This study evaluates a mixed-culture microbial fuel cell (MFC) as a rapid, real-time biosensor for detecting water toxicity caused by heavy metals and benzene, toluene, ethylbenzene, and xylene (BTEX) compounds. A dual-chamber MFC (200 mL) equipped with carbon fiber electrodes was operated with an electroactive biofilm on the anode, and toxicity responses were quantified using inhibition ratios (IR) derived from voltage suppression following contaminant exposure. The biosensor effectively detected copper (Cu2+) and mercury (Hg2+) over a concentration range of 2-20 mg L-1, exhibiting strong linear relationships (R2 = 0.9928 and R2 = 0.9811, respectively). Mixed-culture biofilms showed a 1.64-fold increase in sensitivity compared to pure cultures and demonstrated stable baseline signals. The biosensor also responded linearly to benzene and xylene (R2 = 0.9876 and R2 = 0.9811) with rapid response times of 1.0-3.5 min. Microbial community analysis indicated increased richness and evenness following MFC operation, supporting biofilm stability during short-term exposure. The developed biosensor is intended for early-warning and process-health monitoring in industrial and wastewater systems, where rapid detection of toxicity spikes is critical. These findings demonstrate the feasibility of MFC-based biosensors as low-cost, in situ tools for integrative toxicity surveillance.

The phaseout of per- and polyfluoroalkyl substances (PFAS) in firefighting foams has motivated the adoption of fluorine-free foams (F3), yet their environmental fate remains poorly understood. This study provides the first comprehensive assessment of F3 biodegradation under both aerobic and anaerobic conditions, combining modified OECD protocols, high-resolution mass spectrometry (HRMS), and microbial community profiling to elucidate surfactant degradation pathways, byproduct formation, and ecological impacts. Aerobic systems achieved greater than 80% bulk dissolved organic carbon (DOC) removal for two commercial F3 formulations within 28 days, yet targeted analyses revealed persistent, hydrophobic surfactants (e.g., ethylene glycol dodecyl ether, EGDE) in sludge phases (2-5 μg L-1) and transient byproducts like short-chain glycol ethers. Anaerobic degradation diverged sharply with minimal DOC reduction (<5%), limiting surfactant transformation, including sulfate reduction-driven hydrogen sulfide generation and accumulation of alkylamine intermediates (e.g., N-methyldodecylamine). Non-target HRMS screening identified 21 byproducts. Nine were exclusive to late-stage samples at day 60, with unknown peaks constituting 15-20% of residual DOC as uncharacterized compounds absent from formal assessments. Microbial diversity was reduced by 79% in anaerobic systems, selecting for specialist taxa (e.g., Azospira, Nitrospira) with the potential for surfactant hydrolysis. In contrast, aerobic communities retained metabolic flexibility but showed concentration-dependent inhibition. These findings challenge the adequacy of standardized biodegradability tests, which overlook sludge-phase residuals, redox-specific byproducts, and non-target compounds. This work shows that assumed "readily biodegradable" F3 surfactants may also leave persistent residues, necessitating advanced frameworks with phase-specific analytics and pathway prediction tools to ensure replacements reduce and do not redistribute risks to water quality and ecosystems.

Ultraviolet-light emitting diodes (UV-LEDs) offer several advantages over conventional mercury-based UV lamps, including wavelength selectivity, compact size, design flexibility, instant on/off, power output adjustment, and mercury-free operation. These features position UV-LEDs as ideal candidates for point-of-use (POU) water disinfection systems, particularly in decentralized or resource-limited environments. In this study, we evaluated the microbial inactivation performance and energy efficiency of a bench-scale flow-through UV-LED POU system using indigenous heterotrophic plate count (HPC) bacteria, E. coli, and MS2 bacteriophage. The system was tested under various flow rates (1–4 L/min) and wavelength configurations (265 nm, 278 nm, and dual-wavelength combinations). MS2 bacteriophage was further used in collimated beam testing to validate UV-fluence-response curves and to estimate delivered doses in the flow-through POU device. HPC inactivation was enhanced under dual-wavelength conditions, suggesting wavelength-specific synergy, while E. coli showed high susceptibility across all wavelength configurations, achieving >2-log inactivation at significantly reduced UV-LED power (1/6 of that required for HPC) even at 4 L/min. Specific energy consumption analysis showed energy demands as low as 0.032–0.053 kWh/m3 for achieving 4-log inactivation of E. coli, with an estimated annual operating cost for UV-LED irradiation below $1.70. These findings demonstrate the potential of UV-LED-based POU devices as safe, energy-efficient, and cost-effective technologies for decentralized water treatment.

