Since April 2014, the University of Wisconsin–Madison (UW–Madison) has partnered with Nestlé China's Dairy Farming Institute (DFI) to create noncredit training for dairy industry professionals in China. UW–Madison faculty and staff created a competency-based curriculum model, trained DFI and industry partners in pedagogy, and helped Nestlé develop courses that are practical, hands-on, and science-based with a goal of consistency in content, evaluation, and excellence in course delivery. Entering its third year, the program is a success with invested partners, high quality assessments, and UW–Madison faculty learning competency-based models.

Two intensive forage processing mechanisms, utilising either shredding or impact processing, were used to investigate physical disruption of wilted alfalfa and whole-plant corn as a potential means to improve fibre digestion. Physical disruption was quantified by a processing level index (PLI) defined as the ratio of treatment leachate conductivity relative to that of an ultimately processed treatment. The goal was to achieve a PLI of at least 60%. Impact processing created more physical disruption than shredding, and the former method was able to achieve the desired PLI goal. Although impact processing significantly reduced particle size of both wilted alfalfa and whole-plant corn, more than 40% of alfalfa particles were longer than 6.3 mm when the PLI of greater than 60% was achieved. The mean particle-size of the kernel fraction of processed whole-plant corn was less than 1 mm after processing with the impact processor. Impact processing of wilted alfalfa significantly increased the rapidly soluble fraction and increased the rate of degradation of DM and potentially digestible fibre (pdNDF) during an in situ digestion experiment. Intensive mechanical processing has the potential to improve ruminant animal utilisation of forage crops through improved fibre digestion.

Abstract Three alfalfa biotypes were chosen based on the presumption that they would be sources of alfalfa herbage that differed in lignin concentration and therefore cell wall digestibility. The hypothesis was that a lesser lignin concentration would result in greater alfalfa neutral detergent fiber (NDF) digestibility and greater beef steer growth performance. The three alfalfa biotypes were HarvXtra (Forage Genetics International), Hi-Gest 360 (Alforex Seeds), and a control alfalfa, LegenDairy XHD (Winfield Solutions LLC). High-moisture wrapped bales were prepared from second-harvest, d 30 crops. Digestibility of NDF was determined using in vitro incubations and a steer digestibility trial. Alfalfa baleage and trace mineral salt were fed to Angus steers (300 kg initial body weight, 4 pens/treatment) in an 83-day growing-phase trial. Alfalfa acid detergent lignin concentrations were 75.6, 71.8, and 63.0 g/kg dry matter (P = 0.34) for LegenDairy, Hi-Gest and HarvXtra, respectively. Based on in vitro total-tract NDF digestibility coefficients, HarvXtra tended (P ≥ 0.09) to have the highest NDF digestibility. Alfalfa biotype affected in vivo apparent total tract digestibility of NDF (P < 0.001) and there was a trend for an effect on acid detergent fiber digestibility (P = 0.051). Hi-Gest and HarvXtra had similar in vivo apparent NDF digestibilities, which were greater than for LegenDairy (P < 0.05). There was no alfalfa biotype effect on daily alfalfa dry matter intake (DMI; P = 0.51) or average daily gain (P = 0.25) by growing steers. The absence of an effect by the novel alfalfa biotypes on DMI by growing steers suggests that the compositional and digestibility differences of the novel alfalfa biotypes compared to LegenDairy were not sufficient to alleviate the limitation of physical fill (if evident) on DMI. If more disparity in cell wall composition and NDF digestibility were to exist between control and reduced-lignin biotypes, then perhaps an advantage in cattle growth performance for a reduced-lignin alfalfa biotype would be detectable.

