A micro-patterned cell adhesive surface was prepared for future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro-patterns were prepared by a photolithography process. Afterwards, recombinant filamentous phages that displayed a short binding motif with a cell adhesive peptide (-RGD-) on p8 proteins were immobilized on PDMS microgrooves through simple contact printing to study the cellular response of rat H9c2 cardiomyocyte. While the cell density decreased on PDMS micro-patterns, we observed enhanced cell proliferation and cell to surface interaction on the RGD-phage coated PDMS microgrooves. The RGD-phage coating also supported a better alignment of cell spreading rather than isotropic cell growths as we observed on non-pattered PDMS surface.

Herein, a micro-patterned cell adhesive surface is prepared for the future design of medical devices. One-dimensional polydimethylsiloxane (PDMS) micro patterns were prepared by a photolithography process. We investigated the effect of microscale topographical patterned surfaces on decreasing the collective cell migration rate. PDMS substrates were prepared through soft lithography using Si molds fabricated by photolithography. Afterwards, we observed the collective cell migration of human lens epithelial cells (B-3) on various groove/ridge patterns and evaluated the migration rate to determine the pattern most effective in slowing down the cell sheet spreading speed. Microgroove patterns were variable, with widths of 3, 5, and 10 µm. After the seeding, time-lapse images were taken under controlled cell culturing conditions. Cell sheet borders were drawn in order to assess collective migration rate. Our experiments revealed that the topographical patterned surfaces could be applied to intraocular lenses to prevent or slow the development of posterior capsular opacification (PCO) by delaying the growth and spread of human lens epithelial cells.

Event Abstract Back to Event Bladder cancer-on-a-chip for analysis of tumor transition mechanism Eojin Lee1, Chunga Kwon1, Hyungseop Han1, Jimin Park1, Yu-Chan Kim1, Myoung-Ryul Ok1, Hyun-Kwang Seok1 and Hojeong Jeon1 1 Korea Institute of Science and Technology, Center for Biomaterials, Korea Metastatic bladder cancer shows 6% survival rate within 5 years of diagnosis and usually causes death after 2 years. Purpose of this study is to evaluate the effect of extracellular matrix proteolytic enzyme MMP-9 and cell adhesion molecule CD44, which are involved in invasion and metastasis of cancer, using muscle-invasive bladder cancer cell line (RT4) and superficial human bladder carcinoma cell line (5637) to more effectively prevent migration and metastasis of bladder cancer. 3-D microfluidic device was created using PDMS and matrigel, mixed ECM similar to bladder tissue was injected into the cell culture channel. After gelation, RT4 and 5637 bladder cancer cell lines were cultured and stained to observe metastasis over time using fluorescence microscope and a confocal microscope. Observation after 2 weeks showed increased expression of CD44 and RT4 muscle-invasive bladder cancer cells compared to 5637 superficial carcinoma cell line. Zymography analysis using sup collected from conditioned medium during RT4 and 5637 cell culture process in microfluidic channel showed the significant increase of MMP-9 gelatin degradation. This result was consistent with the result from Real-time PCR and proved that expression of MMP-9 is a key factor of enhanced metastasis of bladder cancer. Human bladder environment mimicking 3D microfluidic chip allowed more effective observation of cellular interactions when compared to static culture in 2D. The result from this study validated CD44 and MMP-9 to be the key biological factors for migration and metastasis of muscle-invasive bladder cancer. Figure.1 3D microfluidic device mimicking structural environment of bladder cancer This study was supported by the KIST Project. Keywords: Bio-MEMS, Lab on a chip, Cell functionality, biomimetic culture Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in microdevices and microarrays Citation: Lee E, Kwon C, Han H, Park J, Kim Y, Ok M, Seok H and Jeon H (2016). Bladder cancer-on-a-chip for analysis of tumor transition mechanism. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00831 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers' terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Eojin Lee Chunga Kwon Hyungseop Han Jimin Park Yu-Chan Kim Myoung-Ryul Ok Hyun-Kwang Seok Hojeong Jeon Google Eojin Lee Chunga Kwon Hyungseop Han Jimin Park Yu-Chan Kim Myoung-Ryul Ok Hyun-Kwang Seok Hojeong Jeon Google Scholar Eojin Lee Chunga Kwon Hyungseop Han Jimin Park Yu-Chan Kim Myoung-Ryul Ok Hyun-Kwang Seok Hojeong Jeon PubMed Eojin Lee Chunga Kwon Hyungseop Han Jimin Park Yu-Chan Kim Myoung-Ryul Ok Hyun-Kwang Seok Hojeong Jeon Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.