Immunohistochemistry is an indispensable tool in human pathology enabling immunophenotypic characterization of tumor cells. Immunohistochemical analyses of mouse models of human hematopoietic neoplasias have become an important aspect for comparison of murine entities with their human counterparts. The aim of this study was to establish a diagnostic antibody panel for analysis of murine lymphomas/leukemias, useful in formalin-fixed/paraffin-embedded tissue. Overall, 48 antibodies (4 rabbit monoclonal, 12 rabbit polyclonal, 2 goat polyclonal, 11 rat, and 19 mouse monoclonal), which were either mouse-specific (14) or cross-reactive with murine tissue (34) were tested for staining quality and diagnostic value in 468 murine hematopoietic neoplasms. Specific staining was achieved with 29 antibodies, of which 18 were human antibodies cross-reactive with murine tissue. only 23 (B220, BCL-2, BCL-6, CD117, CD138, (2x), CD3 (2x), CD43. CD45, CD5, CD79 alpha cy, cyclin D1, Ki-67 (2x), Mac-3, Mac-2, lysozyme, mast cell tryptase, MPO, Pax-5, TdT, and TER-119) were regarded as valuable for diagnostic evaluation. Immunohistochemistry was also established in an automated immunostainer for high throughput analysis. The antibody panel developed is useful for the classification of murine lymphomas and leukemias analyzed, and a valuable tool for human and veterinary pathologists involved in the diagnostic interpretation of murine models of hematopoietic neoplasias.

After horseradish peroxidase was injected into the rat pineal gland, isolated and rare labelled perikarya appeared in the central nervous system, particularly in the habenula, colliculi, amygdala, paraventricular and suprachiasmatic nuclei, preoptic area and olfactive centers. This indicates that the innervation of the pineal gland is not only orthosympathetic but that some stimuli can be directly transmitted from the brain to the pineal stalk.

Mono- and oligospecific lymphocytotoxic alloantibodies from primiparous mares were tested on cells from horse families of various breeds in the two-step microcytotoxicity assay. The results showed that the detected antigens were inherited co-dominantly and autosomally as simple Mendelian traits. The membrane antigens showed different linkage with one or more other antigens and seem to be coded by a limited number of loci (at least three) from one chromosome. In the families tested one recombinant for the serologically defined antigens was recognized. The mixed leukocyte reactions of cells from horse families compared with the serologically recognized antigens showed that the two systems are inherited with the same chromosome. A homozygote for both antigen systems was recognized in a family.

In electron micrographs of serial sections of a rat juxtaglomerular apparatus "axon segments" were analyzed for contact with the cells of the vascular component and surrounding tubules. Slightly less than one-fourth of the cells of the vascular component were innervated. Nerve endings were seen on less than one-third of the cells of the afferent arteriole and about one-third of the cells of the efferent arteriole. The mesangial region had only 3 of its 30 cells innervated. Of these 3 cells, 2 were in contact with the distal tubule. The majority of the innervated cells were contacted by nerve endings belonging to more than one axon segment. Frequently en passant nerve endings from the same axon were seen to contact several different cells (granular and agranular) of the vascular component. Individual axons also established en passant contact with granular and agranular cells of the vascular component and tubular cells. These findings support the view that the neural effect on renin secretion is t the result of a complex response to neural activity which includes, besides direct action on granular cells, indirect action mediated through its effect on arteriolar and renal tubular systems.

An innervation of the tubules of the monkey renal cortex has been demonstrated by electron microscopy. At the light microscopic level, histochemical methods showed the existence of both adrenergic and cholinergic nerve fibers in association with the renal tubules. By electron microscopy, bundles of terminal varicose axons were observed traveling between the blood vessel and tubules. Nerve endings in these bundles established contact with the basement membranes of both proximal and distal tubules as well as smooth muscle cells of the arterioles. Small bundles of axons having little or no Schwann cell covering were seen running between the tubules. Varicosities of these axons make contact with proximal and distal tubular cell basement membranes. By light microscopy adrenergic and cholinergic terminal axons were seen in close association with proximal and distal tubules. Serial sections showed that the tubular nerve endings are part of the perivascular nerve bundles or originate from them and travel independently for variable distances among the tubules. These studies provide an anatomical basis for a direct action of the autonomic nervous system on renal tubular function.