BACKGROUND:The role of electrochemotherapy (ECT) using intratumoral bleomycin and electroporation as a first line treatment for oral tongue carcinoma has not been defined. AIMS/OBJECTIVES:To evaluate the method of ECT in oral tongue carcinoma. MATERIAL AND METHODS:Twenty-one successive patients with primary T1-T2 oral cancer predominantly of the oral tongue underwent either ECT (test; n = 9), or standard surgical resection and reconstruction (control; n = 12). Outcome variables were: local recurrence rates, 10-year-survival, adverse events, treatment cost, and quality of life. RESULTS:The 10-year local recurrence rate (44.4%) was higher and the tumour-specific survival rate (55.6%) was lower in the ECT group compared to the control group (17% and 91.6%, respectively). Postoperative haemorrhage, dysphagia, and pain were more frequent in ECT patients, treatment time was shorter, but treatment cost was higher. Quality of life was not improved by ECT. CONCLUSIONS AND SIGNIFICANCE:Our results indicate that ECT seems not as suitable for the treatment of early tongue cancer as it is for neoplastic and metastatic skin lesions and less favourable than standard surgical therapy.

Background: Porcine mesenchymal stromal cells (pMSCs) are considered a valuable research model for bone tissue engineering, which requires adequate amounts of viable cells with sufficient potential for osteogenic differentiation. For isolation and expansion of these cells through long-term culture, appropriate culture conditions are needed. Objective: To study the effect of extended in vitro cultivation on pMSC proliferation and differentiation potential using different osteogenic and adipogenic induction media. Methods: pMSCs were isolated from the bone marrow of adult Göttingen minipigs, cultured, expanded to passage 20 (~160 days) and characterized by their expression of cell surface markers (wCD44, CD45, CD90, SWC9, fibronectin), alkaline phosphatase (ALP), and osteocalcin and their potential for osteogenic and adipogenic differentiation using different induction media. Results: pMSCs retained their capacity for proliferation and osteogenic differentiation, and the number of CD90-positive cells increased significantly over more than 60 population doublings. CD90 expression in uninduced cells correlated strongly with ALP expression following osteogenic induction. Medium enriched with calcium yielded a stronger osteogenic response. Conclusion: The selection of CD90-positive MSCs and adequate levels of calcium seem to enhance the osteogenic phenotype for bone tissue engineering.

Fig. 1.Patients 1 (IV.2) (A), 2 (IV.3) (B) and 3 (IV.7)(C) with numerous (recurrent) 1-2 mm clear or bluish cystic lesions of translucent or milky opacity bilaterally on the upper and lower eyelid margins.

Introduction: Secondary rhinoplasty in cleft lip and palate (CLP) is commonly the last step in a set of surgical procedures that result in a variable but typically intensive change in facial appearance. However, there is evidence that the sentiment about the aesthetic and functional outcomes between patients and surgeons is different. The present study aimed to evaluate the subjective and objective outcomes of secondary rhinoplasty in patients with CLP.Material and methods: Secondary rhinoplasty was performed in 10 patients with repaired unilateral CLP via a standardized open approach. For the subjective evaluation, the patients completed the rhinoplasty outcome evaluation (ROE) questionnaire. Pre- and postoperative photographic documentation served as the basis for the objective evaluation, which included the following: (1) assessment by five specialists at craniofacial malformation consultation appointments and by three doctors in continuing education using the Asher-McDade aesthetic index (AMAI) rating, and (2) metric facial analysis to determine the nasofrontal angle and the nasolabial angle.Results: Patient satisfaction was high, based on the evaluation of the ROE questionnaire. The analysis of the AMAI rating questionnaire showed no significant differences between the positive ratings of the 'experienced' and 'inexperienced' doctors. In contrast, there was an obvious and significant difference between the 'preoperative' and 'postoperative' time points for questions 1-3. The metric analysis showed statistically significant improvements of the nasolabial angle and the nasofrontal angle. The subjective and objective outcome evaluations were descriptively congruent.Conclusions: The data suggest that standardized secondary rhinoplasty in CLP leads to both a subjective and a statistically significant objective improvement of facial appearance and thus may support the psychosocial rehabilitation of affected patients. Furthermore, our results showed that the subjective and objective outcome evaluations of secondary rhinoplasty were largely compatible. (C) 2015 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.

