The lives and livelihoods of people around the world are increasingly threatened by climate-related risks as climate change increases the frequency and severity of high-impact weather. In turn, the risk of multiple hazards occurring simultaneously grows and compound impacts become more likely. The World Meteorological Organization (WMO) proposed the use of multi-hazard impact-based forecasting (IbF) to better anticipate and reduce the impacts of concurrent hazards, but as yet, there are few operational examples in the humanitarian sector. Drought is particularly susceptible to multi-hazard influences. However, challenges encountered in the development of drought IbF systems – including poor understanding of compound impacts and specific hazard-focused mandates – raise important questions for the feasibility of multi-hazard IbF as envisioned by the WMO. With these challenges in mind, we propose an interim approach in which real-time assessment of dynamic vulnerability provides a context for drought-based IbF. The incorporation of dynamic vulnerability indicators account for the local effects of non-drought hazards, whilst the use of a drought-based system facilitates effective intervention. The proposed approach will improve our understanding of compound events, enhance adoption of IbF in the humanitarian sector, and better mitigate the impacts of concurrent hazards.

Drought and food security crises heighten risks to lives and livelihoods in East Africa. In recent years, a shift towards acting in advance of such events has gained momentum, notably among the humanitarian and development community. This shift is premised on tools that link climate forecasts with pre-agreed actions and funding, known as Forecast-based Action (FbA), or anticipatory action more widely. While FbA approaches have been developed by a number of humanitarian agencies, the key to scaling-up is mainstreaming these approaches into national risk management systems. This paper addresses this gap in the context of drought risk management in Kenya. We analyse Kenya's current drought management system to assess the potential usability of climate forecast information within the existing system, and outline steps towards improved usability of climate information. Further, we note the critical importance of enabling institutions and reliable financing to ensure that information can be consistently used to trigger early action. We discuss the implications of this for scaling-up FbA into national risk management systems.

Background: In Sub-Saharan Africa, the supply of affordable, accessible and safe drinking water particularly in rural areas lags behind global progress. Achieving the universal and equitable access objectives warrants an understanding of household behavior in relation to water services. Purpose of the study: The aim of the study was to investigate the factors that influence willingness to pay for improved water service provision in a rural context in Kenya. Methods: A total of 601 households in Baringo and Samburu Counties were randomly selected using probability proportional to size technique. The data was analyzed using descriptive statistics and logit regression. Results: The empirical result shows that demographic characteristics of household, water source, quality and quantity, children responsible for fetching water, water access challenges and frequency of diarrhea are significant variables that explain willingness to pay. Conclusion: The findings conclude with important policy recommendations; (i) demand-driven approaches in the design of water supply projects, (ii) enhanced water quality management, and (iii) awareness creation to achieve social and health benefits which can facilitate long-term access and sustainability.

Kenya is mostly affected by floods during the March-April-May (MAM) and October-November-December (OND) rainfall. This often occurs along river basins such as the Tana river basin, leading to disruption of people's livelihoods, loss of lives, infrastructure destruction and interruption of economic activities. This study used openly available data on flood exposure, vulnerability, lack of coping capacity, flood impacts and observed satellite rainfall to analyse and predict forecast-based impacts in Tana river. Earth observation satellites including LANDSAT, sentinel 1 and 2 were acquired based on credible flood event dates to validate flood exposure and flood events. The community risk assessment (CRA) approach was used to delineate communities at high risk of floods using combination of data on vulnerability, flood exposure and lack of coping capacity. Using an ordinary least squares (OLS) predictive model, observed satellite rainfall was used as a covariate in order to predict flood impacts on communities with high flood risk scores in Tana river. Weighted scores from the CRA dimensions were summed up with forecasted hazards from the OLS model in order to derive a flood impact-based forecast. The flood impact information is to be used in forecast-based action through early warning, early action protocols thereby reducing impacts of potential floods in communities living in high flood risk areas based on the flood risk map.

