Extradural compression of the spinal cord is the most frequent neurological complication of malignant lymphomas (10). It also occurs in other hematoblastomas and can cause a rapidly progressive neurological deficit. Although these tumors are mostly radiosensitive (8, 9) and chemotherapy has made remarkable progress (2, 6, 7, 10, 13), surgical excision often remains absolutely necessary. Probably nowhere is palliation more justified than in malignant spinal cord compression, in order to relieve intolerable pain and to avert the catastrophic consequence of threatening paraplegia (12).

Eine Nachuntersuchungsserie von 446 Patienten, die wegen lumbaler Bandscheibenhernien operiert worden waren, zeigte ein gutes bzw. sehr gutes Befinden in 399 Fällen. Mangelhaft war das Ergebnis bei 42, schlecht war das Ergebnis bei 5 Patienten. Männer zeigten im Vergleich zu Frauen eine etwas bessere Restitution. Die Erfolge bei Schwerarbeitern unterschieden sich nur unwesentlich von den Erfolgen anderer Berufsgruppen. Übergewichtige und Untergewichtige zeigten ebenfalls nahezu idente Resultate. Bezogen auf das Lebensalter ließ sich ein etwas günstigeres Ergebnis bei älteren Patienten erkennen.

The aim of this work was to study the pathway(s) of sugar phosphate metabolism in chloroplasts of the unicellular green alga, Dunaliella marina (Volvocales). Phosphofructokinase, detectable in crude cell extracts, copurifled with intact chloroplasts on sucrose density gradients. In isolated chloroplasts, phosphofructokinase activity displayed latency to the same degree as chloroplast marker enzymes. From the quantitative distribution of enzyme activities in fractionated cells, it is concluded that there is an exclusive localization of phosphofructokinase in chloroplasts. In addition, no separation into multiple forms could be achieved. For the study of regulatory properties, chloroplast phosphofructokinase was partially purified by ammonium sulfate fractionation followed by DEAE-cellulose chromatography. The pH optimum of the enzyme activity was 7.0 and was not altered with varying concentrations of substrates or low-molecular-weight effectors. Fructose 6-phosphate showed a sigmoidal saturation curve whose shape was further changed with varying protein concentrations of the preparation. The second substrate, ATP, gave a hyperbolic saturation curve with a Michaelis constant of 60 μm. At a Mg2+ concentration of 2.5 mm, ATP concentrations exceeding 1 mm inhibited the enzyme in a positive cooperative manner. The same type of inhibition was observed with other phosphorylated intermediates of carbon metabolism, the most efficient being phosphoenolpyruvate, glycolate 2-phosphate, glycerate 3-phosphate, and glycerate 2-phosphate. Inorganic phosphate was the only activator found for phosphofructokinase. With nonsaturating fructose 6-phosphate concentrations, Pi activated in a positive cooperative fashion, while no activation occurred with saturating fructose 6-phosphate concentrations. In the presence of either an activator or an inhibitor, the sigmoidal shape of the fructose 6-phosphate saturation curve was altered. Most notably, the activator Pi could relieve the inhibitory action of ATP, phosphoenolpyruvate, glycerate 3-phosphate, glycerate 2-phosphate, and glycolate 2-phosphate. Based on these experimental findings, the regulatory properties of D. marina chloroplast phosphofructokinase are discussed with respect to its playing a key role in the regulation of chloroplast starch metabolism during a light/dark transition. All available evidence is compatible with the interpretation that phosphofructokinase is active only in the dark thus channeling starch degradation products into glycolysis.

Prämature Harnblasen-Detrusor-Kontraktionen bei einer supranukleären Läsion werden als neurogen enthemmte Harnblase, ohne neurologisch lokalisierbare Läsion als unstabile Harnblase bezeichnet. Diese Harnblasenhyperaktivität äußert sich klinisch als (1) imperativer Harndrang mit/ohne Einnässen, (2) Pollakisurie, (3) Nykturie mit/ohne Enuresis.

A protein inhibiting salivary and pancreatic a-amylase of mammalian origin is contained in dry seeds of beans (Phaseolus vulgaris). Starting from a crude bean extract, the amylase inhibitor may be purified about 30fold in one step to apparent homogeneity by chromatography on matrix-bound salivary amylase. Compared with protein obtained by a conventional purification procedure and in similar yield, the amylase inhibitor obtained by affinity chromatography had the same specific activity (4.5 (akat inhibitor units/mg protein). A one step purification from crude extracts to homogenous inhibitor with the same specific activity was achieved by immuno-affinity chromatography on immobilized rabbit antibody raised against pure amylase inhibitor. The yield was 60 % that of a conventional purification. Criteria of purity of the inhibitor protein were thin-layer electrofocussing and immuno-electrophoresis.

Abstract The photoautotrophic procaryote, Anacystis nidulans, accumulates glycogen as a carbon reserve during illumination and nitrogen limitation. Glycogen phosphorylase participates in the mobilization of the polysaccharide in a dark period and was found to be a point of regulatory control of glycogen degradation. Specific enzyme activity of non‐dividing cells increased twofold upon transfer from light to dark and decreased again upon re‐illumination. This dark stimulation of enzyme activity was not inhibited by either rifampicin or chloramphenicol, whereas a light‐induced decrease was abolished by DCMU, an inhibitor of photosynthetic electron transport. Deactivation of glycogen phosphorylase could be simulated by dithiothreitol in vitro. A spheroplast lysate either with or without chromatophores and a partially purified glycogen‐enzyme complex responded to dithiothreitol.These results are compatible with an interpretation in terms of redox changes taking place with the enzyme in an activation/deactivation cycle. The inactive form maintained in a reduced state by photosynthetic electron transport is thought to be converted into an active form in the dark.

