Proton-coupled electron transfer (PCET) at the solid-liquid interface is crucial for addressing the efficiency reduction of zerovalent iron (ZVI) materials in environmental pollution control, which stems from hindered iron cycling. In this study, we report a tannic acid-modified ZVI (TA-ZVI) that accelerates PCET by engineering an interfacial hydrogen-bonding network at the outer Helmholtz plane (OHP). Grafting ortho-phenolic hydroxyl groups onto ZVI reconstructs the interfacial water network, increases the fraction of weakly bound/free water, and enhances interfacial solvation/polarization relaxation, thereby lowering the kinetic barrier for PCET and promoting the generation and utilization of H*. The H* is subsequently delivered through the interfacial hydrogen-bonding network via a thermodynamically favorable Grotthuss-like pathway to the Fe(III) sites, thereby accelerating the iron cycle and enhancing the activity of the surface-bound Fe(II)-mediated 2e- ORR-Fenton reaction. Mechanistic investigations using phenolic analogues identify that ortho-phenolic hydroxyl groups are uniquely effective relative to para-/meta-configurations, owing to the formation of bidentate hydrogen bonds that confine and stabilize H*. Using sulfamethazine (SMT) as a model contaminant, TA-ZVI achieves 85% removal, substantially outperforming pristine ZVI, which removes only 19.8%. TA-ZVI also maintains effective and continuous SMT removal in real wastewater matrices and sustains stable operation for 1000 min in a continuous-flow membrane reactor. This work establishes dynamic hydrogen-bond-network engineering as a molecular strategy for regulating interfacial PCET and enhancing ZVI-based oxidative remediation.

Micron zero-valent iron (mZVI) has been widely employed for heavy metal remediation. However, its surface oxide layer severely restricts interfacial electron transfer, resulting in low decontamination efficiency. Herein, a tannic acid-modified mZVI (TA-ZVI) was developed using ball milling. The modified material exhibited excellent Cr(VI) removal efficiency (98.2 % in 2 min) due to the abundant presence of surface hydroxyl groups. The results of electrochemical analysis demonstrated that the hydroxyl groups increased the electron density and conductivity of the material surface, accelerated the electron transfer, and led to the formation of low and uniform surface potential on the material surface. The hydroxylated interface facilitated in situ Fe(II) generation and release to the solution. The hydroxyl groups then complexed with Fe(II) in the solution to form =FeOFe(II)+, which facilitated protonic reactions and led to the formation of =FeOH2+. Density functional theory (DFT) calculation proved that the reaction energy barrier of =FeOFe(II)+ for Cr(VI) was much smaller than that of Fe(II) in the solution. Moreover, the surface hydroxyl species (=FeOH and =FeOH2+) directly participated in Cr(VI) removal through synergistic adsorption-reduction pathways, thereby contributing significantly to the overall removal. This work reveals the removal mechanism of Cr(VI) by surface-hydroxylated ZVI, which is of great significance for the treatment of heavy metal ion pollution.

The high-performance electrochemical detection relies on sensing material with highly active structure, which requires the development of advanced synthesis technique and the study of its structure-activity mechanism. Herein, the few-layer Ti3C2Tx 3 C 2 T x nanosheets densely coated zeolitic imidazole framework-8 nanoparticles (Ti3C2Tx@ZIF-8) 3 C 2 T x @ZIF-8) have been successfully prepared as a unique precursor, which combines the structural advantages of two-dimensional Ti3C2Tx 3 C 2 T x and porous ZIF-8 nanoparticles, leading to the vast interior spaces for loading electrocatalytic nanomaterials. For this purpose, Ti3C2Tx@Au 3 C 2 T x @Au nanoparticles-ZnO nanoparticles@N-doped carbon (Ti3C2Tx@AuNPs-ZnO@NC) 3 C 2 T x @AuNPs-ZnO@NC) has been obtained for the simultaneous electrochemical detection of pharmaceutical molecules including dopamine (DA), acetaminophen (AC), and xanthine (XA). The materials characterization and sensing analysis results of Ti3C2Tx@AuNPs-ZnO@NC 3 C 2 T x @AuNPs-ZnO@NC reveal the structure-activity relationship of Ti3C2Tx, 3 C 2 T x , AuNPs and NC resulting in the high-performance behaviors, which can be summed up as stable electrochemical active sites and efficient electron transport channels. First, the abundant anchor points are offered by NC for immobilizing AuNPs, which ensure the electrochemical activity of such material. Second, the close combination of few-layer Ti3C2Tx 3 C 2 T x nanosheets and NC provides an ideal channel for electron transmission along with the electrochemical reaction process. The potential application of Ti3C2Tx@AuNPs-ZnO@NC 3 C 2 T x @AuNPs-ZnO@NC is displayed to develop the electrochemical medical sensor. The detection limits are 41 nM towards DA, 59 nM towards AC, and 67 nM towards XA. The linear ranges are 3-200 mu M for DA, 15-500 mu M for AC, and 8-350 mu M for XA.