Hot water systems are the most frequent environment associated with the prevalence and growth of opportunistic premise plumbing pathogens (OPPPs). Previous studies identified water heaters as a source of waterborne diseases and concluded that design variables may contribute to their prevalence. A multifaceted approach was used to investigate the vertical stratification of the microbiome and selected OPPPs in an electric water heater tank connected to a simulated home plumbing system. Results show that the microbiome is highly diverse with evidence of temperature stratification and temporal structuring influenced by the partial drainage of the water tank. Representatives of the Mycobacterium spp. were the most prevalent taxa, followed by Legionella spp., and a relatively low prevalence of free-living amoeba Vermamoeba vermiformis. Higher concentrations of Legionella pneumophila at the bottom of the tank indicated the potential growth and protection of this opportunistic pathogen at this location. Overall, partial drainage of the water tank (60% of the tank capacity) did not significantly mitigate the microbiome and selected OPPPs. The outcome of this study sheds light on the role of vertical stratification on water quality, and demonstrates the resilience of the microbial community residing in an electric water heater tank and the implications for public health.

The occurrence of harmful algal blooms (HABs) in freshwater environments has been expanded worldwide with growing frequency and severity. HABs can pose a threat to public water supplies, raising concerns about safety of treated water. Many studies have provided valuable information about the impacts of HABs and management strategies on the early-stage treatment processes (e.g., pre-oxidation and coagulation/flocculation) in conventional drinking water treatment plants (DWTPs). However, the potential effect of HAB-impacted water in the granular media filtration has not been well studied. Biologically-active filters (BAFs), which are used in drinking water treatment and rely largely on bacterial community interactions, have not been examined during HABs in full-scale DWTPs. In this study, we assessed the bacterial community structure of BAFs, functional profiles, assembly processes, and bio-interactions in the community during both severe and mild HABs. Our findings indicate that bacterial diversity in BAFs significantly decreases during severe HABs due to the predominance of bloom-associated bacteria (e.g., Spingopyxis, Porphyrobacter, and Sphingomonas). The excitation-emission matrix combined with parallel factor analysis (EEM-PARAFAC) confirmed that filter influent affected by the severe HAB contained a higher portion of protein-like substances than filter influent samples during a mild bloom. In addition, BAF community functions showed increases in metabolisms associated with intracellular algal organic matter (AOM), such as lipids and amino acids, during severe HABs. Further ecological process and network analyses revealed that severe HAB, accompanied by the abundance of bloom-associated taxa and increased nutrient availability, led to not only strong stochastic processes in the assembly process, but also a bacterial community with lower complexity in BAFs. Overall, this study provides deeper insights into BAF bacterial community structure, function, and assembly in response to HABs.

The COVID-19 pandemic has resulted in significant changes in our daily lives, including the widespread use of face masks. Face masks have been reported to reduce the transmission of viral infections by droplets; however, improper use and/or treatment of these masks can cause them to be contaminated, thereby reducing their efficacy. Moreover, regular replacement of face masks is essential to maintaining their effectiveness, which can be challenging in resource-limited healthcare settings. The initial scarcity of face masks during the early stages of the pandemic led to the development of reusable face mask solutions. This research aimed to design a porous, standalone electrically heatable carbon veil (CV) layer that can be applied to commercial face masks without compromising their breathability. The main objective of this study is to directly inactivate aerosolized viruses using CV heaters powered by a direct current (DC). Prototype face mask samples with the CV were produced and tested using the aerosolized MS2 bacteriophage. After contamination of the face mask with the MS2 bacteriophage, the mask was treated by applying a direct current of 6 V and 1.17 A, which caused the surface temperature of the CV layers to reach over 70 °C within 10 s. This rapid temperature increase through Joule heating effectively inactivates the captured MS2 bacteriophage, with an average inactivation efficiency exceeding 99%. The findings of this study provide valuable insights into the potential application of engineered carbon layers for the decontamination of face masks and air filters from aerosolized viruses, thereby potentially enabling their reuse.

