目的:建立菊花药材中6种化合物(绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸、木犀草素、山柰酚以及金合欢素)的HPLC含量测定方法及其指纹图谱检测方法,并对所收集到的市售5种药用菊花样品利用主成分分析获取能反映不同菊花样本间基原远近的关键成分.方法:含量测定部分采用YMC-Park ODS-AQ C1s(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.01％磷酸为流动相梯度洗脱,流速0.8 mL·min-1,检测波长330 nm,柱温25℃;结合SPSS 16数据分析软件及SIMCA-P主成分分析软件对含量测定的数据进行分析,筛选出对菊花基原判定影响最大的特征成分组.结果:含量测定方法学验证结果良好,并且通过含量测定结果的数据分析,确立了与菊花基原判别最相关的特征成分组为木犀草素及木犀草苷.结论:本研究所建立的含量测定方法可以用于菊花药材中6种成分的定量分析,主成分分析所筛选出的基原远近的决定性成分因子,能大体上将不同来源的菊花样品按基原归类,为市售菊花基原鉴定提供参考依据.

目的:以中医辨证论治为基础,研究生地黄对急性肺损伤/急性呼吸窘迫综合征(acute lung injury/acute respiratory distress syndrome,ALI/ARDS)的干预作用.方法:将大鼠分为对照组、模型组、生地黄预防组、生地黄治疗组,通过气管注射脂多糖(LPS)制备大鼠ALI/ARDS模型,酶联免疫法(ELISA)测定支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白细胞介素-6(interleukin-6,IL-6)含量的变化,考马斯亮蓝法测定BALF中总蛋白含量,BALF细胞悬浮液离心涂片Giemsa染色后光镜下细胞分类计数,H.E.染色肺部组织并通过光镜观察其形态学变化,分析生地黄水提物对ALI的保护作用.结果:生地黄干预组BALF中TNF-α、IL-6含量明显降低(P＜0.0),蛋白含量明显降低(P＜0.0),中性粒细胞数(Polymorphonuclear cells,PMN)和炎症细胞总数明显降低(P＜0.0),肺部病理损伤减轻.结论:生地黄对ALI可能具有保护作用,对ALI/ARDS可能具有潜在的治疗价值.

Objective To investigate the effects of Moutan cortexon lipopolysaccharide-induced acute lung injury (ALI) in rats,and explore the underlying mechanism against ALI. Methods 55 wistar rats were randomly divided into control group, model group,positive drug group,treatment group and prevention group,the animal model of ALI was established by intratracheal administration oflipopolysaccharide. The percentage of neutrophils﹑the concentration of protein exudation﹑cytokine ( TNF-α、IL-6 ) levels in bronchoalveolar lavage fluid ( BALF ) and histopathological changes in lung tissue were observed 12 h after LPS administration. Results Moutan cortex and dexamethasone can effectively reduce the PMN percentage and&nbsp;protein concentration(P<0. 01), lower the level of TNF-α﹑IL-6 (P<0. 05 or P<0. 01) ,significantly improve the alveolar structure damage ﹑ pulmonary edema and inflammatory cells infiltration, etc. Conclusion Moutan cortex can protect lung through inhibiting the inflammation reaction of ALI and alleviating pulmonary inflammation.

Objective: To study the chemical constituents from Shuanghuanglian poder for injection. Methods: The constituents were isolated and purified by silica gel,ODS,and Sephadex LH- 20 column chromatographies as well as HPLC. Their chemical structures were elucidated on the basis of spectral data. Results: The compounds were isolated from the MeOH fraction of Shuanghuanglian poder for injection and identified as Phillygenin( 1),Oroxylin- A( 2),( +)-epipinoresino- 4'- β- D-- glueoside( 3),Isolariciresinol( 4),oroxylinA- 7- O- β- D- glucuronide( 5),Chrysin- 7- glucuronide methyl ester( 6). Caffeicacide- thylester( 7). Conclusion: Compounds 1 ~ 7 are isolated from Shuanghuanglian injection for the first time,and compounds 6 and 7 have not been isolated from Scutellariae Radix,Forsythiae Fructusand and Lonicera japonicae Flos.