The Cretaceous-Paleogene (KPg) boundary is demarked by two critical and intimately linked events: a global-scale impact caused by a massive extraterrestrial body, and a resultant catastrophic planet-wide mass-extinction, with pivotal long-term consequences for life on Earth. The site of impact has been identified as the ~180 km wide Chicxulub crater complex [Yucatan peninsula, Mexico] based on strong geochemical, sedimentological, and temporal evidence. However, the impactor, which was obliterated on contact during the cratering process, has eluded identification. Previous studies struggled to identify the projectile based on scant geochemical and isotopic traces admixed with the ejecta and melt-rock, plus a single heavily altered microscopic fragment possibly from the impactor. While those efforts helped to rule out some potential sources and narrowed down the best potential candidates to a CM, CR, or CO carbonaceous chondrite, the data was insufficient to provide an incontrovertible diagnosis, and the identity of the Chicxulub projectile remained a critical missing detail. Here we describe new, exceptionally-preserved fragments of cosmic origin that were directly associated with Chicxulub impact ejecta from a temporally constrained KPg boundary site in North Dakota (U.S.A.). The fragments occur as inclusions within unaltered glassy ejecta spherules, which likely protected them against chemical and physical degradation, leading to their marked preservation. The spherules themselves were also uniquely preserved in amber, which inhibited their usual breakdown to smectitic clay. Geochemical examination of the inclusions were undertaken using electron microprobe, laser-ablation inductively-coupled-plasma-mass-spectroscopy, and synchrotron X-ray techniques. Results provide multiple lines of evidence that support a cosmic origin and chemistry indicative of a CM subtype carbonaceous chondrite, offering new support of prior hypotheses. The data is also in agreement with projectile residues from the Chicxulub impact fallout. In addition to its utility in reconstructing the dynamics of the Chicxulub event, this data helps provide extra dimension to our understanding of major impact processes and their effects on Earth.

<p>Dinosaurs and pterosaurs, critical components of late Mesozoic terrestrial biomes, have thus far never been reported in-situ from deposits immediately below the Cretaceous-Paleogene (KPg) boundary. Strata from the KPg boundary preserve worldwide evidence of a massive cosmic impact that resulted in grave consequences for Earth’s biota, triggering a rapid global mass extinction that ultimately claimed ~75% of species. While trends in long-term biotic effects are well-documented, little is known about the fate of biota coeval with the impact because well-preserved fossil evidence is lacking for that brief time interval. Here we report the first-known occurrences of in-situ dinosaur and pterosaur remains coincident with the KPg boundary, providing a vantage point that is closer in temporal proximity to the Chicxulub impact than any prior known records for these clades. The fossils, preserved in a Chicxulub impact-triggered surge deposit and mass-death assemblage in the Hell Creek Formation, U.S.A., consist of a well-developed semi-articulated prenatal pterosaur in ovum and partially articulated remains of a subadult ornithischian dinosaur (Family Thescelosauridae). Examination via synchrotron rapid scanning X-ray fluorescence (SRS-XRF), scanning electron microscopy (SEM), and traditional light microscopy revealed extensive preservation of soft tissue consisting of distinct signatures of organic residues as well as three-dimensional structure. The pterosaur embryo, which comprises the first known from the Late Cretaceous and the only example thus far from North America, contributes information pivotal to understanding the prenatal development and early ontogeny of these animals from a time interval for which no such data exists. The ornithischian (c.f. <em>Thescelosaurus</em> sp.) dinosaur, which includes a remarkably complete articulated hind limb enclosed within three-dimensional lithified tubercular skin envelope, provides important data regarding the osseous and soft-tissue anatomy of the Thescelosauridae. In addition to contributing invaluable new anatomical and physiological insights, these specimens constitute the first conclusive evidence of the direct participation of pterosaurs and dinosaurs in the terminal-Cretaceous extinction event.</p>