Most personal protective equipment is absent of adequate antibacterial and antiviral activity and sufficient storage stability. This limits the effectiveness in preventing the spread and infection of emerging infectious diseases (EIDs). In this study, we report a multifunctional helical encapsulated nanofiber composite membrane (HENM) with ultralong photoactive storage capability as well as rechargeable antibacterial and antiviral activities. HENM rapidly releases reactive oxygen species (ROS) under both light and dark conditions and can be stored for a longer period. This ensures the pharmacological stability of the photoactive agent and thus provides an "ultralong standby" killing function. The resultant HENM demonstrated long-term photoactive storage (>5 months), stable ROS release, high encapsulation efficiency (95.62%), and rapid charging rate (center dot OH 288.35 and H2O2 8.86 mu g/(gmin)). Moreover, its particle interception and antibacterial and antiviral efficacy reach >99.99%, >99.99%, and >99.97% respectively. This HENM enables the function as a scalable biocidal layer for protective equipment, facilitating contact killing of pathogens in both aerosol and liquid forms. The successful synthesis of these fascinating HENMs provides ideas for developing sustainable protective materials with ultralong light-active storage, air filtration, self-charging capability, and adaptive forms.

Herein, an iodine-promoted reductive sulfenylation reaction of ketones with disulfides has been developed. This method provides an approach for synthesizing unsymmetrical alkyl-alkyl and alkyl-aryl sulfides in a single step. Investigation of the reaction mechanism revealed that ketones play a dual role in this process. They react with disulfides to produce vinyl thioethers and act as effective organic hydride donors, reducing the number of vinyl thioethers that are formed in situ. This study expands the range of applications of ketones in chemical synthesis.

Tumorigenesis and metastasis are highly dependent on the interactions between the tumor and the surrounding microenvironment. In 3D matrix, the fibrous structure of the extracellular matrix (ECM) undergoes dynamic remodeling during tumor progression. In particular, during the late stage of tumor development, the fibers become more aggregated and oriented. However, it remains unclear how cancer cells respond to the organizational change of ECM fibers and exhibit distinct morphology and behavior. Here, we used electrospinning technology to fabricate biomimetic ECM with distinct fiber arrangements, which mimic the structural characteristics of normal or tumor tissues and found that aligned and oriented nanofibers induce cytoskeletal rearrangement to promote directed migration of cancer cells. Mechanistically, caveolin-1(Cav-1)-expressing cancer cells grown on aligned fibers exhibit increased integrin β1 internalization and actin polymerization, which promoted stress fiber formation, focal adhesion dynamics and YAP activity, thereby accelerating the directional cell migration. In general, the linear fibrous structure of the ECM provides convenient tracks on which tumor cells can invade and migrate. Moreover, histological data from both mice and patients with tumors indicates that tumor tissue exhibits a greater abundance of isotropic ECM fibers compared to normal tissue. And Cav-1 downregulation can suppress cancer cells muscle invasion through the inhibition of YAP-dependent mechanotransduction. Taken together, our findings revealed the Cav-1 is indispensable for the cellular response to topological change of ECM, and that the Cav-1/YAP axis is an attractive target for inhibiting cancer cell directional migration which induced by linearization of ECM fibers.

