Wheat immunity against the global pest Hessian fly operates on a gene-for-gene basis, yet molecular mechanisms underlying resistance-avirulence interactions have remained elusive for over five decades due to the absence of any cloned insect-resistance gene. Here, we cloned the H13 resistance gene encoding a coiled-coil/nucleotide-binding/leucine-rich repeat protein that protects wheat against multiple Hessian fly biotypes, and demonstrate direct molecular interaction with its cognate avirulence effector, vH13. H13-vH13 recognition activates effector-triggered immunity through calcium signaling, oxidative burst, jasmonic acid and ethylene, but not salicylic acid, signaling pathways, leading to larval antibiosis. Notably, H13 is evolutionarily unique, lacking similarity with other wheat disease- or plant insect-resistance proteins. This study provides a mechanistic model for understanding and engineering durable insect resistance in crops.

The Deep Space Atomic Clock (DSAC), a NASA Technology Demonstration Mission, was launched into low-Earth orbit on June 25, 2019 as a hosted payload aboard General Atomics' Orbital Test Bed (OTB) spacecraft. The DSAC mission has been conducting a two-year demonstration of a mercury ion atomic clock to characterize its space-based performance and to validate its utility for deep space navigation and radio science. Analysis of the collected data using JPL's GIPSY-OASIS software has shown DSAC's Allan Deviation (AD) at one-day to be near 3×10 -15 ; much better than required AD of 2×10 -14 . Such low spacecraft clock errors will enable one-way radiometric tracking data with precision equivalent to or better than current-day two way tracking data, allowing a shift to a more efficient and flexible one-way deep space navigation architecture. To verify this, an analog deep space navigation experiment was performed using JPL's operational navigation software (Monte). The experiment recovered orbit solutions with reduced data sets and geometric variations that are more representative of deep space missions, and showed that orbit determination using DSAC derived data is on par with more traditional two-way datatypes. As a technology demonstrator, DSAC's development focus has been on maturing the mercury ion trap clock technology rather than achieving the smallest size, weight, and power (SWaP). Over the course of DSAC's development the project has identified numerous improvements that could be made to significantly reduce SWaP for DSAC's next version. Indeed, DSAC-2 was recently selected by NASA for further demonstration on the VERITAS mission to Venus. This work will review the DSAC technology, mission, and results from its two-year mission.

The timing and frequency stability provided by the Deep Space Atomic Clock (DSAC) is nearly commensurate to the Deep Space Network's ground clocks and enables one-way radiometric measurements with accuracy equivalent to current two-way tracking data. A demonstration unit of the clock was launched into low Earth orbit on June 25, 2019, for the purpose of validating DSAC's performance in the space environment. Global Positioning System ( GPS) data collected throughout the two-year mission was utilized not only for precise clock estimation but also as a proxy for deep space tracking data to conduct the Deep Space Navigation Analog Experiment. Through careful selection and processing of GPS Doppler data and limited modeling fidelity representative of deep space navigation capabilities, the analog orbit solutions are compared to higher-fidelity solutions, demonstratingDSAC's viability as a navigation instrument in conditions typical for a low-altitude Mars orbiter. Onboard telemetry quantifying the ultrastable oscillator (USO) frequency correction is processed to demonstrate the orbit determination performance degradation when utilizing USO-based one-way radiometric tracking data.

Placing a small satellite into a high-inclination orbit with respect to the ecliptic plane may offer a low-cost option for opportunistic and targeted observations of the polar regions of the Sun or the zodiacal dust cloud of the solar system. In this paper, dynamical systems theory and hybrid optimization techniques are integrated into a cohesive framework to design low-thrust trajectories for a small satellite to reach a highly out-of-ecliptic science orbit near the Sun-Earth L-2 equilibrium point. Propellant-optimal low-thrust trajectories with a specific geometry are designed and studied across a variety of engine and power models within a low-thrust-enabled circular restricted three-body problem. The geometry of the trajectories is then varied during the initial guess construction process to support a preliminary study of the tradeoff between flight time and propellant mass usage.

