Phosphorus (P) and sulphur (S) are essential macronutrients for crop growth, playing critical roles in physiological and biochemical processes throughout the plant life cycle, as well as in mitigating heavy metal and metalloid toxicity. Therefore, the coordinated use of P and S is crucial for optimizing crop growth and reducing the accumulation of heavy metals and metalloids in plants. While P and S signaling pathways are often studied independently, our understanding of their interactions remains limited. A series of recent studies have revealed key components regulating P-S interactions in cereal crops such as rice, maize and wheat, providing new insights into the network that integrates the signaling pathways of P and S. However, the interaction between P and S in molecular regulatory pathways, crop yield improvement, and resistance to heavy metal stress has not yet been systematically summarized or hypothesized. Here, we summarize the latest advances in P-S interactions and propose potential working mechanisms that integrate these P-S interactive regulatory pathways in cereal crops. Furthermore, we discuss the regulatory mechanisms of P-S interactions in cereal crops that still need to be uncovered in the future.

The serious threat posed by microplastics pollution to soil ecosystems and human health has attracted worldwide attention. Microplastics of different types are present in the soil environment, whereas research about the effects of different microplastics on soil ecology are limited. This study sought to determine how three common microplastics (polyethylene (PE), polystyrene (PS), and polyvinyl chloride (PVC)) affect soil physicochemical characteristics, enzyme activities, bacterial community, and their metabolic pathways at a 1 % w/w concentration. All three microplastic treatments significantly increased soil organic carbon (SOC), labile organic C fractions, and enhanced soil carbon to nitrogen ratio. PE, PS, and PVC microplastics exhibited promotion of α-glucosidase (α-GC), sucrase (SC), and alkaline phosphatase (AKP) activities. PS microplastics caused a significant increase in N-acetyl-β-D-glucosidase (NAG) and leucine aminopeptidase (LAP) activities, while PVC microplastics significantly decreased β-glucosidase (β-GC) activity. Microplastic treatments increased the bacterial community diversity while altering its composition. Proteobacteria and Chloroflexi were the dominant bacterial phyla in the soil, with microplastic treatments increasing the relative abundance of Chloroflexi and decreasing that of Proteobacteria. Functional prediction analysis indicated that microplastic treatments enriched genes involved in carbohydrate and amino acid metabolism, while reducing the abundance of genes related to signal transduction and cell motility. Correlation and pathway analyses revealed that microplastics affect bacterial community diversity and composition through direct and indirect effects (by acting on SOC or its key labile fractions), thereby influencing soil enzyme activities. In conclusion, the work emphasizes the impacts of different microplastics on soil ecosystems in terms of commonalities and dissimilarities, with the innovative finding of indirect regulation of bacterial community by SOC under microplastics contamination. This provides new perspectives for subsequent studies.

Selenium (Se) is an essential micronutrient for the human body and is closely linked to health. Rice (Oryza sativa L.), as a major staple food globally, is the primary source of Se intake for humans. To better achieve Se biofortification in rice, it is crucial to understand the molecular mechanisms behind rice’s response to different Se concentrations. This study investigates the morphological and transcriptomic responses of rice seedlings to low (1 µM, LSe) and high (10 µM, HSe) Se concentrations compared to a control (CK). Morphological analyses revealed that LSe promoted growth, enhancing shoot and root length and biomass, whereas HSe treatment inhibited these parameters, indicating Se’s dual role in rice growth. Notably, the most significant promotion of rice growth was observed at the Se concentration of 1 µM. The organic Se content and antioxidant enzyme activities (SOD, POD and CAT) in rice seedlings also reached their maximum values simultaneously. Total RNA was extracted for transcriptome sequencing, and differential gene expression analysis was conducted using DESeq2. Transcriptomic sequencing highlighted distinct responses under LSe and HSe conditions. Gene ontology (GO) enrichment analysis revealed significant involvement in processes related to oxidoreductase activity and cellular structures. KEGG pathway analysis emphasized that Se treatments notably enhanced the glutathione metabolism pathway, which is crucial for antioxidant defense. Additionally, significant changes were observed in starch and sucrose metabolism and cysteine (Cys) and methionine (Met) metabolism pathways, showing upregulation under LSe treatment and downregulation under HSe. Six key genes were validated using qRT-PCR, confirming their differential expression under varied Se treatments. The Cys, Met and starch content assays as well as qRT-PCR data demonstrated that LSe promoted the synthesis and accumulation of Cys, Met and starch, supporting enhanced growth and antioxidant capacity. Conversely, HSe inhibited the synthesis and accumulation of Cys, Met and starch in rice seedlings, and these data were also consistent with the physiological phenotype. These findings provide insights into the molecular mechanisms by which rice seedlings adapt to varying Se levels, with implications for Se biofortification and stress management strategies in crops.