Combining photodynamic antimicrobials with nonwovens is prospective. However, common photosensitizers still have drawbacks such as poor photoactivity and the inability to charge. In this study, a photodynamic and high-efficiency antimicrobial protective material was prepared by grafting bis benzophenone-structured 4,4-terephthaloyl diphthalic anhydride (TDPA) photosensitizer, and antimicrobial agent chlorogenic acid (CA) onto spunbond-meltblown-spunbond (SMS) membranes. The charging rates for center dot OH and H2O2 were 6377.89 and 913.52 mu g/g/h. The light absorption transients structural storage remained above 69% for 1 month. High electrical capacity remained after seven cycles indicating its rechargeability and recyclability. The SMS/TDPA/CA membrane has excellent bactericidal performance when under illumination or lightless conditions, and the bactericidal efficiency of Escherichia coli and Staphylococcus aureus reached over 99%. The construction of self-disinfection textiles based on the photodynamic strategies proposed in this paper is constructive for expanding and promoting the application of textile materials in the medical field. The graphical abstract is intended to show that this study enabled SMS nonwovens to be successfully loaded with 4,4-terephthaloyl diphthalic anhydride and chlorogenic acid through two immersions, which led to the production of reactive oxygen species (ROS) and the killing of bacteria under light conditions. The section at the bottom left of the image is the cyclic generation mechanism of ROS.image

This study designed a novel co-electrospun cellulose acetate (CA)/thermoplastic polyurethane (TPU) photodynamic helical fiber antibacterial membrane as a potential environmentally friendly medical protective material. A central combined design method (CCD) based on response surface methodology (RSM) was used to analyze essential variables' influence. The optimized parameters for CCD were TPU (wt%) 11.68 %, CA (wt%) 13.89 %, DMAc/ACE volume ratio 0.147, LiCl (wt%) 1.39 %, and voltage (kV) 14.43 V. Pitch and pitch diameter were the response process as the critical output variable. The membranes were characterized by SEM, TG, FT-IR, and molecular structure analysis. The results showed that the photodynamic helical fiber antimicrobial membrane exhibited synergistic effects of the antibacterial photodynamic therapy (APDT) and antimicrobial agent under average daylight irradiation. The release rate of-OH was 98.22 %, and H2O2 was 88.36 % under the action of 20 min of light. The bactericidal rates of S. aureus and E. coli reached 99.9 % and 99.7 %, respectively. The fiber helical structure can increase the light absorption rate, thus increasing the release rate and amount of reactive oxygen species (ROS) species, increasing the antibacterial rate. After washing five times, the antibacterial membrane has excellent antibacterial performance and a dark antibacterial effect.

Antimicrobial photodynamic therapy (APDT) encapsulation technology, as a novel antimicrobial technology, achieves efficient and steady killing of bacteria by encapsulating photosensitizers (PSs) in different carrier structures. However, recent studies have shown the encapsulation efficiency, material selection, encapsulation pathway limitations, and complexity in encapsulated PSs, which have forced the development of APDT encapsulation technology to face the challenges posed by PS encapsulation issues. In this paper, based on the analysis of utilizing encapsulated PSs in different carrier structures to achieve efficient bacterial killing, various approaches and recent advances in APDT encapsulation technology are reviewed. First, the selection of varying encapsulation materials and diverse encapsulation methods to utilize their advantages in APDT therapy are described. Then, performance evaluation and application after encapsulation are discussed to ensure the safety and efficacy of the application. Finally, the future research and development trends of APDT encapsulation technology are envisioned. This review aims to provide timely and essential guidance for developing APDT encapsulation technology.

OBJECTIVE To investigate the causes of ineffectiveness of platelet transfusion with monoclonal antibody solid phase platelet antibody test (MASPAT) matching in patients with allogeneic hematopoietic stem cell transplantation and explore the strategies of platelet transfusion. METHODS A case of donor-specific HLA antibodies (DSA) induced by transfusion which ultimately resulted in transplantation failure and ineffective platelet transfusion with MASPAT matching was selected, and the causes of ineffective platelet transfusion and platelet transfusion strategy were retrospectively analyzed. RESULTS The 32-year-old female patient was diagnosed as acute myeloid leukemia (high risk) in another hospital with the main symptoms of fever and leukopenia, who should be admitted for hematopoietic stem cell transplantation after remission by chemotherapy. In the course of chemotherapy, DSA was generated due to platelet transfusion, and had HLA gene loci incompatible with the donor of the first transplant, leading to the failure of the first transplant. The patient received platelet transfusion for several times before and after transplantation, and the results showed that the effective rate of MASPAT matched platelet transfusion was only 35.3%. Further analysis showed that the reason for the ineffective platelet transfusion was due to the missed detection of antibodies by MASPAT method. During the second hematopoietic stem cell transplantation, the DSA-negative donor was selected, and the matching platelets but ineffective transfusion during the primary transplantation were avoided. Finally, the patient was successfully transplanted and discharged from hospital. CONCLUSIONS DSA can cause graft failure or render the graft ineffective. For the platelet transfusion of patients with DSA, the platelet transfusion strategy with matching type only using MASPAT method will miss the detection of antibodies, resulting in invalid platelet transfusion.

