ObjectivesSarcopenia and disability represent significant concerns impacting the health of older people. This study aimed to explore the bidirectional relationship between sarcopenia and disability in Chinese older people.MethodsThis study recruited older people ≥60 years old from the China Health and Retirement Longitudinal Study. In phase I, the study analyzed the relation between disability and subsequent sarcopenia using multinomial logistic regression models. Conversely, in phase II, the study assessed whether sarcopenia was associated with future disability using binary logistic regression models.ResultsIn phase I, 65 (16.80%) new cases of possible sarcopenia, 18 (4.65%) cases of sarcopenia, and 9 (2.33%) cases of severe sarcopenia were observed in the disabled older people and 282 (10.96%) new cases of possible sarcopenia, 97 (3.77%) cases of sarcopenia, 35 (1.36%) cases of severe sarcopenia were observed in the older people without disability. The OR (95% CI) for sarcopenia in older disabled individuals compared to those without disability was 1.61 (1.25–2.07). Adjusting for all covariates in 2011, the OR (95% CI) value for disabled individuals vs. those without disability was 1.35 (1.02–1.79). Subgroup analyses showed that disabled participants aged < 80 years were more likely to have sarcopenia (OR = 1.42, 95% CI: 1.07–1.89), and the risk of sarcopenia did not differ significantly between sex subgroups. In phase II, 114 cases (33.83%) in the possible sarcopenia patients, 85 cases (28.91%) in the sarcopenia patients, 23 cases (35.94%) in the severe sarcopenia patients, and 501 cases (16.10%) in the individuals without sarcopenia showed symptoms of disability. The OR (95% CI) for disability was 2.66 (2.08–3.40) in the possible sarcopenia patients, 2.12 (1.62–2.77) in the sarcopenia patients, and 2.92 (1.74–4.91) in the severe sarcopenia patients compared with the no sarcopenia patients. After adjusting for all covariates in 2011, the OR (95% CI) values were 2.21 (1.70–2.85) in the possible sarcopenia patients, 1.58 (1.14–2.19) in the sarcopenia patients, and 1.99 (1.14–3.49) in the severe sarcopenia patients, as compared to the older people without sarcopenia. Subgroup analyses showed that compared with men, women with possible sarcopenia had a higher risk of disability (OR = 2.80, 95% CI: 1.98–3.97). In addition, participants aged < 80 years with sarcopenia or severe sarcopenia s were more likely to have disability (OR = 2.13, 95% CI: 1.52–2.98; OR = 2.98, 95% CI: 1.60–5.54).ConclusionThe occurrence of disability increase the risk of sarcopenia in the older people, and baseline sarcopenia predicts the future disability in older people.

Background Under the general trend of global aging, geriatric comorbidity is increasingly common, which may have some impact on the quality of life of the older people. Self-management can effectively improve patient compliance, subjective initiative, and improve patient quality of life. However, the present situation of self-management in different old people is different. Therefore, this study classifies older co-morbid patients through potential profiling analysis, understands the category characteristics of self-management level of older co-morbid patients, and discusses the influencing factors of self-management level of different categories of older co-morbid patients, which can provide reference for personalized intervention programs for different comorbidity characteristics of elderly people in the future. Method Through a cross-sectional study, 616 cases of older co-morbid patients in three districts of Zhengzhou City, Henan Province, were selected as survey subjects by using the whole cluster sampling method. The General Information Questionnaire, Chronic Disease Self-Management Scale, Health Literacy Scale, Electronic Health Literacy Scale, Collaborative Social Support Scale, and Health Empowerment Scale were used to conduct the survey. Results The result of LPA shows that the self-management characteristics of older co-morbid patients should be classified into 3 categories: good self-management (19.4%), medium self-management(27.9%), and low self-management (52.7%). The results of multivariate logistic regression analyses show that literacy, religiosity, health literacy, e-health literacy, appreciative social support, and health empowerment are influential factors for self-management among older co-morbid patients (p < 0.05). Conclusion There is obvious heterogeneity in the self-management level of older co-morbid patients. It is recommended that healthcare professionals give targeted interventions for their weaknesses according to the self-management characteristics of different categories of patients in order to enhance the self-management level of this population and improve their quality of life.