The organism Bacillus amyloliquefaciens is capable of producing alpha-amylase (1,4-alpha-D-glucan glucanohydrolase, EC 3.2.1.1) and isoamylase (glycogen 6-glucanohydrolase, EC 3.2.1.68) extracellurlarly and a membrane-bound, intracellular alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20). The amounts of alpha-glucosidase in cells of B. amyloliquefaciens grown on amylaceous polysaccharides were significantly higher then in cells grown on non-carbohydrate carbon sources. alpha-Glucosidase was exclusively found associated with membranes from ruptured spheroplasts by subcellular fractionation and solubilization studies. Salt solutions and chelating agents alone did not dislodge alpha-glucosidase from membranes, but in combination with detergents were most effective in solubilizing active enzyme (0.1% sodium cholate (pH 8.0)/0.4 M sodium chloride). Purified alpha-glucosidase very rapidly hydrolized p-nitrophenyl alpha-D-glucopyranoside and sucrose. Maltose, maltotriose, isomaltose and isomaltotriose were hydrolized at slower rates, whereas beta-glucosides and polymeric alpha-glucans were not attacked. Other properties of the purified enzyme were as follows: Temperature optimum for catalysis = 39 +/- 1 degrees C; pH optimum = 6.8; molecular weight = 27,000 +/- 1000. alpha-Glucosidase is proposed to function in the endogenous metabolism of alpha-glucans provided extracellularly as carbon sources for growth of B. amyloliquefaciens.

Es wird eine 49jährige Frau vorgestellt, die nach einem Caudaneurinom einen sensiblen und motorischen Ausfall der Segmente S2–5 mit kompletter Harn- und Stuhlinkontinenz aufweist. Im April 1974 wurde eine Beckenboden-Implantatelektrode nach Caldwell operativ eingepflanzt, das Ergebnis nach 2jähriger Beobachtung entspricht einer Dauerheilung. Wirkungsmechanismus, Indikation und Erfolgsrate der Implantatelektrode werden besprochen.

A procedure is described for the isolation from the phototrophic procaryole Anacystis nidulans of [U-14C]-labelled glycogen, with high specific radioactivity,formed when NaH14CO3 was added to non-dividing cells that continued to photoassimilate CO2. [U-14C]-Labelled glycogen was then treated with isoamylase (EC 3.2.1.68), isoamylase plus beta-amylase (EC 3.2.1.2), or glucoamylase (EC 3.2.1.3) to give [U-14C]-labelled maltosaccharides, maltose-U-14C, or d-glucose-U-14C, respectively.

The fine structure of the glycogen from the blue-green alga Anacystis nidulans has been examined. After selective hydrolysis of all (1 yields 6)-alpha-D linkages by a bacterial isoamylase, the resulting mixture of linear chains was subjected to gel permeation chromatography. For purposes of comparison, the glycogens from Escherichia coli and Arthrobacter sp., amylopectin, phytoglycogen from sweet corn, and shell-fish glycogen were treated similarly. The profiles of the unit chains of A nidulans glycogen and phytoglycogen were closely similar. There was no close resemblance in the size distribution of unit chains for A. nidulans glycogen, other bacterial glycogens, and amylopectin.

Obwohl die Vorteile hypotensiver Anaesthesietechniken gerade in der Neurochirurgie auf der Hand liegen, schrecken auch heute noch viele selbst bei zwingender Indikation vor ihrer Anwendung zurück. Dies mag einerseits an der leider noch immer krassen Überbewertung des arteriellen Blutdrucks als Maß der Kreislauffunktion liegen, andererseits haften den „klassischen“ Verfahren der „künstlichen Blutdrucksenkung“ sicherlich einige Nachteile an, welche dazu führten, daß die „kontrollierte Hypotension“ zwar in den Händen einiger Erfahrener hervorragende Resultate lieferten, das Verfahren jedoch nie zu einer Routinetechnik ausreifen ließ.

Conference Article| February 01 1975 The Metabolic Role of Debranching Enzymes T. NORMAN PALMER; T. NORMAN PALMER 1Department of Biochemistry, Charing Cross Hospital Medical School, Fulham Palace Road, London W6 9RF, U.K.2Fachbereich Chemie der Philipps Universität Marburg, D-3550 Marburg (Lahn), Lahnberge, West Germany Search for other works by this author on: This Site PubMed Google Scholar GÜNTER WÖBER GÜNTER WÖBER 1Department of Biochemistry, Charing Cross Hospital Medical School, Fulham Palace Road, London W6 9RF, U.K.3Fachbereich Chemie der Philipps Universität Marburg, D-3550 Marburg (Lahn), Lahnberge, West Germany Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1975) 3 (1): 53–56. https://doi.org/10.1042/bst0030053 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn MailTo Cite Icon Cite Get Permissions Citation T. NORMAN PALMER, GÜNTER WÖBER; The Metabolic Role of Debranching Enzymes. Biochem Soc Trans 1 February 1975; 3 (1): 53–56. doi: https://doi.org/10.1042/bst0030053 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search This content is only available as a PDF. © 1975 Biochemical Society1975 Article PDF first page preview Close Modal You do not currently have access to this content.