The end-Cretaceous Chicxulub impact triggered Earth's last mass-extinction, extinguishing ~ 75% of species diversity and facilitating a global ecological shift to mammal-dominated biomes. Temporal details of the impact event on a fine scale (hour-to-day), important to understanding the early trajectory of mass-extinction, have largely eluded previous studies. This study employs histological and histo-isotopic analyses of fossil fish that were coeval with a unique impact-triggered mass-death assemblage from the Cretaceous-Paleogene (KPg) boundary in North Dakota (USA). Patterns of growth history, including periodicity of ẟ18O and ẟ13C and growth band morphology, plus corroborating data from fish ontogeny and seasonal insect behavior, reveal that the impact occurred during boreal Spring/Summer, shortly after the spawning season for fish and most continental taxa. The severity and taxonomic symmetry of response to global natural hazards are influenced by the season during which they occur, suggesting that post-impact perturbations could have exerted a selective force that was exacerbated by seasonal timing. Data from this study can also provide vital hindsight into patterns of extant biotic response to global-scale hazards that are relevant to both current and future biomes.

Ancient biomolecule analyses are proving increasingly useful in the study of evolutionary patterns, including extinct organisms. Proteomic sequencing techniques complement genomic approaches, having the potential to examine lineages further back in time than achievable using ancient DNA, given the less stringent preservation requirements. In this study, we demonstrate the ability to use collagen sequence analyses via proteomics to assist species delimitation as a foundation for informing evolutionary patterns. We uncover biogeographic information of an enigmatic and recently extinct lineage of Nesophontes across their range on the Caribbean islands. First, evolutionary relationships reconstructed from collagen sequences reaffirm the affinity of Nesophontes and Solenodon as sister taxa within Solenodonota. This relationship helps lay the foundation for testing geographical isolation hypotheses across islands within the Greater Antilles, including movement from Cuba toward Hispaniola. Second, our results are consistent with Cuba having just two species of Nesophontes (N. micrus and N. major) that exhibit intrapopulation morphological variation. Finally, analysis of the recently described species from the Cayman Islands (N. hemicingulus) indicates that it is a closer relative to N. major rather than N. micrus as previously speculated. This proteomic sequencing improves our understanding of the origin, evolution, and distribution of this extinct mammal lineage, particularly with respect to the approximate timing of speciation. Such knowledge is vital for this biodiversity hotspot, where the magnitude of recent extinctions may obscure true estimates of species richness in the past.

Myriapods were, together with arachnids, the earliest animals to occupy terrestrial ecosystems, by at least the Silurian. The origin of myriapods and their land colonization have long remained puzzling until euthycarcinoids, an extinct group of aquatic arthropods considered amphibious, were shown to be stem-group myriapods, extending the lineage to the Cambrian and evidencing a marine-to-terrestrial transition. Although possible respiratory structures comparable to the air-breathing tracheal system of myriapods are visible in several euthycarcinoids, little is known about the mechanism by which they respired. Here, we describe a new euthycarcinoid from Upper Devonian alluvio-lagoonal deposits of Belgium. Synchrotron-based elemental X-ray analyses were used to extract all available information from the only known specimen. Sulfur X-ray fluorescence (XRF) mapping and spectroscopy unveil sulfate evaporation stains, spread over the entire slab, suggestive of a very shallow-water to the terrestrial environment prior to burial consistent with an amphibious lifestyle. Trace metal XRF mapping reveals a pair of ventral spherical cavities or chambers on the second post-abdominal segment that do not compare to any known feature in aquatic arthropods, but might well play a part in air-breathing. Our data provide additional support for amphibious lifestyle in euthycarcinoids and show that different respiratory strategies were used during the marine-to-terrestrial transition in the myriapod lineage.