Undigested forage neutral detergent fiber (uNDF) from long-term ruminal in situ incubations are used to estimate indigestible neutral detergent fiber (iNDF). Measurement of iNDF is important in forage evaluation because it defines the potentially digestible pool of neutral detergent fiber (NDF). Near-infrared reflectance spectroscopy (NIRS) can be calibrated to in situ reference sets to rapidly predict uNDF. Our objective was to compare uNDF estimates after 240 h of incubation when two types of bags were used in the in situ reference method. The bags compared were 4 cm × 5 cm Ankom F57 bags (25 micron pore size), and 5 cm × 10 cm Ankom in situ bags (50 micron pore size). Alfalfa samples from Pennsylvania and Wisconsin (n = 144) of different varieties and harvest intervals were used. One-half or two gram samples, respectively, were weighed into the small and large bags in triplicate. Mass to surface area was 0.05 and 0.02 g/cm2 for the small and large bags, respectively. The iNDF content after 240 h incubation was evaluated by two types bags in three rumen-cannulated Holstein cows. Each dried and ground forage was also scanned to determine the visible–near-infrared-reflectance spectra with a FOSS 6500 spectrophotometer. Prediction equations were developed for each bag type using modified partial least square regressions. The estimated iNDF fraction from small and large bags were 13.75% and 9.97%, respectively (SED = 0.39, P < 0.001). The coefficient of determination for calibration (R2), cross-validation (1 - VR), calibration standard deviation (SEC), and interactive authentication standard deviation (SECV) was 0.94, 0.92, 0.85 and 0.98 for values determined with the small bag and 0.88, 0.85, 1.12 and 1.27 for iNDF for values determined with the large bag, respectively. Results indicate that iNDF varies among alfalfa cultivars and NIRS can be used to quickly and quantitatively estimate iNDF content in alfalfa. Bag type influences 240h NDF residues. NIRS predictions of iNDF from the small bag calibration set had higher R2 and lower SEC and SECV than the large bag calibrations.

The primary objective of this randomized field study was to assess the effect of antibiotic therapy on health and growth of preweaned dairy calves diagnosed with naturally occurring respiratory disease, using respiratory scoring and portable lung ultrasound. A secondary objective was to determine whether treatment response depended on clinical presentation at the time of diagnosis. Holstein calves (n = 357) were enrolled at 3 to 6 d of age and followed until 52 d on 2 commercial dairies. Calves were examined twice weekly by blinded members of the research team. Clinical respiratory and ultrasonographic lung scores were assigned at each exam and used to classify the first detected respiratory disease event (BRD1) into upper respiratory tract disease (clinical, no significant consolidation); subclinical lobular pneumonia (not clinical, patchy consolidation ≥1 cm2); clinical lobular pneumonia (clinical, patchy consolidation ≥1 cm2); subclinical lobar pneumonia (not clinical, ≥1 lobe consolidated); and clinical lobar pneumonia (clinical, ≥1 lobe consolidated). At BRD1, calves were blocked by their respiratory disease status and randomized to receive an antibiotic (tulathromycin, 2.5 mg/kg subcutaneous) or placebo (sterile saline, equal volume subcutaneous). Multivariable linear and logistic regression analyses were used to model response to therapy. At BRD1 (n = 289), the distribution of diagnoses was 29% (upper respiratory tract disease), 43% (subclinical lobular pneumonia), 13% (clinical lobular pneumonia), 8% (subclinical lobar pneumonia), and 7% (clinical lobar pneumonia). Early antibiotic therapy limited progression of lung consolidation immediately following treatment, reduced the likelihood of requiring treatment within 7 d of BRD1, and improved growth and mortality before weaning. Despite receiving multiple doses of antibiotics after BRD1, calves treated with either antibiotic or placebo were equally likely to enter the weaning phase with pneumonia. Clinical presentation was associated with response to treatment for worsening of consolidation, early treatment failure, days to retreatment, and average daily gain. Overall, treatment was associated with short-term benefits, but more research is needed to develop treatment protocols that more effectively treat pneumonia and ensure that calves enter the weaning period with ultrasonographically clean lungs.