The effects of protein oxidation on the emulsion gel properties of myofibrillar protein (MP) in the presence of tetrasodium pyrophosphate (TSPP) and soybean protein isolate (SPI) were investigated from the perspective of interfacial protein interactions. The results showed that the emulsifying activity and emulsion stability of MP increased by 35.2 %-181.6 % with elevated H2O2 concentrations (1-20 mM), while the gel strength and water holding capacity of MP emulsions first increased to a maximum at 5 mM H2O2 and then decreased. TSPP and SPI further reinforced the effects caused by oxidation. The emulsifying properties of MP and its emulsion gel properties were closely related to surface hydrophobicity/hydrogen bonds/hydrophobic interactions and disulfide bonds among interfacial proteins, respectively. However, these correlations became difficult to define when TSPP and SPI were introduced. The study provides a theoretical basis for the strategy development to reduce protein oxidation damage on meat product quality.

Purpose: V-domain immunoglobulin suppressor of T-cell activation (VISTA) is a novel type of immune check-point. This study was performed to explore the potential mechanism by which different domains of VISTA affect T-cell activation and search for potential interacting proteins.Methods: Stably transfected Jurkat cell lines were constructed to overexpress human VISTA (VISTA-FL), cyto-plasmic domain deletion mutants (VISTA-AECD) and extracellular domain deletion mutants (VISTA-ACD). Empty vector (EV) control cell lines were constructed. Four stable cell lines were subjected to transcriptome sequencing after stimulation with PMA and PHA. The differentially expressed genes (DEGs) were analysed to explore the potential pathway by which VISTA inhibits T-cell activation. Protein-protein interaction (PPI) network analysis was used to search for potential interacting proteins of VISTA.Results: In this study, 1256 DEGs were identified in Jurkat-VISTA-FL cells, 740 DEGs in Jurkat-VISTA-ACD cells, and 5605 DEGs in Jurkat-VISTA-AECD cells compared with Jurkat-EV cells. DEGs were mainly enriched in pathways related to T-cell differentiation, T-cell receptor signalling pathway and T-cell migration in Jurkat-VISTA-AECD cells; with cholesterol biosynthesis in Jurkat-VISTA-ACD cells; and with the inflammatory response in Jurkat-VISTA-FL cells. HHLA2 and CTH were identified as potential partners that interact directly with VISTA. The results also show an indirect interaction between VISTA and PSGL-1.Conclusions: This study revealed the pathways by which VISTA is involved in T-cell activation and identified the potential binding partners of VISTA through RNA-seq, providing valuable resources for developing in-depth studies of the action mechanisms of VISTA as a potential target for cancer and inflammatory diseases.

Drug-resistant pathogens pose significant pressures and challenges for medical protective materials with highly effective antibacterial/antiviral and rechargeable properties. In this study, a clean, rechargeable photodynamic antibacterial/antiviral strategy was proposed after nanofibrous membranes were grafted by photosensitive 4,4,-terephthalic di-phthalic anhydride (TDPA) and natural polyphenol chlorogenic acid (CA) antibacterial agent. The resultant membrane released the maximum 'OH and H2O2 capacities of 6188.56 lg/g and 842.00 lg/g, respectively, exhibited the storage antibacterial stability even stored for 30 days in the dark. After seven quenching, the charging capacity retained more than 70% of the orig-inal. The antibacterial efficiency of the membrane showed more than 99%. In addition, the antibacterial efficiency decreased by only 3% after water washing. Moreover, it demonstrated an excellent antiviral property whose antiviral activity against H3N2 achieved 3.6 PFU/mL. This daylight-driven rechargeable nanofibrous membrane can be used to develop reusable medical protective materials with rechargeable antibacterial and antiviral efficiency.CO 2023 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

