Steel slags, the main waste product from the steel industry, may have several reuse possibilities. Among others, building applications represent a crucial field. However, the potential impact of harmful substances on the environment should be assessed. The aim of this study was to assess the phytotoxicity of steel slags (SS) and concrete mixtures cast with a partial replacement of SS (CSS). Leaching tests were carried out on four SS and four CSS according to EN 12457-2 and UNI EN 15863, respectively. Each leachate was assayed using root elongation tests on 30 seeds of Allium cepa , Cucumis sativus , and Lepidium sativum , respectively, and on 12 bulbs of A. cepa . The latter also allowed the analysis of other macroscopic parameters of toxicity (turgidity, consistency, colour change and root tip shape) and the evaluation of the mitotic index on 20,000 root tip cells per sample. None of the samples induced phytotoxic effects on the organisms tested: all samples supported seedlings emergence, verified by root elongation comparable to, or even greater than, that of the negative controls, and did not affect cell division, as evidenced by mitotic index values. The absence of phytotoxicity demonstrated by the leachates allows SS and SS-derived concrete to be considered as reliable materials suitable for use in civil constructions or in other engineering applications, with economic and environmental advantages, such as the reduction of the final disposal in landfills as well as the consumption of natural resources.

Drinking water quality can be compromised at different stages, from raw water to treated one. This research aimed to evaluate the toxicity and genotoxicity of groundwater contaminated by fluorinated compounds treated in a drinking water treatment plant, through several bioassays. Water samples underwent chemical analyses and were assayed on Daphnia magna , Pseudokirchneriella subcapitata , Allium cepa , human leukocytes and Salmonella typhimurium . Physical–chemical parameters were always within the Italian legislation limits. Water after filtration and disinfection caused slight toxicity in D. magna ; the sample after filtration inhibited the proliferation of P. subcapitata . None of the water samples exerted toxicity in A. cepa . All the analysed samples had genotoxic effects on A. cepa and human leucocytes, while only disinfected water caused mutations in S. typhimurium . A battery composed of tests on D. magna , P. subcapitata , S. typhimurium and A. cepa could represent a useful tool to verify the toxicity/genotoxicity through the water treatment stages and to improve drinking water quality management.

The potential impact of concrete mixtures containing steel slag (SS) as a partial replacement of natural aggregates (NA) on the terrestrial ecosystem was assessed using a battery of plant‐based bioassays. Leaching tests were conducted on four concrete mixtures and one mixture containing only NA (reference concrete). Leachates were tested for phytotoxicity using seeds of Lepidium sativum , Cucumis sativus , and Allium cepa . Emerging seedlings of L. sativum and A. cepa were used to assess DNA damage (comet test). The genotoxicity of the leachates was also analyzed with bulbs of A. cepa using the comet and chromosome aberration tests. None of the samples caused phytotoxic effects. On the contrary, almost all the samples supported the seedlings; and two leachates, one from the SS‐containing concrete and the other from the reference concrete, promoted the growth of C. sativus and A. cepa . The DNA damage of L. sativum and A. cepa seedlings was significantly increased only by the reference concrete sample. In contrast, the DNA damage in A. cepa bulbs was significantly enhanced by the reference concrete but also by that of a concrete sample with SS. Furthermore, all leachates caused an increase in chromosomal aberrations in A. cepa bulbs. Despite some genotoxic effects of the concrete on plant cells, the partial replacement of SS does not seem to make the concrete more hazardous than the reference concrete, suggesting the potential use of SS as a reliable recycled material. Environ Toxicol Chem 2023;42:2193–2200. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Steel slags (SS) are the major waste produced by iron and steel industry. Slags may be reused as recycled materials, instead of natural aggregates (NA), to reduce the final disposal in a landfill and the exploitation of raw materials. However, the reuse of SS may generate a potential release of toxic compounds for the environment and humans. The purpose of this study was to evaluate the toxicity and genotoxicity of SS, in comparison with NA, by using an integrated chemical-biological approach to enable their safe reuse in engineering applications. Leaching solutions from samples were obtained by using short-term leaching tests (CEN EN 12457-2, 2004) usually adopted for the evaluation of waste recovery and final disposal. Chemical analyses of leachates were performed according to the Italian legislation on waste recovery (Ministerial Decree 186/2006). The leaching solutions were assayed by using toxicity test on Daphnia magna. Moreover, mutagenicity/genotoxicity tests on Salmonella typhimurium , Allium cepa , and human leucocytes and fibroblasts were carried out. The releases of pollutants from all samples were within the limits of the Italian legislation for waste recovery. Despite the effects that SS and NA could have on different cells, in terms of toxicity and genotoxicity, globally, SS do not seem to be any more hazardous than NA. This ecotoxicological assessment, never studied before, is important for promoting further studies that may support the decision-making process regarding the use of such types of materials.