Automated milk feeders offer flexibility to feed calves high milk allowances, to change the daily quantity of milk offered, and also to dispense additives like probiotics on an individual basis. Our objectives were to test the effects of 2 milk feeding protocols and a lactic acid bacterium probiotic on performance and behavior in calves. Heifer dairy calves (n = 96) were enrolled at birth in a 2 × 2 factorial study design comparing feeding (1) 2 milk feeding protocols and (2) a lactic acid bacterium-based probiotic program, or a placebo, using automated milk feeders. The early milk feeding strategy (EM) offered a maximum of 11 L/d on day 1 and a peak maximum allowance of 15 L/d on day 21. The late milk feeding strategy (LM) offered a maximum of 7 L/d on day 1 and increased slowly to its peak at 13 L/d on day 28. Both feeding strategies gradually weaned the calves after peak milk allowance until complete weaning at day 53, offering a total of 543 liters of milk. Probiotics or placebo were fed orally in a gel once after colostrum, and twice daily in the milk until weaning. Water and calf starter were provided ad libitum. The experimental period was divided into 3 periods: from day 1 on the automated feeder to day 28 (Period 1), from day 29 to day 53 (Period 2), and the week post-weaning (Period 3). For Period 1, the average daily gain (ADG) of the probiotic group was greater than that of the placebo group (0.84 ± 0.10 kg/d vs. 0.74 ± 0.10 kg/d, respectively), but was not different between milk feeding strategies. For Period 2, ADG was not affected by probiotic or milk feeding strategies. For Period 3, ADG was greater for EM compared to LM (1.27 ± 0.10 kg/d vs. 1.02 ± 0.10 kg/d, respectively), but not between probiotic and placebo groups. During the whole experimental period, LM calves consumed significantly more milk than the EM calves (431.84 ± 33.0 liters vs. 378.64 ± 34.2 liters, respectively). During Period 3, probiotics affected the frequency of visits to the calf starter feed bunk (37.72 ± 2.8 vs. 23.27 ± 2.8 visits per day for probiotic and placebo groups, respectively), but did not affect total time spent at the feed bunk. The supplementation of a lactic acid-based probiotic improved ADG during early life and altered some aspects of the feeding behavior of dairy calves. Calves receiving an early accelerated milk allowance had improved growth during post-weaning and consumed less milk in total, which may indicate better use of solid feed.

Kernza intermediate wheatgrass is the first perennial grain crop in the world and has been developed with conventional breeding to increase seed yield of forage intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey). When managed for dual-use (grain and forage), Kernza intermediate wheatgrass can produce grain, crop residue (straw) in the summer, and green forage in the spring and fall. Mixtures of this grass with legumes could increase forage yield and nutritive value and provide other environmental and economic benefits. Despite the growing interest in these dual-use production systems, forage nutritive value of Kernza intermediate wheatgrass forage in a dual-use system in the Upper Midwest is unknown. A replicated field experiment was established in two locations in southern Wisconsin (Arlington and Lancaster) with two treatments: Kernza intermediate wheatgrass grown in monoculture and mixture with red clover (Trifolium pratense L.). Forage samples were collected at late vegetative stage in the spring, at grain harvest in the summer, and at the end of the regrowth period in the fall. Forage nutritive value of the monoculture was greatest in the spring with 456, 249 and 225 g kg(-1) for neutral detergent fiber (NDF), acid detergent fiber (ADF) and crude protein (CP), respectively; lowest in the summer with 702, 427 and 51 g kg(-1) NDF, ADF and CP, respectively, and intermediate in the fall with 590, 337 and 119 g kg(-1) NDF, ADF and CP, respectively. Predicted total-tract neutral detergent fiber digestibility (ttNDFD) was 0.53 for the spring forage and averaged 0.37 for the summer and fall forage, with no differences between the mixture and monoculture. The relative forage quality (RFQ) for the monoculture was 175 for the spring forage, 65 for the summer residue, and 116 for the fall. Intercropping red clover with Kernza intermediate wheatgrass increased CP of the summer crop residue by 69%, and increased CP and RFQ of the fall forage by 49% and 11%, respectively, while reducing NDF and ADF of the fall forage by 25% and 18%, respectively. Therefore, Kernza intermediate wheatgrass forage is suitable for lactating beef cows, dairy cows, and growing heifers when harvested in the spring and fall, and it offers high potential for dual-use grain and forage systems.

