目的 探讨PI-PLCβ1的表达水平在骨髓增生异常综合征(myelodysplastic syndromes,MDS)发病过程中的意义.方法 采用骨髓形态学和细胞遗传学分析,选取初诊确诊的典型MDS 30例,复诊确诊的不典型MDS 10例;用荧光定量PCR的方法检测40例MDS和10例正常骨髓中PI-PLCβ1的表达水平.结果 初诊确诊MDS和复诊确诊MDS的PI-PLCβ1表达水平低于正常对照组(P＜0.05);不同分型的MDS中,高危组PI-PLCβ1表达水平低于低危组(P＜0.05).结论 在早期MDS的诊断及MDS的临床预后中,检测PI-PLCβ1的表达水平有重要的应用价值.

目的 探讨骨髓巨核细胞与外周血血小板参数变化在免疫性血小板减少症(ITP)鉴别诊断中的应用价值.方法 研究设ITP患者组(50例)、非ITP患者组(47例)和健康人对照组(45例),分别检测ITP和非ITP组患者骨髓中巨核细胞及外周血血小板参数及健康对照组外周血血小板参数;采用t检测进行统计学分析.结果 与非ITP组相比,ITP组患者骨髓中巨核细胞数量明显增加,其中幼稚巨、颗粒型巨核细胞明显增加,产板型巨核细胞明显减少,差异有统计学意义(P＜0.01),而外周血血小板各参数两组间无差异(P＞0.05).ITP组和健康对照组相比,血小板数量和压积均明显降低,有统计学意义(P＜0.01).结论 骨髓形态学及外周血小板相关参数,对ITP的诊断和鉴别诊断具有重要的参考价值.

Objective To investigate the significance of C-reactive protein(CRP) expression in patients with diffuse large B lymphoma(DLBCL) and its relationship with prognosis.Methods 91 cases of DLBCL patients in our hospital were selected, and the healthy subjects were selected in the same period of time.The concentrations of CRP in all subjects were measured by enzyme-linked immunosorbent assay(ELISA) in 87 cases.Results The expression level of CRP in patients was DLBCL(24.03 + 6.92) mg/L.Compared to healthy people(6.59 + 2.87) mg/L, it increased significantly.When compared the control group and the different pathological stages of CRP levels, there was a statistically significant difference(P<0.05), and with the level increased with clinical stage increased.The high expression rate in patients with CRP chemotherapy was 44.83%, and the low expression of CRP group was 77.27%.There were significant differences between groups χ2=31.728, P<0.05.The serum CRP level in control group were lower than that of GCB group and NGCB group, while the GCB group was lower than in NGCB group, and the difference between groups was statistically significant(P<0.05).Conclusion CRP detection can effectively reflect the different types, stages and severity of disease in patients with DLBCL disease which can help to estimate the disease changes and prognosis.

The present study aimed to investigate the regulatory mechanism of the AmpC enzyme by analyzing the construction and function of AmpCR, AmpE and AmpG genes in the Dhahran (DHA)‑1 plasmid of Klebsiella pneumoniae (K. pneumoniae). The production of AmpC and extended‑spectrum β‑lactamase (ESBL) were determined following the cefoxitin (FOX) inducing test for AmpC, preliminary screening and confirmation tests for ESBL in 10 DHA‑1 plasmid AmpC enzymes of K. pneumoniae strains. AmpCR, AmpD, AmpE and AmpG sequences were analyzed by polymerase chain reaction. The pACYC184‑X plasmid analysis system was established and examined by regulating the pAmpC enzyme expression. The electrophoretic bands of AmpCR, AmpD, AmpE and AmpG were expressed. Numerous mutations in AmpC + AmpR (AmpCR) and in the intergenic region cistron of AmpC‑AmpR, AmpD, AmpE and AmpG were observed. The homology of AmpC and AmpR, in relation to the Morganella morganii strain, was 99%, which was determined by comparing the gene sequences of Kp1 with those of Kp17 AmpCR. The specific combination of AmpR and labeled probe demonstrated a band retarded phenomenon and established a spatial model of AmpR. All the enzyme production strains demonstrated Val93→Ala in AmpG; six transmembrane domains were found in AmpE in all strains, with the exception of Kp1 and Kp4, which had only three transmembrane segments that were caused by mutation. The DHA‑1 plasmid AmpC enzymes encoded by plasmid are similar to the inducible chromosomal AmpC enzymes, which are also regulated by AmpD, AmpE, AmpR and AmpG.

多发性骨髓瘤（multiple myeloma,MM）是以浆细胞异常增生为特征的一种恶性肿瘤疾病,占血液系统肿瘤的10%、全身恶性肿瘤的1%。好发于中老年人,近年来由于我国人口老龄化及人们对该病的认识水平提高,该病发病率有逐渐增加趋势。染色体异常是MM预后的重要指标,而常规染色体

Objective To investigate the molecular biology of one cefoxitin-resistant Klebsiella pneumoniae strain.Methods AmpC and AmpR genes of one cefoxitin-resistant Klebsiella pneumoniae were analyzed by PCR,and then the pET22b(+)-AmpR expression plasmid and expression strain were constructed.Results A 1 140 bp segment of AmpC and a 876 bp segment of AmpR were cloned.These sequences showed that recombinant AmpC and AmpR genes were highly identical to those of Morganella morganii.Fusion protein about 34 kD was expressed by E.coli BL21(DE3) transfected with recombinant plasmid pET22b(+)-AmpR.Conclusion There are regulator AmpR genes in plasmid of cefoxitin-resistant Klebsiella pneumoniae isolates.AmpR genes express AmpR fusion protein.Induced resistance mechanism is also found in plasmid-mediated AmpC β-lactamase.

AmpC酶,又称头孢菌素酶,Bush分类属于Group-1型,Amber分类属Class C类β-内酰胺酶,能水解三代头孢菌素、头霉素类,不能被酶抑制剂克拉维酸、舒巴坦和他唑巴坦抑制.目前AmpC酶表达的调控机制尚不完全清晰,下面就其调控基因之一ampR的研究现状进行综述.