Zoopharmacognosy is the study of the self-medication behaviors of non-human animals that use plant, animal or soil items as remedies. Recent studies have shown that some of the plants employed by animals may also be used for the same therapeutic purposes in humans. The aim of this study was to determine the antioxidant and antibacterial activity of Ceiba pentandra, Myrianthus arboreus, Ficus subspecies (ssp.) and Milicia excelsa bark crude extracts (BCE), plants consumed by western lowland gorillas (Gorilla gorilla gorilla) in Moukalaba-Doudou National Park (MDNP) and used in traditional medicine, and then to characterize their phytochemical compounds. DPPH (2,2-Diphenyl-1-Picrylhydrazyl), phosphomolybdenum complex and β-carotene bleaching methods were used to assess antioxidant activity. Antimicrobial susceptibility testing was performed using the diffusion method, while minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were assessed using the microdilution method. The highest level of total phenolics was found in Myrianthus arboreus aqueous extract [385.83 ± 3.99 mg [gallic acid equivalent (GAE)/g]. Total flavonoid (134.46 ± 3.39) mg quercetin equivalent (QE)/100 g of extract] were highest in Milicia excelsa, tannin [(272.44 ± 3.39) mg tannic acid equivalent (TAE)/100 g of extract] in Myrianthus arboreus and proanthocyanidin [(404.33 ± 3.39) mg apple procyanidins equivalent (APE)/100 g of extract] in Ceiba pentandra. Ficus ssp. (IC50 1.34 ±3.36 μg/mL; AAI 18.57 ± 0.203) ethanolic BCE and Milicia excelsa (IC50 2.07 ± 3.37 μg/mL; AAI 12.03 ± 0.711) showed the strongest antioxidant activity. Myrianthus arboreus ethanolic BCE (73.25 ± 5.29) and Milicia excelsa aqueous BCE (38.67 ± 0.27) showed the strongest percentage of total antioxidant capacity (TAC). Ceiba pentandra ethanolic BCE (152.06 ± 19.11 mg AAE/g) and Ficus ssp aqueous BCE (124.33 ± 39.05 mg AAE/g) showed strongest relative antioxidant activity (RAA). The plant BCE showed antimicrobial activity against multidrug resistant (MDR) E. coli (DECs) isolates, with MICs varying from 1.56 to 50 mg/mL and inhibition diameters ranging from 7.34 ± 0.57 to 13.67 ± 0.57mm. Several families of compounds were found, including total phenolic compounds, flavonoids, tannins and proanthocyanidins were found in the plant BCEs. The plant BCEs showed antioxidant activities with free radical scavenging and antimicrobial activities against 10 MDR E. coli (DECs) isolates, and could be a promising novel source for new drug discovery.

Abstract Conservation funding is currently limited; cost‐effective conservation solutions are essential. We suggest that the thousands of field stations worldwide can play key roles at the frontline of biodiversity conservation and have high intrinsic value. We assessed field stations’ conservation return on investment and explored the impact of COVID‐19. We surveyed leaders of field stations across tropical regions that host primate research; 157 field stations in 56 countries responded. Respondents reported improved habitat quality and reduced hunting rates at over 80% of field stations and lower operational costs per km2 than protected areas, yet half of those surveyed have less funding now than in 2019. Spatial analyses support field station presence as reducing deforestation. These “earth observatories” provide a high return on investment; we advocate for increased support of field station programs and for governments to support their vital conservation efforts by investing accordingly.

We hope to raise awareness of mental health and well-being among primatologists. With this aim in mind, we organized a workshop on mental health as part of the main program of the Winter meeting of the Primate Society of Great Britain in December 2021. The workshop was very well received. Here, we review the main issues raised in the workshop, and supplement them with our own observations, reflections, and reading. The information we gathered during the workshop reveals clear hazards to mental health and suggests that we must collectively acknowledge and better manage both the hazards themselves and our ability to cope with them if we are to avert disaster. We call on institutions and learned societies to lead in seeking solutions for the benefit of primatologists and primatology.