Harmful cyanobacterial blooms (HCBs) are of growing global concern due to their production of toxic compounds, which threaten ecosystems and human health. Saxitoxins (STXs), commonly known as paralytic shellfish poison, are a neurotoxic alkaloid produced by some cyanobacteria. Although many field studies indicate a widespread distribution of STX, it is understudied relative to other cyanotoxins such as microcystins (MCs). In this study, we assessed eleven U.S. urban lakes using qPCR, sxtA gene-targeting sequencing, and 16S rRNA gene sequencing to understand the spatio-temporal variations in cyanobacteria and their potential role in STX production. During the blooms, qPCR analysis confirmed the presence of the STX-encoding gene sxtA at all lakes. In particular, the abundance of the sxtA gene had a strong positive correlation with STX concentrations in Big 11 Lake in Kansas City, which was also the site with the highest quantified STX concentration. Sequencing analysis revealed that potential STX producers, such as Aphanizomenon, Dolichospermum, and Raphidiopsis, were present. Further analysis targeting amplicons of the sxtA gene identified that Aphanizomenon and/or Dolichospermum are the primary STX producer, showing a significant correlation with sxtA gene abundances and STX concentrations. In addition, Aphanizomenon was associated with environmental factors, such as conductivity, sulfate, and orthophosphate, whereas Dolichospermum was correlated with temperature and pH. Overall, the results herein enhance our understanding of the STX-producing cyanobacteria and aid in developing strategies to control HCBs.

Ferrate (Fe(VI): HFeO4- /FeO42-), a potent oxidant, has been investigated as an alternative chemical disinfectant in water treatment due to its reduced production of disinfection by-products. In this study, we assessed the disinfecting ability of potassium ferrate against a variety of microorganisms, including waterborne pathogens, under varying pH and water temperature conditions. We presented contact time (CT) values, which are a metric of ferrate concentrations (C) and CT (T), to quantify microbial inactivation rates. Among the tested microorganisms, human adenovirus was the least resistant to ferrate, followed by waterborne bacteria such as Escherichia coli and Vibrio cholerae, and finally, the protozoan parasite Giardia duodenalis. We further investigated the impact of two pH values (7 and 8) and two temperatures (5 and 25 degrees C) on microbial inactivation rates, observing that inactivation rates increased with lower pH and higher temperature. In addition to showcasing ferrate's capacity to effectively inactivate a range of the tested microorganisms, we offer a ferrate CT table to facilitate the comparison of the effectiveness of various disinfection methods.

Infections of Legionnaires' disease in the United States caused by Legionella have increased ninefold between the years 2000-2018. Legionella harbored in biofilms or inside amoeba within premise plumbing can be more resistant to disinfectants, thus causing treatment challenges. Ultraviolet-light emitting diodes (UV-LEDs) are an emerging water disinfection technology with several advantages over conventional UV lamps. In this study, we evaluated the effects of UV-LEDs (255, 265, and 285 nm), a low-pressure (LP) mercury UV lamp (254 nm), and a bandpass filtered medium-pressure (MP) mercury UV lamp (220 nm) on properties and inactivation of three strains of L. pneumophila serogroup 1. The UV-LEDs emitting at 255 and 265 nm showed greater inactivation performance against all the strains compared to the UV-LED at 285 nm and the LP UV lamp at 254 nm. Our results showed that strains of the same serogroup exhibited different UV sensitivities. Analyses of DNA and protein damage revealed that UV exposure using 254, 255, and 265 nm predominantly causes DNA damage, while protein damage is predominant at 220 nm. Both DNA and protein damage were observed at 285 nm, but the extent of DNA damage was relatively less significant compared to the other wavelengths. Electric energy consumption analysis showed that water treatment using UV-LEDs is currently unsatisfactory compared to conventional LP UV lamps due to the mediocre wall plug efficiency (WPE) of UV-LEDs. However, recent studies indicate that the WPE of UV-LEDs is continuously improving. Overall, our study highlights that UV-LEDs are a promising technology for inactivating waterborne pathogens and have the potential to replace existing UV mercury lamps for water disinfection applications.

