A radioimmunoassay for plasma corticosterone has been developed. Antiserum against corticosterone was produced in rabbits immunized with corticosterone -21- hemisuccinate conjugated to bovine serum albumin. The assay was practical and reliable. The coefficient of variation between assays was ± 23% and among assays was ± 8%. Plasma corticosterone of mice is measured readily by assaying directly aliquots of a methylene chloride extract of 40 μl of plasma. The mean plasma corticosterone concentration of mice was similar to that obtained by other methods.
Four triterpenes have been isolated from the fruits of Cabralea polytricha A.Juss. (Meliaceae). Two of them are new C30 compounds whose structures have been established as the C-3, C-24 epimer of ocotillol-II and the C-24 epimer of ocotillone-II: 20S, 24S-epoxy-dammarane-3α,25-diol (IIa) and the corresponding 3-keto compound (IVa).