Equine Veterinary EducationVolume 12, Issue 6 p. 333-336 Equine serum biochemical analyte comparisons on the Boehringer Mannheim/Hitachi 911 and Abaxis VetScan clinical chemistry analysers H. Dawson, H. Dawson Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 6R8, Canada.Search for more papers by this authorA. Sutton-Harmer, A. Sutton-Harmer Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 6R8, Canada.Search for more papers by this authorB. Hoff, B. Hoff Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 6R8, Canada.Search for more papers by this authorE. Grift, E. Grift Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 6R8, Canada.Search for more papers by this author H. Dawson, H. Dawson Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 6R8, Canada.Search for more papers by this authorA. Sutton-Harmer, A. Sutton-Harmer Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 6R8, Canada.Search for more papers by this authorB. Hoff, B. Hoff Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 6R8, Canada.Search for more papers by this authorE. Grift, E. Grift Animal Health Laboratory, Laboratory Services Division, University of Guelph, Guelph, Ontario N1H 6R8, Canada.Search for more papers by this author First published: 05 January 2010 https://doi.org/10.1111/j.2042-3292.2000.tb00071.xAboutPDF ToolsExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Volume12, Issue6December 2000Pages 333-336 RelatedInformation

The objective of this study was to compare and assess the agreement between the Coulter AcT Diff hematology analyzer (CAD) and the Bayer Technicon H1 (H1) using blood samples from 391 animals of 4 species. The H1 has been used in veterinary laboratories for many years. Recently, Coulter modified the CAD and added veterinary software for hematologic analysis of feline, canine, and equine samples. A comparison of hemograms from dogs, cats, horses, and cattle was made using EDTA-anticoagulated blood samples. Both instruments were calibrated using human blood products. Performance characteristics were excellent for most values. The exceptions were MCV in canine samples (concordance correlation of.710), platelet counts for feline and equine samples (.258 and.740, respectively), feline and bovine WBC counts (.863 and.857, respectively), and bovine hemoglobin (.876).

The purpose of this study was to assess agreement between a wet reagent and a dry reagent analyzer. Thirteen analytes (albumin, globulin, alkaline phosphatase, alanine aminotransferase, amylase, urea nitrogen, calcium, cholesterol, creatinine, glucose, potassium, total bilirubin, and total protein) for both canine and feline serum were evaluated. Concordance correlations, linear regression, and plots of difference against mean were used to analyze the data. Concordance correlations were excellent for 8 of 13 analytes (r greater than or equal to 0.90); the correlations for albumin, potassium, and calcium were clinically unreliable. The linear regression analysis revealed that several analytes had slopes significantly different from unity, which was likely related to methodological differences. Compared to the wet reagent analyzer, the dry reagent analyzer showed excellent agreement for alkaline phosphatase, alanine aminotransferase, amylase (feline), urea nitrogen, cholesterol, creatinine, glucose, total bilirubin (canine), and total protein. However, it showed only slight to substantial agreement for amylase (canine), calcium, albumin, potassium, and total bilirubin (feline).

Blood samples from 33 dogs, 28 cats, 24 horses and 25 cattle were analysed in duplicate on the Coulter Counter S-Plus IV and the Baker 9000 multichannel haematology analysers. The precisions of each instrument and the correlations between the instruments were evaluated. The precisions of both systems were good but, the Baker 9000 system showed more variability between duplicate determinations for all parameters. The Baker 9000 had better precision for white blood cell count, haemoglobin concentration, and MCV. Correlations between the two instruments were excellent except for MCHC. Neither analyser reliably provided feline platelet counts and the Baker 9000 also failed to report error messages for 8 of 10 apparently false platelet counts. The Coulter instrument consistently reported higher haemoglobin concentrations and higher feline and lower canine white blood cell counts than the Baker 9000.