This study was designed to trace bone marrow-derived stromal cells (MSC) after implantation in an ectopic rat model of bone tissue engineering. MSC were isolated from adult donor rats, expanded, seeded on a hydroxyapatite/β-tricalcium phosphate bone graft substitute (Straumann® BoneCeramic), and cultivated until confluent. Before subcutaneous implantation of seeded constructs and controls (unseeded bone graft substitute) in isogenic rats (n = 32), cells were labeled with the fluorescent dye carboxyfluoresceine-diacetate-succinimidyl-ester. Specimens were harvested at sacrifice on day 1, 3, 7, or 14 after implantation (n = 8 per group) and processed for histology (hematoxylin and eosin, CD68, 4',6-diamidino-2-phenylindol). Carboxyfluoresceine-diacetate-succinimidyl-ester-labeled transplanted cells were quantified in decalcified sections (50 fields of view per specimen) at 488 nm. Over time, transplanted cells decreased in number from 31.3 ± 2.3 (day 1) to 9.2 ± 1.1 (day 3) and 0.3 ± 0.1 (day 7) (p < 0.001). Fourteen days postimplantation MSC could no longer be identified. Additionally, starting on day 3 postimplantation, cellular disintegration was noted. Multinucleated giant cells were present in constructs and controls on day 7 and increased to day 14 postimplantation. These results indicate that ectopically transplanted MSC survive for a rather short time after implantation. Possible reasons for early cell death are discussed.

Bone graft substitutes (BGS) are widely used in clinical practice. For stem cellbased approaches to bone tissue engineering BGS need to show sufficient biocompatibility in the in vitro setting. This study was designed to demonstrate the influence of six different BGS on the proliferation and metabolic activity of porcine mesenchymal multilineage stem cells (pMSC) in vitro. Bone-marrow derived pMSC were cultivated for 24 hours with the eluates of six different BGS. The eluates were generated by incubating the BGS three times in succession for 24 hours with a culture medium and collecting the supernatants. pMSC vitality and proliferation in the presence of eluates from the first, second, and third incubation were assessed by WST-test quantification of metabolically active cells. Culture of pMSC with eluates in all cases resulted in decreased cell numbers in an eluate concentration-dependent manner. At least a 65% loss of cells compared to controls (culture medium without eluates) could be observed in the presence of undiluted eluates. The negative influence of eluates varied significantly among BGS. In all cases, second and third eluates were less potent in their negative effects on cellular vitality/proliferation. In conclusion, the BGS examined here should be submitted to thorough preincubation before in vitro use for cell-based constructs to maximize cell viability for the tissue engineering of bone.

OBJECTIVES With natural bovine bone mineral (BioOss) as carrier, the study aimed at investigating the effect of autogenous osteoblast-like cells on bone regeneration in an orthotopic (maxillary sinus) and an ectopic (muscle) site. MATERIALS AND METHODS Autogenous osteoblast-like cells were isolated from iliac cancellous bone of six minipigs and expanded in an autogenous serum-supplemented osteogenic medium. After confirmation of osteogenicity, the expanded cells were precultivated on BioOss granules for 1 week. Four milliliter of cell-seeded BioOss were used for sinus augmentation of right sinus and a Straumann solid screw (dental) implant was simultaneously installed. The contralateral (control) side was filled with cell-free BioOss. Besides, 2 ml of the corresponding granules were placed in a pouch in the latissimus dorsi muscles bilaterally. Polychrome sequential labeling was performed postoperatively. Specimens were harvested at week 6. Undecalcified sections were evaluated with microradiography, fluorescence microscopy, histology and histomorphometry. RESULTS In the test side, the coronal part of dental implant demonstrated higher bone-implant contact (BIC) than the apical part (34.88+/-28.86% vs. 16.68+/-13.80%, P=0.039), as well as higher bone density (BD) in the corresponding zone (14.88+/-6.37% vs. 11.10+/-4.54%, P=0.021). However, the test side demonstrated no advantage over control side in either BD (12.25+/-4.22% vs. 8.45+/-11.04%, P=0.473) or BIC (24.15+/-21.97% vs. 22.05+/-19.00%, P=0.270). Rare bone was formed in the muscles in both sides. CONCLUSIONS The expanded autogenous osteoblast-like cells failed to enhance bone formation in the minipig model of sinus augmentation.