Cysts and trophozoites of vestibuliferid ciliates and larvae of Strongyloides were found in fecal samples from captive orangutans Pongo pygmaeus and P. abelii from Czech and Slovak zoological gardens. As comparative material, ciliates from semi-captive mandrills Mandrillus sphinx from Gabon were included in the study. Phylogenetic analysis of the detected vestibuliferid ciliates using ITS1-5.8s-rRNA-ITS2 and partial 18S ribosomal deoxyribonucleic acid (rDNA) revealed that the ciliates from orangutans are conspecific with Balantioides coli lineage A, while the ciliates from mandrills clustered with Buxtonella-like ciliates from other primates. Morphological examination of the cysts and trophozoites using light microscopy did not reveal differences robust enough to identify the genera of the ciliates. Phylogenetic analysis of detected L1 larvae of Strongyloides using partial cox1 revealed Strongyloides stercoralis clustering within the cox1 lineage A infecting dogs, humans, and other primates. The sequences of 18S rDNA support these results. As both B. coli and S. stercoralis are zoonotic parasites and the conditions in captive and semi-captive settings may facilitate transmission to humans, prophylactic measures should reflect the findings.

Stress is implicated as a major cause of failure in wildlife translocations. We monitored the stress response non-invasively during a mandrill release. Our findings suggest a pre-release enclosure allowed mandrills to recover from transfer and the mandrills adjusted well to the forest, showing the value of non-invasive physiology in translocations.

Postings on social media on Twitter (now X), BioAnthropology News (Facebook), and other venues, as well as recent publications in prominent journals, show that primatologists, ecologists, and other researchers are questioning the terms "Old World" and "New World" due to their colonial implications and history. The terms are offensive if they result in erasing Indigenous voices and history, ignoring the fact that Indigenous peoples were in the Americas long before European colonization. Language use is not without context, but alternative terminology is not always obvious and available. In this perspective, we share opinions expressed by an international group of primatologists who considered questions about the use of these terms, whether primatologists should adjust language use, and how to move forward. The diversity of opinions provides insight into how conventional terms used in primatological research and conservation may impact our effectiveness in these domains.

Olfactory communication plays an important role in the regulation of socio-sexual interactions in mammals. There is growing evidence that both human and nonhuman primates rely on odors to inform their mating decisions. Nevertheless, studies of primate chemical ecology remain scarce due to the difficulty of obtaining and analyzing samples. We analyzed 67 urine samples from five captive female Japanese macaques (Macaca fuscata) and 30 vaginal swabs from three of these females using gas chromatography-mass spectrometry and examined the relationship between odor (compounds identified, richness, intensity, and diversity) and female identity as well as cycle phase. We found a total of 36 urine compounds of which we identified 31, and 68 vaginal compounds of which we identified 37. Our results suggest that urine and vaginal odor varied more between individuals than within cycle phases. However, we found that within a female cycle, urine samples from similar phases may cluster more than samples from different phases. Our results suggest that female odor may encode information about identity (vaginal and urine odor) and reproductive status (urine odor). The question of how conspecifics use female urine and vaginal odor remains open and could be tested using bioassays. Our results and their interpretation are constrained by our limited sample size and our study design. Nonetheless, our study provides insight into the potential signaling role of female odor in sexual communication in Japanese macaques and contributes to our understanding of how odors may influence mating strategies in primates.

BACKGROUND:The occurrence of gastrointestinal parasites in the sun-tailed monkey (Allochrocebus solatus) at the CIRMF primatology center is unknown. We, therefore, assessed the presence and richness (number of different parasite taxa) of gastrointestinal parasites in a semi-free-ranging colony of A. solatus.METHODS:A total of 46 fecal samples were screened using a modified McMaster technique for fecal egg counts.RESULTS:In the 46 samples collected, seven taxa of gastrointestinal parasites, including protozoa and nematodes were identified. The most prevalent parasite was strongyles parasites (98%), followed by Trichuris spp. (72%), Strongyloides spp. (67%) and Entamoeba coli (65%). Balantioides coli (33%), Endolimax nana (25%), and Spirurid eggs (26%) were only found in a minority of the animals.CONCLUSIONS:This study contributes new host records of gastrointestinal parasites in semi-free-ranging A. solatus and highlights the need to investigate the health of this species and implement proper precautions in the management of this colony.