A growing interest in small body exploration has motivated research into the rapid characterization of near-Earth objects to meet economic or scientific objectives. Specifically, knowledge of the internal density structure can aid with target selection and enables an understanding of prehistoric planetary formation to be developed. To this end, multi-layer extensions to the polyhedral gravity model are suggested, and an inversion technique is implemented to present their effectiveness. On-orbit gravity gradiometry is simulated and employed in stochastic and deterministic algorithms, with results that imply robustness in both cases.

Coffee berry borer-CBB (Hypothenemus hampei) is a globally important economic pest of coffee (Coffea spp.). Despite current insect control methods for managing CBB, development of future control strategies requires a better understanding of its biology and interaction with its host plant. Towards this objective, we performed de novo CBB genome and transcriptome sequencing, improved CBB genome assembly and predicted 18,765 protein-encoding genes. Using genome and transcriptome data, we annotated the genes associated with chemosensation and found a reduced gene repertoire composed by 67 odorant receptors (ORs), 62 gustatory receptors (GRs), 33 ionotropic receptors (IRs) and 29 odorant-binding proteins (OBPs). In silico transcript abundance analysis of these chemosensory genes revealed expression enrichment in CBB adults compared with larva. Detection of differentially expressed chemosensory genes between males and females is likely associated with differences in host-finding behavior between sexes. Additionally, we discovered male-specific genome content and identified candidate male-specific expressed genes on these scaffolds, suggesting that a Y-like chromosome may be involved in the CBB's functional haplodiploid mechanism of sex determination.

Exoplanet discoveries since the mid-1990's have revealed an astounding diversity of planetary systems. Studying these systems is essential to understanding planetary formation processes, as well as the development of life in the universe. Unfortunately, humanity can only observe limited aspects of exoplanetary systems by telescope, and the significant distances between stars presents a barrier to in situ exploration. In this study, we propose an alternative path to gain insight into exoplanetary systems: Bridge, a mission concept design to fly by an interstellar object as it passes through our solar system. Designed as a New Frontiers-class mission during the National Aeronautics and Space Administration (NASA) Planetary Science Summer Seminar, Bridge would provide a unique opportunity to gain insight into potential physical, chemical, and biological differences between solar systems as well as the possible exchange of planetary materials between them. Bridge employs ultraviolet/visible, near-infrared, and mid-infrared point spectrometers, a visible camera, and a guided impactor. We also provide a quantitative Monte Carlo analysis that estimates wait times for a suitable target, and examines key trades between ground storage and a parking orbit, power sources, inner versus outer solar system encounters, and launch criteria. Due to the fleeting nature of interstellar objects, reaching an interstellar object may require an extended ground storage phase for the spacecraft until a suitable target is discovered, followed by a rapid response launch strategy. To enable rapid response missions designed to intercept such unique targets, language would need to be added to future NASA announcements of opportunity such that ground storage and rapid response would be allowable components of a proposed mission.

The Hessian fly (HF, Mayetiola destructor) is a plant-galling parasite of wheat (Triticum spp.). Seven percent of its genome is composed of highly diversified signal-peptide-encoding genes that are transcribed in HF larval salivary glands. These observations suggest that they encode effector proteins that are injected into wheat cells to suppress basal wheat immunity and redirect wheat development towards gall formation. Genetic mapping has determined that mutations in four of these genes are associated with HF larval survival (virulence) on plants carrying four different resistance (R) genes. Here, this line of investigation was pursued further using bulked-segregant analysis combined with whole genome resequencing (BSA-seq). Virulence to wheat R genes H6, Hdic, and H5 was examined. Mutations associated with H6 virulence had been mapped previously. Therefore, we used H6 to test the capacity of BSA-seq to map virulence using a field-derived HF population. This was the first time a non-structured HF population had been used to map HF virulence. Hdic virulence had not been mapped previously. Using a structured laboratory population, BSA-seq associated Hdic virulence with mutations in two candidate effector-encoding genes. Using a laboratory population, H5 virulence was previously positioned in a region spanning the centromere of HF autosome 2. BSA-seq resolved H5 virulence to a 1.3 Mb fragment on the same chromosome but failed to identify candidate mutations. Map-based candidate effectors were then delivered to Nicotiana plant cells via the type III secretion system of Burkholderia glumae bacteria. These experiments demonstrated that the genes associated with virulence to wheat R genes H6 and H13 are capable of suppressing plant immunity. Results are consistent with the hypothesis that effector proteins underlie the ability of HFs to survive on wheat.