Tree damage was assessed in 1540 Acacia species (A. xanthophloea) in Olpejeta Conservancy. Using belt transects measuring (20 x 50 m), trees in the sample sites (closed and open) were counted. Measurements were made of Acacia tree count, recruits count, diameter at breast height, and mortality for both adult trees and seedlings. Densities were significantly higher in enclosed areas (398.3 ± 16.60 trees/ha) than open areas (243.3 ± 10.13) trees/ha. Elephants recorded the highest damage to the sampled trees with 54.55% damage while human beings caused 12.85% of the damage. Browsing was the main form of damage in open areas. Mean height for enclosed and open sites was 3.77 ± 4.61 and 5.35 ± 8.81, respectively. These findings suggest that herbivory damage did not have a significant effect on seedling regeneration but rather had an effect on population structure. Debarking caused by elephants occurred in the open areas with the highest being 96% of the sampled trees in the 11-25% damage class. The diameter class quotients fluctuated, an indication of the unstable population thus suggesting the impact of herbivory on population structure. Key recommendations include the creation of more enclosure zones, winning back more space by controlling the densities of browsers, long-term monitoring and the promotion of recruitment and regeneration rather than reducing mature tree loss.

Background and Objective: The leaf of Homalomena sagittifolia was reported to have anti-inflammatory, antimicrobials, narcotic, violent intoxication and hallucinogen effects.This study highlights isolation, identification and biological activities of two compounds from the leaves of H. sagittifolia.Methodology: Two isolates were investigated for their inhibitory effects against cyclooxygenase and acetylcholinesterase enzymes.They were also tested for antimicrobial effects against five pathogenic bacterial strains using the micro-dilution assay.The structure of the two isolates were elucidated by interpretation of spectroscopic data and previous available reports in literature.They were identified to be trans-phytol (1) and diacylglyceroglycolipid (2).The compounds were investigated for their anti-inflammatory, anticholinergic and antimicrobial effects using the cyclooxygenase, the microplate and the antimicrobial micro-dilution assays, respectively.Results: Compound 2 possessed good activity against both COX-1 (IC 50 = 38) and COX-2 (IC 50 = 48).The IC 50 values observed with the indomethacin were 4.1 and 181 µM against COX-1 and COX-2, respectively.The two compounds also inhibited activity of acetylcholinesterase with an IC 50 values of 8.6 µM (2), 24 µM (1) and 3.3 µM (galanthamine).Compound 2 showed remarkable activity against Gram-negative bacteria Pseudomonas stutzeri and Klebsiella pneumoniae with an MIC value of 98 µM.The MIC values recorded for tetracycline were 87 and 175 µM against P. stutzeri and K. pneumonia, respectively.Conclusion: These results indicated the potential pharmacological properties of the leaves of H. sagittifolia and supported the traditional uses of the plant.Further studies are needed to understand its molecular interactions.This may lead to the development of standardized crude drugs and/or nutraceutical agents.

BACKGROUND:Pleurostylia capensis is a large tree that can reach the maximum height of 20 m long, and it have been traditionally used as cosmetic, for steam bath, ritual body wash, and as a purgative to treat symptoms of witchcraft. Using ethanol, chloroform, dichloromethane (DCM), ethyl acetate (EA), and water extracts, leaves, bark and roots of Pleurostylia capensis were investigated scientifically for their effectiveness in antimicrobial, antioxidant and anti-inflammatory activities using standard methods.MATERIALS AND METHODS:The extracts were evaluated for antimicrobial activity against Gram positive (Staphylococcus aureus, Bacillus cereus, and Mycobacterium smegmatis), Gram negative (Escherichia coli, Klebsiella pneumonia, Klebsiella oxytoca, Streptococcus pyogenes, Pseudomonas aeruginosa and Salmonella typhimurium), and Candida albicans. The antioxidant activity was investigated using 2, 2-diphenlyl-1-picrylhadrazyl (DPPH), free radical scavenging assay. The anti-inflammatory activity of P. capensis extracts was evaluated against both cyclooxygenase enzymes (COX 1 and 2).RESULTS:The ethyl acetate extracts of P. capensis showed a strong antimicrobial activity against B. cereus, K. pneumonia, S. pyogenes, and M. smegmatis with MIC value of 0.39 and 0.78 mg/ml. While the ethanol bark extract was most active against M. smegmatis with MIC value of 0.78 mg/ml; the least potent activity was observed with dichloromethane, chloroform and water extracts, with an MIC value ranging from 1.56 mg/ml to 50.0 mg/ml. The plant extracts proved to be good antioxidant agent, whereas extracts of ethanol were the most active, with IC50 ranging from 1.00 to 1.74 µg/ml, which is lower, and in close range to Vitamin C (1.40 µg/ml).CONCLUSIONS:Its moderation to potent inhibitory activity was observed in all extracts. Ethanol and dichloromethane extracts were among the most potent when compared to water and petroleum ether extracts. The water extracts showed to be nontoxic on the Hek cell line with an IC50 value of 204.0, and 207.3 µg/ml (roots and bark) respectively. The dichloromethane, ethyl acetate, chloroform and ethanol extracts showed to be toxic on the Hek cell, with IC50 range from 5.94 to 42.91µg/ml. The results obtained indicate the effectiveness of these plants.

