Acta Medica ScandinavicaVolume 181, Issue 2 p. 247-256 Human Lymphocyte Migration as a Parameter of Hypersensitivity MOGENS SøBORG, MOGENS SøBORG Medical Department A (Head: K. Brøchner-mortensen), and Medical Department A (Head: J. Hess Thaysen), Rigshospitalet, Copenhagen, DenmarkSearch for more papers by this authorGUNNAR BENDIXEN, GUNNAR BENDIXEN Medical Department A (Head: K. Brøchner-mortensen), and Medical Department A (Head: J. Hess Thaysen), Rigshospitalet, Copenhagen, DenmarkSearch for more papers by this author MOGENS SøBORG, MOGENS SøBORG Medical Department A (Head: K. Brøchner-mortensen), and Medical Department A (Head: J. Hess Thaysen), Rigshospitalet, Copenhagen, DenmarkSearch for more papers by this authorGUNNAR BENDIXEN, GUNNAR BENDIXEN Medical Department A (Head: K. Brøchner-mortensen), and Medical Department A (Head: J. Hess Thaysen), Rigshospitalet, Copenhagen, DenmarkSearch for more papers by this author First published: January/December 1967 https://doi.org/10.1111/j.0954-6820.1967.tb07255.xCitations: 404AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Citing Literature Volume181, Issue2January/December 1967Pages 247-256 RelatedInformation

Quantitative and qualitative assessment of the clinical disease manifestations in 41 primary Sjögren's syndrome (pSS) patients was performed according to a new classification model. Frequencies of subgrouped disease manifestations were as follows: 1) surface exocrine disease: 100%, 2) internal organ exocrine disease: 63%, 3) monoclonal B lymphocyte disease: 5%, 4) inflammatory vascular disease: 71%, 5) non-inflammatory vascular disease: 59%, 6) mediator induced disease: 98%. Summary scores for severity of surface exocrine disease correlated to the summary scores of all other disease manifestations (p = 0.02), to the summary scores of internal organ exocrine disease (p = 0.003), and to the summary scores of mediator induced disease (p = 0.03). Blood leucocyte counts showed significant negative correlations to levels of plasma IgG, serum IgA-RF, IgM-RF, anti-SSA/SSB antibodies, IL-6, and IL-1Ra. We conclude that the model made detailed analysis of the clinical presentation of pSS possible, and thus may assist in elucidating important pathobiological aspect of the disease.

Quantitative and qualitative assessment of the clinical disease manifestations in 41 primary Sjogren's syndrome (pSS) patients was performed according to a new classification model. Frequencies of subgrouped disease manifestations were as follows: 1) surface exocrine disease: 100%, 2) internal organ exocrine disease: 63%, 3) monoclonal B lymphocyte disease: 5%, 4) inflammatory vascular disease: 71%, 5) non-inflammatory vascular disease: 59%, 6) mediator induced disease: 98%. Summary scores for severity of surface exocrine disease correlated to the summary scores of all other disease manifestations (p=0.02), to the summary scores of internal organ exocrine disease (p=0.003), and to the summary scores of mediator induced disease (p=0.03). Blood leucocyte counts showed significant negative correlations to levels of plasma IgG, serum IgA-RF, IgM-RF, anti-SSA/SSB antibodies, IL-6, and IL-1Ra. We conclude that the model made detailed analysis of the clinical presentation of pSS possible, and thus may assist in elucidating important pathobiological aspect of the disease.

Objectives. The clinical features of 80 patients with primary Sjogren's syndrome (PSS) were revised in order to evaluate the descriptive and analytical facilities of a newly proposed model for classification of the exocrine and nonexocrine disease manifestations in PSS.Design. Retrospective, long-term (median 7.5 years follow-up) observational, clinical study.Setting. Patients were recruited from our Department, which is a tertiary referral centre for PSS patients.Subjects, Eighty patients fulfilling the Copenhagen criteria for keratoconjunctivitis sicca and/or xerostomia and followed between 1972 and 1991 were studied.Results. All patients had 'surface exocrine disease' and in 31% this was the only disease manifestation, 'Internal organ exocrine disease' was found in 25% of the patients, whilst 2.5% developed 'monoclonal B lymphocyte disease' (non-Hodgkin's lymphoma), 28% displayed 'inflammatory vascular disease', 25% noninflammatory vascular disease', 41% 'mediator-induced disease' and 2.5% 'autoimmune endocrine disease' (thyroiditis). In patients with 'internal organ exocrine disease' the frequencies of 'mediator-induced disease' (70%; P < 0.01) and 'inflammatory vascular disease' (50%; P < 0.03) were significantly higher than expected by chance. The level of immunoinflammatory activity (assessed by plasma IgG, serum ANA and focus scoring of minor labial salivary gland biopsies) correlated with the extent of clinical disease as assessed by the model.Conclusions. We conclude that this theoretically based model for classification of disease manifestations in PSS contains descriptive and analytic powers which may assist the clinical handling of these patients.

The ability of human lymphocytes to produce soluble mediator(s) with thrombocyte aggregating activity (TAA) was investigated in an in vitro technique. Isolated human mononuclear cells were stimulated with phytohaemagglutinin or purified protein derivative of tuberculin. The supernatants were investigated for lymphokine activity in a leucocyte migration inhibitory factor assay. Supernatants were then tested for their ability to aggregate human thrombocytes, and in contrast to the results of previous studies, we were not able to demonstrate any TAA. Most likely, lymphokines with TAA are not involved in the thrombotic processes seen in human cell‐mediated immune inflammatory reactions.