Quality traits of barley (Hordeum vulgare L.) grain, such as starch and protein contents, are important factors that determine grain end-use value. In barley breeding, improved quality traits have long been a critical goal. The objective of this study was to identify underlying genetic locations for grain quality traits and mine the related candidate genes. In this study, 250 barley accessions were evaluated for key grain quality traits, including protein content (GPC), starch content (GSC) and fibre content (GFC) across a two-year period. Through genome-wide association study (GWAS) analysis, we identified 145 significant SNPs associated with grain quality traits. Additionally, two materials showed significant differences in grain quality were further selected for transcriptome analysis. Through a comparison analysis of barley grain, 4636 and 3684 differentially expressed genes (DEGs) were identified at 21 days post anthesis (DPA) and 28 DPA, respectively. These DEGs mainly participate in carbon fixation in photosynthetic organisms, starch and sucrose metabolism, and so on. By integrating GWAS and RNA-Seq data analysis approaches, we identified 17 candidate genes primarily involved in hydrolysis of starch, glycosyltransferase and post-translational modification. Altogether, 27 stable loci significantly associated with grain quality traits were detected, and 17 candidate genes related to grain quality were screened. These findings provide valuable insights for gene cloning related to grain quality and server as a reference for high-quality barley cultivars.

The Di19 (Drought-induced 19) gene family encodes Cys2/His2-type zinc finger proteins that are known to be involved in plant responses to various abiotic stresses, including drought, salinity, and temperature extremes. However, little is known about their roles in barley (Hordeum vulgare), particularly in cold stress adaptation. This study aimed to conduct a comprehensive genome-wide analysis of the barley genome to identify Di19 gene family members and examine their expression patterns under cold stress, providing theoretical support for stress-resistant barley breeding. By aligning Di19 gene sequences from Arabidopsis and rice and using BLASTp, seven HvDi19 genes were identified in barley. Bioinformatics analysis revealed that all members contain a conserved Cys2/His2-type zinc finger domain and nuclear localization signals. Phylogenetic analysis grouped the HvDi19 genes into four subfamilies, with three homologous gene pairs, and Ka/Ks analysis indicated strong purifying selection. Tissue-specific expression analysis showed significant variation in HvDi19 expression across barley organs. Under cold stress, different barley varieties exhibited distinct HvDi19 gene expression profiles: for instance, HvDi19-1 was downregulated in cold-tolerant varieties, whereas HvDi19-7 showed increased expression in a cold-tolerant mutant, suggesting their potential roles in modulating cold response. These findings reveal the evolutionary conservation and cold-responsive expression characteristics of the HvDi19 gene family, laying a foundation for future functional studies. The results also provide important molecular resources for the genetic improvement of cold tolerance in barley, contributing to the development of stress-resilient crop varieties under climate change.

