Fibrous plants with higher biomass, particularly industrial hemp, have ability to withstand and accumulate significant quantities of heavy metals from contaminated environments. The present study aimed to evaluate the dynamics of different levels (ratios) of macronutrients nitrogen, phosphorus and potassium (NPK) viz., NPK1--NPK (1:1:1); NPK2--NPK (2:1:1); NPK3--NPK (3:1:2); NPK4--NPK (4:1:2) on hemp growth and Cu contents under various levels of Cu stress (100, 400 and 800 mg kg- 1 on dry soil basis using CuSO4 & sdot;5H2O). Results revealed that by increasing the Cu stress, growth and biomass decreased linearly and lipid per oxidation and enzymatic antioxidants increased. Balanced application of NPK improved the biomass and decreased the membrane damage by the modulation of malonaldehyde contents. Maximum concentration of Cu in roots (377.47 +/- 4.90 mg kg-1), shoots (137.45 +/- 5.60 mg kg-1) and (150.07 +/- 3.57 mg kg-1) was recorded at Cu3NPK2 treatment as compared to control. Maximum translocation factor (TF) and bioaccumulation coefficients (BAC) in the shoots and leaves of hemp plant were noticed where Cu stress was applied at the rate of 100 mg kg- 1. However, BAC and TF were below 1. The NPK2 treatment enhanced biomass and increase Cu content both in leaves and stems, rather than the roots. Our study suggests that balanced application of NPK is a practicable approach to alleviate Cu stress and improve biomass production of industrial hemp plant. These findings indicate that optimum nutrient supply, under Cu stress, can maximize the growth potential and overall health of industrial hemp, making it a viable option for phytoremediation and sustainable agriculture on contaminated soils.

This study presents a comprehensive reservoir characterization of the channel sandstones of the Pliocene El Wastani Formation from the deepwater Scarab field, in Egypt. Routine core analysis, wireline logs, petrographic thin sections, X-ray diffraction, and drilling data were integrated to characterize the petrographical, petrophysical and geomechanical properties of the studied gas reservoirs to infer the implications for reservoir development. The petrographic analysis indicates fine to medium-grained glauconitic subfeldspathic wacke with dominantly primary intergranular porosity and minor secondary porosity contributed by feldspar dissolution. The reservoir facies exhibit pore-filling detrital clay (dominantly illite, smectite mixed-layer clay) along with minor carbonate and silica cementation. These reservoirs are megaporous and consist of an isotropic pore system, with 20-35 % porosity, 2400-3400 mD horizontal permeability, and 1300-2900 mD vertical permeability. Wireline log-based petrophysical assessments indicate excellent reservoir qualities with low shale volume (9-23%) and high hydrocarbon saturation of about 65-85%. Based on the geomechanical analysis, rock-mechanical properties, pore pressure, vertical stress, and minimum horizontal stress magnitudes were interpreted. The production and depletion-related risks were analyzed by utilizing geomechanical modeling which provided a quantitative assessment of drawdown and depletion limit to ensure sand-free hydrocarbon production without the risk of shear slippage on pre-existing weak planes. Considering the average unconfined compressive strength of 14 MPa, the Pliocene reservoir will require a 9.2 MPa depletion or 16.9 MPa drawdown to reach the onset of sanding, while the reservoir can be depleted to a pore pressure level of 4 MPa before it induces shear slippage on the optimally oriented fractures or fault planes. Inferences are drawn on reservoir quality, and reservoir development strategies are discussed accordingly.

The lithofacies, microfacies, and depositional environments of the Dhruma Formation were studied at the type locality at Khashm adh Dhibi to better understand the sedimentology and depositional history on the Arabian Platform. Twelve lithofacies were identified that can be grouped into four lithofacies associations corresponding to depositional paleoenvironments ranging from peritidal to open marine. The vertical distribution of the lithofacies and their corresponding depositional settings allow for the division of the Dhruma Formation into six 3rd-order sequences (DS1–DS6), each bounded by sharp vertical facies changes, and/or hiatuses. Abrupt negative shifts in both δ13Ccarb and δ18Ocarb values coincide closely with the placement of sequence boundaries on the Arabian Platform. This suggests that the carbonates in these zones underwent meteoric diagenesis during episodes of subaerial exposure. Comparison of the sequence stratigraphy interpreted from the vertical distribution of facies with published eustatic sea-level curves improves our understanding of the eustatic sea-level variation and/or local tectonics in forming both local hiatuses and regional unconformities.

