Mole crickets can become serious pests of turfgrasses, pastures, and vegetable seedlings. The first step in determining if you have a mole cricket problem at a site is to compare the existing damage to pictures of known mole cricket damage. If the damage is likely caused by mole crickets, specimens should be obtained and the pest identified. You then should determine if the number of mole crickets is great enough to cause an unacceptable level of damage and decide what control measures should be used. Eventually, a long-term, sustainable integrated pest management (IPM) program should be established. This 20-page guide will help you identify mole cricket infestations and manage them effectively and economically while minimizing environmental impacts. Written by C. R. Kerr, N. C. Leppla, E. A. Buss, and J. H. Frank, and published by the UF Department of Entomology and Nematology, May 2014. IPM-206/IN1021: Mole Cricket IPM Guide for Florida (ufl.edu)

To safely solve a pest problem, growers and pesticide applicators must be aware of the potential impacts of some pest-control strategies on bees, other pollinators, and beneficial arthropods. This 14-page fact sheet written by J. D. Ellis, J. Klopchin, E. Buss, and others and published by the UF/IFAS Entomology and Nematology Department explains the issue and provides strategies to protect honey bees and other beneficial insects from pesticides. https://edis.ifas.ufl.edu/in1027

Twenty nine new species of cynipid oak gall wasps from the Nearctic region (America north of Mexico) are described: Andricus archboldi Melika & Abrahamson, sp. nov., A. catalinensis Melika, Nicholls & Stone, sp. nov., A. chapmanii Melika & Abrahamson, sp. nov., A. chiricahuensis Melika, Nicholls & Stone, sp. nov., A. coconinoensis Melika, Nicholls & Stone, sp. nov., A. columbiensis Melika, Nicholls & Stone, sp. nov., A. cooki Melika, Nicholls & Stone, sp. nov., A. fitzpatricki Melika & Abrahamson, sp. nov., A. highlandensis Melika, Nicholls & Stone, sp. nov., A. mellificus Nicholls, Stone & Melika, sp. nov., A. menkei Melika & Abrahamson, sp. nov., A. mogollonensis Melika, Nicholls & Stone, sp. nov., A. nichollsi Melika & Stone, sp. nov., A. schickae Nicholls, Melika & Stone, sp. nov., A. torreyaensis Melika & Abrahamson, sp. nov., A. williami Melika, Nicholls & Stone, sp. nov., Antron lovellae Melika, Nicholls & Stone, sp. nov., A.tomkursari Melika, Nicholls & Stone, sp. nov., Dryocosmus archboldi Melika & Abrahamson, sp. nov., Loxaulus virginianae Melika & Buss, sp. nov., Neuroterus alexandrae Nicholls & Melika, sp. nov., N. aliceae Melika, Nicholls & Stone, sp. nov., N. bussae Melika & Nicholls, sp. nov., N. oblongifoliae Nicholls, Stone & Melika, sp. nov., N. quaili Melika, Nicholls & Stone, sp. nov., N. rosieae Melika, Nicholls & Stone, sp. nov., N. stonei Melika & Nicholls, sp. nov., Zapatella abrahamsoni Melika, sp. nov., Z. brooksvillei Melika & Abrahamson, sp. nov.. Alternate asexual and sexual generations are described for four species, Andricus archboldi Melika & Abrahamson, sp. nov., A. fitzpatricki Melika & Abrahamson, sp. nov., A. schickae Nicholls, Melika & Stone, sp. nov., Neuroterus aliceae Melika, Nicholls & Stone, sp. nov.. Descriptions, diagnoses, plus information on biology and host associations are given for all new species. All taxa are supported by morphological data; matching of generations is established using DNA sequence data. We also demonstrate that Neuroterus niger var. alimas Kinsey should be considered as a nomen dubium.