The contribution of denitrifying anaerobic methane oxidation (DAMO) as a methane sink across different habitats, especially those affected by anthropogenic activities, remains unclear. Mining and industrial and domestic use of metals/metal-containing compounds can all cause metal contamination in freshwater ecosystems. Precipitation of metal ions often limits their toxicity to local microorganisms, yet microbial activity may also cause the redissolution of various precipitates. In contrast to most other studies that apply soluble metal compounds, this study investigated the responses of enriched DAMO culture to model insoluble copper compounds, malachite and covellite, in simulated sedimentary environments. Copper ≤ 0.22 µm from covellite appeared to cause immediate inhibition in 10 h. Long-term tests (54 days) showed that apparent methane consumption was less impacted by various levels of malachite and covellite than soluble copper. However, the medium-/high-level malachite and covellite caused a 46.6–77.4% decline in denitrification and also induced significant death of the representative DAMO microorganisms. Some enriched species, such as Methylobacter tundripaludum, may have conducted DAMO or they may have oxidized methane aerobically using oxygen released by DAMO bacteria. Quantitative polymerase chain reaction analysis suggests that Candidatus Methanoperedens spp. were less affected by covellite as compared to malachite while Candidatus Methylomirabilis spp. responded similarly to the two compounds. Under the stress induced by copper, DAMO archaea, Planctomycetes spp. or Phenylobacterium spp. synthesized PHA/PHB-like compounds, rendering incomplete methane oxidation. Overall, the findings suggest that while DAMO activity may persist in ecosystems previously exposed to copper pollution, long-term methane abatement capability may be impaired due to a shift of the microbial community or the inhibition of representative DAMO microorganisms.

The biological treatment process consisting of an aerated contactor and filter is effective for groundwaters containing elevated ammonia and other reduced contaminants, including iron, manganese, arsenic, and methane. Depth profiles characterizing microbial activity across aerated contactors are lacking. A 1-year pilot study comparing gravel-and ceramic-packed contactors was conducted, and media depth profile samples were collected at the conclusion of the study. Media and water samples also were collected from pilot-scale aerated contactors at 4 other water systems. Water quality, media surface metals concentrations, and a suite of biofilm parameters were analyzed. Media surface metals concentrations were greatest at the influent end. ATP concentrations, extracellular polymeric substances, and extracellular enzyme activities tended to be similar across depth. Bacteria and functional genes involved in contaminant oxidation co-occurred and tended to decrease across depth, but were not correlated to the media metals concentration. Microbial community composition changed with depth, and the diversity either decreased or remained similar. The microbial activity profiles through aerated contactors differed from what is typically reported for groundwater biofilters, suggesting that the different reactor flow and dissolved oxygen profiles impacted the microbial community.