In this work, we assess three different methods for the extraction of pectin from waste orange peels, using water as extracting solvent. "Hot-water", Rapid Solid Liquid Dynamic (RSLD) and microwave-assisted extractions have been compared and evaluated in terms of amount and quality of extracted pectin, as well as embodied energy. This analysis provides useful guidelines for pectin production from food waste according to green procedures, enabling the identification of acidic "hot-water" as the most sustainable extraction route.

Steel slags (SS) are the major waste produced by iron and steel industry. Slags may be reused as recycled materials, instead of natural aggregates (NA), to reduce the final disposal in a landfill and the exploitation of raw materials. However, the reuse of SS may generate a potential release of toxic compounds for the environment and humans. The purpose of this study was to evaluate the toxicity and genotoxicity of SS, in comparison with NA, by using an integrated chemical-biological approach to enable their safe reuse in engineering applications. Leaching solutions from samples were obtained by using short-term leaching tests (CEN EN 12457-2, 2004) usually adopted for the evaluation of waste recovery and final disposal. Chemical analyses of leachates were performed according to the Italian legislation on waste recovery (Ministerial Decree 186/2006). The leaching solutions were assayed by using toxicity test on Daphnia magna. Moreover, mutagenicity/genotoxicity tests on Salmonella typhimurium, Allium cepa, and human leucocytes and fibroblasts were carried out. The releases of pollutants from all samples were within the limits of the Italian legislation for waste recovery. Despite the effects that SS and NA could have on different cells, in terms of toxicity and genotoxicity, globally, SS do not seem to be any more hazardous than NA. This ecotoxicological assessment, never studied before, is important for promoting further studies that may support the decision-making process regarding the use of such types of materials.

This paper proposes, for the first time, the use of sewage sludge ash (SSA), obtained by sludge combustion for the stabilization of fly ashes containing heavy metals as Pb and Zn. In particular, the proposed method aims to take advantage of valuable raw materials present in SSA, such as phosphate, silica, and alumina. The stabilization has been explained by the synergic phosphate, carbonation, and pozzolanic reactions together with the Friedel's salt formation. This method to sewage sludge management allows the complete recovery of valuable raw materials otherwise destinated for lanfill. This is virtuous example of the use of waste materials for de-pollution processes.

Human health and well-being are strongly linked to the state of the environment. The high industrial pressure present in the Province of Brescia, located in Northern Italy, produced strong environmental and health concerns. This narrative review of the literature aims at identifying the studies focused on the association between exposure to environmental pollutants and health effects in the population living in this area. Thirteen papers fitted the inclusion criteria: five were focused on the connection among pollutants present in air matrix and health effects, seven on both air and soil, and one on soil. No study investigated the relationship with water pollution. The great variability in the analyzed end-points made it difficult to draw precise conclusions, but the fact that, in almost all the studies, the investigated health effects have a positive association with the exposure to different kinds of pollutants, allows us to hypothesize that the considered population is living in an area where the “environmental pressure” could produce significant health effects in the future.