Wir berichten über die ungewöhnliche Manifestation und Therapie eines Dermatofibrosarcoma protuberans im Gesicht.

We here report on the unusual manifestation and management of a Dermatofibrosarcoma protuberans in the face.

AIM:The aim of the present study was to evaluate the osseointegration of dental implants and bone formation in maxillary sinus grafting with autologous and anorganic bovine bone in the presence of platelet-rich plasma (PRP) in an established animal model.MATERIAL AND METHODS:We performed bilateral maxillary sinus augmentation with 50% anorganic bovine bone and simultaneously inserted a titanium screw implant in five minature pigs. Six hundred microlitre autologous PRP were added to the left side (test). The right side (no PRP) served as control. Polychrome sequential labeling was performed. The animals were sacrificed 6 weeks after surgery. Undecalcified ground sections were evaluated by microradiography, digitized histomorphometry and under fluorescent light.RESULTS:The mean bone implant content in the test and control group was 8.4% and 17.3% respectively (P=0.042). The mean height of newly formed mineralized bone in the augmented area of the test group was 3.6 mm and 5.7 mm respectively (P=0.342). In the PRP group, the mean area of newly formed bone in the base of the sinus was enhanced (75.23%) as compared to the control side (51.8%) (P=0.020(*)). Although PRP enhanced bone formation at the base of the maxillary sinus, it neither improved osseointegration of dental implants nor bone in-growth into the bone substitute under the selected experimental conditions.

The development of an invasive squamous cell carcinoma within a lateral cervical cyst as a result of malignant transformation of the epithelium is considered a rare circumstance. The existence of this entity is a source of controversy in light of the differential diagnosis, which includes a cervical metastasis from an unknown primary carcinoma (CUP‐syndrome). Apart from site, histologic findings, and follow‐up, the principal diagnostic criterion for lateral cervical cyst carcinoma is the histomorphologic demonstration of transition of the benign epithelium into invasive squamous cell carcinoma. Although numerous case reports of this entity exist, carcinoma in situ in a lateral cervical cyst has been reported in only five cases thus far.

. The free microvasclar fibula and soft tissue transfer has become a widely used method to reconstruct the head and neck region. Only few reports focus on the donor site morbidity of purely mandibular reconstructions. On the basis of the hospital charts, a standardized patient interview and a standardized physical examination, the present study evaluates the early and late donor site morbidity in 42 mostly oncologic patients after an average follow-up interval of 34 months. Of these, 16 patients (38.1%) exhibited complicated wound healing that did not correlate with the patient's age, sex, type of transplant, use of a skin graft, result of preoperative angiography, rate of postoperative mobilization or the incidence of late donor site morbidity. At the time of follow-up examination, about one-fourth of the patients reported discomfort, pain or swelling. Objective findings included sensory deficits in 76.3%, motor deficits in 39.5% and reduced strength in 44.7% of the patients. Even though objectively measured morbidity exceeded subjectively perceived morbidity, it can be concluded that there is significant early and late donor site morbidity, which should be considered when opting for a fibula graft. Patients should be informed accordingly.

The anatomical region "laterodorsal calf" with the fibula, the flexor muscles and the overlying skin served by the peroneal vessels, is a suitable donor region particularly for combined microsurgical transfer of bone and soft tissue. In a five and a half year period, 59 reconstructions, 39 (66%) with and 20 (34%) without soft tissue transfer, were performed. The versatility and the functional and esthetic results after osseous, osteocutaneous, osteomuscular and osteomyocutaneous tissue transfer are presented.