A Pilot-Scale Study of Flow-through Ultraviolet-Light Emitting Diodes (UV-LEDs) System for Secondary Municipal Wastewater Effluent DisinfectionAbstractThis study investigated the performance of a flow-through UV-LED POE system for disinfecting municipal wastewater secondary effluent. Pilot-scale tests were conducted to determine optimal operating conditions in terms of flow rate and UV wavelength combinations. The inactivation of total coliforms, E. coli, bacteriophage MS2, and HPC bacteria was monitored to evaluate the disinfection efficacy. Results revealed that the inactivation performance was significantly affected by UV transmittance and flow rate. UV wavelength had a minor impact on disinfection performance. The study also estimated the specific energy consumption and cost implications for meeting E. coli limit in wastewater discharge permit. The findings provide valuable insights for the design and optimization of UV-LED POE systems in municipal wastewater treatment, emphasizing the importance of hydraulic residence time, UV wavelength selection, and energy efficiency considerations.This study investigated the performance of a flow-through UV-LED POE system for disinfecting municipal wastewater secondary effluent. Optimal operating conditions and the influence of UVT, flow rate, and UV wavelength on the disinfection efficacy were identified through inactivation experiments against total coliforms, E. coli, bacteriophage MS2, and HPC bacteria. The estimated specific energy consumption and cost implications were also evaluated.SpeakerOh, YoontaekPresentation time09:30:0010:00:00Session time08:30:0010:00:00SessionUV Disinfection: Where are we? Where are we going?Session locationRoom S404c - Level 4TopicDisinfection and Public Health, Intermediate Level, Municipal Wastewater Treatment Design, Research and InnovationTopicDisinfection and Public Health, Intermediate Level, Municipal Wastewater Treatment Design, Research and InnovationAuthor(s)Oh, YoontaekAuthor(s)Y. Oh 1; H. Kim 2 ; Y. Oh 1; L. Boczek 3; H. Ryu 4;Author affiliation(s)Pegasus Technical Services, Inc. 1; Research Institute for Advanced Industrial Technology, College of Science and Technology, Korea University 2 ; Pegasus Technical Services, Inc., 26 West Martin Luther King Drive, Cincinnati, OH 1; U.S. Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, OH 3; U.S. Environmental Protection Agency, 26 West Martin Luther King Drive, Cincinnati, OH 4;SourceProceedings of the Water Environment FederationDocument typeConference PaperPublisherWater Environment FederationPrint publication date Oct 2023DOI10.2175/193864718825159163Volume / Issue Content sourceWEFTECCopyright2023Word count17

Aerobic biotreatment systems can treat multiple reduced inorganic contaminants in groundwater, including ammonia (NH3), arsenic (As), iron (Fe), and manganese (Mn). While individual systems treating multiple contaminants simultaneously have been characterized and several systems treating one contaminant have been compared, a comparison of systems treating co-occurring contaminants is lacking. This study assessed the treatment performance and microbial communities within 7 pilot- and full-scale groundwater biotreatment systems in the United States that treated waters with pH 5.6-7.8, 0.1-2.0 mg/L dissolved oxygen, 75-376 mg CaCO3/L alkalinity, < 0.03-3.79 mg NH3-N/L, < 4-31 mu g As/L, < 0.01-9.37 mg Fe/L, 2-1220 mu g Mn/L, and 0.1-5.6 mg/L total organic carbon (TOC). Different reactor configurations and media types were represented, allowing for a broad assessment of linkages between water quality and microbial communities via microscopy, biofilm quantification, and molecular methods. Influent NH3, TOC, and pH contributed to differences in the microbial communities. Mn oxidase gene copy numbers were slightly negatively correlated with the influent Mn concentration, but no significant relationships between gene copy number and influent concentration were observed for the other contaminants. Extracellular enzyme activities, community composition, and carbon transformation pathways suggested heterotrophic bacteria may be important in nitrifying biofilters. Aerobic groundwater biofilters are complex, and improved understanding could lead to engineering enhancements.

Opportunistic premise plumbing pathogens (OPPPs) have been detected in buildings' plumbing systems causing waterborne disease outbreaks in the United States. In this study, we monitored the occurrence of OPPPs along with free-living amoeba (FLA) and investigated the effects of residential activities in a simulated home plumbing system (HPS). Water samples were collected from various locations in the HPS and analyzed for three major OPPPs: Legionella pneumophila, nontuberculous mycobacterial species (e.g., Mycobacterium avium, M. intracellulare, and M. abscessus), and Pseudomonas aeruginosa along with two groups of amoebas (Acanthamoeba and Vermamoeba vermiformis). A metagenomic approach was also used to further characterize the microbial communities. Results show that the microbial community is highly diverse with evidence of spatial and temporal structuring influenced by environmental conditions. L. pneumophila was the most prevalent pathogen (86% of samples), followed by M. intracellulare (66%) and P. aeruginosa (21%). Interestingly, M. avium and M. abscessus were not detected in any samples. The data revealed a relatively low prevalence of Acanthamoeba spp. (4%), while V. vermiformis was widely detected (81%) across all the sampling locations within the HPS. Locations with a high concentration of L. pneumophila and M. intracellulare coincided with the highest detection of V. vermiformis, suggesting the potential growth of both populations within FLA and additional protection in drinking water. After a period of stagnation lasting at least 2-weeks, the concentrations of OPPPs and amoeba immediately increased and then decreased gradually back to the baseline. Furthermore, monitoring the microbial population after drainage of the hot water tank and partial drainage of the entire HPS demonstrated no significant mitigation of the selected OPPPs. This study demonstrates that these organisms can adjust to their environment during such events and may survive in biofilms and/or grow within FLA, protecting them from stressors in the supplied water.

