通过对《刘涓子鬼遗方》治疗痈疽相关内容的整理,从病名病机、辨证治法、遣方用药等方面剖析了刘涓子治疗痈疽的治疗思想和用药经验.刘涓子根据痈疽的发病特点、症状特征及病势过程分期论治,认为痈疽发病之病理因素为火热,主要发病机理为热毒郁滞、血壅内腐,故以清热解毒为基本治疗大法,时刻不忘祛火有路,使火散热消;组方以“托”为主重用黄芪,营卫、气血同调,内外兼治,以多种剂型配合灵活的用药思路,为当代外科临床与学术发展提供了宝贵经验.

泄泻是临床的常见病、多发病,其命名特点随着时代的变迁,也发生了变化.先秦两汉时期,多以"形容词+泄"或与泄泻发生相关的脏腑命名;隋唐时期,多以所泻下物的不同来命名;宋金元时期,多以"三因"命名并确立了"泄泻"这一专有病名;明清时期,统一泄泻的病名及完善与痢疾的鉴别;现代中医内科学则以有无脓血便和里急后重分辨各种肠道疾病为泄泻还是痢疾.

通过阐述“通补兼施”法之机理、“通补兼施”法之具体应用以介绍吴承艳教授治疗多囊卵巢综合征性不孕症经验.认为“虚”与“不通”是导致该病的主要原因,“虚”者包括脾与肾的亏虚,“不通”者包括痰湿、湿热、寒湿、瘀血、肝郁阻滞不通,故在治疗时虚者补之、不通者通之,“通补兼施”具体应用抓住辨证、分期、兼症三点,“辨证”重在辨证候之虚实,“分期”重在明各生理分期之特点,“兼症”重在察主兼症之联系,参考三者灵活运用“通”“补”.文末举验案一则,以供思路借鉴.

Objective To study the mechanisms of breast milk in preventing necrotizing enterocolitis ( NEC) through determining the impact of breast milk on the expression of NF-κB p65 in Caco-2 cells model with hypoxia/reoxygenation injury.Methods The study had control groups and model groups.They were divided into three groups( MEM,5%BM and 5%BMb) .We added conventional cul-ture fluid,5%breast milk supernatant fluid and 5%breast milk supernatant fluid boiled into the three groups respectively after the model was builded.All cells continually cultured six hours, then the expression of NF-κB p65 in Caco-2 cells were detected with RT-PCR and immunofluorescence method.Results RT-PCR method showed that in control groups and model groups 5%breast milk supernatant fluid boiled or unboiled could inhibit the expression of NF-κB p65 mRNA(P<0.01).Compared with 5%breast milk supernatant fluid boiled,the inhibitory effect of 5% breast milk supernatant fluid was better in model groups(P<0.05).Immunofluorescence method showed that the expression of NF-κB p65 in the cytoplasm of Caco-2 cells with hypoxia/reoxygenation injury was activated into the nu-cleus,which could be inhibited by 5%breast milk supernatant fluid boiled or unboiled,and 5%breast milk supernatant fluid was better. Conclusion Fresh breast milk can prevent intestinal epithelial cells from ischemia reperfusion injury by inhibiting the expression of NF-κB p65,which may be one of the mechanisms of breast milk preventing NEC.