This study compared dry matter (DM) predictions of 3 handheld near-infrared spectrophotometer (NIRS) units (Moisture Tracker, Digi-Star Inc., Fort Atkinson, WI) to conventional oven drying at 60°C using 2 alfalfa and 2 corn silages. In addition, on-farm DM methods [microwave, Koster tester (Koster Moisture Tester Inc., Brunswick, OH), and food dehydrator methods] were also compared. Corn and alfalfa silages (1,600 g) obtained from the University of Wisconsin Dairy Cattle Center (DCC) and the Arlington Research Station (ARS) were analyzed for DM daily for 20 d. Two NIRS calibration methods were also tested within each unit. The DM predicted from the factory-preset calibrations was NIRf. The adjusted DM prediction was NIRa, where the average difference between oven-dried and NIRf determined on duplicate forage samples for 3 d before the experiment was used as a bias adjustment for all subsequent DM determinations. The average predicted DM from the 20 scans was recorded as the forage DM. The process was repeated 3 times with each NIRS unit. Two 100-g subsamples of each forage were also oven-dried for 48 h at 60°C daily in a forced-air oven. Oven DM of ARS and DCC alfalfa silages were 37.3 ± 1.1% and 48.5 ± 1.9%, respectively (mean ± standard deviation). Oven DM of ARS and DCC corn silages were 34.7 ± 1.2% and 37.4 ± 0.5%, respectively (mean ± standard deviation). Dry matter determinations from NIRf were on average 3.5 units higher than the oven DM values. The NIRa DM predictions were on average 1.7 DM units lower than the oven DM values. Additionally, differences among the 3 NIRf probe results were detected (43.1, 40.7, and 41.3% DM, respectively), but all other results were similar between probes. Determinations of DM by the microwave and food dehydrator were also similar with the 60°C, 48-h oven method, whereas the Koster tester was lower than the oven. The handheld NIRS units more accurately predicted DM content of the alfalfa silage but were not as accurate with corn silages when the factory preset calibrations were corrected for bias.

The objective of this study was to compare neutral detergent fiber (NDF) digestibility of corn stover that had been treated by 2 alkali treatment methods. Two experiments were conducted to test a sodium hydroxide (NaOH) treatment method that uses an ethanol/water co-solvent (NaOH/ethanol-H2O, United States Patent No. 20140220228) and a calcium hydroxide (CaOH) treatment method, which uses water as a solvent (CaOH/H2O). An in situ trial was conducted to compare NDF digestion kinetics between NaOH/ethanol-H2O-treated stover, CaOH/H2O-treated stover, untreated corn stover, and soy hulls. The digestion rate of potentially digestible NDF (kd) of NaOH/ethanol-H2O-treated corn stover (5.36%/h) was higher than CaOH/H2O-treated stover (2.27%/h), or untreated corn stover (1.76%/h) and similar to the kd of soy hulls (4.93%/h). The indigestible NDF (iNDF) fraction of untreated corn stover (35.1% of NDF) was reduced by CaOH/H2O treatment (27.3% of NDF) and by NaOH/ethanol-H2O treatment (2.8% of NDF). The iNDF fraction in soy hulls (3.6% of NDF) was similar to iNDF of NaOH/ethanol-H2O-treated stover. An in vivo digestibility trial was also conducted to compare fiber digestibility of diets supplemented with untreated corn stover, NaOH/ethanol-H2O-treated corn stover, or soy hulls. Total-tract apparent dry matter (DM) and NDF digestibility were measured with 8 lactating Holstein cows in a replicated 4 × 4 Latin square with four 21-d periods. Apparent DM digestibility (DMD) was improved when supplemental soy hulls were added to the base diet (60.0% DMD) compared with the base diet with no supplemental fiber (57.7% DMD). Apparent DM digestibility was reduced when diets were supplemented with untreated stover (52.4%). Dry matter digestibility of NaOH/ethanol-H2O-treated stover was similar (54.8% DMD) to all other treatments. Digestibility of NDF was lowest when cows were fed the diet with supplemented untreated stover (35.5% of NDF), and improved when soy hulls (40.6% of NDF) or NaOH/ethanol-H2O-treated stover (43.8% of NDF) were added to the diets. The NaOH/ethanol-H2O treatment process improves the DM and NDF digestibility of corn stover to values similar to those of soy hulls.