Female primates signal impending ovulation with a suite of sexual signals. Studies of these signals have focussed on visual, and to a lesser extent, acoustic signals, neglecting olfactory signals. We aimed to investigate the information content of female olfactory signals in captive olive baboons ( Papio anubis ) and relate these to the female fertile period. We studied eight adult females living in four groups at the CNRS Station de Primatologie, Rousset-sur-Arc, France. We used vaginal cytology to detect ovulation. We investigated the volatile component of odour signals using solid-phase microextraction and gas chromatography-mass spectrometry. We found a total of 74 volatile compounds, of which we tentatively identified 25, including several ketones, alcohols, aldehydes, terpenes, volatile fatty acids and hydrocarbons that have been identified in odour profiles of other primates. Our results show that vaginal odour intensity differs with sexual cycle stage suggesting that odour might play a role in signalling female baboon fertility. We found differences in vaginal odour between females living in all-female and in mixed sex groups but we could not distinguish the effects of group composition, female age and identity. This study of olfactory signalling improves our understanding of how female primates advertise their sexual receptivity.

Samango monkeys (Cercopithecus albogularis schwarzi) in the Soutpansberg Mountains, South Africa, experience a highly seasonal climate, with relatively cold, dry winters. They must show behavioural flexibility to survive these difficult conditions near the southern limit of the species' distribution and maintain the minimum nutritional intake they require. Through environmental monitoring and behavioural observations of a habituated group of samango monkeys, we explored how they adapted to the highly seasonal climate they experienced in the mountains. Our results indicated that the monkeys varied their foraging behaviours to account for changes in climate and daylight availability. The samangos increased their food intake in colder months, specifically leaves, likely due to an increased need for calories during winter to maintain body temperature. Samango monkeys have anatomical and physiological adaptations for digesting leaves, and these are likely important in explaining their ability to adapt to the broad range of climatic conditions they experience.

Accentuated lines in dental microstructure are hypothesized to correlate with potentially stressful life history events, but our understanding of when, how and why such accentuated lines form in relation to stressful events is limited. We examined accentuated line formation and life history events in the teeth of three naturally deceased mandrills (Mandrillus sphinx, Cercopithecidae), for whom we had detailed life history information. We determined the ages at formation of accentuated lines in histological tooth sections and used dates of birth and death to calibrate dental histology to calendar time and individual age. We found accentuated lines that matched their mother's resumption of sexual cycles in two individuals, and possibly in the third individual. The subjects also formed lines when their mothers were mate-guarded by males or wounded. Accentuated lines matched the birth of the next sibling in one of two cases. Both females formed accentuated lines when they experienced their own sexual swelling cycles, but lines did not match all sexual swelling cycles. Mate-guarding matched an accentuated line in one case, but not in another. Lines matched all three parturitions in the two females. Changes in alpha male and captures did not consistently coincide with accentuated line formation, but repeated captures were associated with lines. Using simulated data, we show that the observed number of matches between lines and events would be very unlikely under a null hypothesis of random line formation. Our results support the hypothesis that some life history events are physiologically stressful enough to cause accentuated line formation in teeth. They contribute to our understanding of how primate life histories are recorded during dental development and enhance our ability to use teeth to reconstruct life history in the absence of direct observation.

