Aquaculture industry represents a continuously growing sector playing a fundamental role in pursuing United Nation's goals. Increasing sea-surface temperatures, the growth of encrusting species and current cage cleaning practices proved to affect the productivity of commercial species. Here, through a Dynamic Energy Budget application under two different IPCC scenarios, we investigate the long-term effects of Pennaria disticha fragments' on Mytilus galloprovincialis' functional traits as a result of cage cleaning practices. While Climate-Change did not exert a marked effect on mussels' Life-History traits, the simulated effect of cage cleanings highlighted a positive effect on total weight, fecundity and time to commercial size. West-Mediterranean emerged as the most affected sector, with Malta, Montenegro, Morocco, Syria, Tunisia and Turkey between the top-affected countries. These outcomes confirm the reliability of a DEB-approach in projecting at different spatial and temporal scale eco-physiological results, avoiding the limitation of short-term studies and the difficulties of long-term ones.

The Turkish aquaculture sector is growing very rapidly, yet the impacts of the pandemic on both sea and inland water operations remain unclear. To provide a knowledge baseline based on stakeholders, we carried out a rapid assessment in the present study. The primary objectives were to evaluate the stakeholder perceptions regarding economic loss attributed to the COVID-19 pandemic, the effectiveness of implemented mitigating measures, and the overall impact on health and wellbeing. The study also asked respondents about the disruption and loss they felt as a result of the consequences of anthropogenic stresses over the previous ten years. Out of 195 survey participants, only 107 completed and were assessable (73 from land-based farms and 34 from sea-based farms). Ninety percent of those surveyed said they had suffered various levels of financial loss as a result of the COVID-19 epidemic, especially in land-based systems. Loss of market and clients appeared to be a major issue, particularly for land-based farmers. To deal with the problem, sea-based farmers tried to develop integrated aquaculture systems, change farming systems and increase the link with scientists, whereas land-based operations preferred changing marketing methods such as direct sales to consumers, substitute market and processing methods to deal with the problem. The respondents reported higher loss associated with anthropogenic stressors, namely diseases and heat waves. Our results suggest that the decision-makers should be more prepared for such unexpected crises and take anthropogenic stressors into account when designing future recovery strategies for this productive sector.

DEBEcoMod is an open-source R script designed to apply Dynamic Energy Budget (DEB) theory to predict life-history traits of marine organisms under various environmental and anthropogenic stressors. It presents a novel approach to overcoming the computational and scale limitations of previous DEB applications, enabling the generation of spatially explicit outputs. DEBEcoMod is intended to predict traits such as maximum size, reproductive output, and life-history traits across different temporal and spatial scales. It utilises parameters from the AddMyPet database for various species and environmental time series to simulate the past, present, and future performance of organisms. The tool also includes a module for spatio-temporal representation, producing clear and accessible maps for stakeholders. The document highlights DEBEcoMod's application in invasion biology, marine spatial planning, integrated multi-trophic aquaculture, and marine ecology, drawing on published examples of spatial applications to demonstrate its versatility and potential in ecological research and adaptive management. Furthermore, the code has been cross-validated with the official DEBtool to ensure its accuracy and reliability. DEBEcoMod is available for download on GitHub, enhancing its accessibility and utility for a wide range of ecological and conservation applications.

Altered ocean chemistry caused by ocean acidification (OA) is expected to have negative repercussions at different levels of the ecological hierarchy, starting from the individual and scaling up to the community and ultimately to the ecosystem level. Understanding the effects of OA on benthic organisms is of primary importance given their relevant ecological role in maintaining marine ecosystem functioning. The use of functional traits represents an effective technique to investigate how species adapt to altered environmental conditions and can be used to predict changes in the resilience of communities faced with stresses associated with climate change. Artificial supports were deployed for 1-y along a natural pH gradient in the shallow hydrothermal systems of the Bottaro crater near Panarea (Aeolian Archipelago, southern Tyrrhenian Sea), to explore changes in functional traits and metabolic rates of benthic communities and the repercussions in terms of functional diversity. Changes in community composition due to OA were accompanied by modifications in functional diversity. Altered conditions led to higher oxygen consumption in the acidified site and the selection of species with the functional traits needed to withstand OA. Calcification rate and reproduction were found to be the traits most affected by pH variations. A reduction in a community’s functional evenness could potentially reduce its resilience to further environmental or anthropogenic stressors. These findings highlight the ability of the ecosystem to respond to climate change and provide insights into the modifications that can be expected given the predicted future pCO2 scenarios. Understanding the impact of climate change on functional diversity and thus on community functioning and stability is crucial if we are to predict changes in ecosystem vulnerability, especially in a context where OA occurs in combination with other environmental changes and anthropogenic stressors.

