目的:分析富血小板血浆(PRP)肌筋膜内注射治疗带状疱疹后神经痛(PHN)的疗效.方法:选取2019年1月至2021年12月收治的60例老年胸腰段PHN患者,所有患者均接受肌筋膜内注射PRP治疗.记录所有患者的治疗疗效和不良反应发生情况,同时比较治疗前后的疼痛数字评分(NRS)、匹兹堡睡眠质量指数(PSQI)、焦虑自评量表(SAS)及抑郁自评量表(SDS)等量表评分,以及血清疼痛介质水平.结果:所有患者的治疗总有效率为93.33％,不良反应总发生率为18.33％.随访3个月,治疗后1个月和3个月,所有患者的NRS和PSQI评分均明显低于治疗前,差异有统计学意义(P＜0.05);治疗后3个月,所有患者的SAS和SDS评分均明显低于治疗前,差异有统计学意义(P＜0.05),血清β-EP、NT水平均明显高于治疗前,差异有统计学意义(P＜0.05),血清CGRP、VGF等水平均明显低于治疗前,差异有统计学意义(P＜0.05).结论:PRP肌筋膜内注射治疗PHN患者疗效显著,能明显减轻疼痛程度,提升睡眠质量,改善患者情绪状态,且治疗安全性高,值得临床推广应用.

目的:探讨盆底三维超声对健康初产女性不同分娩方式盆膈裂孔的应用价值.方法:以2017年1月至2018年1月为时间段,选取我院收治的健康初产妇60例作为本次对象进行研究,回顾性分析其相关临床资料,根据不同分娩方式将其分为对照组(剖宫产,30例)和观察组(阴道分娩,30例).两组均对盆膈裂孔大小、形态等采用盆底三维超声进行观察,并测量其相关参数包括盆膈裂孔的面积、周长、前后径、左右径等,对正中矢状切面膀胱颈移动度(BND)在二维灰阶模式下完成测量.结果:在VALsalva状态下,两组产妇正中矢状切面BND、盆膈裂孔的面积、周长、前后径、左右径等对比存在统计学差异(P＜0.05).在静息状态下,两组盆膈裂孔相关支持组织功能数据对比无统计学差异(P＞0.05).结论:盆底三维超声对健康初产女性不同分娩方式盆膈裂孔的诊断效果显著,即可清晰、直观、准确的检查盆膈裂孔,且具有性价比高、可重复性强、方便快捷等优势,对诊断产后盆地功能障碍性疾病以及提供康复治疗依据具有积极意义,值得应用推广.

目的 探究血清 β-hCG、CRP和Npt水平在胎膜早破孕妇羊膜腔感染诊断中的应用价值.方法 选取胎膜早破孕妇100例,根据其是否存在羊膜腔感染的情况分为2组,对比这2组孕妇血清 β-hCG、CRP和Npt水平的差异性.结果 实验组血清 β-hCG、CRP和Npt水平均明显高于对照组(P<0.05).结论 血清 β-hCG、CRP和Npt水平可以作为胎膜早破孕妇是否存在羊膜腔感染的预测指标.

Objective To study the immune chromatography rapid method to detect the intestinal tract and application effect of adenovirus and rotavirus.Methods Select our hospital 256 cases of diarrhea of infant patients as the research object, the application of rapid method to detect the patient's immune chromatography intestinal adenovirus and rotavirus&nbsp;antigen, analysis of different seasons, children under the age of the intestinal tract of rotavirus and adenovirus positive rate of detection.Results256 cases, intestinal adenovirus positive rate was 1.6%, positive rate was 23.0% of rotavirus, compare the difference was statistically significant(P<0.05); Summer and autumn of adenovirus detection rate of 0.0%; Rotavirus all have checked out all the year round, winter has the highest detection rate. Age distribution, the positive rate of intestinal adenovirus and rotavirus detection with the increase of the age gradually reduce; 0 to 1 years old,1 to 2 years old, 2 to 3 years old,> 3 years old children with rotavirus and adenovirus detection of positive contrast difference had statistical signiifcance (P<0.05);The rate of rotavirus children aged 0 to 1.Conclusion The immune chromatography rapid method to detect the intestinal tract of rotavirus and adenovirus application effect is satisifed.

Objective To infantile viral diarrhea rotavirus and intestinal adenovirus infections epidemiological analysis.MethodsIn our hospital, clinical data of viral diarrhea in infants were retrospectively analyzed, using PCR reaction to test the intestinal adenovirus, using enzyme-linked immunosorbent assay of rotavirus, and the positive specimens are G, P.Results 150 cases of infants and young children treated, rotavirus positive detection rate was 47.33%, the intestinal glands virus positive detection rate of 11.33%.Conclusion Rotavirus is the major pathogen in infantile viral diarrhea, intestinal adenovirus is also an important pathogen in children with diarrhea, should strengthen the study of these two kinds of virus detection, key protection under two years old infants and young children.

