There exists an extensive, diverse, and robust evidence base to support complex decisions that address the planetary biodiversity crisis. However, it is generally not sought or used by environmental decision-makers, who instead draw on intuition, experience, or opinion to inform important decisions. Thus, there is a need to examine evidence exchange processes in wildlife management to understand the multiple inputs to decisions. Here, we adopt a novel approach, fuzzy cognitive mapping (FCM), to examine perceptions of individuals from Indigenous and Western governments on the reliability of evidence which may influence freshwater fisheries management decisions in British Columbia, Canada. We facilitated four FCM workshops participants representing Indigenous or Western regulatory/governance groups of fisheries managers. Our results show that flows of evidence to decision-makers occur within a relatively closed governance network, constrained to the few well-connected decision-making organizations (i.e., wildlife management agencies) and their close partners. This implies that increased collaboration (i.e., knowledge co-production) and engagement (i.e., knowledge brokerage) with wildlife managers and decision-makers are needed to produce actionable evidence and increase evidence exchange.

Recalling its decision 14/34, in which it adopted the preparatory process for the development of the Kunming-Montreal global biodiversity framework and decided to establish an open-ended intersessional working group to support its preparation, Noting also the outcomes of the first, 1 second, 2 third 3 fourth 4 and fifth meetings 5 of the Open-ended Working Group on the Post-2020 Global Biodiversity Framework, of the regional and thematic consultations and workshops conducted based on decision 14/34 and of the intersessional work conducted on digital sequence information on genetic resources, Noting the outcomes of the eleventh meeting of the Ad Hoc Open-ended Working Group on Article 8(j) and Related Provisions, of the twenty-third and twenty-fourth meetings of the Subsidiary Body on Scientific, Technical and Technological Advice and of the third meeting of the Subsidiary Body on Implementation, Expressing its gratitude to the following Governments of […..] for hosting these consultations, as well as for their financial contributions, Expressing its gratitude to the Co-Chairs of the Open-ended Working Group on the Post-2020 Global Biodiversity Framework, Mr. Basile van Havre (Canada) and Mr. Francis Ogwal (Uganda), for supporting the development of the Kunming-Montreal global biodiversity framework, Welcoming the submissions by Parties, other Governments, indigenous peoples and local communities, United Nations organizations and programmes, other multilateral environmental agreements, subnational governments, cities and other local authorities, intergovernmental organizations, nongovernmental organizations, women's groups, youth groups, the business and finance community, the scientific community, academia, faith-based organizations, representatives of sectors related to or 1 CBD/WG2020/1/5. 2CBD/WG2020/2/4. 3CBD/WG2020/3/5.

A critical unmet need for advanced prostate cancer (PCa) patients is optimizing systemic treatments to maximize the benefit for individuals. The response of patients with metastatic castration-resistant prostate cancer (mCRPC) to androgen receptor (AR)-directed hormonal treatments (i.e., enzalutamide and abiraterone) is mediated by the expression of a molecular variant of the androgen receptor called androgen receptor variant 7 (AR-V7). Detection and measurement of AR-V7 in mCRPC patients will lead to more informed PCa treatment. Herein, we demonstrate a quantitative nanoparticle-enhanced sandwich antibody assay for the successful ex vivo measurement of AR-V7 protein in serum from mCRPC patients. The nanoparticles are constructed as extrinsic Raman spectroscopy labels (ERLs), and surface-enhanced Raman spectroscopy (SERS) is used for assay readout. Our approach does not require specialized specimen collection materials, circulating tumor cell enrichment, or pretreatment of serum. Calibration of our assay is accomplished by expressing AR-V7 in an appropriate cell line as AR-V7 is not commercially available. We demonstrate a linear calibration curve from cell lysate and correlate lysate protein with mRNA from cultured prostate cancer cells. Finally, we demonstrate a novel pilot-scale application for clinical use by quantitatively measuring AR-V7 in serum of seven advanced PCa patients. Distinct separation of PCa patients by AR-V7 status (positive or negative) was observed. Together, the presence and amount of AR-V7 in serum offer predictive and prognostic value to inform selection between two classes of systemic treatments (i.e., hormones or taxanes). Triaging patients that are AR-V7-positive to other systemic treatments (e.g., taxane-based chemotherapy) can improve progression-free survival and overall survival.