Plants have played significant roles as medicine during pregnancy, birth, and postpartum care in many rural areas of the world. In addition to this, plants have been used for centuries to treat infertility and related reproduction problems. The aim of this paper was to review the current status of plant species used in maternal healthcare, including infertility, in South Africa, in terms of scientific evaluation for efficacy and safety. In addition to this, the role of medicinal plants as a tool in achieving the MDG5 of reducing maternal mortality by 2015 was evaluated. A search was done with the aid of Google Scholar, PubMed, Science Direct, peer-reviewed papers, and books, using keywords such as child birth, labour pain, maternal health, maternal mortality, menstrual pains, and postpartum. The plants listed in the different research articles were classified according to their use and the target effect of a plant extract or compound on reproductive function. Eighty-four plant species were found to be used to treat infertility and related problems. Twenty plant species are used during pregnancy, while 26 plant species are used to ease childbirth. For postpartum healing and any problems after childbirth, nine plant species were recorded. Unhealthy pregnancy and birth complications are among the factors that contribute to the loss of cognitive potential in the developing world's children, condemning them to impoverished lives. The best way to keep a country poor is to rob its children of their full developmental potential. In this respect, medicinal plants play a significant role in reducing maternal mortality and ensuring the birth of healthy children.

Ethnopharmacological relevance: Traditional remedies from plants have been use to treat male reproductive related disorders for ages in South Africa.Aim: This study reviewed the current status of medicinal plants used in male reproductive healthcare as well as their effectiveness as a mode of treatment.Methodology: A detailed literature search was done by consulting books, peer-reviewed papers, scientific databases such as Scopus, Science direct and PubMed, and Google scholars. Keywords such as aphrodisiacs, conception, erectile dysfunction, fertility, infertility and sterility in relation to medicinal plants were used during the search. Plant species were selected based on their traditional use in different aspects of male reproductive healthcare.Results: A total of 61 plants species were found to be used in treating male impotency or as aphrodisiacs. However, only six species have been evaluated scientifically and these plants showed significant activities either in promoting conception by improving fertility and promoting erection or in contraception by decreasing sperm motility, virility and membrane integrity.Conclusion: The importance of herbal products in the treatment of male infertility and related sexual disorders is undeniable. Scientific evaluations of the six species have proved the empirical use of these plants in the improvement of male sexual disorders as well as validating their traditional uses. (C) 2012 Elsevier Ireland Ltd. All rights reserved.

