Angewandte Chemie International EditionVolume 49, Issue 50 p. 9773-9776 Communication Surface-Bound Microenclosures for Biomolecules† Laiyi Lin, Laiyi Lin Physics Department and NanoCore, National University of Singapore, Singapore 117542 (Singapore), Fax: (+65) 6872-3069 http://www.nanocore.nus.edu.sg/daniel_lubrich.htmlSearch for more papers by this authorSebastian Beyer, Sebastian Beyer Division of Bioengineering and NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117542 (Singapore)Search for more papers by this authorProf. Thorsten Wohland, Prof. Thorsten Wohland Department of Chemistry, National University of Singapore, Singapore 117542 (Singapore)Search for more papers by this authorProf. Dieter Trau, Corresponding Author Prof. Dieter Trau [email protected] Division of Bioengineering and Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 117542 (Singapore) http://www.biosingapore.com Dieter Trau, Division of Bioengineering and Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 117542 (Singapore) http://www.biosingapore.com Daniel Lubrich, Physics Department and NanoCore, National University of Singapore, Singapore 117542 (Singapore), Fax: (+65) 6872-3069 http://www.nanocore.nus.edu.sg/daniel_lubrich.htmlSearch for more papers by this authorDr. Daniel Lubrich, Corresponding Author Dr. Daniel Lubrich [email protected] Physics Department and NanoCore, National University of Singapore, Singapore 117542 (Singapore), Fax: (+65) 6872-3069 http://www.nanocore.nus.edu.sg/daniel_lubrich.html Dieter Trau, Division of Bioengineering and Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 117542 (Singapore) http://www.biosingapore.com Daniel Lubrich, Physics Department and NanoCore, National University of Singapore, Singapore 117542 (Singapore), Fax: (+65) 6872-3069 http://www.nanocore.nus.edu.sg/daniel_lubrich.htmlSearch for more papers by this author Laiyi Lin, Laiyi Lin Physics Department and NanoCore, National University of Singapore, Singapore 117542 (Singapore), Fax: (+65) 6872-3069 http://www.nanocore.nus.edu.sg/daniel_lubrich.htmlSearch for more papers by this authorSebastian Beyer, Sebastian Beyer Division of Bioengineering and NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, Singapore 117542 (Singapore)Search for more papers by this authorProf. Thorsten Wohland, Prof. Thorsten Wohland Department of Chemistry, National University of Singapore, Singapore 117542 (Singapore)Search for more papers by this authorProf. Dieter Trau, Corresponding Author Prof. Dieter Trau [email protected] Division of Bioengineering and Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 117542 (Singapore) http://www.biosingapore.com Dieter Trau, Division of Bioengineering and Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 117542 (Singapore) http://www.biosingapore.com Daniel Lubrich, Physics Department and NanoCore, National University of Singapore, Singapore 117542 (Singapore), Fax: (+65) 6872-3069 http://www.nanocore.nus.edu.sg/daniel_lubrich.htmlSearch for more papers by this authorDr. Daniel Lubrich, Corresponding Author Dr. Daniel Lubrich [email protected] Physics Department and NanoCore, National University of Singapore, Singapore 117542 (Singapore), Fax: (+65) 6872-3069 http://www.nanocore.nus.edu.sg/daniel_lubrich.html Dieter Trau, Division of Bioengineering and Department of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 117542 (Singapore) http://www.biosingapore.com Daniel Lubrich, Physics Department and NanoCore, National University of Singapore, Singapore 117542 (Singapore), Fax: (+65) 6872-3069 http://www.nanocore.nus.edu.sg/daniel_lubrich.htmlSearch for more papers by this author First published: 12 November 2010 https://doi.org/10.1002/anie.200907321Citations: 5 † This work was funded by NanoCore at NUS and by NUS grant R397-000-077-112. T.W. acknowledges grant 07/1/21/19/488 (R-143-000-351-305) from BMRC Singapore. We thank Jesse Matthew Goldman and Johan Van Der Maarel for valuable suggestions and Hoon Hwee Teo for assistance in the wet lab. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract It's a trap! A simple process based on reverse-phase layer-by-layer encapsulation can be used to produce surface-bound semipermeable microenclosures that can trap biomolecules. Biomolecules such as nucleic acids and proteins can be encapsulated whilst preserving their functionality. Electrophoresis can be used to create sharp concentration gradients, and enzymatic reactions such as DNA digestion can be controlled by diffusion of ions into the microenclosures (see picture). Citing Literature Supporting Information Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description anie_200907321_sm_miscellaneous_information.pdf446.1 KB miscellaneous_information anie_200907321_sm_video_1.avi1.8 MB video_1 anie_200907321_sm_video_2.avi2.2 MB video_2 anie_200907321_sm_video_3.avi1.8 MB video_3 anie_200907321_sm_video_4.avi1.2 MB video_4 anie_200907321_sm_video_5.avi217.2 KB video_5 Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume49, Issue50December 10, 2010Pages 9773-9776 RelatedInformation