Branch length is an important plant architecture trait in cotton (Gossypium) breeding. Development of cultivars with short branch has been proposed as a main object to enhance cotton yield potential, because they are suitable for high planting density. Here, we report the molecular cloning and characterization of a semi-dominant quantitative trait locus, Short Branch Internode 1(GhSBI1), which encodes a NAC transcription factor homologous to CUP-SHAPED COTYLEDON 2 (CUC2) and is regulated by microRNA ghr-miR164. We demonstrate that a point mutation found in sbi1 mutants perturbs ghr-miR164-directed regulation of GhSBI1, resulting in an increased expression level of GhSBI1. The sbi1 mutant was sensitive to exogenous gibberellic acid (GA) treatments. Overexpression of GhSBI1 inhibited branch internode elongation and led to the decreased levels of bioactive GAs. In addition, gene knockout analysis showed that GhSBI1 is required for the maintenance of the boundaries of multiple tissues in cotton. Transcriptome analysis revealed that overexpression of GhSBI1 affects the expression of plant hormone signalling-, axillary meristems initiation-, and abiotic stress response-related genes. GhSBI1 interacted with GAIs, the DELLA repressors of GA signalling. GhSBI1 represses expression of GA signalling- and cell elongation-related genes by directly targeting their promoters. Our work thus provides new insights into the molecular mechanisms for branch length and paves the way for the development of elite cultivars with suitable plant architecture in cotton.

Background In the face of the serious challenges posed by the high prevalence of chronic diseases among the elderly and the coexistence of multiple chronic diseases, the health problems of community-dwelling elderly people' with multimorbidity require urgent attention. The comorbidity management model is being explored and practiced. Disease experience, as self-reported health information from patients, is expected to become a key component of the patient-centered community multimorbidity management model. Objective This study aimed to gain a deeper understanding of the real disease experience of community-dwelling elderly people with multimorbidity and provide a basis for personalized health management and supportive strategies of the group. Methods This study used purposive sampling and maximum difference sampling to select eligible interviewees from four communities under the jurisdiction of a community health service center in Zhengzhou City from August to September in 2022. Based on the principle of information saturation, 20 subjects were ultimately included. Based on the research objective, literature review, and expert consultation results, the preliminary interview outline was formulated, and the final interview outline was determined after interviewing 2 patients. Semi-structured interviews were conducted with the study participants, guided by the phenomenological research methodology. Golaizzi analysis was used to analyse the data. Results Four themes and eleven sub-themes had been extracted from this study, including mainly long-standing symptomatic distress (physical dysfunction, fatigue, sleep disorders, and pain), negative psychological experiences (feelings of helplessness and worry about future life, reduced sense of social value and self-identity, and loneliness with altered social activities), positive mindset of coexisting harmoniously with multimorbidity (being grateful for being satisfied, and positively adapting), and aspirations in times of adversity (hoping for improvement in functioning, and seeking multi-directional social support) . Conclusion Physical dysfunction, sleep disturbances, fatigue, and pain are the most concerning health disturbances for community-dwelling elderly people with multimorbidity. The symptom management and multiple social support of the multimorbidity need to be strengthened. In the future, it is expected that multidimensional data would be used for dynamic health assessment to explore the common and individual patterns of multimorbidity, and to achieve precise management and intervention of elderly multimorbidity in the community.

世界卫生组织倡议实施整合照护模式以支持全民健康老龄化,健康是人类适应生态位的最终结局指标.该研究基于整合视角,系统梳理了农村养老生态系统中服务资源的供给现状,并结合生态位理论中生物与资源、环境的动态交互机制,构建了以家庭资源、医疗服务、机构供给、社会支持、政策保障为框架的农村养老生态资源供给模型,并倡议进一步整合养老物质资源供给、优化养老服务支持网络、完善养老政策保障体系,以积极应对农村老龄化、失能化,提升老年人的健康水平.

对国内外智慧养老系统的构建及应用效果进行综述,提出我国今后进行智慧养老系统设计的展望,旨在为我国智慧养老的发展提供参考.

通过梳理六字诀呼吸操、鸟戏呼吸操、经络呼吸操、呼吸导引操、吐纳呼吸操、五音呼吸操6种中医呼吸操在临床护理实践中的特点、应用方式及效果,明确中医呼吸操的应用情况及不足,以期为今后中医呼吸操的进一步研制和推广应用提供借鉴.

BACKGROUND:Phospholipases As (PLAs) are acyl hydrolases that catalyze the release of free fatty acids in phospholipids and play multiple functions in plant growth and development. The three families of PLAs are: PLA1, PLA2 (sPLA), and patatin-related PLA (pPLA). The diverse functions that pPLAs play in the growth and development of a broad range of plants have been demonstrated by prior studies.METHODS:Genome-wide analysis of the pPLA gene family and screening of genes for expression verification and gene silencing verification were conducted. Additionally, pollen vitality testing, analysis of the pollen expression pattern, and the detection of POD, SOD, CAT, MDA, and H2O2 were performed.RESULT:In this study, 294 pPLAs were identified from 13 plant species, including 46 GhpPLAs that were divided into three subfamilies (I-III). Expression patterns showed that the majority of GhpPLAs were preferentially expressed in the petal, pistil, anther, and ovule, among other reproductive organs. Particularly, GhpPLA23 and GhpPLA44, were found to be potentially important for the reproductive development of G. hirsutum. Functional validation was demonstrated by VIGS which showed that reduced expression levels of GhpPLA23 and GhpPLA44 in the silenced plants were associated with a decrease in pollen activity. Moreover, a substantial shift in ROS and ROS scavengers and a considerable increase in POD, CAT, SOD, and other physiological parameters was found out in these silenced plants. Our results provide plausibility to the hypothesis that GhpPLA23 and GhpPLA44 had a major developmental impact on cotton reproductive systems. These results also suggest that pPLAs are important for G. hirsutum's reproductive development and suggest that they could be employed as potential genes for haploid induction.CONCLUSIONS:The findings of the present research indicate that pPLA genes are essential for the development of floral organs and sperm cells in cotton. Consequently, this family might be important for the reproductive development of cotton and possibly for inducing the plant develop haploid progeny.