Are lawn caterpillars doing your mowing for you? Young caterpillars, or larvae, injure turfgrass by chewing notches along the edge of the leaves. This creates a ragged appearance (Figure 1) that may be hard to notice at first. Mature caterpillars eat a lot before they pupate and consume patches of turfgrass down to the crown. Because the turf looks scalped so quickly, people think that the damage occurs “overnight.” Several caterpillar species can be turfgrass pests, including the tropical sod webworm, the fall armyworm, and the striped grass looper. This document is ENY-352 (IN608), one of a series of the Department of Entomology and Nematology, UF/IFAS Extension. Original publication date: April 2006.

Nylanderia fulva (Mayr) (Hymenoptera: Formicidae), or tawny crazy ant, is an invasive ant from South America that is spreading in the southern US. Extremely large populations of this ant can inundate urban and natural landscapes, and efficient control methods are lacking. This study was conducted to determine if activity of N. fulva would decline after controlling the hemipteran honeydew-producers they were tending. Potted plants infested with cottony cushion scale (Icerya purchasi Maskell) (Hemiptera: Monophlebidae) were treated with 2 imidacloprid insecticide formulations, and changes in N. fulva survival and foraging behavior were monitored. There were fewer ant trails, lower trailing intensity, less foraging, and less nesting in potted plants treated with either product than in the control pots. The use of low application rates of systemic insecticide to reduce honeydew-producing hemipterans, such as cottony cushion scale, could be an important component of the integrated pest management of N. fulva.

In North America there are 32 species of Kermesidae in five genera, but in northeastern North America there are only nine species in four genera (Eriokermes, Nanokermes, Allokermes, and Kermes). The Allokermes spp. that are of economic importance in Florida are A. cueroensis (Cockerell), A. galliformis (Riley), and A. kingii (Cockerell). The kermes scale, Allokermes kingii (Cockerell), is an important pest on oak (Quercus spp.) trees. This document is EENY-338, one of a series of Featured Creatures from the Entomology and Nematology Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida. Published: November 2004. EENY338/IN615: Northern Red-Oak Kermes (suggested common names), Allokermes kingii (Cockerell) (Insecta: Hemiptera: Coccoidea: Kermesidae) (ufl.edu)

Southern chinch bug, Blissus isularis Barber, is the most damaging insect pest of St. Augustinegrass in the United States. St. Augustinegrass is the most common turfgrass used in Florida. The ubiquity of this single turfgrass species makes southern chinch bug an economically important pest in the state. In fact, chinch bugs cost Florida homeowners and professionals millions of dollars every year. This 7-page fact sheet written by Eileen A. Buss, Brianna M. Whitman, and Adam G. Dale and published by the UF/IFAS Department of Entomology and Nematology describes the biology of the pest and the damage it causes and lists ways to scout and monitor for chinch bugs and some strategies for control of the pest.https://edis.ifas.ufl.edu/lh036

Many people are seeking available and effective options that are safer for people and the environment than some conventional synthetic pesticides. There is also rising interest in organic gardening, which relies on many natural pesticides. Natural products can be used in isolation or combination with conventional pesticide programs as valuable rotation options, delaying or preventing onset of insect and disease resistance caused by repeatedly using the same chemical controls. This publication describes natural products used in residential landscapes and gardens that are generally less toxic to non-target organisms and the environment, and when used correctly, can be effective tools for plant protection. These products are most effective when used in an integrated pest management (IPM) program along with sanitation, proper cultural or maintenance practices, mechanical control tactics, use of resistant plant varieties, and biological control, when possible.

Whiteflies are a common pest of many ornamental plants throughout Florida and the world. There are more than 1,500 species worldwide and over 75 reported in Florida. Some of the most economically important species in Florida are the sweetpotato whitefly, also called the silverleafwhitefly (Bemisia tabaci), the ficus whitefly (Singhiella simplex), and the citrus whitefly (Dialeurodes citri). Although infestation does not always require treatment, it is important to be able to identify and monitor for these pests for effective integrated pest management. This 8-page fact sheet describes whitefly species, their identification and biology, the damage they cause, and best management practices to control them and still maintain healthy populations of natural enemies and other beneficial insects.