Improving drought tolerance in plants is essential for increasing crop yields under water-limited conditions. In this study, we investigated the functional role of the maize gene ZmDST44, which is targeted by the miRNA ZmmiR139. Our results indicate that ZmmiR139 regulates ZmDST44 by cleaving its mRNA, as confirmed by inverse expression patterns and 5 '-RACE analysis. Overexpression of ZmDST44 in Arabidopsis, rice, and maize resulted in significant enhancements in drought tolerance. Transgenic plants exhibited reduced malondialdehyde (MDA) levels, increased proline accumulation, and upregulation of drought-responsive genes compared to wild-type plants. Transgenic Arabidopsis and rice showed improved drought resistance and higher post-drought recovery rates, and transgenic maize displayed lower sensitivity to drought stress. These findings suggest that ZmDST44 acts as a positive regulator of drought tolerance across different plant species and holds promise for developing drought-resistant crops through genetic engineering.

Partial root-zone drying irrigation (PRD) can improve water-use efficiency (WUE) without reductions in photosynthesis; however, the mechanism by which this is attained is unclear. To amend that, PRD conditions were simulated by polyethylene glycol 6000 in a root-splitting system and the effects of PRD on cotton growth were studied. Results showed that PRD decreased stomatal conductance (gs) but increased mesophyll conductance (gm). Due to the contrasting effects on gs and gm, net photosynthetic rate (AN) remained unaffected, while the enhanced gm/gs ratio facilitated a larger intrinsic WUE. Further analyses indicated that PRD-induced reduction of gs was related to decreased stomatal size and stomatal pore area in adaxial and abaxial surface which was ascribed to lower pore length and width. PRD-induced variation of gm was ascribed to the reduced liquid-phase resistance, due to increases in chloroplast area facing to intercellular airspaces and the ratio of chloroplast surface area to total mesophyll cell area exposed to intercellular airspaces, as well as to decreases in the distance between cell wall and chloroplast, and between adjacent chloroplasts. The above results demonstrate that PRD, through alterations to stomatal and mesophyll structures, decoupled gs and gm responses, which ultimately increased intrinsic WUE and maintained AN.

The traditional concentration-based health risk assessment of heavy metal (HMs) pollution in soil has often overlooked the initial loading and toxicity differences of HMs from various sources. This oversight hinders effective identification of the risky source, complicating precise risk management of soil HMs pollution. This study applied a source-oriented health risk assessment framework that integrates source profiling, exposure risk assessment, and spatial cluster analysis. Taking the Shanghai City, the largest megacity in China as a case, the findings revealed that overall environmental quality of peri-urban agricultural soil in Shanghai remains good, though 3.03 % of Cd concentrations exceeded the national reference standards. Industrial & traffic activities, primarily contributing Hg, Cd, and Pb, accounted for the highest proportion (44.3 %) of total metal concentrations and posed the greatest non-cancer risk (54.6 % for children and 53.1 % for adults). Notably, natural activities, mainly contributing Cr, ranked only third in concentration contribution (26.55 %) but induced the highest cancer risk (58.55 % for children and 57.08 % for adults). These findings suggest that sources with lower concentration contributions may still pose significant health risk. Integrating source apportionment with health risk assessment can more precisely identify the risky source and target areas for mitigating the human health hazards.

The coexistence of microplastics and benzo[a]pyrene (BaP) in the environment, and their interactions within agricultural soils in particular, have garnered widespread attention. This study focused on the early-stage interactions between microplastics and BaP, aiming to uncover their initial adsorption mechanisms. Despite the significant environmental toxicity of both pollutants, research on their mutual interactions in soil is still limited. This study conducted adsorption thermodynamics and kinetics experiments to explore the effects and mechanisms of various microplastics (polyethylene (PE), polystyrene (PS), and polyvinyl chloride (PVC)) on the adsorption of BaP. Using advanced techniques such as scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) spectroscopy, this study explored the surface characteristics of microplastics and their interactions with BaP. The results demonstrated that PVC microplastics exhibited the highest adsorption capacity for BaP, which was primarily due to π–π interactions and increased hydrophobicity. In the soil–microplastic blend systems, BaP was predominantly found on microplastics, enhancing the soil’s adsorption capacity for BaP, particularly PVC, which showed an adsorption capacity 3.69 times greater than that of soil alone. Density functional theory (DFT) simulation calculations indicated that the binding energy of BaP for PVC pretreated with soil was −59.16 kJ/mol, whereas it was −53.02 kJ/mol for untreated PVC, −39.35 kJ/mol for PE, and −48.84 kJ/mol for PS. These findings suggest that soil pretreatment enhances the adsorption stability of PVC for BaP, further elucidating the potential mechanisms behind the increased adsorption capacity in the soil–microplastic system. These findings confirm that microplastics serve as effective vectors for organic pollutants such as BaP, significantly influencing their environmental behavior in soils, and provide essential theoretical support for assessing the environmental toxicity and migration behaviors of microplastics and associated organic contaminants.