Abstract Background Under the general trend of global aging, comorbidity among the elderly is becoming more and more common, which may have a certain impact on the quality of life of the elder. There is evidence that self-management and health empowerment are positively correlated with quality of life. However, limited research has explored the potential mechanism among them. Therefore, the purpose of this study is to explore the interaction path and mechanism among health empowerment, self-management and quality of life of elderly comorbid patients, and to provide theoretical basis for formulating intervention programs to improve their quality of life in the later stage. Objective To explore the relationship among health empowerment, self-management and quality of life of elderly patients with comorbidity. Methods 309 elderly patients with comorbidity in Zhengzhou City, Henan Province were investigated by questionnaire, including Health Empowerment Scale, Chronic Disease Self-management Scale and SF-12 Scale. Results The score of health empowerment, self-management and quality of life of the elderly patients with comorbidity were (92.18±8.917), (136.58±12.42) and (89.30±8.72) respectively. Health empowerment, self-management and quality of life of elderly patients with comorbidity were positively correlated (P < 0.01). The direct effect of health empowerment on quality of life is 0.493, the indirect effect is 0.207, the total effect is 0.724, and the intermediary effect accounts for 28.75% of the total effect. Self-management plays a partial intermediary role between health empowerment and quality of life. Conclusion The health empowerment, self-management and quality of life of elderly comorbid patients need to be further improved. The self-management of elderly comorbid patients plays a partial intermediary role between health empowerment and quality of life, and the quality of life of patients can be effectively improved by improving their health empowerment and self-management ability.

BACKGROUND:Cytokinin oxidase/dehydrogenase (CKX) plays a vital role in response to abiotic stress through modulating the antioxidant enzyme activities. Nevertheless, the biological function of the CKX gene family has yet to be reported in cotton.RESULT:In this study, a total of 27 GhCKXs were identified by the genome-wide investigation and distributed across 18 chromosomes. Phylogenetic tree analysis revealed that CKX genes were clustered into four clades, and most gene expansions originated from segmental duplications. The CKXs gene structure and motif analysis displayed remarkably well conserved among the four groups. Moreover, the cis-acting elements related to the abiotic stress, hormones, and light response were identified within the promoter regions of GhCKXs. Transcriptome data and RT-qPCR showed that GhCKX genes demonstrated higher expression levels in various tissues and were involved in cotton's abiotic stress and phytohormone response. The protein-protein interaction network indicates that the CKX family probably participated in redox regulation, including oxidoreduction or ATP levels, to mediate plant growth and development. Functionally identified via virus-induced gene silencing (VIGS) found that the GhCKX14 gene improved drought resistance by modulating the antioxidant-related activitie.CONCLUSIONS:In this study, the CKX gene family members were analyzed by bioinformatics, and validates the response of GhCKX gene to various phytohormone treatment and abiotic stresses. Our findings established the foundation of GhCKXs in responding to abiotic stress and GhCKX14 in regulating drought resistance in cotton.

Abstract Background Phospholipases As (PLAs) are acyl hydrolase that can catalyze the release of free fatty acids in phospholipids and play multiple functions in plant growth and development. PLAs can be divided into three families, PLA1, PLA2 (sPLA) and patatin-related PLA (pPLA). Previous studies have elucidated the versatile roles of pPLAs in growth and development of many plants.Methods Genome-wide analysis of the pPLA family and screening of genes for expression verification and gene silencing verification. After gene silencing, analysis of pollen expression pattern, pollen vitality test and POD, SOD, CAT, MDA and H2O2 detection.Result In this study, a total of 294 pPLAs were identified from 13 species, including 46 GhpPLAs divided into three subfamilies (I-III-γ). Expression pattern analysis revealed that most of GhpPLAs were preferentially expressed in the reproductive organs (petal, pistil, anther and ovule), especially two genes (GhpPLA23 and GhpPLA44) were deduced that may affect the reproductive development of G. hirsutum. VIGS experiment of GhpPLA23 and GhpPLA44 showed that the pollen activity decreased with their lower expression levels in the silenced plants. The contents of POD, CAT, SOD and other physiological indicators were significantly increased, while ROS and ROS scavengers were significantly changed after genes silencing, indicating that these two genes had important effects on cotton reproductive development. These findings suggest that pPLAs play a crucial role in reproductive development of G. hirsutum and show the potential to be utilized as candidate genes for haploid inducing.Conclusions In this study, pPLA genes play an important role in the reproductive organs of cotton, affecting the development of flower organs and sperm cells, so this family may play an important role in the reproductive development progress of cotton, and even in inducing cotton to produce haploid.

