Colostrum deprived calves were experimentally infected with an adenovirus isolated from sheep and related to bovine adenovirus type 2. The calves showed respiratory symptoms and mild diarrhoea from the third day after infection. Laboratory tests revealed the development of leucopenia, lymphopenia, a drop of the pH of the urine and the appearance of pathological changes in the urine. The animals shed the virus in their nasal discharge, faeces and urine. Comparing the clinical and virological findings with the previous experimental infection of lambs it is concluded, that this type of adenovirus is similarly pathogenic for the two ruminant species.

Veterinary RecordVolume 158, Issue 7 p. 242-243 Short Communication Outbreak of enterocolitic salmonellosis on a wild pig farm R. Ecco DVM, MS, R. Ecco DVM, MS Program of Molecular Pathology (LMPDC), Universidade de Brasília, Campus Universitário Darcy Ribeiro, Cx Postal 04536, CEP 70910-900 Brasília, DF, BrazilSearch for more papers by this authorR. M. C. Guedes DVM, MS, PhD, R. M. C. Guedes DVM, MS, PhD Department of Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais-UFMG, Belo Horizonte, BrazilSearch for more papers by this authorE. Tury DVM, MS, PhD, E. Tury DVM, MS, PhD Pathology Division, União Pioneira da Integração Social, CEP 70390-125 Brasília, DF, BrazilSearch for more papers by this authorH. L. Santos Junior DVM, H. L. Santos Junior DVM Pathology Division, União Pioneira da Integração Social, CEP 70390-125 Brasília, DF, BrazilSearch for more papers by this authorS. Perecmanis DVM, MS, S. Perecmanis DVM, MS Department of Veterinary Microbiology, FAV, Universidade de Brasília, Brasília, BrazilSearch for more papers by this author R. Ecco DVM, MS, R. Ecco DVM, MS Program of Molecular Pathology (LMPDC), Universidade de Brasília, Campus Universitário Darcy Ribeiro, Cx Postal 04536, CEP 70910-900 Brasília, DF, BrazilSearch for more papers by this authorR. M. C. Guedes DVM, MS, PhD, R. M. C. Guedes DVM, MS, PhD Department of Clinic and Surgery, Veterinary School, Universidade Federal de Minas Gerais-UFMG, Belo Horizonte, BrazilSearch for more papers by this authorE. Tury DVM, MS, PhD, E. Tury DVM, MS, PhD Pathology Division, União Pioneira da Integração Social, CEP 70390-125 Brasília, DF, BrazilSearch for more papers by this authorH. L. Santos Junior DVM, H. L. Santos Junior DVM Pathology Division, União Pioneira da Integração Social, CEP 70390-125 Brasília, DF, BrazilSearch for more papers by this authorS. Perecmanis DVM, MS, S. Perecmanis DVM, MS Department of Veterinary Microbiology, FAV, Universidade de Brasília, Brasília, BrazilSearch for more papers by this author First published: 18 February 2006 https://doi.org/10.1136/vr.158.7.242Citations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article.Citing Literature Volume158, Issue7February 2006Pages 242-243 RelatedInformation

Four seronegative sows from a farm free of Aujeszky's disease (AD) were artificially inseminated and concurrently infected intranasally and intrauterinally with 105 dose of a virulent strain of Aujeszky's disease virus (ADV). The embryos were recovered surgically 5 d later. The virus could be re-isolated from nasal and vaginal swabs and from different parts of the genital organs (ovary, oviduct, uterus and cervix) of all infected sows. In 2 of the infected sows ADV was also found in the flushing medium used for embryo recovery. The results of virus isolation were confirmed by dot-blot hybridization and histological findings.Embryos with an intact zona pellucida (ZP) collected from the 4 donors were treated twice with 0.25% trypsin solution for 1 min each time prior to transfer into a seronegative sow. The recipient sow and her offspring remained ADV-seronegative on Day 28 after farrowing, indicating that the transmission of ADV had been blocked during the embryo transfer. Untreated control embryos transferred into AD-free recipients caused seroconversion based on the serum neutralization test carried out 40 d later.

In two sets of experiments eight groups of seven-week-old pigs weighing about 9 kg were fed for three weeks a prestarter that contained 0.5, 1.0, 2.0, 3.0, 4.0, 5.0, 10.0 or 15.0 mg/kg of highly purified T-2 toxin. The feed of the two control groups was free from T-2 toxin. Average daily intakes of toxin by the pigs were 0.38, 0.81, 1.24, 1.43, 0.93, 0.81, 0.99 and 2.5 mg, respectively. The weight gains, the feed intakes, the extent of feed refusal, the parameters of energy and protein metabolism and the serum concentrations of calcium, inorganic phosphorus and magnesium were affected to different extents by the different doses of T-2 toxin, but the data indicated that feed consumption was reduced and the activity of aspartate aminotransferase was increased by the smallest amount of T-2 toxin tested.

Four groups of seven-week-old pigs weighing about 9 kg were fed for three weeks a prestarter that contained 0.5, 1.0, 2.0 or 3.0 mg/kg of highly purified T-2 toxin. The average daily intakes of toxin by the pigs were 0.38, 0.81, 1.24 and 1.43 mg, respectively. The experimental and control pigs were immunised with 5 ml aluminum hydroxide gel-absorbed purified horse globulin on the first and fourth days of the treatment period. Blood samples were withdrawn on days 7, 14 and 21 and used for the determination of the titre of anti-horse globulin antibody, for an in vitro lymphocyte proliferation test, using purified horse globulin, phytohaemagglutinin and concanavalin-A and for determinations of the immune complex, the cytotoxic reaction and the phagocytic activity and phagocytic index of circulating granulocytes. The samples taken on day 21 were also used to determine the erythrocyte count, the mean cell volume of the erythrocytes, the haematocrit, the blood haemoglobin concentration, the leucocyte count and the proportion of T lymphocytes. At the end of the experiment samples were taken from the thymus, spleen and mesenteric lymph nodes for histological examination. The diets that contained 2 and 3 mg T-2 toxin/kg caused a significant decrease in the red blood cell count, the mean corpuscular volume and the haemoglobin concentration. A significant decrease in the leucocyte count and the proportion of T lymphocytes was observed in all the treatment groups. There were also dose-dependent, significant decreases in antibody formation and in the blastogenic transformation of lymphocytes, and mild to moderate reactive processes were observed histologically in the lymphoid organs.