Waterlogging stress is a significant abiotic stress that has a pronounced effect on both the yield and quality of barley. Despite this, there is a lack of comprehensive understanding regarding the molecular response mechanisms involved in barley germination under waterlogging stress. In this study, we investigated five root and shoot traits related to waterlogging tolerance across 250 barley accessions. Our findings indicate that the tolerant genotype displayed lower levels of starch degradation, electrical conductivity (EC), and malondialdehyde (MDA) content compared to the sensitive genotype under waterlogging conditions. Through genome-wide association study (GWAS) analysis, we identified 77 significant Single Nucleotide Polymorphisms (SNPs) associated with waterlogging tolerance during germination. Additionally, transcriptomic analysis (RNA-Seq) revealed 1776 and 839 differentially expressed genes (DEGs) in Liu Leng Zi Da Mai (waterlogging tolerant) and Naso Nijo (waterlogging sensitive), respectively. It is noteworthy that a considerable number of these DEGs were found to be associated with transcription factors, including AP2/ERF, HSF, WRKY, MYB, and MADS. Through the integration of GWAS and RNA-Seq data analysis, a total of 32 candidate genes were pinpointed, primarily linked to plant hormones and energy metabolism regulation such as ERF, HSF, AEC and LEA. In conclusion, these findings provide novel genetic resources and insights to enhance waterlogging tolerance at germination in future barley cultivars through genomic and marker-assisted selection. This results also lay the foundation for further functional analysis of the identified candidate genes.

Recent results showed that argon may have great potential in both medicines (especially) and agriculture. However, how argon positively influences crop physiology remains elusive. Here, we observed that the stimulation of nitric oxide (NO) production upon cadmium (Cd) stress in hydroponic alfalfa root tissues was strengthened by argon-rich water and/or a NO-releasing compound. The pharmacological results further indicated that above potential source of NO stimulation achieved by argon might be attributed to NO synthase (NOS) and nitrate reductase (NR). Under hydroponic and pot conditions, the improvement of Cd tolerance elicited by argon, confirmed by the alleviation in the plant growth inhibition, oxidative damage, and Cd accumulation, was sensitive to the scavenger of NO. These results suggested a crucial role of argon-induced NO synthesis in response to Cd stress. Subsequent evidence showed that the improved iron homeostasis and increased S-nitrosylation were also dependent on argon-stimulated NO. Above results were matched with the transcriptional profiles of representative target genes involved in heavy metal detoxification, antioxidant defence, and iron homeostasis. Taken together, our results clearly indicated that argon-stimulated NO production contributes to Cd tolerance by favoring important defense strategies against heavy metal exposure.