The application of many nucleoside analogs has produced multidrug-resistant mutant strains of hepatitis B virus (HBV). This study aimed to analyze the association between HBV gene mutations in chronic hepatitis B patients and antiviral multidrug resistance. The whole HBV genome in serum samples of six cases of clinical multidrug-resistant patients was amplified using polymerase chain reaction (PCR), then purified and cloned into the pMD18T plasmid to construct 19 clones of recombinant pMD18T-HBV plasmids for whole genome sequencing. The mutations in the P region and other regions in the HBV genome were analyzed. We found that the 19 clones of HBV from these six cases were all of the C genotype. The major common mutation site in the P region was rtM204V/I, and the accompanied common sites were rtQ333K, rtH337N, and rtD392S. The common mutations in the S region of the 19 clones were T56N, K57Q, D62A, and V157A. The common mutation sites in the X region were A41S, G85A, and L92V. There was no common mutation site discovered in the C region. In conclusion, the detection of mutation sites in HBV will help reveal the mechanism underlying the multidrug-resistance of HBV and improve clinical antiviral therapy in chronic hepatitis B patients.

目的 调研深圳市盐田区实施孕早期出生缺陷筛查项目前后出生缺陷发生率及产前确诊情况,为完善盐田区出生缺陷干预方案提供依据.方法 对盐田区1 954名孕妇在孕早期(孕周≤13周)开展出生缺陷检查,并风险评估和咨询指导.结果 发现11例严重先天残疾胎儿,给予治疗性引产;早孕建卡率上升9.50%,孕产妇系统管理率上升11.04%;出生缺陷率下降到10.28‰,比项目实施前下降3.54‰.结论 孕早期出生缺陷干预是防止严重出生缺陷儿出生的重要措施,政府主导、综合干预可有效降低出生缺陷儿的发生.

目的 观察和分析血清β-人绒毛膜促性腺激素(β-hCG)、C-反应蛋白(CRP)和新嘌呤(Npt)水平与足月胎膜早破(PROM)孕妇合并羊膜感染的相关性.方法 选取150例足月PROM孕妇作为研究对象,根据是否存在羊膜感染将纳入的孕妇分为感染组57例和非感染组93例.对两组孕妇产前的血清β-hCG、CRP、Npt水平进行检测和比较.结果 感染组患者的血清β3-hCG、CRP、Npt水平均高于非感染组,两组之间的差异均有统计学意义(t=4.128、6.713、3.925,均P＜0.05);应用上述三种血清指标联合检测诊断足月PROM合并羊膜感染的敏感度均显著高于单独应用,差异有统计学意义(x2=12.755、11.528、9.205,均P＜0.05),而应用三种血清指标联合和单独应用一种指标检测诊断足月PROM合并羊膜感染的特异度差异无统计学意义(x2=1.632,P＞0.05);感染组孕妇的血清β-hCG水平与其血清CRP水平和Npt水平呈正相关关系(r=0.516、0.463,P＜0.05),其血清CRP水平与血清Npt水平呈正相关关系(r=0.582,P＜0.05).结论 足月PROM合并羊膜感染孕妇表现为血清β-hCG、CRP、Npt水平的显著升高,上述三种血清指标具有良好的相关性,而且进行联合检测时可提高诊断的敏感度,可作为诊断的辅助指标.

Cetyl trimethyl ammonium bromide/sodium dodecyl benzene sulfonate (CTAB/SDBS) complex formulation of scale effect on the spontaneous formation of vesicles and the influence of different factors on the stability of vesicles were discussed, structure and morphology of vesicles were observed.

Recent studies have shown that the Moloney leukemia virus 10 (Mov10), a putative RNA helicase, has very broad and potent antiretroviral activities. Hepatitis B virus (HBV) has a reverse transcription process, but the potential role of Mov10 in HBV replication remains unknown. In this study, Mov10 was demonstrated to affect HBV expression in HepG2 and HepG2.2.15 cell lines. The data showed that the over-expression of exogenous Mov10 resulted in an increase of the HBsAg and HBeAg levels in the culture supernatant and HBV mRNA level in transfected cells at a low dose and resulted in a decrease at a high dose, but HBV DNA in culture supernatant was not affected. The knockdown of endogenous Mov10 expression through siRNA treatment could suppress levels of HBsAg, HBeAg and HBV mRNA, but had no effect on HBV DNA. Above results indicate that an appropriate level of exogenous Mov10 is responsible for HBV replication, that any perturbation in the level of Mov10 could affect HBV replication, while the endogenous Mov10 could promote HBV replication in vitro. The precise mechanisms that underlie the action of Mov10 on HBV still need further investigation.

