Objective To identify core acupoint patterns and elucidate the molecular mechanisms of acupuncture for primary depressive disorder(PDD)through data mining and network analy-sis. Methods A comprehensive literature search was conducted across PubMed,Embase,Ovid Technologies(OVID),Web of Science,Cochrane Library,China National Knowledge Infras-tructure(CNKI),China National Knowledge Infrastructure Database(VIP),Wanfang Data,and SinoMed Database from database foundation to January 31,2025,for clinical studies on acupuncture treatment of PDD.Descriptive statistics,high-frequency acupoint analysis,de-gree and betweenness centrality evaluation,and core acupoint prescription mining identified predominant therapeutic combinations for PDD.Network acupuncture was used to predict therapeutic target for the core acupoint prescription.Subsequent protein-protein interaction(PPI)network and molecular complex detection(MCODE)analyses were conducted to iden-tify the key targets and functional modules.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses explored the underlying biological mechanisms of the core acupoint prescription in treating PDD. Results A total of 57 acupoint prescriptions underwent systematic analysis.The core thera-peutic combinations comprised Baihui(GV20),Yintang(GV29),Neiguan(PC6),Hegu(LI4),and Shenmen(HT7).Network acupuncture analysis identified 88 potential therapeutic tar-gets(79 overlapping with PDD),while PPI network analysis revealed central regulatory nodes,including interleukin(IL)-6,IL-1β,tumor necrosis factor(TNF)-α,toll-like receptor 4(TLR4),IL-10,brain-derived neurotrophic factor(BDNF),transforming growth factor(TGF)-β1,C-X-C motif chemokine ligand 10(CXCL10),mitogen-activated protein kinase 3(MAPK3),and ni-tric oxide synthase 1(NOS1).MCODE-based modular analysis further elucidated three func-tionally coherent clusters:inflammation-homeostasis(score=6.571),plasticity-neurotrans-mission(score=3.143),and oxidative stress(score=3.000).GO and KEGG analyses demon-strated significant enrichment of the MAPK,phosphoinositide 3-kinase/protein kinase B(PI3K/Akt),and hypoxia-inducible factor(HIF)-1 signaling pathways.These mechanistic in-sights suggested that the antidepressant effects mediated through mechanisms of neuroin-flammatory regulation,neuroplasticity restoration,and immune-oxidative stress homeosta-sis. Conclusion This study reveals that acupuncture alleviates depression through a multi-level mechanism,primarily involving the neuroinflammation suppression,neuroplasticity enhancement,and oxidative stress regulation.These findings systematically clarify the underlying mechanisms of acupuncture's antidepressant effects and identify novel therapeu-tic targets for further mechanistic research.

