以斑马鱼为模型,研究3种发酵乳制品的解酒保肝作用,为进一步开发其保健功能提供实验依据.使用受精后5 d斑马鱼,1％无水乙醇处理1 h构建兴奋期醉酒模型;使用受精后3 d斑马鱼,1.8％无水乙醇处理27 h构建酒精性肝损伤模型.分别使用常温酸奶、低温酸奶、冷冻酸奶冰淇凌及阳性药(美他多辛)处理两种模型斑马鱼,分析总运动距离、肝脏面积、肝脏明亮度、卵黄囊吸收延迟面积.结果表明,与对照组比较,模型组总运动距离、肝脏面积、卵黄囊吸收延迟面积明显增加(P＜0.05),肝脏明亮度明显减小(P＜0.05);与模型组比较,常温酸奶总运动距离明显减小(P＜0.05),3种发酵乳制品的肝脏面积明显减小(P＜0.001),低温酸奶组肝脏明亮度明显增高(P＜0.05),冷冻酸奶冰淇凌组卵黄囊吸收延迟面积明显减小(P＜0.05).3种发酵乳制品均具有肝保护作用,其中常温酸奶具有解酒作用.

Objective To establish the quality control standard of Changweiqing oral solution.Methods TLC were used to identify Radix Astragali,Pericarpium citri reticulatae,Radix Aucklandiae and Rhizoma Atractylodis Macrocephalae.Results TLC spots of Radix Astragali,Pericarpium citri reticulatae,Radix Aucklandiae and Rhizoma Atractylodis Macrocephalae were clear,the method was specific.Conclusion This method can be used for the quality control of Changueiqing Oral Solution.