To reduce dependency on synthetic fertilizers in response to the escalating costs of fertilizers and environmental limitations, it is imperative to enhance crop productivity and soil fertility sustainably. This research was carried out at the Agricultural Research Farm of Abdul Wali Khan University in Mardan, Pakistan, with the objective of investigating the effects of biochar on the qualitative attributes of mung beans. The randomize complete block design (RCBD) was used for the experiment having four replication. The treatments comprised of four levels of biochar i.e. 0, 10, 20 and 30 t ha−1. Our results revealed that increasing biochar content caused an increase in yield components as well as attribute composition. The nodule density (17.8), pods plant−1 (27.3), grains pods−1 (11.4) and biological yield (6497 kg ha−1) produced best results under the application of 30 t ha−1 of biochar. Moreover, grain yield (1550 kg ha−1), grain nitrogen content (25.2 g kg−1) and straw nitrogen content (15.3 g kg−1) also resulted best under 30 t ha−1 biochar. While, 1000 grain weight (64 g) was recorded highest weight under 20 t ha−1. The quality attributes showed that the oil content (41.1

The proper maintenance of soil physiochemical properties in grassland ecosystems through independent management practices like grazing and mowing have strongly influenced the soil quality and grassland yield. Less known is, to declare the best -fit management strategy for the grassland ecosystem. The present study was performed to search for the best -fit management system for the grassland ecosystem in northern China by evaluating the consequences of grazing and mowing on soil physiochemical properties like soil moisture, bulk density, electrical conductivity, pH, and total concentrations of C, N, and P. We found that compared to mowing, grazing significantly increased soil moisture, bulk density, and N concentration by 12%, 7%, and 14%, respectively. However, no significant effect of grazing was observed on soil C and P concentrations and C: N, C: P, and N: P ratios. However, grazing was found to strongly affect soil physiochemical properties; in contrast, mowing did not alter the soil C, N, and P concentrations and their stoichiometric ratios. Further, physical properties were altered more significantly than the soil chemical properties. This study suggests that, compared to mowing, cattle grazing has more positive impacts on soil physicochemical properties which will be the best -fit management strategy for the grassland ecosystem in northeastern China. A comprehensive investigation of long-term grazing and mowing on soil physicochemical properties may enable us to predict further better understandings.

This study presents the petrographical and petrophysical characteristics of the Cambro-Ordovician clastic reservoirs from the Risha field, northeastern Jordan. Routine core analysis, wireline logs, petrographic thin sections, scanning electron microscopy, and X-ray diffraction were integrated to characterize the gas reservoirs of the Risha, Dubeidib, and Umm Sahm formations (the equivalent of Sarah, Qasim, and Upper Saq formations of northern Saudi Arabia). These reservoirs are variably micro- and mesoporous, with permeability < 1 mD and dominantly < 6

For thousands of years, plants have been utilized for medicinal purposes. For its naturally existing antibacterial properties, Nigella sativa is one of the most researched herbs. A study was conducted during rabi 2020-21 at The University of Haripur in order to evaluate the potential of ascorbic acid as plant growth enhancer. Two concentrations of ascorbic acid i-e 350 μm and 400 μm were sprayed along with control and water only spray on Nigella sativa crop. The study was arranged in RCBD two factor factorial arrangement. Factor A: ascorbic acid concentrations along with control and water spray, factor B: Growth stages (Stage1 = 40 days after sowing, Stage 2 = 80 DAS, Stage 3 = 120 DAS, Stage 4 = 40 + 80 DAS, Stage 5 = 40 + 120 DAS, Stage 6 = 80 + 120 DAS, Stage 7 = 40 + 80 + 120 DAS). Crop was sown in first week of November. Results reviled that chlorophyll b content, fixed oil content, 1000 seed weight, grain yield, Photosynthetic rate (μ mole m−2s−1), Transpiration rate (mmole m−2s−1), photosynthetic water use efficiency, Internal CO2 concentration (Ci) of leaf tissue and Stomatal conductance (mmole m−2s−1) were significantly affected by ascorbic acid concentrations and stage of application. Crop growth rate increased by 19.88% and 17.29%, chlorophyll b by 12.3% and 11.2%, fixed oil by 11.7% and 9%, grain yield by 10.29% and 9.8%, harvest index by 4% and 5.7% photosynthetic rate by 33%, 20% and stomatal conductance by 24.24% and 24.25 with application of ascorbic acid @ 350 μm, over control and water spray respectively. On the basis of these results it is concluded that application of ascorbic acid at the rate of 350 μm, followed by ascorbic acid at the rate of 400 μm significantly improves black cumin (Nigella sativa) yield and production. Hence it is recommended to apply ascorbic acid at the rate of 350 μm at 40 + 80+120 days after sowing of Nigella sativa crop for obtaining maximum results.

