Таксономическое определение микроорганизмов, ассоциированных с диатомовыми водорослями, проводили комплексом методов микроскопии, микробиологии и филогенетического анализа. При культивировании на байкальской воде пресноводной диатомеи Synedra acus методами эпифлуоресцентной и электронной микроскопии показано, что бактерии колонизируют слизь, скрепляющую клетки живых микроводорослей, поверхность клеток, проникают внутрь панцирей погибших диатомей. Изолировано в чистую культуру и охарактеризовано 13 штаммов гетеротрофных бактерий. По результатам исследований морфологических, физиолого-биохимических признаков и анализа нуклеотидных последовательностей генов 16S рРНК изолированные штаммы определены до вида или рода – Sphingomonas sp., Variovorax paradoxus, Pseudomonas fluorescens, Microbacterium trichotecenolyticum, Rhodococcus sp., Caulobacter vibrioides, Brevundimonas vesicularis.

: Diatoms significantly differ from other groups of heterokont algae in the diversity of chloroplast forms, their number, and location in a cell and in the structure of pyrenoids. The information on the fine structure of chloroplast is recognized to be important for taxonomic and phylogenetic studies of diatoms. Six species of diatoms belonging to different classes have been examined using transmission electron microscopy. New data on the structure of chloroplast have been obtained and characteristics of pyrenoid ultrastructure of diatoms belonging to different phylogenetic clades have been specified. The results enlarge the data obtained earlier and specify a set of features of chloroplast ultrastructure for different phylogenetic clades of diatoms.

The kinetics of the free-radical copolymerization of styrene with divinyl sulfide in the presence of N,N′-bis(vinyloxyethyl)thiuram disulfide as an iniferter has been studied. The rate of polymerization is shown to decrease with increasing iniferter concentration. The structure of the copolymers isolated in the course of copolymerization has been investigated by electron microscopy and compared with that of the copolymers synthesized using AIBN as an initiator. Evolution in the morphology of the polymer phase during the process, which consists in self-organization of the secondary supramolecular structure into spheroids with diameters of 0.1–10 μm, has been revealed. The stages of the formation of polymer particles of various structures are described by the scheme of morphogenesis.

A diatom Synedra acus subsp. radians (Kotz.) Skabitsch. has been studied by transmission electron microscopy. Examination of ultrathin sections demonstrated that silica dissolution in ammonium fluoride pH 5 under mild conditions leaves the key ultrastructural elements intact. The ultrastructure and arrangement of the cell organelles was studied during ontogeny. Silicalemma-surrounded silica deposition vesicles (SDVs) with maturating daughter valves and forming girdle bands have been identified. This method of SDV visualization offers considerable advantages over the standard approach without silica dissolution.