Journal of Veterinary Pharmacology and TherapeuticsVolume 18, Issue 3 p. 236-238 Uptake of N4d-glucopyranosylsulphamethazine by rat renal cortical slices Y. WANG, Y. WANG *Departments of Pharmacology, University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorR. GRIGG, R. GRIGG †Departments of Organic Chemistry, University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorH. SYMONDS, H. SYMONDS ‡Departments of Animal Physiology & Nutrition, University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorC.J. BOWMER, C.J. BOWMER *Departments of Pharmacology, University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this author Y. WANG, Y. WANG *Departments of Pharmacology, University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorR. GRIGG, R. GRIGG †Departments of Organic Chemistry, University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorH. SYMONDS, H. SYMONDS ‡Departments of Animal Physiology & Nutrition, University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this authorC.J. BOWMER, C.J. BOWMER *Departments of Pharmacology, University of Leeds, Leeds LS2 9JT, UKSearch for more papers by this author First published: June 1995 https://doi.org/10.1111/j.1365-2885.1995.tb00584.xAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Volume18, Issue3June 1995Pages 236-238 RelatedInformation

The role of the vertebral arteries in delaying loss of sensibility following neck sticking in slaughter calves was investigated. Vertebral artery blood flow was measured using probes before, during and after electrical stunning and slaughter. Systemic blood pressure, electrocorticogram, visually evoked responses and the occurrence of carotid occlusions were also recorded. When carotid occlusion occurred, the time to onset of brain failure was delayed based on the development of an isoelectric state. In addition, when carotid occlusion occurred the mean arterial blood pressure was sustained for longer following slaughter, and concurrently vertebral artery blood flow could be maintained at about 30% of its initial level for up to 3 min. In some animals vertebral artery flow increased substantially following sticking. When chest sticking was used no occlusion of vessels occurred, mean arterial blood pressure fell promptly (within 8 s) and the onset of an isoelectric state did not extend beyond one minute. In addition, visually evoked responses were not present after 5 s following chest sticking.

Absorption of the N4-D-glucose conjugate of sulphamethazine (glucose-SMZ, 0.5 mM) by isolated everted sacs of the rat small intestine was studied at 37-degrees and pH 6.6. Phlorizin (0.5-2.0 mM) significantly reduced (P < 0.05) both mucosal and serosal transfer of glucose-SMZ and inhibition of mucosal transfer appeared to be concentration-dependent. Phloretin (0.5 mM) and removal of Na+ from the incubation medium also diminished absorption of glucose-SMZ. Furthermore, D-glucose (0.5 and 5.0 mM) inhibited mucosal and serosal transfer of the glycoside. The results suggest the D-glucose/Na+ cotransporter mediates absorption of glucose-SMZ from the small intestine of the rat. Thus, glucose-SMZ might be bioavailable from ingested tissues in which it is present.