Wildlife ecotourism can offer a source of revenue which benefits local development and conservation simultaneously. However, habituation of wildlife for ecotourism can cause long-term elevation of glucocorticoid hormones, which may suppress immune function and increase an animal's vulnerability to disease. We have previously shown that western lowland gorillas (Gorilla gorilla gorilla) undergoing habituation in Dzanga-Sangha Protected Areas, Central African Republic, have higher fecal glucocorticoid metabolite (FGCM) levels than both habituated and unhabituated gorillas. Here, we tested the relationship between FGCM levels and strongylid infections in the same gorillas. If high FGCM levels suppress the immune system, we predicted that FGCM levels will be positively associated with strongylid egg counts and that gorillas undergoing habituation will have the highest strongylid egg counts, relative to both habituated and unhabituated gorillas. We collected fecal samples over 12 months in two habituated gorilla groups, one group undergoing habituation and completely unhabituated gorillas. We established FGCM levels and fecal egg counts of Necator/Oesophagostomum spp. and Mammomonogamus sp. Controlling for seasonal variation and age-sex category in strongylid infections we found no significant relationship between FGCMs and Nectator/Oesophagostomum spp. or Mammomonogamus sp. egg counts in a within group comparison in either a habituated group or a group undergoing habituation. However, across groups, egg counts of Nectator/Oesophagostomum spp. were lowest in unhabituated animals and highest in the group undergoing habituation, matching the differences in FGCM levels among these gorilla groups. Our findings partially support the hypothesis that elevated glucocorticoids reduce a host's ability to control the extent of parasitic infections, and show the importance of non-invasive monitoring of endocrine function and parasite infection in individuals exposed to human pressure including habituation process and ecotourism.

Understanding the historical context of an area enables an incoming conservationist to reflect on their role in communities and to better position themselves both politically and socially within them. Here, we explore how outside agencies and institutions, including a former colonial power, have affected and influenced local communities who share their landscape with Barbary macaques ( Macaca sylvanus ) in Bouhachem forest, north Morocco. In the context of initiating Barbary macaque conservation activities, we interviewed representatives from local governmental and nongovernmental organizations, city dwellers, and villagers about the historical, political, and social context of the study site. We found that villages around Bouhachem were politically and socially marginalized and discriminated against by the state and urban society. The existence of these divisions and the outside agencies’ simplistic view of villages as homogeneous communities negatively influenced conservation interventions, because people resisted initiatives imposed on them without prior consultation. We found that Bouhachem villagers have been, and still are, excluded from meaningful participation in the conservation of the forest and this finding encouraged us to decolonize our own practice. We engaged meaningfully with members of the surrounding communities and responded to news of erroneous stories about our activities by developing a project working in three villages that included all households. Based on our experiences, we recommend that all conservationists conduct historical and qualitative research to gain a deeper, more nuanced understanding of the communities they work in. This understanding should encourage conservationists to recognize their own social and cultural biases and to decolonize their practice. Attending to our own position may help us to avoid underestimating and alienating people who view conservation actions through a very different but equally valid lens. التخلص من التداعيات الاستعمارية في المحافظة على الرئيسيات :دراسة حالة من شمال المغرب خلاصة : إن فهم السياق التاريخي لمنطقة معينة، يمكّن المحافظ البيئي الوافد من التفكير في دوره في المجتمعات، ولانسجامٍ سياسيٍ واجتماعي أفضل داخلها. هنا نكتشف كيف أثرت الوكالات والمؤسسات الخارجية ، بما في ذلك القوة الاستعمارية السابقة، على المجتمعات المحلية التي تتعايش مع قرود المكاك البربري (Macaca sylvanus) في غابة بوهاشم شمال المغرب. في سياق بدء أنشطة الحفاظ على المكاك البربري، أجرينا مقابلات مع ممثلين من المنظمات الحكومية وغير الحكومية المحلية وسكان المدن والقرويين حول السياق التاريخي والسياسي والاجتماعي لموقع الدراسة. وجدنا أن القرى المحيطة بغابة بوهاشم كانت مهمشة و معرضة للتمييز ضدها سياسياً واجتماعياً من قبل الدولة والمجتمع الحضري. و أثر بشكل سلبي وجود هذه الانقسامات والنظرة السطحية للوكالات الخارجية للقرى على أنها مجتمعات متجانسة، على تدخلات الحفظ البيئي، لأن الناس رفضوا أي مبادرة مفروضة عليهم دون استشارة مسبقة. و وجدنا أن القرويين في بوهاشم كانوا ولا يزالون مستبعدين من المشاركة الهادفة في الحفاظ على الغابة، وهذا الاكتشاف شجعنا على التخلص من التداعيات الاستعمارية في ممارستنا. لقد تواصلنا بشكل هادف بأعضاء من المجتمعات المحيطة، واستجبنا لأخبار القصص الخاطئة حول أنشطتنا من خلال تطوير مشروع يعمل في ثلاث قرى شمل جميع الأسر. بناءً على تجاربنا، نوصي جميع دعاة الحفاظ على البيئة بإجراء بحث تاريخي ونوعي لاكتساب فهم أعمق وأكثر دقة للمجتمعات التي يعملون فيها. يجب أن يشجع هذا الفهم دعاة الحفاظ على البيئة على التعرف على التحيزات الاجتماعية والثقافية الخاصة بهم، والتخلص من التداعيات الاستعمارية في ممارساتهم.قد يساعدنا الاهتمام بموقفنا الخاص على تجنب الإبعاد و التقليل من شأن الأشخاص، الذين ينظرون إلى إجراءات الحفاظ على البيئة من منظور مختلف تمامًا لكن عادل .