Crop ScienceVolume 36, Issue 5 cropsci1996.0011183X003600050069x p. 1416-1416 Registration of Cultivars Registration of ‘Aquila’ Hop R. R. Romanko, Corresponding Author R. R. Romanko [email protected] Irrigated Agric. Res. and Ext. Ctr., Washington State Univ., Prosser, WA, 99350Corresponding author ([email protected]).Search for more papers by this authorG. B. Nickerson, G. B. Nickerson Dep. of Agric. Chemistry, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorJ. Jaeger, J. Jaeger Hermiston Agric. Res. and Ext. Ctr., Oregon State Univ., Hermiston, OR, 97838Search for more papers by this authorS. T. Kenny, S. T. Kenny Parma Res. and Ext. Ctr., Univ. of Idaho, Parma, ID, 83660Search for more papers by this authorC. B. Skotland, C. B. Skotland Parma Res. and Ext. Ctr., Univ. of Idaho, Parma, ID, 83660Search for more papers by this author R. R. Romanko, Corresponding Author R. R. Romanko [email protected] Irrigated Agric. Res. and Ext. Ctr., Washington State Univ., Prosser, WA, 99350Corresponding author ([email protected]).Search for more papers by this authorG. B. Nickerson, G. B. Nickerson Dep. of Agric. Chemistry, Oregon State Univ., Corvallis, OR, 97331Search for more papers by this authorJ. Jaeger, J. Jaeger Hermiston Agric. Res. and Ext. Ctr., Oregon State Univ., Hermiston, OR, 97838Search for more papers by this authorS. T. Kenny, S. T. Kenny Parma Res. and Ext. Ctr., Univ. of Idaho, Parma, ID, 83660Search for more papers by this authorC. B. Skotland, C. B. Skotland Parma Res. and Ext. Ctr., Univ. of Idaho, Parma, ID, 83660Search for more papers by this author First published: 01 September 1996 https://doi.org/10.2135/cropsci1996.0011183X003600050069xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article. Volume36, Issue5September–October 1996Pages 1416-1416 RelatedInformation

Crop ScienceVolume 26, Issue 1 cropsci1986.0011183X002600010049x p. 196-197 Registration of Crop Cultivars Registration of ‘Chinook’ Hop S. T. Kenny, S. T. KennySearch for more papers by this authorC. E. Zimmermann, C. E. ZimmermannSearch for more papers by this author S. T. Kenny, S. T. KennySearch for more papers by this authorC. E. Zimmermann, C. E. ZimmermannSearch for more papers by this author First published: 01 January 1986 https://doi.org/10.2135/cropsci1986.0011183X002600010049xCitations: 8AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume26, Issue1January–February 1986Pages 196-197 RelatedInformation

plants having long, narrow, drooping leaves. Stems are straight-necked and yellow at maturity. Distance from the flag leaf to the spike ranges from 14 to 22 cm; the collar is closed and the basal internode is straight and 1 to 3 mm long. The rachis is tough with glabrous edges. Kline is resistant to spot blotch, caused by Cochliobolus sativus (Ito & Kurib.) Drechs. ex Dast., and barley yellow dwarf virus (BYDV). It is moderately resistant to leaf rust and scald, caused by Rhynchosporium secalis (Oud.) JJ. Davis. Kline is moderately susceptible to septoria glume blotch, caused by Leptosphaeria nodorum Muller, and is susceptible to powdery mildew and loose smut, caused by Ustilago nuda (Jens.) Rostr. Powdery mildew is not a serious disease in the Southeast and loose smut can be controlled by carboxin (Vitavax) seed treatment. This cultivar was named to honor the late Dr. David Kline, USDA-ARS, cereal pathologist, North Carolina State Univ., Raleigh. Dr. Kline pioneered in research on new fungicides to control small grain diseases after organic mercurial compounds were banned early in the 1970s. Breeder seed will be maintained by the Agronomy Dep., College Exp. Stn., Athens, GA 30602. Foundation seed will be maintained by the Georgia Seed Development Commission, 2420 South Milledge Avenue, Athens, GA 30605.