The European iron and steel industry produces a considerable amount of waste and by-products. Also in Italy the steel slag production is very high. Steel slags may be reused in recycled materials, such as materials for the construction industry, road base and asphalt mixtures, allowing to reduce the final disposal in a landfill. The reuse of this recycled material may generate potential release of toxic compounds for the environment and humans. The aim of this study was to assess the ecotoxicity and genotoxicity of steel slags by using an integrated chemical-biological approach. Chemical analysis of leachates obtained by using short-term leaching tests (UNI EN 12457-2) were performed, to evaluate the waste potential reuse according to the Italian legislation (Ministerial Decree 186/2006). Moreover, solutions obtained from leaching tests were assayed by using ecotoxicity tests on plant and animal organisms and genotoxicity tests on bacteria, plant and human cells. Chemical analyses of the eluates were within the limits of the Italian legislation. The preliminary results of the ecotoxicity and the genotoxicity tests demonstrated that this material has a low toxicity and therefore its potential use as a recycled material.

The reuse of biomass ash as a fertilizer is generally recognized as good practice with several environmental benefits. However, the possible presence of leachable heavy metals in this ash limits the potential extent of its application and the implementation of an appropriate legal framework. For the first time, a method to stabilize wood ash based on the use of other by-products (coal fly ash and rice husk ash) is presented. No commercial chemicals are employed in the procedure. The results show that despite the initial presence of leachable heavy metals in the ash, the final obtained material is stable. In addition, the lowering of pH (from 13.5 to approximately 7.5) due to carbonation reactions and the addition of Ca-rich ash increases the phosphorous availability compared with the starting wood ash and makes the obtained material suitable for use in soil fertilization. The sustainability of the new proposed technology is quantitatively discussed with regard to the differences in embodied energy and CO2 footprint of phosphorous between raw materials and stabilized wood ash. This work shows that the prospects for energy saving and CO2 footprint reduction using stabilized wood ash as a substitute for inorganic commercial P-fertilizers are significant and offer a new way to reach these objectives. The simplicity of the method and the general availability of the by-products employed in the stabilization also render the procedure suitable for applications in developing countries.

This work supports, for the first time, the integrated management of waste materials arising from industrial processes (fly ash from municipal solid waste incineration and coal fly ash), agriculture (rice husk ash), and domestic activities (ash from wood biomass burning in domestic stoves). The main novelty of the paper is the reuse of wood pellet ash, an underestimated environmental problem, by the application of a new technology (COSMOS-RICE) that already involves the reuse of fly ashes from industrial and agricultural origins. The reaction mechanism involves carbonation: this occurs at room temperature and promotes permanent carbon dioxide sequestration. The obtained samples were characterized using XRD and TGA (coupled with mass spectroscopy). This allowed quantification of the mass loss attributed to different calcium carbonate phases. In particular, samples stabilized using wood pellet ash show a weight loss, attributed to the decomposition of carbonates greater than 20%. In view of these results, it is possible to conclude that there are several environmental benefits from wood pellet ash reuse in this way. In particular, using this technology, it is shown that for wood pellet biomass the carbon dioxide conversion can be considered negative.

For the first time in Europe, results of chemical stabilization of municipal solid waste incineration (MSWI) fly ash, realized as a pilot plant, are reported. The stabilization technology is based on the use of only waste and byproduct materials (such as coal fly ash, flue gas desulfurization residues, and rice husk ash). In the frame of the COSMOS-RICE project, a pilot plant was built in Italy, with a capacity of more than 0.5 tons of MSWI fly ash/day. The stability of the material obtained was investigated by the evaluation of the pH dependency of the leaching of metals, 6 months after the treatment. The pH dependence of heavy metal solubility was described in terms of unified leaching curves, comparing first-time leaching data of MSWI fly ash from different geographical locations (Italy, Portugal, Denmark, and China), using a polynomial function. Results confirmed that COSMOS-RICE technology can be a promising path for obtaining a stabilized product with likely practical applications, avoiding costly and unsustainable landfilling.