Waterlogging is one of the major abiotic stresses in barley and greatly reduces grain yield and quality. To explore the mechanism controlling waterlogging tolerance in barley, physiological, anatomical and transcriptional analyses were performed in two contrasting barley varieties, viz. Franklin (susceptible) and TX9425 (tolerant). Compared to Franklin, TX9425 had more adventitious roots and aerenchymas and higher antioxidant enzyme activities. A total of 3064 and 5693 differentially expressed genes (DEGs) were identified in TX9425 after 24 h and 72 h of waterlogging treatment, respectively, while 2297 and 8462 DEGs were identified in Franklin. The results suggested that TX9425 was less affected by waterlogging stress after 72 h of treatment. The DEGs were enriched mainly in energy metabolism, hormone regulation, reactive oxygen species (ROS) scavenging, and cell wall-modifying enzymes. Alcohol dehydrogenase (ADH) plays an important role in response to waterlogging stress. We found that HvADH4 was significantly upregulated under waterlogging stress in TX9425. Transgenic Arabidopsis overexpressing HvADH4 displayed higher activity of antioxidant enzymes and was more tolerant to waterlogging than the wild type (WT). The current results provide valuable information that will be of great value for the exploration of new candidate genes for molecular breeding of waterlogging tolerance in barley.

Magnesium hydride (MgH2) as a solid-state hydrogen source might be potentially applied in industry and medicine. However, its biological function in plants has not yet been fully discovered. In this report, it was observed that MgH2 administration could relieve copper (Cu) toxicity in alfalfa that was confirmed by a reduction in root growth inhibition. By using old MgH2 as a negative control, it was concluded that above MgH2 function was primarily derived from the releasing of molecular hydrogen (H2), but not caused by either magnesium metabolites or pH alteration. Further results revealed that Cu-triggered nitric oxide (NO) production was intensified by MgH2. Subsequent pharmacological and biochemical experiments suggested that nitrate reductase might be mainly responsible for NO production during above processes. Cu accumulation in the root tissues was also obviously reduced in the presence of MgH2. Meanwhile, increased non-protein thiols (NPTs) content and the deposition of Cu in cell wall of seedling roots could be used to explain the mechanism underlying MgH2-alleviated Cu toxicity via NO signaling. Further, the plant redox balance was reestablished since the Cu stress-modulated antioxidant enzymes activities, reactive oxygen species (ROS) accumulation, and oxidative injury detected by in vivo histochemical and biochemical analyses, were differentially abolished by MgH2. The above responses could be blocked by the removal of endogenous NO after the addition of its scavenger. Taken together, these results clearly suggested that MgH2 control of plant tolerance against Cu toxicity might be mediated by NO signaling, which might open a new window for the application of solid-state hydrogen materials in agriculture.

Heterosis or hybrid vigor is a phenomenon that a heterozygote is superior to two parental lines in one or more traits, or even responses against stresses. However, related molecular mechanism is still not fully elucidated. In this paper, we investigated whether hydrogen sulfide (H2S), a universal signaling molecule in both mammals and plants, participates in heterosis. When upon salinity stress, two parents of Brassica napus (NJ4375 and MB1894) were more sensitive to salinity compared to the hybrid (F1, NJ4375×MB1894), evaluated by the improving effects on the reduction in shoot length, root length, fresh weight of root parts, and chlorophyll content. It was further observed that the increased endogenous H2S synthesis was more pronounced in the hybrid than those of two parental lines when stressed with salinity, suggesting the important roles of endogenous H2S. The removal of endogenous H2S by its biosynthesis inhibitor (PAG) and scavenger (HT) could differentially abolish the salinity tolerance observed in the hybrid. Subsequent experiments showed that the hybrid could maintain ion homeostasis by regulating the sodium and potassium transporters, which was confirmed by modulating NHX1, SOS2, AKT1 and HAK5 transcripts. Proline content was also significantly increased and lipid peroxidation was obviously alleviated in the hybrid plants compared to those in both NJ4375 and MB1894. Above responses in hybrid were sensitive to the inhibition of endogenous H2S synthesis or its scavenging. Overall, our results clearly provided the evidence that salt-tolerant heterosis of hybrid might be closely associated with endogenous H2S signaling.