The objective of this study was to evaluate 3 milk replacer (MR) feeding programs on calf performance up to 4 mo of age. Neonatal Holstein male calves (n = 48; 44 ± 1.5 kg BW) were randomly assigned to either a moderate rate of MR (MOD; 0.66 kg/d for 39 d then 0.33 kg/d for 3 d), an ad libitum rate of MR (ADLIB; offered twice daily between 0630 and 0830 h and between 1430 and 1630 h for 35 d, 0.66 kg/d for 4 d, and 0.33 kg/d for 3 d), or a step-up rate of MR (STEPUP; increased from 0.32 to 0.62 kg/d in first 12 d, 0.66 kg/d for 27 d, and 0.33 kg/d for 3 d). The MR (25% CP, 18% fat) was fed 2× daily up to d 39 and 1× daily thereafter. Texturized starter (40% starch, 21% CP) and water were offered ad libitum. Calves were housed in individual pens through d 56. Calf BW was measured initially and weekly thereafter. Hip widths (HW) were measured initially and every 2 wk thereafter. Feces were scored and feed intake recorded daily. From 56 to 112 d, calves were grouped by the 3 MR programs and moved to group pens (4 calves/ pen). The same starter blended with 5% chopped grass hay was offered ad libitum. BW and HW were measured on d 56, 84, and 112. Data, within period, were analyzed as a completely randomized design with repeated measures when appropriate. Experimental units were calf in first period and pen in second. Total MR intake per calf averaged 27, 51, and 25 kg for MOD, ADLIB, and STEPUP programs, respectively, with a range of 42 to 63 kg for ADLIB. In the first 56 d, starter intake and feed efficiency were less for ADLIB vs. MOD (P < 0.05), while fecal scores and abnormal fecal score days were greater for calves fed ADLIB vs. MOD (P < 0.05). Calves fed STEPUP had lesser ADG than calves fed MOD (P < 0.05). In the second 56 d, initial BW was greater for ADLIB vs. MOD (P < 0.05), though final BW was not different between MOD and ADLIB or STEPUP (P > 0.05). Calves previously fed MOD had greater ADG, feed efficiency, and HW change than calves fed ADLIB (P < 0.05). Though BW were greater at weaning when MR was fed ad libitum, calves were less efficient and growth advantages were lost by 4 mo of age.

This study compared DM predictions of three hand-held near infrared spectrophotometer (NIRS) units (Moisture Tracker, Digi-Star Inc., Fort Atkinson, WI) to conventional oven drying at 60°C using two alfalfa and two corn silages. Corn and alfalfa silages (1,500 g) obtained from the University of Wisconsin Dairy Cattle Center (DCC) and the Arlington Research Station (ARS) were analyzed for DM daily for 20 d. Three NIRS calibrations were also tested within each unit: NIRf, NIRa, and NIRb. The DM predicted from the factory-preset calibrations was NIRf. NIRa was an adjusted DM prediction, where the average difference between oven-dried forage and NIRf determined on duplicate forage samples for 3 d before the experiment was used as a bias adjustment for all subsequent DM determinations. NIRb was a bias-adjusted DM prediction based on the average difference between oven-dried forage and NIRf over the 20-d experiment. NIRb was determined on each forage sample after the experiment had been completed. Each forage was scanned 20 times by each NIRS unit. The average predicted DM from the 20 scans was recorded as the forage DM. The process was repeated three times with each NIRS unit. Two 100-g subsamples of each forage were then oven-dried for 48 h at 60°C daily. Data was analyzed using PROC MIXED (SAS, version 9.3), with method, day, feed, method × day, and method × feed as fixed effects and equipment as the random effect. Oven DM of ARS and DCC alfalfa silages was 37.34 and 48.52%, respectively. Oven DM of ARS and DCC corn silages was 34.69 and 37.41% DM, respectively. NIRf DM predictions were significantly different from their respective oven values for ARS corn silage and ARS haylage (P < 0.0001), and NIRf DM tended to differ from oven DM for DCC Corn Silage; P = 0.06). NIRf and oven DM for DCC haylage were similar (P = 0.99). All NIRb DM values were similar to oven-dried DM for all four forages (P > 0.05). The hand-held NIRS units accurately predicted DM content of the alfalfa and corn silages when the factory preset calibrations were corrected for bias.