OBJECTIVES We examine how dental sexual dimorphism develops in mandrills, an extremely sexually dimorphic primate. We aimed to (a) establish the chronology of dental development (odontochronology) in male and female mandrills, (b) understand interindividual and intersex variation in odontochronologies, and (c) determine how dental sexual dimorphism is achieved. MATERIALS AND METHODS We prepared histological ground sections from the permanent teeth of four female and four male mandrills from the semi-free ranging colony at the Centre International de Recherches Médicales, Franceville, Gabon. We used the microscopic growth increments in the sections to create odontochronologies. We compared ages at crown initiation, crown formation times (CFT) and crown extension rates (CER) between individuals and sexes to assess interindividual and intersex variation. RESULTS All mandrill teeth are sexually dimorphic in size. Dental sexual dimorphism in mandrills is achieved via sex differences in the duration of growth (bimaturism) and in growth rates. We also found interindividual and intersex variation in the ages at initiation and completion of crown formation. DISCUSSION Our results show that the rate of ameloblast differentiation varies between individuals and that selection for both the age at tooth initiation and CER has occurred independently in males and females to ensure that the teeth develop at appropriate times relative to the growth of the sexually dimorphic jaws. They also show that canine dimorphism is achieved through differences in both CER and CFT, unlike extant great apes or Cantius. Given at least three mechanisms for achieving canine dimorphism, we need more information to trace the evolution of this trait in primates.

In their reply to our article “A new identification of the monkeys depicted in a Bronze Age wall painting from Akrotiri, Thera” [Primates 61(3), 2019], Urbani and Youlatos (Primates https://doi.org/10.1007/s10329-020-00825-2, 2020) argue for the traditional identification of the monkeys depicted on the north and west walls of Room 6 of Building Complex Beta at Akrotiri, Thera, as vervet monkeys (Fig. 1). Their argument is based largely on previous scholarship and their analysis of monkey morphology as it appears in this Bronze Age artwork. Here, after clarifying some misconceptions and misquotations, we thoroughly contextualize the wall painting in question, emphasizing the importance of collaboration between disparate disciplines for a multifaceted and rigorous approach. The nature of the item in question is key in this reply: it is an artwork. Because the artwork in question is a cultural representation of monkeys rather than a study of live primates or preserved specimens, consideration of artistic choice, color conventions, and the agency of the artist, which are important facets of material culture, is important when answering the questions raised by Urbani and Youlatos, and should stimulate further cross-disciplinary discussions.

Publisher's copyright statement: c © Cambridge University Press 2004. This paper has been published in a revised form subsequent to editorial input by Cambridge University Press in Setchell, J.M. and Lee, P.C. (2004) 'Development and sexual selection in primates.', in Sexual selection in primates : new and comparative perspectives. Cambridge: Cambridge University Press, pp. 175-195. Information on this title: http://www.cambridge.org/gb/academic/subjects/life-sciences/biological-anthropology-andprimatology/sexual-selection-primates-new-and-comparative-perspectives?format=PB