In this study, a novel molybdenum disulfide (MoS2) nano-carbon (NC) coated cathode was developed for hydrogen production in a microbial electrolysis cell (MEC), while treating simulated urine with 2-6 times dilution (conductivity <20 mS cm-1). MoS2 nanoparticles were electrodeposited on the NC coated cathodes at -100, -150 and -200 μA cm-2 and their performances were evaluated in the MEC. The chronopotentiometry (CP) tests showed the improved catalytic activity of MoS2-NC cathodes with much lower cathode overpotential than non-MoS2 coated electrodes. The MoS2-NC200 cathode, electrodeposited at -200 μA cm-2, showed the maximum hydrogen production rate of 0.152 ± 0.002 m3 H2 m-2 d-1 at 0.9V of Eap, which is comparable to the previously reported Pt electrodes. It was found that high solution conductivity over 20 mS cm-1 (>600 mg L-1 NH3-N) can adversely affect the biofilm architecture and the bacterial activity at the anode of the MEC. Exoelectrogenic bacteria for this system at the anode were identified as Tissierella (Clostridia) and Bacteroidetes taxa. Maximum ammonia-nitrogen (NH3-N) and phosphorus (PO43--P) removal were 68.7 and 98.6%, respectively. This study showed that the newly fabricated MoS2-NC cathode can be a cost-effective alternative to the Pt cathode for renewable bioelectrochemical hydrogen production from urine.

Center for Environmental Measurement & Modeling, United States Environmental Protection Agency, Cincinnati, OH, United States, 2 Santa Ana Regional Water Quality Control Board, California Environmental Protection Agency, Riverside, CA, United States, Center for Environmental Solutions and Emergency Response, United States Environmental Protection Agency, Cincinnati, OH, United States, Division of Biological Sciences, STEM, University of Washington, Bothell, WA, United States

Background Rivers and lakes are used for multiple purposes such as for drinking water (DW) production, recreation, and as recipients of wastewater from various sources. The deterioration of surface water quality with wastewater is well-known, but less is known about the bacterial community dynamics in the affected surface waters. Understanding the bacterial community characteristics —from the source of contamination, through the watershed to the DW production process—may help safeguard human health and the environment. Results The spatial and seasonal dynamics of bacterial communities, their predicted functions, and potential health-related bacterial (PHRB) reads within the Kokemäenjoki River watershed in southwest Finland were analyzed with the 16S rRNA-gene amplicon sequencing method. Water samples were collected from various sampling points of the watershed, from its major pollution sources (sewage influent and effluent, industrial effluent, mine runoff) and different stages of the DW treatment process (pre-treatment, groundwater observation well, DW production well) by using the river water as raw water with an artificial groundwater recharge (AGR). The beta-diversity analysis revealed that bacterial communities were highly varied among sample groups (R = 0.92, p < 0.001, ANOSIM). The species richness and evenness indices were highest in surface water (Chao1; 920 ± 10) among sample groups and gradually decreased during the DW treatment process (DW production well; Chao1: 320 ± 20). Although the phylum Proteobacteria was omnipresent, its relative abundance was higher in sewage and industrial effluents (66–80%) than in surface water (55%). Phyla Firmicutes and Fusobacteria were only detected in sewage samples. Actinobacteria was more abundant in the surface water (≥13%) than in other groups (≤3%). Acidobacteria was more abundant in the DW treatment process (≥13%) than in others (≤2%). In total, the share of PHRB reads was higher in sewage and surface water than in the DW treatment samples. The seasonal effect in bacterial communities was observed only on surface water samples, with the lowest diversity during summer. Conclusions The low bacterial diversity and absence of PHRB read in the DW samples indicate AGR can produce biologically stable and microbiologically safe drinking water. Furthermore, the significantly different bacterial communities at the pollution sources compared to surface water and DW samples highlight the importance of effective wastewater treatment for protecting the environment and human health.