The economic valuation of ecosystem services in part reflects the desire to use conventional economic tools (markets and economic instruments) to conserve ecosystem services. However, for regulating and supporting ecosystem services that depend on ecosystem structure and function, estimation of economic value requires estimates of the current level of underlying ecological functions first. This primary step is in principle, the job of environmental scientists, not economists. Here, we provide a coarse-level quantitative assessment of the relationship between the research effort expended by environmental scientists (on the biophysical values) and economists (on the monetary values) on 15 different regulating and supporting services in 32 ecosystem types using peer-reviewed article hits retrieved from bibliographic databases as a measure of research effort. We find a positive, moderately strong (r = 0.69) correlation between research efforts in the two domains, a result that, while encouraging, is likely to reflect serendipity rather than the deliberate design of integrated environmental science-economics research programs. Our results suggest that compared to environmental science research effort economic valuation is devoted to a smaller, less diverse set of ecosystem services but a broader, more diverse, set of ecosystem types. The two domains differed more with respect to the ecosystem services that are the major focus of research effort than they did with respect to the ecosystem types of principal research interest. For example, carbon sequestration, erosion regulation, and nutrient cycling receive more relative research effort in the environmental sciences; air quality regulation in economic valuations. For both domains, cultivated areas, wetlands, and urban/semi-urban ecosystem types received relatively large research effort, while arctic and mountain tundra, cave and subterranean, cryosphere, intertidal/littoral zone, and kelp forest ecosystem types received negligible research effort. We suggest ways and means by which the field of sustainability science may be improved by the design and implementation of a searchable database of environmental science and economic valuation literature as well as a global ecosystem service research network and repository that explicitly links research on the estimation and prediction of biophysical ecosystem functions with that of the social sciences and other knowledge systems. These suggestions would, at least in principle, facilitate a more efficient research agenda between economists and environmental scientists and aid management, regulatory and judicial decision-makers.

Although agricultural “best (or beneficial) management practices” (BMPs) first emerged to mitigate agro-environmental resource challenges, they may also enhance ‘non-provisioning’ ecosystem services. The enthusiasm for adopting BMPs partially depends on evidence that doing so will lead to agro-environmental benefits while not substantially reducing crop productivity or farmer income. We survey and synthesize evidence in the existing literature to document the joint effects on agricultural crop yield and 12 ecosystem service (ES) associated with implementation of 5 agricultural BMPs (crop rotations, cover crops, nutrient management, perennial vegetated buffers, reduced or no tillage). We also analyze the prevalence of co-benefits (‘win-win’), tradeoffs, and co-costs (‘lose-lose’) outcomes. On the basis of a set of contextual variables we then develop empirical models that predict the likelihood of co-benefits relative to tradeoffs, and co-costs. We found thirty-six studies investigating 141 combinations of crop yields and non-provisioning ES outcomes (YESs) in the relevant literatures covering the period 1983-2016. The scope of the review is global, but included studies are geographically concentrated in the U.S. Corn Belt (Midwestern United States). In the literature sample, reporting of co-benefits (26%) was much more prevalent than reporting of co-costs (4%) between yields and ES. Tradeoffs most often resulted in a reduction in crop yields and an increase in ES (28%); this was marginally greater than studies reporting a neutral influence on crop yields and an increase in ES (26%). Other Y/ES combinations were uncommon. Mixed-effects models indicated reduced tillage and crop rotations had generally positive associations with YESs. Temporal scale was an informative predictor suggesting studies with longer time scales resulted in greater positive outcomes on YESs, on average. Our results are a step towards identifying those contexts where co-benefits or partial improvement outcomes of BMPs are more likely to be realized, as well as the impact of particular practices on specific ES.

Effects of species diversity on population and community stability (or more precisely, the effects of species richness on temporal variability) have been studied for several decades, but there have been no large-scale tests in natural communities of predictions from theory. We used 91 data sets including plants, fish, small mammals, zooplankton, birds, and insects, to examine the relationship between species richness and temporal variability in populations and communities. Seventy-eight of 91 data sets showed a negative relationship between species richness and population variability; 46 of these relationships were statistically significant. Only five of the 13 positive richness-population variability relationships were statistically significant. Similarly, 51 of 91 data sets showed a negative relationship between species richness and community variability; of these, 26 were statistically significant. Seven of the 40 positive richness-community-variability relationships were statistically significant. We were able to test transferability (i.e., the predictive ability of models for sites that are spatially distinct from sites that were used to build the models) for 69 of 91 data sets; 35 and 31 data sets were transferable at the population and community levels, respectively. Only four were positive at the population level, and two at the community level. We conclude that there is compelling evidence of a negative relationship between species richness and temporal variability for about one-half of the ecological communities we examined. However, species richness explained relatively little of the variability in population or community abundances and resulted in small improvements in predictive ability.