The objective of this trial was to evaluate, in dairy cattle, the effects of calcium hydroxide treatment of whole-plant corn and a treatment applied to the bottom stalk fraction of the corn plant, achieved by harvesting corn in 2 crop streams. The treatments were calcium hydroxide–treated corn silage (TRTCS), toplage supplemented with calcium hydroxide–treated stalklage (TPL), a positive control of brown midrib corn silage (BMR), and a negative control of conventional whole-plant corn silage (WPCS). The toplage was harvested at a height of 82 cm with 2 of the 6 rows set as ear-snapping to incorporate higher tissues into the stalklage. Stalklage was harvested at 12 cm, and other corn silages were harvested at 27 cm. Sixteen pens, each with 8 Holstein cows averaging 70±25 d in milk and 46±11 kg of milk d−1, were assigned 4 per treatment in a completely randomized design. The diet was approximately 40% corn silage, 20% alfalfa silage, and 40% concentrate on a dry matter basis. A 2-wk covariate period with conventional corn silage was followed by an 8-wk treatment period in which the 4 corn silage treatments were the only effective difference in diets. Cows fed TPL and TRTCS consumed more (1.9 and 1.4 kg of organic matter d−1, respectively) than did cows fed WPCS. Milk yield was greater for cows fed BMR, TPL, and TRTCS. Cows fed BMR and TPL produced 2.9 and 2.7 kg d−1, respectively, more energy-corrected milk (ECM) than cows fed WPCS, and cows fed TRTCS had the greatest ECM production (4.8 kg of ECM d−1 greater than cows fed WPCS). No differences in body weight or body condition scored were observed. Milk fat concentration was similar among treatments and milk protein concentration was reduced for TRTCS. Starch and neutral detergent fiber digestibility were greater for cows fed TRTCS.

Alkaline treatment of gramineous crop residues can convert an abundant, minimally utilized, poorly digestible straw into a moderately digestible feedstuff. Given the volatile nature of grain prices, substitution of treated stover for grain was investigated with dairy cows to provide insights on ruminal and digestibility effects of a feed option that makes use of alternative, available resources. The objective of this study was to evaluate changes in diet digestibility and ruminal effects when increasing levels of calcium oxide-treated corn stover (CaOSt) were substituted for corn grain in diets of lactating cows. Mature corn stover was treated with calcium oxide at a level of 50g∙kg−1 dry matter (DM), brought up to a moisture content of 50% following bale grinding, and stored anaerobically at ambient temperatures for greater than 60d before the feeding experiment. Eight ruminally cannulated Holstein cows averaging 686kg of body weight and 35kg of milk∙d−1 were enrolled in a replicated 4×4 Latin square, where CaOSt replaced corn grain on a DM basis in the ration at rates of 0, 40, 80, and 120g∙kg−1 DM. All reported significant responses were linear. The DM intake declined by approximately 1kg per 4% increase in CaOSt inclusion. With increasing replacement of corn grain, dietary neutral detergent fiber (NDF) concentration increased. However, rumen NDF turnover, NDF digestibility, NDF passage rate, and digestion rate of potentially digestible NDF were unaffected by increasing CaOSt inclusion. Total-tract organic matter digestibility declined by 5 percentage units over the range of treatments, approximately 1.5 units per 4-percentage-unit substitution of CaOSt for grain. With increasing CaOSt, the molar proportions of butyrate and valerate declined, whereas the lowest detected ruminal pH increased from 5.83 to 5.94. Milk, fat, and protein yields declined as CaOSt increased and DM intake declined with the result that net energy in milk declined by approximately 1 Mcal per 4% increase in CaOSt. Time spent ruminating (min∙kg−1 DM intake) increased with increasing CaOSt, though total minutes per day were unaffected. These insights on the effect of substitution of treated corn stover for corn grain may be used to predict the effect on nutrient supply to the cow over a range of substitution levels. The acceptability of the effect will depend on the economics of milk production and availabilities of feedstuffs.