Due to recent serious COVID-19 pandemic, medical protective materials with outstanding microbial barrier and liquid barrier property have attracted much attention. However, the crucial issue of improving the waterproof-breathable capability and degradable properties of medical protective material is still challenging. In this research, poly(butyleneadipate-co-terephthalate) (PBAT)/hemp@silica-polyvinylsilsesquioxane (SiO2-PVSQ) composite membranes with good moisture permeability and waterproof property were fabricated. Firstly, hemp and cotton fibers were mixed and hemp/cotton hydroentangled nonwovens were produced to endow the membranes with outstanding water vapor penetration and mechanical properties. Then, degradable PBAT macroporous membranes were prepared by non-solvent induced phase separation (NIPS) method, and combined with hemp/cotton hydroentangled nonwovens to endow the composite membranes with waterproof property. Finally, SiO2 particles were hydrophobically modified by vinyltrimethoxysilane (VTMOS) and grafted on the surface of PBAT/hemp composite membranes. The waterproof property of composite membranes was further improved by hierarchical rough structure and low surface tension hydrophobic groups on the membrane surface. The prepared PBAT/hemp@SiO2-PVSQ composite membranes showed excellent tensile strength with high moisture permeability, good waterproof and water contact angle of 139.5°, indicating promising candidates for the new generation comfort and degradable medical protective textiles.

Bacterial infection is one of the major threats to human health worldwide, posing a great challenge to the development of medical protective materials with efficient filtration and long-lasting antimicrobial properties. In this study, electrospinning technology and non-woven fabrics were combined to construct a micro/nano-structured photodynamic rechargeable and storable antibacterial filtration composite membrane. The filtration efficiency of the composite membrane is as high as 99.99% for 0.5-5 lm particles, and the resistance pressure drop is 85 Pa. In addition, the release of center dot OH and H2O2 from the composite film reached 5326.16 lg/g and 711.93 lg/g, respectively. After 7 cycles of quenching, the charging capacity retains more than 70 % of the original. It showed good antimicrobial properties even when stored under dark conditions for one month. The bactericidal efficiency of the composite membrane against E. coli and S. aureus exceeded 99% under both dark and light conditions. The development of this simple and clean micro/nanofiber membrane provides a new idea for exploring photoactive antimicrobial and filtration materials for medical protection. (c) 2023 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.

Photodynamic antibacterial composite membranes were engineered by integrating photosensitizer/antibacterial agent, into the polyurethane adhesive blend and coating the blend on co-electrospinning cellulose acetate(CA)/thermoplastic polyurethanes(TPU) composite membrane support for enhanced antibacterial performance. The central composite design (CCD) method based on the response surface method (RSM) was used for analysis to illustrate the influence of important variables. The optimized parameters of CCD were TPU (wt%) 22.64%, CA (wt%) 18.63, DMAC/acetone volume ratio 0.85, LiCl (wt%) 0.95%, voltage (kV) 23.33 kV. Fiber diameter was the key response process output variable. The membranes were characterized by SEM, XPS, and molecular structure analyses. The model had excellent applicability as a tool to realize the average diameter of the CA/TPU bicomponent electrospun membrane. Results showed that after coating, the synergistic effects of photodynamic antibacterial and antibacterial agent performance on the Antibacterial composite membrane were achieved under ordinary daylight irradiation. The sterilization rate of S. aureus and E. coli could achieve excellence at 99.2% and 93.4%, respectively. This method of preparing photodynamic antibacterial composite film provides a new direction for the design of medical antibacterial protective materials.

OBJECTIVE:To establish a new method for synthesizing Lewis blood group antigens, that is, the mimotopes of Lewis blood group antigens were screened by using an alpaca phage display nanobody library.METHODS:We selected mimotopes of the Lewis a (lea) antigen by affinity panning of an alpaca phage display nanobody library using a monoclonal anti-lea antibody. Enzyme-linked immunosorbent assay (ELISA) was used to test the affinity of the positive clones for the monoclonal anti-lea antibody, and the high-affinity positive clones were selected for sequencing and synthesis. Finally, the sensitivity, specificity and reactivity of the synthesized lea mimotope in clinical samples were verified by ELISA.RESULTS:A total of 96 phage clones were randomly selected, and 24 were positive. Fourteen positive clones with the highest affinity were selected for sequencing. The result showed that there were 5 different sequences, among which 3 sequences with the highest frequency, largest difference and highest affinity were selected for expression and synthesis. The sensitivity and specificity of lea mimic antigen by ELISA showed that, the minimum detection limit of gel microcolumn assay (GMA) and ELISA method were 25 times different, and the lea mimic antigen had no cross reacted with the other five unrelated monoclonal antibodies(P<0.001). Finally, 30 clinical plasma samples were analyzed. The mean absorbance of the 15 positive plasma samples was significantly higher than that of the 15 negative plasma samples (P=0.02). However, the positive signal values of the clinical samples were much lower than those of the monoclonal antibodies.CONCLUSION:A new method of screening lea mimic antigen by using alpaca phage nanoantibody library has been established, which is expected to realize the screening of lea mimotopes, thus realizing the application of high-sensitivity detection methods such as ELISA and chemiluminescence in blood group antibody identification.