Objective This study aimed to explore the clinical effect of acupuncture therapy for menstruation regulation and pregnancy promotion on thin endometrium in the real world. Design This study is a single-center pragmatic randomized controlled trial blinded to the statisticians. Using the "blockrand" software package, based on the age (>= 35, <35), 37 patients were randomized into an intervention group (19 cases) and a control group (18 cases). After reassignment regarding patient preference, 21 patients were included in the intervention group and 16 in the control group. Setting The trial was executed in the Specialty Outpatient Clinic, Acupuncture-Moxibustion Hospital of China Academy of Chinese Medical Sciences, from March 1, 2019, to September 30, 2020. Participants The study included 37 patients with thin endometrium and without previous acupuncture treatment. Intervention The intervention group was administered acupuncture for menstruation regulation and pregnancy promotion and a small-dose of progynova (2 mg daily), while the control group was administered a large-dose of progynova (4 mg daily). Interventions started from Day 5 of menstruation until the end of ovulation under B-ultrasound monitoring. The intervention lasted for three menstrual cycles. Measurements Primary outcomes were changes in endometrial thickness between baseline and after intervention completion and the difference between the two groups after intervention. The secondary outcomes were endometrial and subendometrial blood flow, serum estradiol levels, menstrual conditions, and adverse reactions. Results (1) Comparison of each indicator before and after intervention completion in the two groups: in the intervention group, the differences were significant in endometrial thickness, menstrual score, estradiol (E2) level in ovulatory period, the pulsatility index (PI) and resistance index (RI) of uterine artery, the ratio of peak systolic velocity to end-diastolic velocity (S/D), the endometrial vascular index (VI), flow index (FI), and vascular flow index (VFI) and volume (P < 0.01). In the control group, significant changes were observed in endometrial thickness, menstrual score, and E2 before and after the intervention (P < 0.05), and no differences were observed in uterine artery PI, RI, S/D and endometrial VI, FI, VFI, and volume (P > 0.05). Compared with the control group, the intervention group showed significant differences in endometrial thickness, menstrual score, E2, uterine artery PI, RI, S/D, and endometrial VI, FI, VFI, and volume after intervention (P < 0.01). No adverse reactions were reported in the intervention group. In contrast, the control group had two cases of nausea and gastrointestinal discomfort after medication, eight cases of breast distention during medication, and one case of breast nodules enlarged by 1 cm after trial completion. Conclusion Acupuncture for menstrual regulation and pregnancy promotion combined with a low dose of estrogen can effectively improve endometrial receptivity in patients with thin endometrium, which may be related to stimulating endogenous hormone secretion, increasing the endometrial thickness and improving blood flow. Trial registration: AMCTR-IPR-19000295. (c) 2025 World Journal of Acupuncture - Moxibustion House. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)

目的:系统评价行体外受精-胚胎移植过程中针灸对卵巢低反应(POR)患者妊娠结局和卵巢功能有效性的疗效.方法:计算机检索国家知识基础设施数据库(CNKI)、中文科技期刊数据库(CCD)、中国生物医学文献数据库(CBM)、中国学术期刊数据库(CSPD)、PubMed、Cochrane Library数据库,检索针灸治疗POR的随机对照试验(RCT),检索时限为建库至2021年8月31日.采用RevMan 5.4软件对纳入研究进行偏倚风险评价和数据分析,并进行GRADE证据质量评价.结果:共纳入8个RCT,包括537例患者.Meta分析结果显示:与体外受精-胚胎移植(IVF-ET)组比较,针灸+IVF-ET组可提高POR患者临床妊娠率(RR=1.58,95％CI为1.03～2.42,P=0.04),增加获卵数(MD=1.16,95％CI为0.47～1.85,P=0.001)和窦卵泡数(MD=0.93,95％CI为0.06～1.8,P=0.04),降低促卵泡素(FSH)水平(SMD=-0.29,95％CI为-0.50～-0.08,P=0.007),但2组在提高受精率、优胚率、降低促黄体素(LH)水平、提高抗米勒管激素(AMH)水平、降低促性腺激素(Gn)用量上差异均无统计学意义.结论:现有证据表明,针灸可提高接受IVF-ET助孕的POR患者临床妊娠率,增加获卵数和窦卵泡数,降低POR患者的FSH水平.由于纳入研究数量较少且存在一定的偏倚风险,本研究结论后续仍需要高质量研究进行验证.

目的 基于中国临床试验注册中心(ChiCTR)和美国临床试验注册中心(ClinicalTrials.gov)数据库,分析中医药治疗卵巢功能减退性疾病研究注册现状和不足,为今后相关临床试验注册和开展提供参考.方法 系统检索ChiCTR和ClinicalTrials.gov建库至2021年12月22日收录的中医药治疗卵巢功能减退性疾病注册临床试验,由专人进行试验筛选和数据提取,采用Excel2019录入数据并进行统计分析.结果 最终纳入41项中医药治疗卵巢功能减退性疾病注册临床试验,其中卵巢储备功能减退研究17项、早发性卵巢功能不全研究24项.注册地域涉及8个省级行政区,其中注册项数最多的是北京市和广东省,共占43.9％(18/41).经费资助来源主要为地方财政.注册试验以干预性研究为主(38/41,92.7％),研究设计以随机平行对照试验为主(32/41,78.0％).干预措施多为单纯针刺和中成药,结局指标以疗效指标为主,方法学质量有待提升.结论 中医药治疗卵巢功能障碍临床试验注册数量呈逐年上升趋势,但整体数量仍偏少.目前研究在注册细节和研究方案方面仍需优化,注册质量有待提升.