Abstract Background Under the general trend of global aging, comorbidity among the elderly is becoming more and more common, which may have a certain impact on the quality of life of the elder. There is evidence that self-management and health empowerment are positively correlated with quality of life. However, limited research has explored the potential mechanism among them. Therefore, the purpose of this study is to explore the interaction path and mechanism among health empowerment, self-management and quality of life of elderly comorbid patients, and to provide theoretical basis for formulating intervention programs to improve their quality of life in the later stage. Objective To explore the relationship among health empowerment, self-management and quality of life of elderly patients with comorbidity. Methods 309 elderly patients with comorbidity in Zhengzhou City, Henan Province were investigated by questionnaire, including Health Empowerment Scale, Chronic Disease Self-management Scale and SF-12 Scale. Results The score of health empowerment, self-management and quality of life of the elderly patients with comorbidity were (92.18±8.917), (136.58±12.42) and (89.30±8.72) respectively. Health empowerment, self-management and quality of life of elderly patients with comorbidity were positively correlated (P < 0.01). The direct effect of health empowerment on quality of life is 0.493, the indirect effect is 0.207, the total effect is 0.724, and the intermediary effect accounts for 28.75% of the total effect. Self-management plays a partial intermediary role between health empowerment and quality of life. Conclusion The health empowerment, self-management and quality of life of elderly comorbid patients need to be further improved. The self-management of elderly comorbid patients plays a partial intermediary role between health empowerment and quality of life, and the quality of life of patients can be effectively improved by improving their health empowerment and self-management ability.

对Swanson理论的概念、理论框架及其在临床护理、护理教育和护理科研中的应用现状进行综述,提出Swanson理论应用过程中存在的问题及针对性的建议,以期为Swanson关怀理论的推广应用提供参考依据.

The Jumonji C (JmjC) domain-containing protein family, an important family of histone demethylase in plants, can directly reverse histone methylation and play important roles in various growth and development processes. In the present study, 51 JmjC genes (GhJMJs) were identified by genome-wide analysis in upland cotton (Gossypium hirsutum), which can be categorized into six distinct groups by phylogenetic analysis. Extensive syntenic relationship events were found between G. hirsutum and Theobroma cacao. We have further explored the putative molecular regulatory mechanisms of the JmjC gene family in cotton. GhJMJ24 and GhJMJ49 were both preferentially expressed in embryogenic callus compared to nonembryogenic callus in cotton tissue culture, which might be regulated by transcription factors and microRNAs to some extent. Further experiments indicated that GhJMJ24 and GhJMJ49 might interact with SUVH4, SUVH6, DDM1, CMT3, and CMT1 in the nucleus, potentially in association with demethylation of H3K9me2. Taken together, our results provide a foundation for future research on the biological functions of GhJMJ genes in cotton, especially in somatic embryogenesis in cotton tissue culture, which is crucial for the regeneration of transgenic plants.

Filamin protein is characterized by an N-terminal actin-binding domain that is followed by 24 Ig (immunoglobulin)-like repeats, which act as hubs for interactions with a variety of proteins. In humans, this family has been found to be involved in cancer cell invasion and metastasis and can be involved in a variety of growth signal transduction processes, but it is less studied in plants. Therefore, in this study, 54 Filamin gene family members from 23 plant species were investigated and divided into two subfamilies: FLMN and GEX2. Subcellular localization showed that most of the Filamin gene family members were located in the cell membrane. A total of 47 Filamin gene pairs were identified, most of which were whole-genome copies. Through the analyses of cis-acting elements, expression patterns and quantitative fluorescence, it was found that GH_ A02G0519 and GH_ D02G0539 are mainly expressed in the reproductive organs of upland cotton, and their interacting proteins are also related to the fertilization process, whereas GH_A02G0216 and GH_D02G0235 were related to stress. Thus, it is speculated that two genes of the GEX2 subfamily (GH_A02G0519 and GH_D02G0539) may be involved in the reproductive development of cotton and may affect the fertilization process of cotton. This study provides a theoretical basis for the further study of the cotton Filamin gene family.

在对整合照护发展现状、内涵分析的基础上,回顾了英国、澳大利亚、荷兰、日本、泰国、爱尔兰6个国家的整合照护模式及整合照护实践的阻碍和促进因素,为完善我国养老服务体系提供参考依据.