Blood samples from 33 dogs, 28 cats, 24 horses and 25 cattle were analysed in duplicate on the Coulter Counter S-Plus IV and the Baker 9000 multichannel haematology analysers. The precisions of each instrument and the correlations between the instruments were evaluated. The precisions of both systems were good but, the Baker 9000 system showed more variability between duplicate determinations for all parameters. The Baker 9000 had better precision for white blood cell count, haemoglobin concentration, and MCV. Correlations between the two instruments were excellent except for MCHC. Neither analyser reliably provided feline platelet counts and the Baker 9000 also failed to report error messages for 8 of 10 apparently false platelet counts. The Coulter instrument consistently reported higher haemoglobin concentrations and higher feline and lower canine white blood cell counts than the Baker 9000.

Alterations of acid-base status, and fluid and electrolyte balance subsequent to exercise in Thoroughbred racehorses in North America have not been well-characterized. Des-cribed here are the results of an observational study conducted to characterize changes in fluid and electrolytes following strenuous exercise of 16 Thoroughbreds under routine training conditions. Changes following strenuous exercise were determined for the following variables: serum concentrations of sodium (Na), potassium (K), chloride (Cl) and protein; pH of blood; osmolality of plasma and urine; body weight; and, fractional urinary excretion (FE) of Na, K and Cl. The following changes occurred during exercise: increased concentration of Na in blood; increased FE of Na; decreased concentration of Cl in blood; decreased FE of Cl; increased urinary and plasmal osmolality; weight-loss; decreased pH of blood; and, increased concentration of lactic acid. The concurrent decreased concentration of chloride in plasma and acidemia in these horses differed from the hypochloremic, metabolic alkalosis previously described among endurance horses. Acidemia was attributed to production of lactic acid by anaerobic glycolysis.

Interferences caused by bilirubin, hemolysis, and lipemia on 25 clinical chemistry analytes in bovine, canine, equine, and feline sera were studied using the Coulter Dacos and commercial reagents. We present the data as "interferograms", which show the anticipated percent change in serum analyte activity or concentration with varying concentrations of bilirubin, hemoglobin, or lipid. Obvious species differences in response to at least one added interfering substance were found for alanine aminotransferase, aspartate aminotransferase, cholesterol, creatine kinase, globulin, total protein, and urea. The remaining analytes were affected in a linear or complex dose-response relationship or were only affected at the highest concentrations of interfering substances. These data will be useful in aiding interpretation of laboratory test results when common interferences are present in the serum.

The effects of acetoacetic acid, acetone, bilirubin, beta-carotene, three cephalospoprin antibiotics, glucose, hemoglobin and lipid on the kinetic Jaffé reaction and an enzymatic reaction for the determination of creatinine concentration were studied in bovine, canine, feline and equine serum. There were no obvious species' differences. The kinetic Jaffé reaction was unaffected by the addition of beta-carotene and hemoglobin. Acetone, cefazolin, cefoxitin, ceftiofur and glucose caused a positive bias while acetoacetic acid, bilirubin, and lipid caused a negative bias when added to the kinetic Jaffé reaction. The enzymatic reaction was unaffected by the addition of acetoacetic acid, acetone, beta-carotene, cefazolin, cefoxitin, glucose and hemoglobin while added lipid, bilirubin and ceftiofur caused a negative bias in the test results. Over all species and interferents, there was no difference in the precision of the two assay methods. In a series of sera from hospitalized patients, the two methods were highly correlated in a linear fashion. The enzymatic creatinine assay deals effectively with most interferents but has a greater cost and shorter shelf-life compared with the kinetic Jaffé reaction.

Eighteen analytes were compared using the Coulter Dacos and Kodak Ektachem DT-60 chemistry analyzers. All analytes were significantly linearly related. Correlations were excellent for fifteen analytes (r > 0.95); the correlations for sodium, chloride, and total protein were less than 0.95 but greater than 0.90. Several analytes had slopes significantly different from unity which was likely related to methodological differences. Regression equations are presented for the conversion of data generated using the Kodak Ektachem DT-60 to Coulter Dacos values so that, in the University of Guelph clinical pathology laboratory, one set of reference intervals serves both instruments. These data are directly applicable only in the laboratory in which they were developed. They should not be utilized directly in other laboratories, but can be used as general guidelines until confirmatory studies are done.