目的:分析现代针灸临床文献治疗子宫肌瘤的取穴规律及特点.方法:检索自建库起至2021年4月30日中国期刊全文数据库、万方数据库、VIP、PubMed、Embase以及Cochrane发表的针灸治疗子宫肌瘤临床文献,收集腧穴处方信息并利用中医传承辅助平台(TCMISS V2.5)进行腧穴的描述性分析、关联规则及熵聚类分析.结果:本研究共提取101首针灸处方,腧穴总使用频次为675次,累积样本量5 957例,主要归经为任脉、脾经与胃经,以阴经取穴为主;腧穴使用频次多与肝脾胃相关,注重特定穴的应用,如交会穴、募穴,局部取穴与病灶关系密切;最常用的穴对为关元、子宫和三阴交组成的腧穴交叉配伍;获取强关联腧穴组合规则216条,基于熵聚类算法获取10个核心腧穴组合及6首新腧穴处方.结论:本研究发现的取穴规律、核心组合与新方组合为针灸治疗子宫肌瘤的临床治疗带来新的启发.

Taking regulating the thoroughfare vessel and the conception vessel, tonifying liver and kidney, calming mind as the treatment principle, Tiaojing Cuyun acupuncture (acupuncture for regulating menstruation and promoting pregnancy) is commonly used in clinical treatment of diseases with ovarian function decline, and recommends full cycle acupuncture treatment. Clinical research shows that Tiaojing Cuyun acupuncture can improve menstruation and ovulation, increase the reserve function and response of ovary as well as endometrial receptivity, so as to improve the pregnancy outcome. It can also improve the related symptoms caused by negative emotions and low estrogen, and comprehensively enhance the health related quality of life in patients. The mechanism of Tiaojing Cuyun acupuncture mainly involves 2 aspects, i.e. overall regulation on hypothalamus-pituitary-ovary (HPO) axis and the local regulation on FSH/cAMP signal transduction in ovarian granulosa cells.

目的:通过数据挖掘技术分析现代针灸文献治疗早发性卵巢功能不全(POI)的取穴选用规律.方法:检索自建库至2021年3月31日中国知识基础设施数据库(CNKI)、中国学术期刊数据库(CSPD)、中文科技期刊数据库(CCD)、PubMed、Cochrane Library以及Embase收录的针刺治疗POI的相关文献,收集处方中的穴位信息,应用中医传承辅助系统(V2.5)进行数据挖掘.结果:共纳入文献78篇,腧穴64个,累积使用频次478次,主要归经为膀胱经、任脉、脾经等;针刺治疗常用腧穴为关元、三阴交、肾俞、子宫、足三里、太冲、中极、血海、脾俞、次髎等;注重特定穴的应用,如交会穴、募穴;常选用的腧穴组合为关元-三阴交-肾俞;基于熵聚类算法获取候选新腧穴组合9个及新腧穴处方2首.结论:针刺治疗POI取穴经脉主要以膀胱经、任脉、脾经为主,以关元-三阴交-肾俞为组方核心腧穴,以调冲任、补肝肾、调气血、安神志为针刺治疗POI方法.

目的:探讨古代文献中针灸治疗闭经的选穴规律.方法:利用《中华医典》第5版数据库检索"不月""月事不来""闭经""血枯"等30个关键词,筛选并提取针灸治疗闭经的处方条文,对数据进行整理.利用文献计量学的方法,借助古今医案云平台(V2.3)对针灸治疗闭经的选穴方案进行频次统计、社团分析、关联分析和复杂网络分析.结果:共筛选出符合要求的202条穴位处方条文,其中27条为复方,余为单穴方,共涉及38个腧穴,268频次.治疗闭经使用频次排前15位的腧穴:中极、血海、水泉、阴交、气海、关元、照海、带脉、腰俞、中髎、气冲、太冲、三阴交、合谷、会阴.复杂网络分析显示核心处方为:足三里、三阴交、肾俞、中极、血海、合谷、照海、气冲、气海、阴交.结论:针灸治疗闭经的取穴具有规律性,本研究结果可为临床提供一定的参考性,且通过数据挖掘技术探讨古籍针灸文献具有可行性.