BACKGROUNDMole crickets (Neoscapteriscus spp.) consume turfgrasses and pasture grasses and uproot plants by their tunneling, which is detrimental to turf aesthetics and decreases forage quantity and quality. Insecticides are frequently used to prevent damage. In typical field trials, damage symptoms, not percent mortality or achieved level of control, are used to assess treatment efficacy. Here, however, laboratory tests assessed the direct effect of key insecticides on Neoscapteriscus mole cricket behavior.RESULTSMole crickets, Neoscapteriscus spp., were able to detect and avoid areas treated with fipronil [formulated product (FP)] and imidacloprid (FP). They tunneled less in sand treated with fipronil and avoided sand treated with fipronil and imidacloprid if given a choice. Mole crickets escaped areas treated with acephate, bifenthrin and fipronil. Bifenthrin and acephate caused increased tunneling during the first 90 min of observation. Fipronil and imidacloprid significantly reduced overall tunneling on treated areas.CONCLUSIONTested insecticides elicited two types of behavioral changes in Neoscapteriscus mole crickets: increased locomotory activity and tunneling [acephate (organophosphate) and bifenthrin (pyrethroid)] and reduced spatial movement [fipronil (phenylpyrazole) and imidacloprid (neonicotinoid)]. These behavioral responses resulted mainly from contact chemoreception and inherent neurotoxicity of the chemicals on Neoscapteriscus mole crickets. © 2017 Society of Chemical Industry.

Turfgrass is grown in many environments and for different uses, including home lawns, parks, athletic fields, cemeteries, golf courses, sod farms, pastures, and right-of-ways. The intensity of turfgrass insect management largely depends on the turf species, variety, and its intended use. This revised 20-page fact sheet describes how to manage a variety of insect pests including armyworms, bermudagrass mite, cutworms, fire ants, ground pearls, hunting billbug, mole crickets, scales/mealybugs, southern chinch bug, twolined spittlebugs, tropical sod webworm, and white grubs. Written by Eileen A. Buss and Adam G. Dale, and published by the Entomology and Nematology Department, March 2016. ENY-300/IG001: Insect Pest Management on Turfgrass (ufl.edu)

Plant growth regulators are known to influence a plant's characteristics and therefore can be used as an alternative strategy in making Blissus insularis Barber (Hemiptera: Blissidae) habitat unfavorable for this insect's survival. The reduction of B. insularis population densities following the application of mefluidide and trinexapac-ethyl in field plots indicated the indirect effect of plant growth regulator application on B. insularis for its management in St. Augustinegrass.

This EDIS publication is an alternate version of a page published first on the Featured Creatures website. The Featured Creatures collection provides in-depth profiles of insects, nematodes, arachnids and other organisms relevant to Florida. These profiles are intended for the use of interested laypersons with some knowledge of biology as well as academic audiences. This 6-page fact sheet that discusses the bluegrass billbug was written by Luis F. Aristizábal and Eileen Buss, and published by the UF Entomology and Nematology Department, April 2016. EENY-653/IN1133: Bluegrass Billbug Sphenophorus parvulus Gyllenhal (Insecta: Coleoptera: Curculionidae: Dryophthorinae) (ufl.edu)

Every landscape manager has a pest management toolbox, which contains tools that represent different management strategies. People can be quick to use pesticides, but an integrated approach using multiple tools can be much safer, have longer lasting beneficial effects, and in some cases cut costs. This revised 5-page fact sheet will help Extension agents and specialists, lawn and landscape managers, Florida Master Gardeners, and homeowners develop long-term sustainable pest management programs using an Integrated Pest Management (IPM) framework. Written by Eileen Buss and Adam G. Dale, and published by the Department of Entomology and Nematology, April 2016. ENY-298/IN109: Landscape Integrated Pest Management (ufl.edu)