Geochemical analysis was performed on the Cretaceous sequence of the Azhar‐A‐2 well in the West Beni Suef Basin (WBSB), Western Desert, Egypt, utilizing data on total organic carbon (TOC), kerogen composition, vitrinite reflectance (Ro%) and Rock‐Eval pyrolysis. In addition, a 1D basin model was built to investigate the burial and temperature history of the study area. The most important Cretaceous source rocks are predominantly reported within the Albian Kharita Formation and Late Cenomanian‐Santonian Abu Roash (AR) Formation. Based on visual kerogen tests and Rock‐Eval pyrolysis, AR Formation (A, E, F, and G) are mixed oil‐ and gas‐prone source rocks with kerogens ranging from type II to type III, where A/R ‘A and F’ Members show dominant oil‐prone kerogen with the highest generative potential, while A/R ‘E and G’ Members are more gas‐prone kerogen. Most samples of the lower Kh Formation were interpreted as gas‐prone kerogen type III with low generative potential. On the other hand, the high amount of liptinite in the visual kerogen macerals is a strong indicator that the lower Petroleum Formation is an oil‐prone rather than gas‐prone source rock. The thermal maturity of the studied members increases consistently with depth, ranging from immature at the top of the AR Formation to the main/peak oil window at the base of the Lower Kharita Formation, which served as an active source rock for the hydrocarbons generated in the WBSB. The high value of the heat flow in the Beni Suef Basin (57 and 60 mW/m2) is a good indicator for the shallowing of the active source rock depth limit depth. From the study of the basin modeling, the main mature zone reached between 9811 and 11,090 ft in the middle of the Late Cretaceous (84.3–82.5 Ma) is through the L. Kharita Formation with three phases of hydrocarbon generation according to transformation ratio, where the second phase is the main stage in which TR is 5%–50%, showing the beginning of oil expulsion (Ro: 0.73%–0.78%, possibly 0.81%).

Soil bioagents, such as beneficial bacterial and fungal isolates, have been extensively investigated due to positive impact on plant growth. Present trail was planned to check the effects of bacterial and fungal bioagents on the growth, physiology, quality characteristics of potato plants, as well as the enzymatic attributes of the soil. Experimental treatments were comprised different species of bacterial and fungal bioagents i.e., Glutamicibacter protophormiae, Streptomyces spectabilis and Penicillium chrysogenum mixed in the soil and treatment without these agents was considered as control. Results proved that the incorporation of bioagents has beneficial effects on the biomass (fresh and dry), photosynthetic activity, and quality attributes of potato. Bacterial isolates exhibited a modest enhancement in the growth and quality traits of the potato, in contrast to fungal isolates. The addition of Penicillium chrysogenum resulted in a reduction of lipid peroxidation in upper parts (leaves) and enhanced the activity of enzymatic antioxidants, surpassing the effects observed with bacterial bioagents and the control group. A negative relationship was identified between enzymatic activity in leaves and photosynthetic traits. Moreover, addition of fungal isolate improved the carotenoid contents (46.15

This study presents the first-ever preliminary assessment of the potential reservoir intervals from the Cambro-Ordovician Shifah Formation of the Shushan Basin, Western Desert. Seismic data, thin sections, XRD, XRF, and wireline logs were integrated to characterize the potential intervals. The study area is characterized by E-W and ENE-WSW trending steeply dipping normal faults creating a series of horsts, grabens, and half-graben structures. Based on the wireline logs-based petrophysical assessment, three potential reservoir intervals are identified with the Shifah Formation below 15000 ft depth. All the promising intervals show little shale volume (10–20 %) and 70–80 % hydrocarbon saturation, but the porosity is dominantly below 10%. The two potential sandstone intervals are composed of quartz arenites affected by silica cementation and mechanical compaction. These glauconite-bearing sandstones exhibit low-angle cross-lamination and planar lamination, likely deposited in a fluvial to shallow marine depositional environment. Both the tight sandstone intervals exhibit E-W, and NW-SE striking, closed, and partially open fractures which can contribute to the reservoir flow capacity. The third potential reservoir interval is an igneous intrusive body, composed of alkali syenite and exhibits secondary intraparticle porosity due to partial feldspar dissolution and minor fractures. The preliminary assessment presented in this work shed critical insights into the reservoir potential of the Shifah Formation.