Cayman Brac (Cayman Islands) lies within the Caribbean Islands Biodiversity Hotspot, an epicenter of high biodiversity and endemism. However, all endemic terrestrial mammals on the Cayman Islands are now extinct, following post-1500 AD human colonization of the islands. Introduced rodents and domesticated mammals now exclusively represent this facet of terrestrial fauna on the Cayman Islands, and are a likely cause of endemic species loss on the islands. Cayman Brac has numerous caves and rock fissures that offer protection to a naturally accumulated ensemble of vertebrate sub-fossil bone remains, documenting modifications in island biodiversity through the Holocene. In this study, we showcase the first molecular faunal survey undertaken on sub-fossil remains from the Cayman Islands, using collagen fingerprinting for taxonomic identification of the cave skeletal deposits collected from a single cave system, Green Cave on Cayman Brac. Collagen type (I) extracts from 485 bone fragments were analyzed to determine faunal identity and assemblage composition. A total of 76% of the collagen fingerprint-yielding samples were mammalian in origin, 67% of which were identified as invasive murid rodents. Here, we present mass spectral biomarkers for the endemic terrestrial mammal fauna of Cayman Brac, including the extinct capromyid rodents, Capromys and Geocapromys (Rodentia: Capromyidae), alongside commentary on the composition of the sub-fossil bone assemblage between the five distinct depositional chambers that comprise Green Cave. Collagen (I) provides a key service in taxonomic identification and mapping of macroevolutionary trends, and these results suggest a pivotal role for murid rodents in the competition and extinction of terrestrial endemic mammals from the Cayman Islands.

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Ancient stromatolites can provide key insights into the early evolution of life on Earth. Neoarchean fenestrate stromatolites from the similar to 2520 Ma Upper Nauga Formation (Transvaal Supergroup, South Africa) preserve cuspate morphologies. They possess clearly delineated support and drape structures interpreted as dolomitized microbial mat material. Petrographic observations show that the biogenic structures are composed of planar-s to non-planar ferroan dolomite, encased in ferroan calcite, including herringbone calcite textures. The cuspate stromatolites were analyzed using Synchrotron Rapid Scanning X-Ray Fluorescence (SRS-XRF) and more conventional techniques to determine: (i) whether element distributions could be distinguished in ancient stromatolites at both cm to dm scales, (ii) whether element distributions show variation between biogenic and abiogenic textures, and (iii) the sample's paragenesis. The distributions of Ca, Fe, Mn, Pb, Cu, As, Br, Al, Si, P, and S directly correspond to dolomitized stromatolitic structures and show trace element distributions are principally controlled by calcite and dolomite occurrence. Dolomite formation was mainly driven by seawater-derived fluids given the high concentrations of Fe and retention of marine shale-normalized rare earth element and yttrium (REYSN) patterns, however the spatial association of dolomite to stromatolite structures may reflect microbially-influenced mineral nucleation. Given the complexity of this sample's paragenetic evolution, trace metal distributions cannot be conclusively tied to specific metabolic pathways, bioaccumulation or passive binding, however, the results show SRS-XRF can be used for quantifiable, spatial, in-situ investigation of ancient microbialites. (C) 2019 Elsevier Ltd. All rights reserved.

Recent progress has been made in paleontology with respect to resolving pigmentation in fossil material. Morphological identification of fossilized melanosomes has been one approach, while a second methodology using chemical imaging and spectroscopy has also provided critical information particularly concerning eumelanin (black pigment) residue. In this work we develop the chemical imaging methodology to show that organosulfur-Zn complexes are indicators of pheomelanin (red pigment) in extant and fossil soft tissue and that the mapping of these residual biochemical compounds can be used to restore melanin pigment distribution in a 3 million year old extinct mammal species (Apodemus atavus). Synchotron Rapid Scanning X-ray Fluorescence imaging showed that the distributions of Zn and organic S are correlated within this fossil fur just as in pheomelanin-rich modern integument. Furthermore, Zn coordination chemistry within this fossil fur is closely comparable to that determined from pheomelanin-rich fur and hair standards. The non-destructive methods presented here provide a protocol for detecting residual pheomelanin in precious specimens.

Our study is the first to correlate differential distributions of trace elements within the different tissue types of zonal bone.

Endochondral ossification is the process by which bone is deposited during development, growth and repair of the skeleton.