The activity of blood mononuclear cells (BMC) and synovial fluid mononuclear cells (SMC) from patients with rheumatoid arthritis (RA) and traumatic synovitis (TS) was assessed by means of [14C]thymidine incorporation and production of leukocyte migration inhibitory factor (LIF). When compared with normal controls, spontaneous LIF production by BMC was found in 5 of 9 TS patients, whereas spontaneous LIF production by rheumatoid arthritis BMC and by SMC from both patient groups was infrequently seen. ConA-induced LIF production by BMC and SMC from both patient groups did not differ significantly from that of normal controls. Thymidine incorporation by unstimulated SMC and BMC was low in both patient groups. After stimulation with polyclonal activators, SMC showed significantly reduced proliferation in comparison with BMC, but the responses to microbial antigens were equal to or higher than those of BMC. The proliferative responses of stimulated SMC from TS patients were higher than the responses displayed by stimulated SMC from RA patients.

SS-B antigen, purified from rabbit thymus, was used in an indirect enzyme immunoassay to demonstrate the presence of IgG-, IgA-, and IgM-type SS-B antibodies in sera from patients with well-defined and characterized chronic inflammatory connective tissue disease. High levels of antibodies to SS-B were found in patients with primary and secondary Sjögren's syndrome. Patients with Sjögren's syndrome secondary to systemic lupus erythematosus had significantly higher SS-B antibody values than patients with Sjögren's syndrome secondary to rheumatoid arthritis or patients with rheumatoid arthritis or systemic lupus erythematosus, alone. Two patients with rheumatoid arthritis without secondary Sjögren's syndrome also had a markedly elevated level of antibodies to SS-B. Antibodies of all immunoglobulin classes were found, although the highest values were either IgG- or IgM-type. In primary Sjögren's syndrome, antibody values to SS-B were higher in patients with HLA-Dw2 and/or HLA Dw3 than in those with other HLA-Dw types. We conclude that these antigens or specific immune-response genes close to the D region may be important for the development of antibodies to SS-B.

AllergyVolume 34, Issue 3 p. 131-133 Free Access Micro-Fungal Allergy Eva Weeke, Eva WeekeSearch for more papers by this authorGunnar Bendixen, Gunnar BendixenSearch for more papers by this author Eva Weeke, Eva WeekeSearch for more papers by this authorGunnar Bendixen, Gunnar BendixenSearch for more papers by this author First published: June 1979 https://doi.org/10.1111/j.1398-9995.1979.tb01561.xCitations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Citing Literature Volume34, Issue3June 1979Pages 131-133 RelatedInformation

Human lymphokines can elicit several effects associated with inflammation, e.g. leucocyte migration inhibition and fibrinolysis. These effects can be assessed in vitro by the leucocyte migration agarose technique (LMAT) and the leucocyte migration fibrinolysis technique (LMFT). The present study shows that preincubation of normal leucocytes with aprotinin, tranexamic acid and phenyl-methyl-sulfonylfluoride (PMSF) reduces or abolishes their migration inhibition response to leucocyte migration inhibition factor. The compounds exert this effect at non-toxic concentrations, which do not otherwise interfere with migration or fibrinolysis, and are non-toxic as estimated by PHA stimulation of lymphocytes. The LMFT is more sensitive to the modifying effect than the LMAT. The effect of aprotinin and tranexamic acid is reversible, the effect of PMSF is irreversible.

AllergyVolume 33, Issue 6 p. 289-291 Free Access In Vitro Diagnosis of Reagin-Mediated Allergy Bent Weeke, Bent WeekeSearch for more papers by this authorGunnar Bendixen, Gunnar BendixenSearch for more papers by this author Bent Weeke, Bent WeekeSearch for more papers by this authorGunnar Bendixen, Gunnar BendixenSearch for more papers by this author First published: December 1978 https://doi.org/10.1111/j.1398-9995.1978.tb01553.xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Volume33, Issue6December 1978Pages 289-291 RelatedInformation

AllergyVolume 33, Issue 3 p. 103-104 Free Access Pathogenic Importance of Lymphocyte Products Gunnar Bendixen, Gunnar BendixenSearch for more papers by this author Gunnar Bendixen, Gunnar BendixenSearch for more papers by this author First published: June 1978 https://doi.org/10.1111/j.1398-9995.1978.tb01518.xCitations: 1AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume33, Issue3June 1978Pages 103-104 RelatedInformation

The cellular immunity to Yersinia enterocolitica serotype 3 and crude human thyroid extract in 64 patients with thyroid diseases and 25 controls was studied by the leucocyte migration test. In the patient group as a whole and in patients with Graves' disease and nontoxic diffuse goitre a significantly reduced leucocyte migration towards Yersinia was found when compared with the controls. In controls the migration index was not related to the presence or titre of circulating yersinia antibodies, whereas the migration index of patients with yersinia antibodies was lower than the migration index of patients without yersinia antibodies as well as that of the controls. The leucocyte migration inhibition in two patients with recent yersinosis was normal during the recovery phase. In the presence of thyroid extract leucocyte migration inhibition differed only significantly in Graves' disease. However, a significantly positive correlation between inhibition of migration by thyroid extract and by Yersinia was found, while no correlation could be demonstrated in the controls. The cell-mediated immunity towards Yersinia in thyroid diseases thus demonstrated adds further evidence to the association between Yersinia and thyroid disease.