The type strain is SJ-23(T) (=CGMCC 4.7419(T)=DSM 105049(T)).

A Gram-stain positive, aerobic, rod-shaped bacterium, designated strain NEAU-85T, was isolated from rhizosphere soil of lettuce and characterised using a polyphasic approach. Strain NEAU-85T was found to be catalase positive, motile and able to grow at between 10 and 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-85T belongs to the genus Cryobacterium and is closely related to the type strain Cryobacterium psychrotolerans JCM 13925T (98.5%) and also to Leifsonia kafniensis NCCB 100216T (97.6%). Multilocus sequence analysis using the concatenated gene sequences of the atpD, dnaK, recA, rpoB, sevY and ychF genes showed that strain NEAU-85T belongs to the genus Cryobacterium. The digital DNA–DNA hybridization values revealed that strain NEAU-85T is distinct from its close phylogenetic neighbour C. psychrotolerans. The menaquinones were identified as MK-10 and MK-11. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phospholipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as anteiso-C15:0, anteiso-C15:1 and iso-C16:0. The genomic DNA G + C content of strain NEAU-85T was determined to be 68.9 mol%. The DNA–DNA hybridization value between them was less than 70%. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-85T can be concluded to represent a novel species of the genus Cryobacterium, for which the name Cryobacterium tepidiphilum sp. nov. is proposed. The type strain is NEAU-85T (= CCTCC AA 2018035T = JCM 32545T).

A novel actinobacterium, designated strain NEAU-YG30T, was isolated from the rhizosphere soil of lettuce collected in Heilongjiang province, north-east China. The taxonomic position of the strain was investigated using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain NEAU-YG30T belongs to the genus Nonomuraea, and shared highest sequence similarity to Nonomuraea muscovyensis KCTC 29233T (97.9 %) and Nonomuraea indica CCTCC AA 209050T (97.6 %). The major menaquinone was identified as MK-9(H4). The major fatty acids were 10-methyl C17 : 0, C17 : 0, iso-C16 : 0 2-OH, iso-C16 : 0 and C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, phosphatidylinositol, an unidentified phospholipid and three unidentified lipids. The genomic DNA G+C content of strain NEAU-YG30T was 70.5 mol%. DNA-DNA hybridization values between them were less than 70 %. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-YG30T can be characterized to represent a novel species of the genus Nonomuraea, for which the name Nonomuraealactucae sp. nov. is proposed. The type strain is NEAU-YG30T (=JCM 32549T=CGMCC 4.7506T).

A novel actinomycete, designated strain 1H-SSA4(T), was isolated from the head of an ant (Camponotus japonicus Mayr) and was found to produce angucyclinone antibiotics. A polyphasic approach was used to determine the taxonomic status of strain 1H-SSA4(T). The DNA G+C content of the draft genome sequence, consisting of 11.4 Mbp, was 70.0 mol%. 16S rRNA gene sequence similarity studies showed that strain 1H-SSA4(T) belongs to the genus Streptomyces with the highest sequence similarity to Streptomyces hygroscopicus subsp. ossamyceticus NBRC 13983(T) (98.9 %), and phylogenetically clustered with this species, Streptomyces torulosus LMG 20305(T) (98.8 %), Streptomyces ipomoeae NBRC 13050(T) (98.5 %) and Streptomyces decoyicus NRRL 2666(T) (98.4 %). The morphological and chemotaxonomic properties of the strain were also consistent with those members of the genus Streptomyces. A combination of DNA-DNA hybridization experiments and phenotypic tests were carried out between strain 1H-SSA4(T) and the above-mentioned strains, which further clarified their relatedness and demonstrated that strain 1H-SSA4(T) could be distinguished from these strains. Therefore, the strain is concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces capitiformicae sp. nov. is proposed. The type strain is 1H-SSA4(T) (=CGMCC 4.7403(T) = DSM 104537(T)).