Application of pear twig derived biochar and nitrogen fertilizer is strategic for addressing the challenges posed by copper pollution in soils. Their combined use aims to improve plant health and promote sustainable agricultural practices, which leads to better potato growth and quality. Therefore, this study was carried out to investigate the effects of different levels of pear twig biochar (B0:0, B1:3, B2:5, B3:7% w/w) combined with nitrogen fertilizer (N0:0, N1:150, N2:200, N3:250, N4:300 mg kg- 1) on morpho-physiological growth and copper uptake of potato cultivated in Cu polluted soil. Results showed that combined approach of pear twig biochar and nitrogen significantly influenced morpho-physiology, antioxidant enzyme activity, mineral content and tuber quality of potato. B2N3 significantly increased the plant height and chlorophyll in plants as compared to B0N0 (control). Malondialdehyde and proline contents were highest in control; however, maximum reductions in MDA and proline contents were recorded at B2N4 (70.32% and 92.12% at budding stage, respectively) and at B2N3 (82.44% and 91.93% at flowering stage, respectively). Likewise, B2N3 showed maximum reduction in activities of peroxidase (7343.47 and 11077.27 U g-1), catalase (1184.98 and 165.64 U g-1) and superoxide dismutase (14.84 and 19.94 U g-1) at budding and flowering stages, respectively. However, lowest contents of soil available Cu (2.03 +/- 0.5 mu g g-1) and tuber flesh Cu (4.44 +/- 0.3 mu g g-1) were recorded at B2N3 as compared to control. Interestingly, 7% biochar at all levels of nitrogen exhibited a significant decrease in soil available Cu and tuber flesh Cu. Tuber quality traits were also significantly improved at B2N3 as compared to control. However, future research and field trials can help refine the best practices for integrating these elements in different agricultural systems.

After several years of gas production, a black solid hydrocarbon material began to be produced along with the gas stream from the Risha gas field, in the northeastern part of Jordan. The main reason for the formation of this material is still unknown until this time, so knowing the source of this material is this study's main goal. Organic geochemical studies for black solid material and effective source rocks (Lower Silurian shales) are used to determine the physical and organic components of the produced black solid material (heavy oil) and attempt to identify its source. The black solid material demonstrates high organic carbon (TOC = 12% by leco instrument) and a high calorific value, like those of oils, asphalts, and shale oils with moderate sulfur content. The gross composition of the black solid material sample is dominated by saturates and asphaltene but contains very low aromatic hydrocarbons reflecting the naphthenic oil type. Meanwhile, the Silurian extracts range between paraffinic and paraffinic-naphthenic organic matters. The biomarker indicators related depositional environment and carbon isotopic composition for the black solid material sample and Silurian extracts, show that they were relatively deposited in a reducing marine environment. The black material reveals lower maturity level than the Silurian extracts. Oil/source rock correlation study for the studied samples suggests strong affinity between the black material and Lower Silurian source rock in their source characteristics, suggesting that the black hydrocarbon material was expelled from the Silurian source rocks at low maturity stage as normal oil. This normal oil is subjected to secondary alteration process (evaporative fractionation) and was stored in the reservoir. It contaminated with some inorganic material that was introduced into the well as a component of a drilling mud additive or drill string lubricant or, potentially, in part, a corrosion product of wellbore tubulars, since significant iron was present in the sample.

Agave species are typical crassulacean acid metabolism (CAM) plants commonly cultivated to produce beverages, fibers, and medicines. To date, few studies have examined hemicellulose biosynthesis in Agave H11648, which is the primary cultivar used for fiber production. We conducted PacBio sequencing to obtain full-length transcriptome of five agave tissues: leaves, shoots, roots, flowers, and fruits. A total of 41,807 genes were generated, with a mean length of 2394 bp and an annotation rate of 97.12 % using public databases. We identified 42 glycosyltransferase genes related to hemicellulose biosynthesis, including mixed-linkage glucan (1), glucomannan (5), xyloglucan (16), and xylan (20). Their expression patterns were examined during leaf development and fungal infection, together with hemicellulose content. The results revealed four candidate glycosyltransferase genes involved in xyloglucan and xylan biosynthesis, including glucan synthase (CSLC), xylosyl transferase (XXT), xylan glucuronyltransferase (GUX), and xylan α-1,3-arabinosyltransferase (XAT). These genes can be potential targets for manipulating xyloglucan and xylan traits in agaves, and can also be used as candidate enzymatic tools for enzyme engineering. We have provided the first full-length transcriptome of agave, which will be a useful resource for gene identification and characterization in agave species. We also elucidated the hemicellulose biosynthesis machinery, which will benefit future studies on hemicellulose traits in agave.