OBJECTIVE:A dynamic gel loaded with lyophilized platelet-rich plasma-chitosan/difunctionalized polyethylene glycol (LPRP-CP) was prepared to investigate its hemostatic antibacterial and promoting wound healing of scald wounds through in vitro and in vivo experiments. METHODS:In this study, normal gauze/blank tablet (Ctrl), LPRP-CP, Chitosan HUCHUANG Powder(Chito P)and ChitoGauze XP PRO group (Chito G group) were set. The hemostatic effect and promoting healing effect of the four groups of materials were evaluated by establishing rabbit ear artery hemorrhage model and superficial Ⅱ° scalded model of skin on the back. The hemostatic time and bleeding amount were calculated and the gross and histological results of scald healing were observed. The antibacterial effect of the four groups of materials was evaluated by antibacterial test in vitro. RESULTS:In the rabbit ear arterial hemorrhage model, the hemostasis of all materials was successful. The hemostatic time of Ctrl, Chito P, LPRP-CP and Chito G groups was 213.33±38.30, 118.33±24.01, 115.00±8.37 and 111.67±11.69 s, respectively. The blood loss was 1233.83±992.27, 346.67±176.00, 193.33±121.47 and 147.50±80.66 mg, respectively. Compared with Ctrl, the hemostasis time of LPRP-CP, Chito P and Chito G group was significantly shorter (P<0.001), and the amount of blood loss of LPRP-CP and Chito G group was decreased (P<0.05). Compared with LPRP-CP, there were no significant differences in hemostatic time and blood loss between Chito P and Chito G group (P>0.05). In the model of superficial Ⅱ° scalded on the back of rabbit, the wound healing rate of LPRP-CP was faster than that of the other three groups at the same time, and the healing effect was perfect. In the antibacterial test in vitro, only LPRP-CP had better anti-S. aureus effect, and all groups had no anti-E. coli effect. CONCLUSION:LPRP-CP is an excellent hemostatic material for superficial wounds, and has certain antibacterial and wound healing effects, which has a wide academic value and research prospects.

The research on the processing mechanism of Chinese medicine is the key and core foundation to improve processing technologies of Chinese medicine, formulate the quality standards of Chinese medicinal pieces, enhance the clinical efficacy of Chinese medicine, enrich Chinese medicine processing theories, and promote the development of Chinese medicine processing. Many researc-hers have conducted in-depth exploration on the processing mechanism of Chinese medicine in the 20 years in the 21 st century. Significant progress has been made in the transformation of chemical components during the processing, the change of active components in the body, the law of toxicity attenuation in the processing of toxic Chinese medicine, the mechanism of efficacy enhancement and toxicity attenuation of processing with auxiliary materials, and the application of new biomedical technologies. At present, the processing mechanism of multiple Chinese medicines has been preliminarily clarified, which has greatly promoted the development of Chinese me-dicine processing. The development of the processing mechanism of Chinese medicine reveals that the in vitro transformation of chemical components is combined with the in vivo absorption, transport, and metabolism, and the macroscopic biological effects of the organism are combined with the cells, molecules, targets, and pathways in the study of the processing mechanism of Chinese medicine. More attention has been paid to exploring the processing mechanism from the overall level, and a modern systematic research system on the processing mechanism of Chinese medicine has been initially formed. To further promote the scientific development of Chinese me-dicine processing, the present study proposed that the research on the processing mechanism of Chinese medicine should take Chinese medicine properties into account, focus on the influence of disease condition on the mode of action and effect strength of the drugs, comply with the characteristics of clinical compound compatibility of Chinese medicine, use the holistic view research strategies of systems bio-logy, and deeply explore the processing mechanism of Chinese medicine from traditional Chinese medicine theories and the characteristics of clinical medication of Chinese medicine.