Ashes derived from biomass combustion and used as soil fertilizers can generate negative environmental and human health risks, related to leaching of heavy metals and other putative toxic elements. Tree wood ash composition may vary depending on geographical location and surrounding industrial processes. In this study, we evaluated the biotoxicity of lixiviated tree wood ash samples from trees of the Ash (Fraxinus), Cherry (Pronus), Hazel (Corylus), and Black locust (Robinia) genus collected in an industrialized region in Northern Italy. Elemental chemical analysis of the samples was performed by total reflection X-ray fluorescence technique and their biotoxicity was assessed in zebrafish (Danio rerio) embryos. Ashes from Ash, Cherry, and Hazel trees, but not Black locust trees, had a high concentration of heavy metals and other putative toxic elements. Accordingly, a dose-dependent increase in mortality rate and morphological and teratogenic defects was observed in zebrafish embryos treated with lixiviated Ash, Cherry, and Hazel tree wood samples, whereas the toxicity of Black locust tree wood ashes was negligible. In conclusion, lixiviated wood ashes from different plants show a different content of toxic elements that correlate with their biotoxic effects on zebrafish embryos. Tree wood ashes derived from biomass combustion may represent a potential risk for the environment and human health.

This paper is devoted to the chemical analysis of contaminated soils of India and the rice grown in the same area. Total reflection X-ray fluorescence spectroscopy is a well-established technique for elemental chemical analysis of environmental samples, and it can be a useful tool to assess food safety. Metals uptake in rice crop grown in soils from different areas was studied. In this work soil, rice husk and rice samples were analyzed after complete solubilization of samples by microwave acid digestion. Heavy metals concentration detected in rice samples decreases in the following order: Mn > Zn > Cu > Ni > Pb > Cr. The metal content in rice husk was higher than in rice. This study suggests, for the first time, a possible role of heavy metals filter played by rice husk. The knowledge of metals sequestration capability of rice husk may promote some new management practices for rice cultivation to preserve it from pollution.

The Stabilization of heavy metals from municipal solid waste incineration (MSWI) fly ash by rice husk ash (RHA) is under intense study as an effective strategy to recover and reuse industrial and agricultural waste together. We compare the metal entrapment performances of RHA from different Asian rice sources – namely from Japonica rice grown in Italy and Indica rice grown in India – Physicochemical and morphological characterization of the final stabilized material show that the same thermal treatment may result in marked structural differences in the silica contained in the two RHA. Remarkably, one of them displays a crystalline silica content, although obtained by a thermal treatment below 800 °C. We also find that the presence of an alkali metal ion (potassium) in the rice husk plays a crucial role in the attainment of the final silica phase. These physicochemical differences are mirrored by different stabilization yields by the two RHA.

A new technology was recently developed for municipal solid waste incineration (MSWI) fly ash stabilization, based on the employment of all waste and byproduct materials. In particular, the proposed method is based on the use of amorphous silica contained in rice husk ash (RHA), an agricultural byproduct material (COSMOS-RICE project). The obtained final inert can be applied in several applications to produce “green composites”. In this work, for the first time, a process for pre-treatment of rice husk, before its use in the stabilization of heavy metals, based on the employment of Instant Pressure Drop technology (DIC) was tested. The aim of this work is to verify the influence of the pre-treatment on the efficiency on heavy metals stabilization in the COSMOS-RICE technology. DIC technique is based on a thermomechanical effect induced by an abrupt transition from high steam pressure to a vacuum, to produce changes in the material. Two different DIC pre-treatments were selected and thermal annealing at different temperatures were performed on rice husk. The resulting RHAs were employed to obtain COSMOS-RICE samples, and the stabilization procedure was tested on the MSWI fly ash. In the frame of this work, some thermal treatments were also realized in O2-limiting conditions, to test the effect of charcoal obtained from RHA on the stabilization procedure. The results of this work show that the application of DIC technology into existing treatment cycles of some waste materials should be investigated in more details to offer the possibility to stabilize and reuse waste.

A new sustainable method for heavy metals stabilization, based on the use of all waste, is proposed. No chemicals are involved in the reaction. The stabilization process, that is named COSMOS, also shows a great potential for CO2 sequestration.