As a highly active signaling molecule, nitric oxide (NO) is a key factor in regulating plant growth and development. Nitric oxide can promote the formation of symbionts between plant roots, rhizobia and AMF, which improves plant access to nitrogen and phosphorus nutrients in the soil. As a signaling molecule, NO regulates plant resistance to biological and abiotic stresses through the following mechanisms: 1) NO interacts with ROS to regulate reactive oxygen levels and mitigate the damage of oxidative stress response on plants; 2) NO regulates plant immunity and stress resistance through post-translational modification of proteins; 3) NO interacts with various plant hormones and is involved in the regulation process of plant growth and development by hormones. In addition, NO can promote the expression of genes related to the formation and development of the symbionts, inhibit the expression of immune genes, and maintain the REDOX level and energy state of the symbionts through the cycle of NO and phytoglobin, thereby enhancing the plant microbial symbiosis. Previous studies on NO have mainly focused on the first three aspects, and there have been few studies on the mechanism of NO in plant-microbe interaction, therefore the involvement of NO in the mechanism of plant-microbe interaction should be strengthened. It is of great theoretical and practical importance to uncover the mechanism of NO enhancement of plant stress resistance and regulation of root development and to study the mechanism of NO regulation of plant-microbe interactions, responses to abiotic stresses, stomatal regulation, and a range of developmental processes. Combined with recent advances in plant NO biology, this review will highlight some key aspects that need further attention.

Marine cyanobacterial bioactives, due to their diverse chemistry and promising pharmacological properties, hold significant potential as therapeutic agents. Here, we comprehensively review the scientific state-of-the-art relating to marine cyanobacterial bioactives, including bioprospecting, preclinical and clinical studies, mechanisms of action, safety and toxicity considerations, sustainability, and conservation. We discuss challenges in and opportunities for developing marine cyanobacterial bioactives as therapeutics; we underscore their potential in treating life-threatening health ailments, such as cancer and neurodegenerative, inflammatory and viral diseases. We further articulate the significant potential of marine cyanobacterial bioactives for improving human health, and identify future research directions underpinning their development as therapeutics.

Salt stress has emerged as a growing global concern, exerting a significant impact on agricultural productivity. The challenges of salt stress on potatoes are crucial for ensuring food security and sustainable agriculture. To address this issue a pot trial was executed to evaluate the impacts of NaCl in the soil on the growth, photosynthetic pigments, and quality attributes of potato, plants were grown in soil spiked with various concentrations of NaCl (0, 1, 3, 5, 7 g kg-1 of soil). Results revealed that salt stress have negative impacts on the growth, biomass, photosynthesis and quality attributes of potato. Lower level of salt stress 1 g kg-1 of soil improved the fresh and dry biomass of leaves (78.70 and 47.74%) and tubers (86.04 and 88.92%) as compared to control, respectively. Higher levels of salt stress (7 g kg-1) increased lipid peroxidation in leaves and improved the enzymatic antioxidants. It was observed that enzyme activities i.e., SOD (134.97%), POD (101.02%), and CAT (28.87%) increased in leaves and are inversely related to the NaCl concentration. The combination of reduction in chlorophyll contents and soluble sugars resulted in lower levels of quality attributes i.e., amylose (68.90%) and amylopectin (16.70%) of potato. Linear relationship in growth, biomass and physiological attributes showed the strong association with increased salt stress. Furthermore, the PCA-heatmap synergy offers identifying clusters of co-regulated attributes, which pinpoint the physiological responses that exhibit the strongest correlation with increasing salt stress levels. Findings indicate that potato can be grown successfully with (1 g kg-1 of NaCl in soil) without negative impacts on plant quality. Furthermore, this study contributes valuable insights into the complexities of salt stress on potato plants and provides a foundation for developing strategies to enhance their resilience in salt-affected environments.