Insect herbivores can manipulate host plants to inhibit defenses. Insects that induce plant galls are excellent examples of these interactions. The Hessian fly (HF, Mayetiola destructor) is a destructive pest of wheat (Triticum spp.) that occurs in nearly all wheat producing globally. Under compatible interactions (i.e., successful HF establishment), HF larvae alter host tissue physiology and morphology for their benefit, manifesting as the development of plant nutritive tissue that feeds the larva and ceases plant cell division and elongation. Under incompatible interactions (i.e., unsuccessful HF establishment), plants respond to larval feeding by killing the larva, permitting normal plant development. We used reflectance spectroscopy to characterize whole-plant functional trait responses during both compatible and incompatible interactions and related these findings with morphological and gene expression observations from earlier studies. Spectral models successfully characterized wheat foliar traits, with mean goodness of fit statistics of 0.84, 0.85, 0.94, and 0.69 and percent root mean square errors of 22, 10, 6, and 20%, respectively, for nitrogen and carbon concentrations, leaf mass per area, and total phenolic content. We found that larvae capable of generating compatible interactions successfully manipulated host plant chemical and morphological composition to create a more hospitable environment. Incompatible interactions resulted in lower host plant nutritional quality, thicker leaves, and higher phenolic levels. Spectral measurements successfully characterized wheat responses to compatible and incompatible interactions, providing an excellent example of the utility of Spectral phenotyping in quantifying responses of specific plant functional traits associated with insect resistance.

Several mission concepts entail the placement of a spacecraft into a high inclination orbit with respect to the ecliptic plane. Among these mission concepts are solar observatories targeting the polar regions of the Sun or spacecraft seeking an external vantage point on the zodiacal dust cloud of our solar system. In this investigation, techniques for low-thrust and low-energy trajectory design will be integrated into a cohesive framework to access these highly out-of-ecliptic science orbits. The focus of this investigation will be on spacecraft conforming to a SmallSat form-factor, enabling opportunistic science as secondary payloads or via smaller launch vehicles.

Asteroids are of particular interest in the modem space industry due to their potential for advancing knowledge about the origins of the solar system. Identifying and characterizing asteroids with sustainable resources for future space exploration is critical. Additionally, limited knowledge of near-Earth asteroids' physical characteristics such as shape, density, gravity field, and composition pose a challenge to any manned exploration. A stochastic gradient method from the field of deep learning is applied to the problem of asteroid density recovery, with implications for target selection and mining. The algorithm outperforms the predicted observability, and is minimally affected by noisy measurements.

Con el objetivo de encontrar nuevas fuentes de resistencia a la enfermedad de la hoja blanca del arroz, transmitida por el insecto Tagosodes orizicolus, se evaluaron 660 genotipos en condiciones de invernadero y campo. Se identificaron siete genotipos con resistencia a la enfermedad y se realizaron estudios del genoma para evidenciar que eran genéticamente diferentes a Fedearroz 2000, la variedad de mejor comportamiento ante el virus, en el momento. Estas nuevas fuentes de resistencia constituyen un recurso que puede utilizarse para extender un manejo sostenible de la enfermedad, en todas las regiones productoras de arroz en América tropical. Este es el primer reporte de fuentes de resistencia, tipo indica, diferentes a Fedearroz 2000.

Spacecraft formations enable a variety of mission concepts, from gravity reconstruction to extrasolar planet detection to heliophysics observatories. In particular, space-based radio interferometers can detect signals in frequency ranges that are absorbed by Earth's ionosphere or atmosphere. Furthermore, such formation missions are now feasible under constrained cost caps due to the growing availability of SmallSat components with spaceflight heritage that are compatible the CubeSat form-factor. Accordingly, we analyze a mission concept operating multiple SmallSats in a passive cluster to establish a space-based interferometer. The goal of this array is to reconstruct radio emissions associated with Coronal Mass Ejections (CMEs) from the Sun, giving us insight into the particle acceleration occurring during these events. This low-cost mission concept presents a number of unique challenges in regards to mission design and navigation, particularly the configuration of the spacecraft to optimize science return and the reconstruction of precise spacecraft-spacecraft separation values. After providing a brief overview of the mission concept, this paper presents several key features of the mission design and orbit determination strategy that enable this potential heliophysics mission.