Microbial fuel cells (MFCs) have recently been applied to generate electricity from oily wastewater. Although MFCs that utilize microalgae to provide a self-supporting oxygen (O2) supply at the cathode have been well discussed, those with microalgae at the anode as an active biomass for treating wastewater and producing electrons are still poorly studied and understood. Here, we demonstrated a bilgewater treatment using single- and double-chamber microalgal fuel cells (SMAFC and DMAFC) capable of generating energy with a novel microalgal strain (Chlorella sorokiniana) that was initially isolated from oily wastewater. Compared to previous MFC studies using green algae, relatively high voltage output (151.3-160.1 mV, 71.3-83.4 mV m-2 of power density) was observed in the SMAFC under O2 controlled systems (i.e., acetate addition or light/dark cycle). It was assumed that, under the O2 depletion, alternative electron acceptors such as bicarbonate may be utilized for power generation. A DMAFC showed better power density (up to 23.9%) compared to the SMAFC due to the separated cathode chamber which fully utilizes O2 as an electron acceptor. Both SMAFC and DMAFC removed 67.2-77.4% of soluble chemical oxygen demands (SCOD) from the synthetic bilgewater. This study demonstrates that the application of algae-based MFCs is a feasible strategy to treat oil-in-water emulsion while generating electricity.

A proof-of-concept study evaluates the performance of a novel strategy using photosynthetic microorganisms to soften groundwater instead of using caustic chemicals. The microalga Scenedesmus quadricauda was used to increase the pH of the groundwater via natural photosynthesis. This work applied softening as a pretreatment to ozonation of hard groundwater and mainly focused on investigating the multiple effects of algal softening on the degradation of persistent micropollutants upon subsequent ozonation. The algae-induced alkaline conditions (pH > 10) were favorable to catalyze the formation of OH radicals directly from O-3 molecules. Moreover, algal softening removed the strong radical-scavenging carbonate species (HCO3- and CO32-) to a much greater extent than that achieved by chemical softening, which was attributed to the combination of mineral carbonation and metabolic CO2 reduction. The fate of the natural organic matter (NOM) was characterized with spectroscopy, chromatography, and bioassay, which indicates that algal treatment decomposed the NOM to be less susceptible to attack by OH radicals. Consequently, the ozonation of alkaline groundwater achieved a better removal of the micropollutant residues in groundwater. Carbamazepine and diclofenac were used as model chemicals of persistent groundwater contaminants and were almost completely removed with an addition of 1.25 mg O-3 L-1 (0.63 mg-O-3 mg-C-1).

Legionella infections have steadily increased in the United States over the last 20 years, and most of these infections have been attributed to contaminated water. The gold standard for confirmation of Legionella presence in water is culturing with Buffered Charcoal Yeast Extract (BCYE) agar. Following many modifications, this method is still time-consuming, expensive, and can take longer than 10 days for full confirmation. The Legiolert is a newer and simpler culture product that is claimed to be able to quantify Legionella pneumophila in 7 days with high sensitivity and specificity and does not need further confirmation for the presence of L. pneumophila. This study compared the culturability of L. pneumophila occurring in a simulated home plumbing system using both Legiolert and BCYE agar methods. Out of 185 water samples, Legiolert and BCYE method detected L. pneumophila in 83 and 85% of the samples, respectively. The two methods were determined to be statistically equivalent for culturability of L. pneumophila, though the detected levels by Legiolert were slightly higher than the BCYE method. The molecular confirmation of positive (n = 254) and negative wells (n = 82) with Legiolert also showed a high specificity of 96.5% (i.e., 3.5% false positives (9/254) and 0% false negatives (0/82)).