Aims Tillage and mulching are two agricultural practices that play a crucial role in improving crop growth. Tillage involves mechanical manipulation of the soil, which helps improve soil aeration, drainage, and root penetration. By loosening the compacted soil, tillage allows plant roots access to nutrients and ensures adequate drainage, which is essential for both healthy root development and optimal crop growth. However, their combined influence on soil enzymatic activities along with maize growth still needs further investigation. Methods That’s why current study was conducted with two factors (different tillage techniques and mulching levels) and four replications. No tillage, conventional tillage and deep tillage practices was done with and without wheat straw mulching (8 kg/ha). Results Results showed that deep tillage with mulching caused significant improvement in days to emergence (9.98%), emergence/m 2 (12.18%), plant height (8.97%), leaf area/plant (17.33%), grains/ear (6.91%), 1000 grains weight (4.72%), grains yield (1.88%) and biological yield (13.29%) over no tillage and no mulching. It also significantly improved total soluble sugar, total protein, free proline, total carbohydrates, urease activity, alkaline phosphatase activity, invertase and catalase activity (D) compared to no tillage and no mulching. Conclusion In conclusion, deep tillage with mulching is a better technology than conventional tillage and no tillage with mulching to improve maize growth and quality attributes. It can also regulate the soil enzymes and health in positive manner. More investigations are suggested at field level using different cereal crops for declaration of deep tillage with straw mulching as best treatment for enhancement of their production.

Vigna radiata (L.) Wilczek belongs to the Fabaceae family and it's the third-largest major pulse crop in terms of area (101,566 acres) and yield (36,954 Mt). Over the past few decades, the climate of the planet has changed significantly, affecting all stages of physiological development and plant growth. In the current study, mathematical hydro time model (HT), thermal time model (TT) and hydrothermal time models were used to explore the physiological responses of seed germination to osmotic potential, temperature and their interaction (Osmotic Potential pound Temperature). A lab experiment was performed in an incubator using the randomized complete block design (RCBD) at different osmotic potentials ( s) and temperatures (Ts). Differ-ent constant temperatures (20, 25, 30, 35, 40 and 45 degrees C) with five each s (0,-0.3,-0.6, 0.9 and-1.2 MPa) were used in the experiment. The results showed that rising the temperature between 25 and 35 degrees C enhanced the germination rate (GR). Overall, GR seems to be very sensitive to changes than T. The maxi-mum value of GP (germination percentage) is recorded in control (0 MPa) at 30 degrees C and the lowest was at-1.2 MPa in 45 degrees C. At each Temperature, the GP decreased as decreased. The uH values were recorded max-imum at 30 degrees C at 0 MPa and minimum in 45 degrees C at-1.2 MPa. T50% recorded significantly high in 30 degrees C at 0 MPa and the lowest value showed in 45 degrees C at (-1.2 MPa). Germination characteristics increased at optimal temper-ature and reduced as water potential dropped at each temperature. Literature that is currently accessible indicates that no particular seed germination patterns studies utilizing the hydrothermal time model have been published for Vigna radiata var. Ramzan.(c) 2023 SAAB. Published by Elsevier B.V. All rights reserved.

To address food production challenges and combat heavy metal toxicity, an economically viable and sustain-able approach is essential. Cadmium (Cd) contamination poses risks to crops and human health. Leveraging the structural similarity between zinc (Zn) and cadmium, we explored foliar Zn spray's potential to alleviate Cd toxicity and enhance wheat yield. Our field study near Peshawar, Pakistan, during the 2021-2022 winter, employed a split-plot design to investigate Zn's impact on wheat biofortification. Across three wheat varie-ties (JANBAZ-10, Pirsabak (PS)-2013, PS-2015), four foliar Zn levels (0 %, 0.5 %, 1.0 %, 1.5 % solution) were tested at various growth stages. Pirsabak-2015 exhibited optimal results, with a 1 % foliar Zn treatment pro-ducing the highest grain yield (3409 kg ha -1), biological yield (9022 kg ha -1), grains per spike (56), and 1000-grain weight (40.6 g). Application of 1 % biochar also significantly increased grain yield. Zn concentra-tion peaked in grains and leaves with 1.5 % Zn solution, followed by 1.0 % Zn in the PS-2015 cultivar. More-over, 1 % Zn application enhanced photosynthetic parameters and significantly reduced cadmium concentrations in leaves and grains. Notably, 1.0 % Zn led to improved stress tolerance as indicated by decreased electrolyte leakage and increased superoxide dismutase and peroxidase activities. Applying 1.0 % Zn solution as a foliar spray during different growth stages demonstrates the potential to enhance wheat yield, increase grain Zn content, and mitigate Cd toxicity in Cd-contaminated soil. Further research across diverse cereal crops and climates will help establish 1.0 % Zn foliar spray as an effective Cd stress mitigation strategy.(c) 2023 SAAB. Published by Elsevier B.V. All rights reserved.