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Wetlands potentially provide a range of ecological (or ecosystem) services including ground water recharge, nutrient retention, waste assimilation, shoreline stabilization, and carbon storage. One of the most cited and valuable services potentially provided by wetlands are their influence on flow regimes, especially flood attenuation and augmentation of low flows. Here we report the results of a meta-analysis of twenty-eight studies, including fifty-nine associated effect sizes, that have investigated the flow regulation services of wetlands. We found that, consistent with conventional wisdom, on average wetlands reduce the frequency and magnitude of floods and increase flood return period; augment low flows; and decrease runoff and streamflow. However, our results also indicate gross wetland characteristics have little predictive power with respect to the observed variation in the level of flow regulation services. This implies that in that in the absence of detailed site-specific information, estimates of flow regulation services provided by wetlands will generally have large uncertainty, as will any associated estimate of their economic value.

Road traffic kills hundreds of millions of animals every year, posing a critical threat to the populations of many species. To address this problem there are more than forty types of road mitigation measures available that aim to reduce wildlife mortality on roads (road-kill). For road planners, deciding on what mitigation method to use has been problematic because there is little good information about the relative effectiveness of these measures in reducing road-kill, and the costs of these measures vary greatly. We conducted a meta-analysis using data from 50 studies that quantified the relationship between road-kill and a mitigation measure designed to reduce road-kill. Overall, mitigation measures reduce road-kill by 40% compared to controls. Fences, with or without crossing structures, reduce road-kill by 54%. We found no detectable effect on road-kill of crossing structures without fencing. We found that comparatively expensive mitigation measures reduce large mammal road-kill much more than inexpensive measures. For example, the combination of fencing and crossing structures led to an 83% reduction in road-kill of large mammals, compared to a 57% reduction for animal detection systems, and only a 1% for wildlife reflectors. We suggest that inexpensive measures such as reflectors should not be used until and unless their effectiveness is tested using a high-quality experimental approach. Our meta-analysis also highlights the fact that there are insufficient data to answer many of the most pressing questions that road planners ask about the effectiveness of road mitigation measures, such as whether other less common mitigation measures (e.g., measures to reduce traffic volume and/or speed) reduce road mortality, or to what extent the attributes of crossing structures and fences influence their effectiveness. To improve evaluations of mitigation effectiveness, studies should incorporate data collection before the mitigation is applied, and we recommend a minimum study duration of four years for Before-After, and a minimum of either four years or four sites for Before-After-Control-Impact designs.

The idea that there is a relationship between our well-being and the well-being of the natural world has been explored by philosophers and scientists of all stripes through the ages. So the basic t...

Protected area legislation provides the statutory authority for the establishment and management of protected areas. Yet few studies have investigated the relationship between protected area legislation and those attributes of protected areas that are likely to affect their success in achieving biodiversity conservation objectives. Here we investigate the association between the size and number of protected areas within Canadian provincial, territorial and federal jurisdictions and provisions of the corresponding legislation using a Before–After/Control–Impact design. We found that jurisdictions with legislation that includes explicit provisions for donations in cash or in-kind and many types of stakeholder involvement had, on average, larger (1.01× to 29.0×) protected areas after versus before legislation enactment, compared to those without such provisions. Jurisdictions with legislation that includes provisions for protected area co-management with local or aboriginal/indigenous communities also had, on average, a higher rate of park establishment after (0.17–23.7 protected areas/year) versus before (0.17–6.34 protected areas/year) legislation enactment, compared to those without such provisions (0.09–5.00 protected areas/year; 0.21–5.30 protected areas/year after and before respectively). Similar patterns were detected for jurisdictions with legislation that includes provisions for operating and/or capital cost recovery. Our results suggest that legislative provisions that facilitate stakeholder participation and cost recovery may contribute to the establishment of more and larger protected areas. As signatories to the Convention on Biological Diversity attempt to expand protected area networks, they should consider including provisions concerning stakeholder involvement and cost recovery into protected areas legislation.