Ethnopharmacological relevance: Four species of Podocarpus are used in traditional medicine both in human and animal healthcare in South Africa. In vitro pharmacological screening of leaf and stem extracts of these species exhibited potent antimicrobial, anti-inflammatory, anti-tyrosinase, anthelmintic, acetylcholinesterase inhibitory and antioxidant activities.Aim of the study: To investigate the mutagenicity, antimutagenicity and cytotoxicity effects of leaf and stem extract of South African Podocarpus species.Material and methods: The mutagenicity and cytotoxic effects of extracts from four species of Podocarpus were tested using the Salmonella/microsome assay with and without metabolic activation, based on the plate-incorporation method and neutral red uptake (NRU) assay respectively. Five Salmonella typhimurium tester strains; TA98, TA100, TA102, TA1535 and TA1537 were used for mutagenicity testing. The relative cytotoxicity of the extracts was assessed by determining their NI50 values (50% inhibition of NRU).Results: The extracts did not show any mutagenic effects against all the tester strains with or without metabolic activation. All extracts demonstrated a strong antimutagenic effect on the mutations induced by 4NQO, decreasing its mutagenic effect in a dose-dependent manner. Strong cytotoxic effects were exhibited by petroleum ether extracts as compared to 80% ethanol extracts. When HepG2 cells were in contact with plant extracts in an increasing concentration, slopes of NRU decreased (highest-lowest %) following a concentration-dependent pattern. For 80% ethanol extracts, the most toxic extract in terms of percentage viability was leaves of Podocarpus falcatus whereby at 0.2 mg/ml, the viability of the cells was 38.9%. Stem extract of Podocarpus latifolius was the most toxic among PE extracts, giving a percentage viability of 46.4 at 0.1 mg/ml.Conclusion: Absence of mutagenicity does not indicate lack of toxicity, as was observed from these extracts. These findings will help in assessing the safety measures to be considered in the use of these species and also the need to determine the cytotoxic potential of these species against various forms of human cancer cells. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

A new sesquiterpenoid, 1 alpha,4 beta,7 beta -eudesmanetriol (1), was isolated together with the known compounds 1 beta,4 beta,7 beta -eudesmanetriol (2) and oplopanone (3) from the rhizomes of Homalomena sagittifolia. The structures of these compounds were determined by extensive spectral analyses. The compounds 1 and 2 inhibited growth of Pseudomonas stutzeri with a MIC value of 117 mu M when evaluated for antibacterial activity using the minimum concentration assay. Both these compounds showed remarkable activities against acetylcholinesterase enzyme with IC50 values ranging between 25 and 26 mu M. The isolation of these sesquiterpenoids and their biological activities observed in this study support the reported traditional uses of H. sagittifolia for the treatment of microbial related diseases and central nervous system disorders.

Ethnopharmacological relevance: Species of Podocarpus are used traditionally in their native areas for the treatment of fevers, asthma, coughs, cholera, chest complaints, arthritis, rheumatism, venereal diseases and distemper in dogs.Aims of the study: To investigate the antioxidant, anti-inflammatory and anti-tyrosinase activities of four Podocarpus species, Podocarpus elongatus, Podocarpus falcatus, Podocarpus henkelii and Podocarpus latifolius, used in traditional medicine in South Africa. Phytochemical analysis to determine the phenolic contents was also carried out.Materials and methods: DPPH, FRAP and beta-carotene-linoleic acid assays were used to determine the antioxidant/radical scavenging activities of these species. Anti-inflammatory activity of these species was assayed against two cyclooxygenase enzymes (COX-1 and COX-2). Tyrosinase inhibition activity was analysed using the modified dopachrome method with L-DOPA as the substrate. Phenolics were quantitatively determined using spectrophotometric methods.Results: Stems of Podocarpus latifolius exhibited the lowest EC50 (0.84 mu g/ml) inhibition against DPPH. The percentage antioxidant activity based on the bleaching rate of beta-carotene ranged from 96% to 99%. High ferric reducing power was observed in all the extracts. For COX-1, the lowest EC50 value was exhibited by stem extracts of Podocatpus elongatus (5.02 mu g/ml) and leaf extract of Podocarpus latifolius showed the lowest EC50 against COX-2 (5.13 mu g/ml). All extracts inhibited tyrosinase activity in a dose-dependent manner with stem extract of Podocarpus elongatus being the most potent with an EC50 value of 0.14 mg/ml. The total phenolic content ranged from 2.38 to 6.94 mg of GAE/g dry sample.Conclusion: The significant pharmacological activities observed support the use of these species in traditional medicine and may also be candidates in the search for modern pharmaceuticals in medicine, food and cosmetic industries. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

