BACKGROUND STUDY DESIGN AND METHODS Partial D status is a major concern for transfusion and pregnancy, due to the possibility of carriers becoming immunized. When known carriers of a D variant have never been exposed to complete D, they are assumed to have D partial status based on the position of the amino acid substituted. New approaches for predicting immunization risk are required. We built a three-dimensional (3D) structural model to investigate the consequences of substitutions of Amino Acid 223 involved in a large number of D variants. Homology modeling was performed with multiple templates. The model was evaluated by comparing the interactions of the known p.Phe223Val variant (RHD*08.01) and a new p.Phe223Ser variant (RHD*52) to RhD reference allele (p.Phe223). The consequences predicted by modeling the variants were compared with serologic data. RESULTS CONCLUSION The 3D structural model was generated from two related protein structures and assessed with state-of-the-art approaches. An analysis of the interactions of the variant Residue 223 in the proposed 3D model highlighted the importance of this position. Modeling predictions were consistent with the serologic and clinical data obtained for the D antigen with a substitution of Amino Acid 223. We used a 3D structural model to evaluate the effect of the p.Phe223 substitution on the conformation of the RhD protein. This model shed light on the influence of substitutions on the structure of the RhD protein and the associated alloimmunization risk. These initial findings indicate that the p.Phe223Ser variant can be considered partial.

TransfusionVolume 57, Issue 4 p. 1089-1091 REPORT OF NEW ALLELES OR ANTIGENS Molecular characterization of 13 new RHD alleles Aline Floch, Aline Floch Etablissement Français du Sang Île de France Institut National de la Santé et de la Recherche Médicale (INSERM)-U955, Equipe 2: Transfusion et Maladies du Globule Rouge, Institut Mondor de Recherche Biomédicale, Créteil, France Laboratoire d'Excellence GRex, Paris, France Université Paris Est-Créteil, Créteil, FranceSearch for more papers by this authorAurelie Barrault, Aurelie Barrault Etablissement Français du Sang Île de FranceSearch for more papers by this authorAlexandre G. De Brevern, Alexandre G. De Brevern orcid.org/0000-0001-7112-5626 Laboratoire d'Excellence GRex, Paris, France INSERM U1134, Dynamique des Structures et Interactions des Macromolécles Biologiques INSERM UMR_S 1134, Université Paris Diderot, Sorbonne Paris Cité Institut National de la Transfusion Sanguine, Paris, FranceSearch for more papers by this authorJennifer Martret, Jennifer Martret Etablissement Français du Sang Île de FranceSearch for more papers by this authorKevin Gaillard, Kevin Gaillard Etablissement Français du Sang Île de FranceSearch for more papers by this authorLaurent Devaux, Laurent Devaux Etablissement Français du Sang Île de FranceSearch for more papers by this authorBtissam Chami, Btissam Chami Etablissement Français du Sang Île de FranceSearch for more papers by this authorPhilippe Bierling, Philippe Bierling Etablissement Français du Sang Île de France Institut National de la Santé et de la Recherche Médicale (INSERM)-U955, Equipe 2: Transfusion et Maladies du Globule Rouge, Institut Mondor de Recherche Biomédicale, Créteil, France Laboratoire d'Excellence GRex, Paris, France Université Paris Est-Créteil, Créteil, FranceSearch for more papers by this authorFrance Pirenne, France Pirenne Etablissement Français du Sang Île de France Institut National de la Santé et de la Recherche Médicale (INSERM)-U955, Equipe 2: Transfusion et Maladies du Globule Rouge, Institut Mondor de Recherche Biomédicale, Créteil, France Laboratoire d'Excellence GRex, Paris, France Université Paris Est-Créteil, Créteil, FranceSearch for more papers by this authorChristophe Tournamille, Corresponding Author Christophe Tournamille christophe.tournamille@efs.sante.fr Etablissement Français du Sang Île de France Institut National de la Santé et de la Recherche Médicale (INSERM)-U955, Equipe 2: Transfusion et Maladies du Globule Rouge, Institut Mondor de Recherche Biomédicale, Créteil, France Laboratoire d'Excellence GRex, Paris, FranceAddress reprint requests to: Christophe Tournamille, Laboratoire IHM, Etablissement Français du Sang, Bâtiment UITC, Centre Félix Reyes, 2e étage, 5 rue Gustave Eiffel, 94000 Créteil, France; e-mail: christophe.tournamille@efs.sante.fr.Search for more papers by this author Aline Floch, Aline Floch Etablissement Français du Sang Île de France Institut National de la Santé et de la Recherche Médicale (INSERM)-U955, Equipe 2: Transfusion et Maladies du Globule Rouge, Institut Mondor de Recherche Biomédicale, Créteil, France Laboratoire d'Excellence GRex, Paris, France Université Paris Est-Créteil, Créteil, FranceSearch for more papers by this authorAurelie Barrault, Aurelie Barrault Etablissement Français du Sang Île de FranceSearch for more papers by this authorAlexandre G. De Brevern, Alexandre G. De Brevern orcid.org/0000-0001-7112-5626 Laboratoire d'Excellence GRex, Paris, France INSERM U1134, Dynamique des Structures et Interactions des Macromolécles Biologiques INSERM UMR_S 1134, Université Paris Diderot, Sorbonne Paris Cité Institut National de la Transfusion Sanguine, Paris, FranceSearch for more papers by this authorJennifer Martret, Jennifer Martret Etablissement Français du Sang Île de FranceSearch for more papers by this authorKevin Gaillard, Kevin Gaillard Etablissement Français du Sang Île de FranceSearch for more papers by this authorLaurent Devaux, Laurent Devaux Etablissement Français du Sang Île de FranceSearch for more papers by this authorBtissam Chami, Btissam Chami Etablissement Français du Sang Île de FranceSearch for more papers by this authorPhilippe Bierling, Philippe Bierling Etablissement Français du Sang Île de France Institut National de la Santé et de la Recherche Médicale (INSERM)-U955, Equipe 2: Transfusion et Maladies du Globule Rouge, Institut Mondor de Recherche Biomédicale, Créteil, France Laboratoire d'Excellence GRex, Paris, France Université Paris Est-Créteil, Créteil, FranceSearch for more papers by this authorFrance Pirenne, France Pirenne Etablissement Français du Sang Île de France Institut National de la Santé et de la Recherche Médicale (INSERM)-U955, Equipe 2: Transfusion et Maladies du Globule Rouge, Institut Mondor de Recherche Biomédicale, Créteil, France Laboratoire d'Excellence GRex, Paris, France Université Paris Est-Créteil, Créteil, FranceSearch for more papers by this authorChristophe Tournamille, Corresponding Author Christophe Tournamille christophe.tournamille@efs.sante.fr Etablissement Français du Sang Île de France Institut National de la Santé et de la Recherche Médicale (INSERM)-U955, Equipe 2: Transfusion et Maladies du Globule Rouge, Institut Mondor de Recherche Biomédicale, Créteil, France Laboratoire d'Excellence GRex, Paris, FranceAddress reprint requests to: Christophe Tournamille, Laboratoire IHM, Etablissement Français du Sang, Bâtiment UITC, Centre Félix Reyes, 2e étage, 5 rue Gustave Eiffel, 94000 Créteil, France; e-mail: christophe.tournamille@efs.sante.fr.Search for more papers by this author First published: 28 January 2017 https://doi.org/10.1111/trf.14004Citations: 2 This work was funded by the Etablissement Français du Sang, France. 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