An experimental approach to road mitigation that maximizes inferential power is essential to ensure that mitigation is both ecologically-effective and cost-effective. Here, we set out the need for and standards of using an experimental approach to road mitigation, in order to improve knowledge of the influence of mitigation measures on wildlife populations. We point out two key areas that need to be considered when conducting mitigation experiments. First, researchers need to get involved at the earliest stage of the road or mitigation project to ensure the necessary planning and funds are available for conducting a high quality experiment. Second, experimentation will generate new knowledge about the parameters that influence mitigation effectiveness, which ultimately allows better prediction for future road mitigation projects. We identify seven key questions about mitigation structures (i.e., wildlife crossing structures and fencing) that remain largely or entirely unanswered at the population-level: (1) Does a given crossing structure work? What type and size of crossing structures should we use? (2) How many crossing structures should we build? (3) Is it more effective to install a small number of large-sized crossing structures or a large number of small-sized crossing structures? (4) How much barrier fencing is needed for a given length of road? (5) Do we need funnel fencing to lead animals to crossing structures, and how long does such fencing have to be? (6) How should we manage/manipulate the environment in the area around the crossing structures and fencing? (7) Where should we place crossing structures and barrier fencing? We provide experimental approaches to answering each of them using example Before-After-Control-Impact (BACI) study designs for two stages in the road/mitigation project where researchers may become involved: (1) at the beginning of a road/mitigation project, and (2) after the mitigation has been constructed; highlighting real case studies when available.

Oncolytic viruses (OVs) have shown promising clinical activity when administered by direct intratumoral injection. However, natural barriers in the blood, including antibodies and complement, are likely to limit the ability to repeatedly administer OVs by the intravenous route. We demonstrate here that for a prototype of the clinical vaccinia virus based product Pexa-Vec, the neutralizing activity of antibodies elicited by smallpox vaccination, as well as the anamnestic response in hyperimmune virus treated cancer patients, is strictly dependent on the activation of complement. In immunized rats, complement depletion stabilized vaccinia virus in the blood and led to improved delivery to tumors. Complement depletion also enhanced tumor infection when virus was directly injected into tumors in immunized animals. The feasibility and safety of using a complement inhibitor, CP40, in combination with vaccinia virus was tested in cynomolgus macaques. CP40 pretreatment elicited an average 10-fold increase in infectious titer in the blood early after the infusion and prolonged the time during which infectious virus was detectable in the blood of animals with preexisting immunity. Capitalizing on the complement dependence of antivaccinia antibody with adjunct complement inhibitors may increase the infectious dose of oncolytic vaccinia virus delivered to tumors in virus in immune hosts.

The last 20 years have seen a dramatic increase in efforts to mitigate the negative effects of roads and traffic on wildlife, including fencing to prevent wildlife-vehicle collisions and wildlife crossing structures to facilitate landscape connectivity. While not necessarily explicitly articulated, the fundamental drivers behind road mitigation are human safety, animal welfare, and/or wildlife conservation. Concomitant with the increased effort to mitigate has been a focus on evaluating road mitigation. So far, research has mainly focussed on assessing the use of wildlife crossing structures, demonstrating that a broad range of species use them. However, this research has done little to address the question of the effectiveness of crossing structures, because use of a wildlife crossing structure does not necessarily equate to its effectiveness. The paucity of studies directly examining the effectiveness of crossing structures is exacerbated by the fact that such studies are often poorly designed, which limits the level of inference that can be made. Without well performed evaluations of the effectiveness of road mitigation measures, we may endanger the viability of wildlife populations and inefficiently use financial resources by installing structures that are not as effective as we think they are. In this paper we outline the essential elements of a good experimental design for such assessments and prioritize the parameters to be measured. The framework we propose will facilitate collaboration between road agencies and scientists to undertake research programs that fully evaluate effectiveness of road mitigation measures. We discuss the added value of road mitigation evaluations for policy makers and transportation agencies and provide recommendations on how to incorporate such evaluations in road planning practices.

