BACKGROUND AND AIM:Continuous Subcutaneous Insulin Infusion (CSII) and Multiple Daily insulin Injections (MDI) are both strategies aiming to achieve a tight glycemic and metabolic control. However, the choice between them remains controversial. The aim of the present study was to compare the efficacy of MDI (three or more injections daily) with CSII on glycemic control in patients with Type 1 Diabetes Mellitus and assess satisfaction from treatment in the CSII group.MATERIAL AND METHODS:Seventeen patients with Type 1 Diabetes Mellitus on CSII (previously on MDI) and 17 patients on MDI, matched for age, gender, BMI and duration of diabetes, were retrospectively studied. Glucosylated Hemoglobin A1c (HbA1c), frequency of hypoglycaemias (assessed as self reported episodes), BMI and total units of insulin per day were evaluated at baseline and after 6 months in both groups. CSII group completed a questionnaire concerning motive for treatment selection, advantages, deficiencies and inconvenience at the end of the study. Satisfaction from treatment was assessed with a scale from 0 to10.RESULTS:CSII group had more hypoglycaemic episodes at baseline than MDI group (16.2+/-2.8 vs 2.8+/-1.3, p<0,001). HbA1c (8.4+/-0.5 before vs 7.3+/-0.4 after, p<0.05) and total hypoglycaemic episodes per month (16.2+/-2.8 before vs 8.7+/-2.3 after, p<0.05) significantly decreased in CSII group 6 months after baseline. On the contrary, total hypoglycaemic episodes per month were increased in MDI group (2.8+/-1.3 before vs 10.8 +/-2,6 after, p<0.05) in order to maintain HbA1c levels. No significant differences were observed in BMI in both groups. Total insulin demands were reduced in the CSII group (49.4+/-3.3 before vs 39.0+/-4.6 after, p<0.05) and remained unchanged in MDI group. None of the patients discontinued CSII therapy, while overall satisfaction rate in this group was high. The main motive for CSII selection was frequent hypoglycaemic episodes and glucose fluctuations (10/17). The majority of patients expressed their wish for incorporating glucose trend indicator and/or continuous glucose measurement into pump and reducing pump size (15/17). Most commonly stated advantage was improved flexibility, followed by greater freedom and decreased sense of physical restrictions (10/17). Inconvenience mainly derived from alarm malfunction and catheter or needle occlusion and was reported from a minority of patients (4/17).CONCLUSION:CSII group reported more hypoglycaemias than MDI group at baseline but 6 months later had significantly less hypoglycaemic events, while on the contrary, MDI group 6 months after baseline had more frequent and more severe hypoglycaemias. Although baseline hypoglycaemias are not equal between the two groups, we can assume that CSII group achieved less hypoglycaemic events along with significant reduction in HbA1c while utilising less insulin units.

BACKGROUND AND AIMIt has been reported that racial and ethnic (genetic make up), as well as socioeconomic differences may affect the results of kidney transplantation. Socioeconomic factors are quite difficult to differentiate from genetic factors. It is not surprising that a group with poorer access to health care, less private insurance and less income does less well with serious medical problems. The aim of this study was to compare the outcomes of kidney transplantations in Greek (G) and Albanian (A) patients.PATIENTS AND METHODSTwenty nine transplanted patients of Albanian ancestry were matched with 29 Greek patients retrospectively. Their mean age was 34 (G) and 31 (A) years, there were 21 men and 8 women in each group (G, A) and they received 26 kidneys from living related donors and 3 kidneys from cadaveric donors respectively. Arterial blood pressure (ABP), body weight (BW), serum creatinine, serum total protein and albumin, total cholesterol, HDL-cholesterol and triglycerides, 24 hour proteinuria were measured on 7th, 15th postoperative day, 1st , 3rd , 6th month and 1st year after transplant. BMI was calculated before and 1 year after transplantation and acute rejection episodes were recorded too. Methylprednizolone (MP), cyclosporine (CsA) dose /kg BW were calculated at baseline, 1, 3, 6, 12 months after transplant. Cumulative patient and graft survival at 1 and 5 years were calculated too.RESULTSPatient survival at 1 and 5 years was 100% / 93.1% and 100% /93.1% respectively (p: NS). Graft survival at 1 and 5 years was 100% / 93.10% and 93.75% / 86.45% respectively (p: NS). BW (but not BMI) and total cholesterol levels in Greek patients were higher compared to those of Albanian patients during the 1st post transplant year (p: 0.044 and p: 0.021 respectively). MP dose in A patients was higher during the first year (p: 0.05).CONCLUSIONPatients and graft survival do not present difference between G and A patients. There is significant difference on cholesterol profile between G and A patients. A larger number of transplants are possibly needed to allow us to draw firm conclusions.