The treatment of infection with lamivudine-resistant mutants of hepatitis B virus (HBV) with mutations in the YMDD motif has become a crucial issue in the clinic. In this work, the plasmids pcDNA3.1 (+)-HBV/C-YVDD and pcDNA3.1 (+)-HBV/C-YMDD were constructed and injected into BALB/c mice using a hydrodynamics-based procedure to investigate viral replication and expression of HBV lamivudine-resistant YVDD mutants in vivo. Compared with the YMDD group, HBsAg levels were higher in sera of mice in the YVDD group, but HBeAg levels were lower on day 1 after injection. Levels of HBcAg in hepatocytes were higher in the YVDD group on day 1, whereas the HBsAg levels were lower. The levels of HBV mRNA in the liver were higher in mice in the YVDD group on day 1 after injection. The results showed that injection with these plasmids resulted in efficient initiation of replication of HBV in mice and also suggested that the combined mutations in YVDD mutants could affect the replication process.

目的探讨超声诊断胎儿脐带绕颈的价值及对胎儿宫内窘迫的预测,及其对选择分娩方式的指导意义。方法对320例经超声诊断的晚期妊娠胎儿脐带绕颈病例进行观察分析,了解胎儿宫内窘迫的情况,并与分娩证实的结果进行对比分析研究。结果 320例超声诊断的胎儿脐带绕颈病例中,产后证实者312例,假阳性率为2.5%。结论应用超声尤其是彩色多普勒血流成像(CDFI)检测胎儿脐带绕颈快捷准确,通过对脐动脉血流参数的测定、胎心率的观察等等还可早期诊断胎儿宫内窘迫,对临床分娩方式和时机的选择,降低围生儿并发症有着极大的临床指导意义。

手术治疗成人长骨干骨折,选择合适的内固定器械是治疗成功与否的关键.锁定加压钢板小切口治疗成人长骨干骨折具有切口小、创伤小;固定牢固;术后早期活动、并发症少;骨折愈合率高等优点,是目前长骨干骨折内固定术中比较理想的方法之一.我院于2007年3月～2009年12月应用锁定加压钢板小切口治疗各种类型的长骨干骨折57例,疗效满意,现报告如下.

股骨转子间骨折是老年人常见骨折,虽然治疗股骨转子间骨折的内固定器材较多,但由于老年人骨质疏松,仍有较多失败病例.选择合适的内固定器械是治疗成功与否的关键.股骨近端锁定钢板治疗股骨转子间骨折具有固定牢固,术后早期活动,并发症少,骨折愈合率高等优点,是目前股骨转子间骨折内固定术中比较理想的方法之一.我院于2008年3月～2009年12月应用股骨近端锁定钢板治疗各种类型的股骨转子间骨折38例,疗效满意,现报告如下.

目的探讨腺苷脱氨酶(ADA)测定在结核性脑膜炎的诊断价值和意义。方法选择38例结核性脑膜炎患者作为结核组,选择40例非结核性脑膜炎患者作为非结核组,以ADA>8U/L作为阳性阈值,比较两组患者脑脊液ADA活性及阳性率测定结果。结果结核组脑脊液ADA活性和阳性率,明显高于非结核组,差异有显著性(P<0.01)。结论ADA测定对结核性脑膜炎的诊断与鉴别诊断具有很高的价值,值得推广。

手术治疗成人长骨干骨折,选择合适的内固定器械是治疗成功与否的关键.记忆合金环抱器治疗长骨干骨折具有固定牢固、可术后早期活动、并发症少、骨折愈合率高等优点.2004年3月～2007年1月,我院应用记忆合金环抱器治疗各种类型的长骨干骨折38例,效果满意,现报告如下.

目的:探讨评价超声对子宫内膜息肉的临床诊断价值。方法:应用经腹及经阴道超声对60例患者进行子宫内膜息肉的诊断,并与宫腔镜、手术、病理结果对照分析。结果:诊断与病理结果符合54例(90%),误诊3例(5%),漏诊3例(5%)。结论:超声对子宫内膜息肉诊断符合率高,对于临床治疗方面具有极其重要的价值。

目的探讨血清胱抑素c(cystatin c)在儿童肾病早期诊断中的临床意义方法采用乳胶颗粒增强免疫比浊法(TPNIA)测定62例儿童肾病患者血清胱抑素c,同时测定血肌酐(SCr,酶法)及肌酐清除率(CCr),比较cystatin c,SCr与CCr的相关性,其中以cystatinc与CCr的相关程度最为密切。结论cystatin c在儿童肾病早期诊断中具有较高的准确性。能可靠的反映肾功能早期损害的指标。在儿童肾病的早期诊断中具有指导意义。