INTRODUCTION Biology is the study of life, psychology is the study of mind, and medicine is the investigation of the causes and treatments of disease. This chapter describes how the central concepts of life, mind, and disease have undergone fundamental changes in the past 150 years or so. There has been a progression from theological, to qualitative, to mechanistic explanations of the nature of life, mind and disease. This progression has involved both theoretical change, as new theories with greater explanatory power replaced older ones, and emotional change as the new theories brought reorientation of attitudes toward the nature of life, mind, and disease. After a brief comparison of theological, qualitative, and mechanistic explanations, I will describe how shifts from one kind of explanation to another have carried with them dramatic kinds of conceptual change in the key concepts in the life sciences. Three generalizations follow about the nature of conceptual change in the history of science: there has been a shift from conceptualizations in terms of simple properties to ones in terms of complex relations; conceptual change is theory change; and conceptual change is often emotional as well as cognitive. The contention that historical development proceeds in three stages originated with the nineteenth-century French philosophers, Auguste Comte, who claimed that 2 human intellectual development progresses from a theological to a " metaphysical " stage to a " positive " (scientific) stage (Comte, 1988). The stages I have in mind are different from Comte's, so let me say what they involve. By the theological stage I mean systems of thought in which the primary explanatory entities are supernatural ones beyond the concept of fire was initially theological, as in the Greek myth of Prometheus receiving fire from the gods. By the qualitative stage I mean systems of thought that do not invoke supernatural entities, but which postulate natural entities not far removed from what they are supposed to explain, such as vital force in biology. Early qualitative concepts of fire include Aristotle's view of fire as a substance and Epicurus's account of fire atoms. By the mechanistic stage I mean the kinds of developments now rapidly taking place in all of the life sciences in which explanations consist of identifying systems of interacting parts that produce observable changes. The modern concept of fire is mechanistic: combustion is rapid oxidation, the combination of molecules. Much more will be said about the …

In vitro and animal studies report that some persistent organic pollutants (POPs) trigger the secretion of proinflammatory cytokines. Whether POP exposure is associated with a dysregulation of cytokine response remains to be investigated in humans. We studied the strength of association between plasma POP levels and circulating cytokines as immune activation markers. Plasma levels of fourteen POPs and thirteen cytokines were measured in 39 Caucasians from a comparator sample in Québec City (Canada) and 72 First Nations individuals from two northern communities of Ontario (Canada). Caucasians showed significantly higher levels of organochlorine insecticides (β-HCH, p,p'-DDE and HCB) compared to First Nations. Conversely, First Nations showed higher levels of Mirex, Aroclor 1260, PCB 153, PCB 170, PCB 180 and PCB 187 compared to Caucasians. While there was no difference in cytokine levels of IL-4, IL-6, IL-10 and IL-22 between groups, First Nations had significantly greater average levels of IFNγ, IL-1β, IL-2, IL-5, IL-8, IL-12p70, IL-17A, TNFα and TNFβ levels compared to Caucasians. Among candidate predictor variables (age, body mass index, insulin resistance and POP levels), high levels of PCBs were the only predictor accounting for a small but significant effect of observed variance (∼7%) in cytokine levels. Overall, a weak but significant association is detected between persistent organochlorine pollutant exposure and elevated cytokine levels. This finding augments the already existing information that environmental pollution is related to inflammation, a common feature of several metabolic disorders that are known to be especially prevalent in Canada's remote First Nations communities.

Introduced piscivorous fishes had a dramatic impact on small-bodied fish species diversity of small temperate lakes in Gatineau Park, Quebec, Canada, on the basis of three surveys carried out over a 38-year period from 1970 to 2007. For three overlapping sets of lakes based on different combinations of survey years (lakes surveyed in 1970–1971, 1991–1992, and 2006–2007 (N = 14); lakes surveyed in 1970–1971 and 2006–2007 (N = 21); and lakes surveyed in 1991–1992 and 2006–2007 (N = 16)), those with introduced piscivores showed substantial and consistent temporal declines in average minnow species richness but much weaker, if any, declines in total species richness. By contrast, lakes without introduced piscivores showed no such decline. Whereas lakes without piscivores showed a strong species–elevation relationship early in the record, the strength of this relationship was much lower in lakes with introduced piscivores. Moreover, the strength of the species–elevation relationship declined precipitously over time in lakes with introduced piscivores, but remained stable in lakes where introduced piscivores were absent. The negative impact of piscivore introductions on small-bodied fish biodiversity in small lakes underscores the importance of action to mitigate the risk of future introductions or invasions.