Strain DCY37 T was isolated from a soil sample of a ginseng field in the Republic of Korea and characterized in order to determine its taxonomic position. Cells were Gram-staining-positive, heterotrophic, strictly aerobic, non-motile short rods. 16S rRNA gene sequence analysis revealed that strain DCY37 T belongs to the genus Microbacterium . According to 16S rRNA gene sequence analysis, it is closely related to Microbacterium aerolatum DSM 14217 T (98.8 %), Microbacterium hydrocarbonoxydans DSM 16089 T (98.5 %), Microbacterium natoriense JCM 12611 T (98.5 %), Microbacterium foliorum (98.4 %) and Microbacterium phyllosphaerae (98.3 %). However, DNA–DNA hybridization studies showed reassociation values of less than 70 % between representative strains and DCY37 T . The DNA G+C content was 64.5 mol%. Strain DCY37 T possessed chemotaxonomic markers that were consistent with classification in the genus Microbacterium , i.e. MK-12 and MK-13 as the major menaquinones and anteiso-C 15 : 0 , anteiso-C 17 : 0 and iso-C 16 : 0 as the predominant cellular fatty acids. The major cell wall sugars were ribose, xylose and galactose. The diamino acid in cell-wall hydrolysates of strain DCY37 T was ornithine and major cell-wall amino acids were alanine, glycine, d -glutamic acid and serine. The major polar lipids were glycolipid, phosphatidylglycerol, diphosphatidylglycerol and unknown aminolipids. Based on these data, DCY37 T (=KCTC 19526 T =JCM 15516 T ) should be classified as the type strain of a novel species of the genus Microbacterium , for which the name Microbacterium ginsengiterrae sp. nov. is proposed.

Strain DCY45(T) was isolated from soil of a ginseng field in Pocheon Province, Korea. Strain DCY45(T) was Gram-negative, oxidase- and catalase-positive, motile and rod-shaped and produced yellow pigments on R2A agar. The organism grew optimally at 30 °C and at pH 7.0. The G+C content of the genomic DNA was 65.4 mol%. The predominant respiratory quinone was Q-8. The major fatty acids were iso-C(17 : 1)ω9c, iso-C(16 : 0) and iso-C(15 : 0). Phylogenetic analysis based on the 16S rRNA gene sequence was used to determine the taxonomic position of strain DCY45(T), which is most closely related to species of the genus Rhodanobacter, with similarity levels of 96.0-98.4 %; DNA-DNA relatedness with related strains was lower than 60 %. Strain DCY45(T) differed significantly from related type strains in phenotypic characteristics. On the basis of these phenotypic, genotypic and chemotaxonomic studies, strain DCY45(T) represents a novel species of the genus Rhodanobacter, for which the name Rhodanobacter soli sp. nov. is proposed. The type strain is DCY45(T) (=KCTC 22620(T) =JCM 16126(T)).

A bacterial strain (designated KMY03T) that possesses beta-glucosidase activity was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. The bacterium was found to comprise Gram-negative, rod-shaped, motile cells with unipolar polytrichous flagella. On the basis of 16S rRNA gene sequence similarity, strain KMY03T was shown to belong to the family Burkholderiaceae of the Betaproteobacteria, being most closely related to Burkholderia caledonica LMG 19076T (97.8%), Burkholderia terricola LMG 20594T (97.5%), Burkholderia xenovorans LMG 21463T (97.4%) and Burkholderia phytofirmans LMG 22146T (97.3%). Chemotaxonomic data (major ubiquinone, Q-8; major fatty acids, C17:0 cyclo, C16:0, C19:0 cyclo omega8c and summed feature 2) supported the affiliation of the novel strain with the genus Burkholderia. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed the strain to be differentiated genotypically and phenotypically from Burkholderia species with validly published names. On the basis of these data, strain KMY03T represents a novel species of the genus Burkholderia, for which the name Burkholderia ginsengisoli sp. nov. is proposed. The type strain is KMY03T (=KCTC 12389T=NBRC 100965T).