Argon, a non-polar molecule, easily diffuses into deeper tissue and interacts with larger proteins, protein cavities, or even receptors. Some of the biological effects of argon, notably its activity as an antioxidant, have been revealed in animals. However, whether and how argon influences plant physiology remains elusive. Here, we provide the first report that argon can enable plants to cope with salinity toxicity. Considering the convenience of the application, argon gas was dissolved into water (argon-rich water (ARW)) to investigate the argon's functioning in phenotypes of alfalfa seed germination and seedling growth upon salinity stress. The biochemical evidence showed that NaCl-decreased α/β-amylase activities were abolished by the application of ARW. The qPCR experiments confirmed that ARW increased NHX1 (Na+/H+ antiporter) transcript and decreased SKOR (responsible for root-to-shoot translocation of K+) mRNA abundance, the latter of which could be used to explain the lower net K+ efflux and higher K accumulation. Subsequent results using non-invasive micro-test technology showed that the argon-intensified net Na+ efflux and its reduced Na accumulation resulted in a lower Na+/K+ ratio. NaCl-triggered redox imbalance and oxidative stress were impaired by ARW, as confirmed by histochemical and confocal analyses, and increased antioxidant defense was also detected. Combined with the pot experiments in a greenhouse, the above results clearly demonstrated that argon can enable plants to cope with salinity toxicity via reestablishing ion and redox homeostasis. To our knowledge, this is the first report to address the function of argon in plant physiology, and together these findings might open a new window for the study of argon biology in plant kingdoms.

Argon has organ-protective effects on animals. However, whether or how argon influences plant responses remains elusive. In this study, we discovered that the growth inhibition of hydroponically cultured alfalfa seedlings under 100 μM CdCl2 condition was significantly ameliorated by 100 % saturated argon-rich water (ARW). Less Cd uptake and accumulation were also observed in both root and shoot parts, which could be explained by the modified root cell walls, including the increased cell wall thickness, lignin content, and demethylation degree of covalently bound and ion-bound pectin, as well as the down-regulated expression of natural-resistance-associated-macrophage protein1 (Nramp1) encoding a heavy metal ion transporter in root tissue. The hindered Cd translocation from root to shoot achieved by ARW addition was validated by the decreased expression of heavy metal ATPase 2/4 (HMA2/4) in roots and decreased Cd content in xylem saps. The reestablished glutathione (GSH) homeostasis and redox balance, two important indicators of plant defense against Cd poisoning, were also observed. Further greenhouse experiments demonstrated that the phenotypic and physiological performances of alfalfa plants cultured in Cd-contaminated soil were significantly improved by irrigating with ARW. Above results implied that ARW confers plants tolerance against cadmium toxicity by impairing Cd uptake and accumulation and restoring GSH and redox homeostasis. These findings might open a new window for understanding argon biology in higher plants.

Waterlogging is the primary abiotic factor that destabilizes the yield and quality of barley (Hordeum vulgare L.). However, the genetic basis of waterlogging tolerance remains poorly understood. In this study, we conducted a genome-wide association study (GWAS) by involving 106,131 single-nucleotide polymorphisms (SNPs) with a waterlogging score (WLS) of 250 barley accessions in two years. Out of 72 SNPs that were found to be associated with WLS, 34 were detected in at least two environments. We further performed the transcriptome analysis in root samples from TX9425 (waterlogging tolerant) and Franklin (waterlogging sensitive), resulting in the identification of 5,693 and 8,462 differentially expressed genes (DEGs) in these genotypes, respectively. The identified DEGs included various transcription factor (TF) genes, primarily including AP2/ERF, bZIP and MYB. By combining GWAS and RNA-seq, we identified 27 candidate genes associated with waterlogging, of which three TFs (HvDnaJ, HvMADS and HvERF1) were detected in multiple treatments. Moreover, by overexpressing barley HvERF1 in Arabidopsis, the transgenic lines were detected with enhanced waterlogging tolerance. Altogether, our results provide new insights into the genetic mechanisms of waterlogging, which have implications in the molecular breeding of waterlogging-tolerant barley varieties.