P344 Aims: Donor age was identified recently as a major factor that determines long term outcome after kidney transplantation. However, the mechanism that is responsible for increased graft loss from the elderly kidney donors is unknown. The aim of our study was to evaluate the outcome of kidney grafts originated from elderly donors. Methods: In this study we compare 97 kidney grafts from living related donors (LRD) (group 1) with 34 cadaveric kidney grafts (group 2). In both groups donors were older than 60 years. Recipients transplanted from LRD were younger than those transplanted from cadaveric donors (group 1: recipients mean age 37,78 ± 9,30, group 2: recipients mean age 45,88 ± 11,21). Results: Patient survival rates at 1,3 and 5 years, respectively, were 95%, 95% and 93% (group 1) and 85%, 85%, 85% (group 2) (p=0.10, NS). Graft survival rates from LRD were 92%, 87% and 81% in group 1 versus 79%, 73% and 73% in group 2 (p=0.15, NS) and death censoring kidney graft survival was 96%, 91% and 86% in group 1 vs 93%, 86% and 86% in group 2 (p=0.10, NS). Acute rejection was a significant determinant for worse renal graft outcome (graft survival in recipients with at least one rejection episode (n1=35) at 1, 3 and 5 years : 94%, 78% and 67% vs graft survival in recipients without rejection episodes (n2=96) : 96%, 94% and 94% respectively (p= 0.0002). However, delayed graft function (DGF) itself, as well as donor – recipient HLA matching were not associated neither with the occurrence of early acute rejection episodes nor with worse renal graft outcome (graft survival in recipients with DGF (n3=22) at 1, 3 and 5 years: 85%, 85% and 85% vs graft survival in recipients without DGF (n4=109): 96%, 90% and 85% respectively, p=0.51, NS). Conclusions: We conclude that kidneys from older donors, irrespectively of their origin (LRD or cadaveric), can be successfully transplanted to younger patients. Acute rejection episodes in kidney grafts from elderly donors have a significant negative impact on long term renal graft outcome.

P380 Aims: Dyslipidemia contributes in the development of cardiovascular disease after kidney transplantation and chronic allograft dysfunction. The aim of this study was to investigate the effect of clinical and biochemical factors in the development of posttransplant dyslipidemia. Methods: Two hundred and ninety pts, 184 men and 106 women with mean age 38 year (range 16 – 71 years) were studied retrospectively. The effect of body weight (BW), serum creatinine (Scr), total protein (TP), serum albumin (SA), serum globulin (SG), age and sex on total cholesterol (TC), triglycerides (TR), and HDL-cholesterol (HDL-C) was studied. These parameters were recorded on the 1st, 3rd, 6th, 12th month, 1st, 2nd, 3rd, 4th and 5th year after transplantation. The effect of time on dialysis, the kind of dialysis, age, sex, total cholesterol (TCb), HDL-c (HDL-Cb) and triglycerides before (TRb) transplantation on the above mentioned lipids were studied too. The role of diabetes, hepatitis B and C was investigated. Repeated measures analysis, multiple regression analysis, independent t test, paired t test and descriptive statistics were used. Results: The TC, HDL-C and TR levels were 261.24±55.71 mg/dl / 58.61±19.41 mg/dl / 152.63±68.44 mg/dl, 262.18±55.30 mg/dl / 53.13±14.09 mg/dl / 170.73± 79.94 mg/dl, 263.53.02 mg/dl / 53.00±14.96 mg/dl / 158.85±86.07 mg/dl, 253.43± 49.78 mg/dl / 52.18±15.04 mg/dl / 152.53±76.62 mg/dl, 249±50.12 mg/dl / 52.84± 15.73 mg/dl / 147.22±71.04 mg/dl, 247.98±48.46 mg/dl / 52.60±15.33 mg/dl / 147.79±66.49 mg/dl, 243.13±41.30 mg/dl / 54.05±15.21 mg/dl / 141.74±57.51 mg/dl, 243.58±45.93 mg/dl / 53.30±14.42 mg/dl / 140.14±79.99 mg/dl on the above mentioned time respectively. During the 1st year follow up the age presented significant influence on TC (p=0.004) and TR level (p=0.001), the level of TCb Rt on TC (p=0.005) and the level of HDL-Cb Rt on HDL-C (p=0.021 from 3rd to 12th month). During the 5 year follow up there was significant influence of TP, SG and BW on TC (p=0.047, p=0.008, p=0.008 respectively). Patients with diabetes presented significantly higher levels of TC from non-diabetics during three year follow up (p:0.026). Conclusions: Age, TCb Rt, TP, SG and BW have significant influence on TC after renal transplantation. Age influences TR level and HDL-Cb affects sigificantly HDL-C during the 1st posttransplant year. Diabetes causes sigificantly higher levels of TC.