Spacecraft component miniaturization, the standardized CubeSat form factor, and a corresponding increase in launch ride-share availability have led to a renewed interest in smaller, more agile spacecraft missions. Naturally, interest in SmallSat capabilities extends outside of low Earth orbit for a variety of science and technology demonstration applications. As with larger missions, near-term deep-space SmallSats will more than likely rely on telecommunications and tracking via NASA’s Deep Space Network or similar facilities. Given the predicted growth in the number of deep space missions, effective use of telecommunication resources will be more critical than ever, even with Multiple Spacecraft Per Aperture capability. In particular, SmallSat missions will likely face stricter limitations on two-way contact with the tracking stations, thus making other options like one-way data types more attractive. While all missions must eventually develop their own detailed navigation plans, a common set of references is needed to support SmallSat missions, especially in the early development phases. Our investigation provides this initial survey of expected navigation performance for standard radiometric data types, from traditional two-way Doppler and ranging capabilities to one-way equivalents, including delta-differential one-way range. In this investigation, we examine transport within the Earth-Moon region, the first step outward from Earth into deep space exploration.

Mayetiola destructor (Say) is a serious pest of wheat, Triticum aestivum L., in North America, North Africa, and Central Asia. Singly deployed resistance genes in wheat cultivars have provided effective management of Hessian fly populations for > 50 yr. Thirty-five H genes have been documented. Defense mediated by the H gene constitutes strong selection on the Hessian fly population, killing 100% of larvae. A mutation in a matching Hessian fly avirulence gene confers virulence to the H gene, leading to survival on the resistant plant. As the frequency of virulence rises in the population, the H gene loses its effectiveness for pest management. Knowing the frequency of virulence in the population is not only important for monitoring but also for decisions about which H gene should be deployed in regional wheat breeding programs. Here, we present a novel assay for detecting virulence in the field. Hessian fly males were collected in Alabama, Georgia, North Carolina, and South Carolina using sticky traps baited with Hessian fly sex pheromone. Utilizing two PCR reactions, diagnostic molecular markers for the six alleles controlling avirulence and virulence to H13 can be scored based on band size. Throughout the southeast, all three avirulence and three virulence alleles can be identified. In South Carolina, the PCR assay was sensitive enough to detect the spread of virulence into two counties previously documented as 100% susceptible to H13. The new assay also indicates that the previous methods overestimated virulence in the field owing to scoring of the plant instead of the insect.

A robotic mission to the Martian moons Phobos and Deimos would offer a wealth of scientific information and serve as a useful precursor to potential human missions. In this paper, we investigate a prospective mission enabled by solar electric propulsion that would explore Phobos via a series of flybys followed by capture into orbit around the moon. Of particular interest are low 6.7 options for capture and walkdown to the target science orbits aided by multi-body effects due to the mutual gravitational interaction of Phobos and Mars. We also consider contingency operations in the event of missed thrust or maneuver execution errors.

The growth in the number of defunct and fragmented objects near to the Earth poses a growing hazard to launch operations as well as existing on-orbit assets. Numerous studies have demonstrated the positive impact of active debris mitigation campaigns upon the growth of debris populations, but comparatively fewer investigations incorporate specific mission scenarios. Furthermore, while many active mitigation methods have been proposed, certain classes of debris objects are amenable to mitigation campaigns employing chaser spacecraft with existing chemical and low-thrust propulsive technologies. This investigation incorporates an ant colony optimization routing algorithm and multi-agent coordination via auctions into a debris mitigation tour scheme suitable for preliminary mission design and analysis as well as spacecraft flight operations. (C) 2015 COSPAR. Published by Elsevier Ltd. All rights reserved.