MicroRNAs (miRNAs) are small, non-coding regulatory RNAs that regulate gene expression by facilitating target mRNA cleavage in plants. They are crucial for responses to diverse stresses. The novel drought-responsive miRNA ZmmiR190 was previously identified during an analysis of the maize transcriptome. In this study, we revealed that transgenic Arabidopsis thaliana overexpressing ZmmiR190 is more sensitive to drought than the wild-type control. The transcript of a nuclear-localized gene, ZmCRP04, was identified as a likely target of ZmmiR190. Moreover, ZmmiR190 and ZmCRP04 had the opposite expression profiles following drought and salt treatments. Additionally, 5 ' RACE and coexpression analyses in A. thaliana provided evidence of the in vivo targeting of the ZmCRP04 transcript by ZmmiR190. Furthermore, the overexpression of ZmCRP04 in A. thaliana and rice significantly enhanced drought tolerance, with lower malonaldehyde contents and relative electrolyte leakage in the transgenic A. thaliana and rice plants than in the wild-type control. Transgenic plants overexpressing ZmmiR190 or ZmCRP04 were hypersensitive to abscisic acid. These results suggest that the ZmCRP04 transcript is targeted by ZmmiR190 and may encode a protein that positively regulates drought stress tolerance via an abscisic acid-dependent pathway. These findings may be relevant for future molecular breeding aimed at improving crop drought tolerance.

How to use environmentally friendly technology to enhance rice field and grain quality is a challenge for the scientific community. Here, we showed that the application of molecular hydrogen in the form of hydrogen nanobubble water could increase the length, width, and thickness of brown/rough rice and white rice, as well as 1000-grain weight, compared to the irrigation with ditch water. The above results were well matched with the transcriptional profiles of representative genes related to high yield, including up-regulation of heterotrimeric G protein β-subunit gene (RGB1) for cellular proliferation, Grain size 5 (GS5) for grain width, Small grain 1 (SMG1) for grain length and width, Grain weight 8 (GW8) for grain width and weight, and down-regulation of negatively correlated gene Grain size 3 (GS3) for grain length. Meanwhile, although total starch content in white rice is not altered by HNW, the content of amylose was decreased by 31.6%, which was parallel to the changes in the transcripts of the amylose metabolism genes. In particular, cadmium accumulation in white rice was significantly reduced, reaching 52% of the control group. This phenomenon was correlated well with the differential expression of transporter genes responsible for Cd entering plants, including down-regulated Natural resistance-associated macrophage protein (Nramp5), Heavy metal transporting ATPase (HMA2 and HMA3), and Iron-regulated transporters (IRT1), and for decreasing Cd accumulation in grain, including down-regulated Low cadmium (LCD). This study clearly showed that the application of molecular hydrogen might be used as an effective approach to increase field and grain quality of rice.

Heterosis is most frequently manifested as the superior performance of a hybrid than either of the parents, especially under stress conditions. Nitric oxide (NO) is a well-known gaseous signaling molecule that acts as a functional component during plant growth, development, and defense responses. In this study, the Brassica napus L. hybrid (F1, NJ4375 × MB1942) showed significant heterosis under salt stress, during both germination and post-germination periods. These phenotypes in the hybrid were in parallel with the better performance in redox homeostasis, including alleviation of reactive oxygen species accumulation and lipid peroxidation, and ion homeostasis, evaluated as a lower Na/K ratio in the leaves than parental lines. Meanwhile, stimulation of endogenous NO was more pronounced in hybrid plants, compared with parental lines, which might be mediated by nitrate reductase. Proteomic and biochemical analyses further revealed that protein abundance related to several metabolic processes, including chlorophyll biosynthesis, proline metabolism, and tricarboxylic acid cycle metabolism pathway, was greatly suppressed by salt stress in the two parental lines than in the hybrid. The above responses in hybrid plants were intensified by a NO-releasing compound, but abolished by a NO scavenger, both of which were matched with the changes in chlorophyll and proline contents. It was deduced that the above metabolic processes might play important roles in heterosis upon salt stress. Taken together, we proposed that heterosis derived from F1 hybridization in salt stress tolerance might be mediated by NO-dependent activation of defense responses and metabolic processes.