The plant-phloem-feeding Blissus insularis possesses specialized midgut crypts, which harbor a dense population of the exocellular bacterial symbiont Burkholderia. Most individual B. insularis harbor a single Burkholderia ribotype in their midgut crypts; however, a diverse Burkholderia community exists within a host population. To understand the mechanism underlying the consistent occurrence of various Burkholderia in B. insularis and their specific association, we investigated potential gut symbiont transmission routes. PCR amplification detected a low titer of Burkholderia in adult reproductive tracts; however, fluorescence in situ hybridization assays failed to produce detectable signals in these tracts. Furthermore, no Burkholderia-specific PCR signals were detected in eggs and neonates, suggesting that it is unlikely that B. insularis prenatally transmits gut symbionts via ovarioles. In rearing experiments, most nymphs reared on St. Augustinegrass treated with cultured Burkholderia harbored the cultured Burkholderia strains. Burkholderia was detected in the untreated host grass of B. insularis, and most nymphs reared on untreated grass harbored a Burkholderia ribotype that was closely related to a plant-associated Burkholderia strain. These findings revealed that B. insularis neonates acquired Burkholderia primarily from the environment (i.e., plants and soils), even though the possibility of acquisition via egg surface cannot be excluded. In addition, our study explains how the diverse Burkholderia symbiont community in B. insularis populations can be maintained.

ABSTRACT The phloem-feeding Southern chinch bug, Blissus insularis, harbors a high density of the exocellular bacterial symbiont Burkholderia in the lumen of specialized midgut crypts. Here we developed an organ culture method that initially involved incubating the B. insularis crypts in osmotically balanced insect cell culture medium. This approach enabled the crypt-inhabiting Burkholderia spp. to make a transition to an in vitro environment and to be subsequently cultured in standard bacteriological media. Examinations using ribotyping and BOX-PCR fingerprinting techniques demonstrated that most in vitro-produced bacterial cultures were identical to their crypt-inhabiting Burkholderia counterparts. Genomic and physiological analyses of gut-symbiotic Burkholderia spp. that were isolated individually from two separate B. insularis laboratory colonies revealed that the majority of individual insects harbored a single Burkholderia ribotype in their midgut crypts, resulting in a diverse Burkholderia community within each colony. The diversity was also exhibited by the phenotypic and genotypic characteristics of these Burkholderia cultures. Access to cultures of crypt-inhabiting bacteria provides an opportunity to investigate the interaction between symbiotic Burkholderia spp. and the B. insularis host. Furthermore, the culturing method provides an alternative strategy for establishing in vitro cultures of other fastidious insect-associated bacterial symbionts. IMPORTANCE An organ culture method was developed to establish in vitro cultures of a fastidious Burkholderia symbiont associated with the midgut crypts of the Southern chinch bug, Blissus insularis. The identities of the resulting cultures were confirmed using the genomic and physiological features of Burkholderia cultures isolated from B. insularis crypts, showing that host insects maintained the diversity of Burkholderia spp. over multiple generations. The availability of characterized gut-symbiotic Burkholderia cultures provides a resource for genetic manipulation of these bacteria and for examination of the mechanisms underlying insect-bacterium symbiosis.