The application status of acupuncture and moxibustion therapy for assisted reproductive field in the United States was analyzed, and the existing problems and future development directions were discussed. According to the survey on the 456 websites of assisted reproductive clinic in the United States mentioned in the report of U.S. Centers for Disease Control and Prevention (CDC), 111 clinics among 456 assisted reproductive clinics recommend and used acupuncture and moxibustion therapy, accounting for 24.3%. Acupuncture and moxibustion therapy had obvious effect, good safety and low cost, and the assisted reproductive institutions in the United States had a high degree of application and recognition to acupuncture and moxibustion therapy. However, some problems, such as immature treatment scheme, unclear mechanism and imperfect insurance policies, still existed. In the future, the advantages of Chinese traditional acupuncture and moxibustion should combine with international modern assisted reproductive technology, and multi-center and large-sample clinical randomized controlled trials and basic experimental research on the mechanism of acupuncture and moxibustion for assisted reproduction should be carried out.

妊娠期针灸的安全性自古以来颇受争议,2019年9月20日英国妇产科杂志(BJOG)发表的"妊娠期针灸的安全性:一项回顾性队列研究"一文的研究结论为针灸是一种无不良反应的安全治疗方法.该研究是第一个国家型的大型队列研究,其结论将令国际医学界对孕期针灸更多一分信任.作者主要结合针灸相关理论和妊娠期安全性相关标准并综合目前的研究成果,就结局指标、暴露因素以及"妊娠禁穴"等方面展开探讨,以期为妊娠期针灸的安全性研究提供建议与思考.

目的:构建针灸对早发性卵巢功能不全(POI)患者妊娠结局影响的列线图临床预测模型,为针灸改善POI患者妊娠结局提供预测方法.方法:从国际针灸病例注册登记研究平台(IPRPAM)导出真实世界POI患者针灸诊疗数据,采用最小绝对收缩选择算子(LASSO)回归法筛选对接受针灸治疗POI患者妊娠结局的影响较大的因素;以LASSO回归筛选的因素为自变量,随访时间和妊娠结局为时间相关的因变量,构建多因素Cox比例风险回归模型,以列线图可视化方式呈现临床预测模型.结果:共纳入90例POI患者,年龄、受教育程度、既往流产史、基础FSH水平、基础AFC、针灸频次6个变量为影响POI患者针灸后是否妊娠的影响因素,calibration校正曲线计算该预测模型具有较好的准确性和校准度.结论:LASSO-Cox模型构建针灸对POI患者妊娠结局影响的临床预测模型具有较好的临床应用价值.

It has been confirmed by growing evidence that common hormone replacement therapy is associated with an increasing risk of causing cardiovascular disease and cancer, while complementary and alternative medicine (CAM) is gaining popularity and application in more and more patients with premature ovarian failure (POF). Although there is little data concerning the clinical safety and efficacy of CAM, the literature includes application studies on the phytoestrogen-rich herbal, acupuncture treatment and intervention therapy. This article reviews recent literature on CAM therapy for POF, aiming to provide theoretical support for clinical application.

To explore the basic principles and methods of quality control of clinical registry research in the field of acupuncture. This study drawed on the data quality control methods of clinical trials in the United States and combined clinical practice experience, based on the "International Patient Registry Platform of Acupuncture and Moxibustion", and the registry study of acupuncture treatment for early-onset ovarian insufficiency as a model. The principles of accuracy, authenticity, consistency and completeness were followed. A remote and on-site quality control method with remote quality control as the main and on-site quality control as the supplement is formed, with a view to providing ideas and reference for the quality control of registry research.