The genus Podocarpus sensu latissimo (s.l.) was initially subdivided into eight sections. However, based on new information from different morphological and anatomical studies, these sections were recognised as new genera. This change in nomenclature sometimes is problematic when consulting ethnobotanical data especially when selecting plants for pharmacological screening, thus there is a need to clear any ambiguity with the nomenclature. Species of Podocarpus s.l. are important timber trees in their native areas. They have been used by many communities in traditional medicine and as a source of income. Podocarpus s.l. is used in the treatment of fevers, asthma, coughs, cholera, distemper, chest complaints and venereal diseases. Other uses include timber, food, wax, tannin and as ornamental trees. Although extensive research has been carried out on species of Podocarpus s.l over the last decade, relatively little is known about the African species compared to those of New Zealand, Australia, China and Japan. Phytochemical studies have led to the isolation and elucidation of various terpenoids and nor- and bis-norditerpenoid dilactones. Biflavonoids of the amentoflavone and hinokiflavone types have also been isolated. Nor- and bis-norditerpenes are said to be taxonomic markers for this genus. Recent in vitro and in vivo studies have shown antitumor, antimicrobial, anti-inflammatory, antioxidant, larvicidal, plant and insect growth regulation activities. Various studies have yielded important natural bioactive products and two of them are worth mentioning. Taxol, a significant anticancer agent has been isolated from Podocarpus gracilior and totarol, a diterpenoid isolated from various species and now commercially produced as a potent antibacterial and antioxidant agent. Findings from this review supports the use of an ethnobotanical and chemotaxonomical approach in selecting plants for pharmacological screening since most of the species in the different morphological groups have similar uses. Also the isolated compounds have chemotaxonomic value amongst the groups. Some of the biological activities identified from extracts and compounds isolated from Podocarpus s.l. support the rationale behind the medicinal uses of these species.

In South Africa, heavy metal contamination of agricultural soils is on the increase primarily due to excessive application of fertilizers, sewage disposal and mining activities. The aim of this study was to investigate the effect of cadmium (Cd) on plant growth and biological activity. The medicinal plant Merwilla plumbea [Syn. Merwilla natalensis (Syn. Scilla natalensis)] was selected due to the high demand for its bulbs in the traditional medicine markets. Low levels of Cd (2 mg/L) significantly reduced fresh mass of leaves, bulbs and roots in comparison to the control. Although most of the Cd was stored in the roots, the bulbs, which are used medicinally, accumulated 7.1, 5.9 and 11.6 mg/kg when grown in sand watered weekly with 2, 5 and 10 mg Cd/L respectively. The bulbs of M. plumbea contained 24-fold more Cd than the World Health Organization guideline of 0.3 mg Cd/kg, when irrigated with 2 mg Cd/L. The bulb extracts showed increased antibacterial activity against the Grain-positive bacterium Bacillus subtilis at 2 mg Cd/L, The plants treated with 10 mg Cd/L showed an increased antibacterial activity against B. subtilis, Klebsiella pneumoniae and Staphylococcus aureus in comparison to non-Cd-treated plants (control). However, there was no change in antibacterial activity of the various extracts against the Grain-negative bacterium Escherichia coli. The ability of M. plumbea to accumulate Cd not only raises concern for consumer safety, but also the quality of medicinal plants sold may be in question. (C) 2009 SAAB. Published by Elsevier B.V. All rights reserved.

Ethnopharmacological relevance: Several species of Podocarpus (Podocarpaceae) are utilized in treating ailments across the world. In Africa, four species are used traditionally in both animal and human health.Aim of the study: To investigate the antimicrobial activity of Podocarpus species against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Candida albicans.Materials and methods: Six solvents of varying polarity were used for extraction. Antibacterial activity was assessed using the microdilution bioassay and for antifungal activity, the microdilution bioassay and (M27-P) broth dilution were used.Results: All species exhibited antimicrobial activity with MIC values of less than 1 mg/ml. Inhibition against Gram-positive bacteria was stronger with an MIC value of 98 mu g/ml while for Gram-negative bacteria, the highest inhibition was against Klebsiella pneumoniae with an MIC value of 0.33 mg/ml. All species exhibited strong antifungal activity with the best MIC being 30 mu g/ml after 48 h.Conclusions: All four species exhibited strong inhibition against all tested microbials, based on Aligiannis et al. [Aligiannis, N., Kalpotzakis, E., Mitaku, S., Chinou, I.B., 2001. Composition and antimicrobial activity of the essential oils of two Origanum species. journal of Agricultural and Food Chemistry 40, 4168-4170] classification they can be classified as strong inhibitors. (C) 2008 Elsevier Ireland Ltd. All rights reserved.