Mit einem einfachen Prozess auf Basis einer Umkehrphasen-Layer-by-Layer-Verkapselung wurden oberflächengebundene Mikrobehälter zum Einschluss und zur Untersuchung von Biomolekülen hergestellt. Biomoleküle wie DNA und Proteine wurden eingeschlossen, ohne dass ihre Biofunktionalität beeinflusst wurde. Durch Elektrophorese wurden Konzentrationsgradienten erzeugt, und enzymatische Raktionen in kleinen Reaktionsräumen wurden durch Eindiffusion von Ionen gesteuert.

Metastable two-stranded DNA loops can be assembled into extended DNA oligomers by kinetically controlled self-assembly. Along the designed reaction pathway, the sequence of hybridization reactions is controlled by progressively revealing toeholds required to initiate strand-displacement reactions. The product length depends inversely on seed concentration and ranges from a few hundred to several thousand base-pairs.

Continuous rotation of DNA around its phosphate backbone is achieved with a simple nanomotor, which is driven by an electric field oscillated between four orientations (see image). The motor consists of a DNA rotor and a partially single-stranded DNA axle held between a surface and a magnetic bead. Rotation is caused by realignment of the rotor DNA with the oscillated electric field.

Ziehen und Strecken: Aus DNA wurde eine molekulare Maschine konstruiert, bei der durch das Zusammenwirken vieler molekularer Pinzetteneinheiten eine Bewegung über größere Distanzen erreicht wird. Diese Maschine ist dazu in der Lage, sich wiederholt auf 75 % ihrer vollständig gestreckten Form zusammenzuziehen (siehe Bild). Angetrieben wird diese Bewegung durch zwei „Treibstoff“-Stränge.

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

We demonstrate the use of a one-dimensional template to control the shape of a two-dimensional array self-assembled from a minimal set of DNA tiles. A periodic single-stranded template seeds tile assembly. A unique vertex tile at the 5′ end of the template controls the positioning of edge and body tiles to create a wedge-shaped array. The vertex angle of the array is approximately 12°; edge lengths are of the order of 1μm.

We present a study of the hybridization of complementary DNA hairpin loops, with particular reference to their use as fuel for autonomous DNA devices. The rate of spontaneous hybridization between complementary hairpins can be reduced by increasing the neck length or decreasing the loop length. Hairpins with larger loops rapidly form long-lived kissed complexes. Hairpin loops may be opened by strand displacement using an opening strand that contains the same sequence as half of the neck and a "toehold" complementary to a single-stranded domain adjacent to the neck. We find loop opening via an external toehold to be 10-100 times faster than via an internal toehold. We measure rates of loop opening by opening strands that are at least 1000 times faster than the spontaneous interaction between hairpins. We discuss suitable choices for loop, neck, and toehold length for hairpin loops to be used as fuel for autonomous DNA devices.

We demonstrate the use of rolling circle replication to template linear DNA arrays whose sizes bridge the gap between nanometre-scale self-assembly and top-down lithographic fabrication. Using rolling circle replication we have produced an oligonucleotide containing several hundred repeats of a short sequence motif. On this template we have constructed, by self-assembly, an array consisting of two parallel duplexes periodically linked by antiparallel Holliday junctions. We have observed arrays up to 10 µm in length by atomic force microscopy.