The effect of cow and in situ bag type on undigested NDF (uNDF) content of corn silage, wheat straw, and alfalfa silage after 240 h in situ incubation were evaluated in three rumen-cannulated Holstein cows. Two corn silages, a wheat straw, and an alfalfa silage sample were dried and ground to pass through a 2.5-mm screen. After thorough mixing, 1 , 2, and 5 g of each forage were placed into F57 Ankom bags (4 by 5 cm), Ankom 5- by 10-cm nylon bags (part number R0510), or Ankom 10- by 20-cm nylon bags (part number R1020), respectively. There were three duplicates for each treatment within each cow. Sample mass to surface area by bag was 25, 20, and 12.5 mg cm−2, respectively. The residual NDF was analyzed after 240 h incubation. Data was analyzed by SPSS 19.0. Within each forage, the effect of cow and bag type on uNDF within forages was determined by ANOVA. Means were compared by Duncan's multiple range test. The contents of the uNDF significantly differed due to bag type (P < 0.001). The content of uNDF in F57 bags was significantly higher than in R0510 and R1020 nylon bags (P < 0.05) and there was no significant difference in uNDF between R0510 and R1020 nylon bags (P > 0.05). The uNDF differed within cow for wheat straw (P < 0.001). Bag type and effective surface area should be taken into consideration when measuring uNDF by in situ methods; the estimate of uNDF in relatively indigestible materials, such as wheat straw, may also be affected by cow.

Ruminal digestion of neutral detergent fiber (NDF) is affected in part by the proportion of NDF that is indigestible (iNDF), and the rate at which the potentially digestible NDF (pdNDF) is digested. Indigestible NDF in forages is commonly determined as the NDF residue remaining after long-term in situ or in vitro incubations. Rate of pdNDF digestion can be determined by measuring the degradation of NDF in ruminal in vitro or in situ incubations at multiple time points, and fitting the change in residual pdNDF by time with log-transformed linear first order or nonlinear mathematical treatments. The estimate of indigestible fiber is important because it sets the pool size of potentially digestible fiber, which in turn affects the estimate of the proportion of potentially digestible fiber remaining in the time series analysis. Our objective was to compare estimates of iNDF based on in vitro (IV) and in situ (IS) measurements at 2 fermentation end points (120 and 288h). Further objectives were to compare the subsequent rate, lag, and estimated total-tract NDF digestibility (TTNDFD) when iNDF from each method was used with a 7 time point in vitro incubation of NDF to model fiber digestion. Thirteen corn silage samples were dried and ground through a 1-mm screen in a Wiley mill. A 2×2 factorial trial was conducted to determine the effect of time of incubation and method of iNDF analysis on iNDF concentration; the 2 factors were method of iNDF analysis (IS vs. IV) and incubation time (120 vs. 288h). Four sample replicates were used, and approximately 0.5g/sample was weighed into each Ankom F 0285 bag (Ankom Technology, Macedon, NY; pore size=25 µm) for all techniques. The IV-120 had a higher estimate of iNDF (37.8% of NDF) than IS-120 (32.1% of NDF), IV-288 (31.2% of NDF), or IS-288 technique (25.7% of NDF). Each of the estimates of iNDF was then used to calculate the rate of degradation of pdNDF from a 7 time point in vitro incubation. When the IV-120 NDF residue was used, the subsequent rates of pdNDF digestion were fastest (2.8% h(-1)) but the estimate of lag was longest (10.3h), compared with when iNDF was based on the IS-120 or IV-288 NDF residues (rates of 2.3%h(-1) and 2.4%h(-1); lag times of 9.7 and 9.8 h, respectively). Rate of pdNDF degradation was slowest (2.1% h(-1)) when IS-288 NDF residue was used as the estimate of iNDF. The estimate of lag based on IS-288 (9.4h) was similar to lag estimates calculated when IS-120 or IV-288 were used as the estimate of iNDF. The TTNDFD estimates did not differ between treatments (35.5%), however, because differences in estimated pools of iNDF resulted in subsequent changes in rates and lag times of fiber digestion that tended to cancel out. Estimates of fiber digestion kinetic parameters and TTNDFD were similar when fit to either the linear or nonlinear fiber degradation models. All techniques also yielded estimates of iNDF that were higher than predicted iNDF based on the commonly used ratio of 2.4 × lignin.