AIM:The aim was to build an exosome-related gene (ERG) risk model for thyroid cancer (TC) patients. METHODS:Note that, 510 TC samples from The Cancer Genome Atlas database and 121 ERGs from the ExoBCD database were obtained. Differential gene expression analysis was performed to get ERGs in TC (TERGs). Functional enrichment analyses including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were conducted on the TERGs. Then we constructed a model based on LASSO Cox regression analysis. Kaplan-Meier survival analysis was applied and a Nomogram model was also built. The immune landscape was evaluated by CIBERSORT. RESULTS:Thirty-eight TERGs were identified and their functions were enriched on 591 GO terms and 30 KEGG pathways. We built a Risk Score model based on FGFR3, ADRA1B, and POSTN. Risk Scores were significantly higher in T4 than in other stages, meanwhile, it didn't significantly differ in genders and TNM N or M classifications. The nomogram model could reliably predict the overall survival of TC patients. The mutation rate of BRAF and expression of cytotoxic T-lymphocyte-associated protein 4 were significantly higher in the high-risk group than in the low-risk group. The risk score was significantly correlated to the immune landscape. CONCLUSION:We built a Risk Score model using FGFR3, ADRA1B, and POSTN which could reliably predict the prognosis of TC patients.

Abstract Background: Acute abdominal pain is often caused by intestinal obstruction, with high morbidity, and mortality, so that the early diagnosis is particularly important. Currently, both spiral CT and ultrasound are common imaging diagnostic methods. However, the accuracy and practicality of the diagnosis are controversial. Therefore, the purpose of this study is to systematically evaluate the accuracy and practicality of spiral CT and ultrasound in the diagnosis of intestinal obstruction. Methods: Retrieval of English database (PubMed, Embase, Web of Science, the Cochrane Library) and Chinese database (CNKI, WAN FANG, VIP, CBMDISC) by computers. From the establishment of the database to October 2020, a diagnostic experimental study on the diagnosis of intestinal obstruction by ultrasound and spiral CT was conducted. Two researchers independently conducted data extraction and quality evaluation of literature on the included studies, and Meta Disc1.4 and RevMan5.3 were used for meta-analysis on the included literature. Results: Sensitivity, specificity, po-sitive Likelihood ratio, NE-Gative likelihood ratio, diagnostic odds ratio and other indicators were used to determine the diagnostic efficacy of ultrasound and helical CT. Conclusion: This study is aimed at providing an evidence-based basis for clinicians to choose an appropriate or optimal diagnostic method by comparison of the accuracy and practicality between spiral CT and ultrasound in the diagnosis of intestinal obstruction. Ethics and dissemination: The private information from individuals will not be published. This systematic review also will not involve endangering participant rights. Ethical approval is not required. The results may be published in a peer-reviewed journal or disseminated in relevant conferences. OSF Registration number: DOI 10.17605/ OSF.IO / Q5RNS.

目的 探讨二维超声和超微血流成像(SMI)在宫颈癌术前分期及术后盆底功能评估中的价值.方法 选取在我院行手术治疗的宫颈癌患者62例,均行二维超声、SMI及病理组织活检,以病理结果为金标准,比较二维超声和SMI对宫颈癌术前分期的准确性,以及二维超声评估术后盆底功能的价值.结果 二维超声和SMI对宫颈癌术前分期的诊断准确率分别为70.97％和80.65％,两者联合应用诊断准确率为90.32％.术后二维超声检查示26例(41.93％)患者发生前盆腔脱垂,10例(16.13％)发生压力性尿失禁,9例(14.52％)发生后盆腔脱垂,33例(53.23％)患者术后发生盆底功能障碍.宫颈癌术后患者最大Valsalva下膀胱颈移动度、尿道旋转角度、静息下膀胱颈尿道后角、最大Valsalva下膀胱尿道后角均明显高于术前,差异均有统计学意义(均P<0.05).结论 二维超声联合SMI对宫颈癌术前分期的诊断准确性较高,有助于临床制定治疗方案;二维超声对术后盆底功能的评估具有较好价值.