Core Ideas Cultural control of southern chinch bug in St. Augustinegrass by following recommended guidelines for mowing heights based on turfgrass cultivar, and practicing thatch management may reduce insecticide use and nontarget impacts. Turf parameters (turf height and thatch thickness) were closely associated to southern chinch bug densities observed in St. Augustinegrass residential lawns. Best management practices recommended for growing St. Augustinegrass in Florida may help to maintain a balance between turf health and pest pressure. Turf characteristics in conjunction with environmental factors influence the microclimate suitability for insect pest survival in grasses. In this study, we sought to find the association between southern chinch bug (Blissus insularis) densities, St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze] health characteristics (weed abundance, turf density and height, turf color, chlorophyll content, total Kjeldahl nitrogen concentration, and thatch thickness), and abiotic factors (soil pH, soil and air temperature, soil moisture, and light intensity) by sampling residential lawns in Alachua and Marion counties, Florida. A 2‐year study was conducted: approximately 90 St. Augustinegrass lawns were selected for monthly sampling in May and June 2011. This was followed by weekly sampling in 17 selected lawns in July and August 2011 and 13 lawns in June to August 2012, respectively. Results from lawn surveys indicated that light intensity (r = 0.30, P = 0.0034), and thatch thickness (r = 0.22, P = 0.0390) were positively associated with B. insularis densities. The inverse relationship between turf color and B. insularis densities indicated the impact of insect feeding damage. A significant positive association of insect abundance with grass height and thatch thickness identified the need for turf management practices (mowing at recommended cut height and verticutting/thatch removal) to reduce habitat suitability for insect pest survival.

The Southern chinch bug, Blissus insularis, possesses specialized midgut crypts that harbor dense populations of the exocellular symbiont Burkholderia. Oral administration of antibiotics suppressed the gut symbionts in B. insularis and negatively impacted insect host fitness, as reflected by retarded development, smaller body size, and higher susceptibility to an insecticide, bifenthrin. Considering that the antibiotics probably had non-lethal but toxic effects on host fitness, attempts were conducted to reduce gut symbionts using bacteriophage treatment. Soil-lytic phages active against the cultures of specific Burkholderia ribotypes were successfully isolated using a soil enrichment protocol. Characterization of the BiBurk16MC_R phage determined its specificity to the Bi16MC_R_vitro ribotype and placed it within the family Podoviridae. Oral administration of phages to fifth-instar B. insularis, inoculated with Bi16MC_R_vitro as neonates had no deleterious effects on host fitness. However, the ingested phages failed to impact the crypt-associated Burkholderia. The observed inactivity of the phage was likely due to the blockage of the connection between the anterior and posterior midgut regions. These findings suggest that the initial colonization by Burkholderia programs the ontogeny of the midgut, providing a sheltered residence protected from microbial antagonists.

Scale insects are a diverse group of piercing-sucking pests (Hemiptera) commonly found on ornamental plants in landscapes and nurseries. There are over 180 species of scale insects in Florida, but only a small percentage are important pests of ornamental plants. They damage plants and secrete a waxy covering that makes them difficult to control using most chemical control measures. This revised 6-page fact sheet differentiates between armored and soft-scale insect pests and lists common types of each, provides information about the biology of scale insects and how to identify them and the damage they cause, describes how to scout and monitor for scale insects, and lists several methods for prevention and control of scale insect invasions. Written by Eileen A. Buss and Adam Dale, and published by the Department of Entomology and Nematology, July 2016. ENY-323/MG005: Managing Scale Insects on Ornamental Plants (ufl.edu)

Invasive mole crickets (Orthoptera: Gryllotalpidae: Scapteriscus spp.) are destructive subterranean pests that cause significant economic losses for the turfgrass, sod and pasture industries. Their behavior suggests that they may sense insecticides applied to the soil, so we sought to examine and describe the antennal and palpal structures of S. vicinis Scudder, S. borellii Giglio-Tos, S. abbreviatus Scudder, and the native mole cricket, Neocurtilla hexadactyla Perty, by scanning and transmission electron micrography. The most abundant sensilla were antennal sensilla chaetica with mechanoreceptory and contact chemoreceptory functions. Each segment had olfactory sensilla basioconica and sensilla trichodea, sensilla coeloconica (with olfactory and thermo-hydroreceptory functions), and sensilla campaniformia (proprioreceptor). Sensilla on the mole cricket palps were non-pore or tip-pore, which suggests mechanoreceptory and contact chemoreceptory functions. Similar to other hemimetaboulous insects, mole cricket nymphs and adults have the same sensilla types. However, the number and size of antennomeres increased with each molt, allowing the antennae to accommodate more sensory sensilla as insects matured.