U.S. government support for hop research started at Oregon State University in 1930 when most American hops were grown in that state. Research was aimed at finding genetic resistance to downy mildew by breeding and germ plasm introduction and at developing chemical control measures against the disease. Later, work expanded to include agronomic, physiological, and chemical investigations. U.S. Department of Agriculture scientists independently, and in cooperation with state scientists in Idaho and Washington, released several new hop varieties that now account for about 35% of total U.S. hop production. Significant contributions to hop growing and utilization also came from research in hop pathology, chemistry, agronomy, and physiology.KeywordsBreedingGerm plasmHop diseasesPhysiologyVarieties

Crop ScienceVolume 24, Issue 3 cropsci1984.0011183X002400030046x p. 618-619 Registration of Crop Cultivars Registration of Olympic Hop S. T. Kenny, S. T. Kenny Research associate Irrigated Agric. Res. and Ext. Ctr., Washington State Univ., Prosser, WA, 99350Search for more papers by this authorC. E. Zimmermann, C. E. Zimmermann formerly plant pathologist, vice-president USDA-ARS, Irrigated Agric. Res. and Res. and Ext. Ctr., Prosser, WA, 99350Search for more papers by this author S. T. Kenny, S. T. Kenny Research associate Irrigated Agric. Res. and Ext. Ctr., Washington State Univ., Prosser, WA, 99350Search for more papers by this authorC. E. Zimmermann, C. E. Zimmermann formerly plant pathologist, vice-president USDA-ARS, Irrigated Agric. Res. and Res. and Ext. Ctr., Prosser, WA, 99350Search for more papers by this author First published: 01 May 1984 https://doi.org/10.2135/cropsci1984.0011183X002400030046xCitations: 15AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume24, Issue3May–June 1984Pages 618-619 RelatedInformation

The sum of (α+β) acids in lupulin glands of 112 female and 24 male genotypes of hop (Humulus lupulus L.) averaged 73 ± 6% by weight. Alpha‐ and beta‐acid content in glands from male or female hops was inversely correlated (r = −0.78 for females; −0.85 for males). A significant change in the a‐acid content of lupulin was associated with a similar change of β‐acid content in the opposite direction.The α‐acid content of hop cones was related to the quantity and quality of lupulin in the cones. The lupulin quantity was proportional to the sum of (α+β). Lupulin quality was related to the ratio of α‐ and β‐acids (α/β).Six test crosses were made with parents having high or low α/β values, respectively. The average α/β value for progenies from each cross was nearly identical to the parental midpoint. Data presented in this paper led to formulation of a new working hypothesis for the relationship between hop acids and lupulin content. Lupulin analysis is simple, and a modest laboratory can screen several hundred genotypes each season.

Crop ScienceVolume 18, Issue 5 cropsci1978.0011183X001800050085x p. 919-919 Registration of Germplasms Registration of USDA 21055 Hop Germplasm1 (Reg. No. GP 5) Alfred Hounold, Alfred HounoldSearch for more papers by this authorS. T. Likens, S. T. LikensSearch for more papers by this authorG. B. Nickerson, G. B. NickersonSearch for more papers by this authorC. E. Horner, C. E. HornerSearch for more papers by this authorC. E. Zimmermann, C. E. Zimmermann Research geneticist and chemist, respectively, FR, SEA, USDA; chemist, Dep. of Agricultural Chemistry, Oregon State Univ.; research plant pathologist, FR-SEA-USDA, Corvallis, OR 97331; and research plant physiologist, FR-SEA-USDA, Prosser, WA 99350.Search for more papers by this author Alfred Hounold, Alfred HounoldSearch for more papers by this authorS. T. Likens, S. T. LikensSearch for more papers by this authorG. B. Nickerson, G. B. NickersonSearch for more papers by this authorC. E. Horner, C. E. HornerSearch for more papers by this authorC. E. Zimmermann, C. E. Zimmermann Research geneticist and chemist, respectively, FR, SEA, USDA; chemist, Dep. of Agricultural Chemistry, Oregon State Univ.; research plant pathologist, FR-SEA-USDA, Corvallis, OR 97331; and research plant physiologist, FR-SEA-USDA, Prosser, WA 99350.Search for more papers by this author First published: 01 September 1978 https://doi.org/10.2135/cropsci1978.0011183X001800050085x 1 Registered by the Crop Sci. Soc. Am. Contribution of FR-SEA-USDA in cooperation with the U.S. Brewers Association and the Washington and Oregon Agric. Exp. Stns. Technical Paper No. 4772. Accepted AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article. Volume18, Issue5September–October 1978Pages 919-919 RelatedInformation