Microplastics represent an ever-increasing threat to aquatic organisms. We merged data from two global scale meta-analyses investigating the effect of microplastics on benthic organisms' and fishes' functional traits. Results were compared, allowing differences related to vertebrate and invertebrate habitat, life stage, trophic level, and experimental design to be explored. Functional traits of aquatic organisms were negatively affected. Metabolism, growth, and reproduction of benthic organisms were impacted, and fish behaviour was significantly affected. Responses differed by trophic level, suggesting negative effects on trophic interactions and energy transfer through the trophic web. The experimental design was found to have the most significant impact on results. As microplastics impact an organism's performance, this causes indirect repercussions further up the ecological hierarchy on the ecosystem's stability and functioning, and its associated goods and services are at risk. Standardized methods to generate salient targets and indicators are urgently needed to better inform policy makers and guide mitigation plans.

The COVID-19 global pandemic has had severe, unpredictable and synchronous impacts on all levels of perishable food supply chains (PFSC), across multiple sectors and spatial scales. Aquaculture plays a vital and rapidly expanding role in food security, in some cases overtaking wild caught fisheries in the production of high-quality animal protein in this PFSC. We performed a rapid global assessment to evaluate the effects of the COVID-19 pandemic and related emerging control measures on the aquaculture supply chain. Socio-economic effects of the pandemic were analysed by surveying the perceptions of stakeholders, who were asked to describe potential supply-side disruption, vulnerabilities and resilience patterns along the production pipeline with four main supply chain components: a) hatchery, b) production/processing, c) distribution/logistics and d) market. We also assessed different farming strategies, comparing land- vs. sea-based systems; extensive vs. intensive methods; and with and without integrated multi-trophic aquaculture, IMTA. In addition to evaluating levels and sources of economic distress, interviewees were asked to identify mitigation solutions adopted at local / internal (i.e., farm-site) scales, and to express their preference on national / external scale mitigation measures among a set of a priori options. Survey responses identified the potential causes of disruption, ripple effects, sources of food insecurity, and socio-economic conflicts. They also pointed to various levels of mitigation strategies. The collated evidence represents a first baseline useful to address future disaster-driven responses, to reinforce the resilience of the sector and to facilitate the design reconstruction plans and mitigation measures, such as financial aid strategies.

The rapid, global spread of COVID-19, and the measures intended to limit or slow its propagation, are having major impacts on diverse sectors of society. Notably, these impacts are occurring in the context of other anthropogenic-driven threats including global climate change. Both anthropogenic stressors and the COVID-19 pandemic represent significant economic challenges to aquaculture systems across the globe, threatening the supply chain of one of the most important sources of animal protein, with potential disproportionate impacts on vulnerable communities. A web survey was conducted in 47 countries in the midst of the COVID-19 pandemic to assess how aquaculture activities have been affected by the pandemic, and to explore how these impacts compare to those from climate change. A positive correlation between the effects of the two categories of drivers was detected, but analysis suggests that the pandemic and the anthropogenic stressors affect different parts of the supply chain. The immediate measurable reported losses varied with aquaculture typology (land vs. marine, and intensive vs. extensive). A comparably lower impact on farmers reporting the use of integrated multitrophic aquaculture (IMTA) methods suggests that IMTA might enhance resilience to multiple stressors by providing different market options under the COVID-19 pandemic. Results emphasize the importance of assessing detrimental effects of COVID-19 under a multiple stressor lens, focusing on areas that have already locally experienced economic loss due to anthropogenic stressors in the last decade. Holistic policies that simultaneously address other ongoing anthropogenic stressors, rather than focusing solely on the acute impacts of COVID-19, are needed to maximize the long-term resilience of the aquaculture sector.

Maerl beds are biogenic benthic habitats distributed worldwide and known to sustain high productivity and biodiversity levels. In the Mediterranean, the number of studies that have been carried out is limited, and little is known on its real distribution, mostly due to the difficulties of exploring such habitats - Due to the high transparency of Mediterranean waters, maerl can be found at depths of over 50 m making mandatory the use of benthic grabs and ROVs. The last published data from Maltese waters were taken two decades ago. In this present study, we provide new insights on this poorly known habitat, in particular regarding the north western bank, designated as a NATURA 2000 protected area, in which human activities are also carried out, such as blue fin tuna ranching. The objective of this work is to provide information regarding this delicate habitat, especially relevant for future management plans and authorities. (c) 2022 The Author(s). Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

Microplastics are widespread in the aquatic environment and thus available for many organisms at different trophic levels. Many scientific papers focus their attention on the study of the effects of microplastics on different species at individual level. Here we performed a global scale meta-analysis focusing our work on the study of the effect of microplastics on the functional traits of aquatic benthic organisms. Overall, microplastics showed a moderate negative effect on the examined functional traits of benthic organisms. Our results show that some crucial functional traits, such as those linked to behaviour and feeding, appear to be unaffected by microplastics. In contrast, traits related to the capacity of organisms to assimilate energy are affected. Moreover, traits with possible effects at population level appear to be negatively affected by microplastics. We discuss how the direct impact of organismal performance may have indirect repercussions at higher levels in the ecological hierarchy and represent a risk for the stability and functioning of the ecosystem.

Aquaculture, the current fastest-growing food sector, is one of the major opportunities that could be reaped to cope with the increased demand for proteins from the sea and simultaneously generate economic growth while ensuring sustainable use of natural resources. The number of tools and approaches suggested to promote the selection of suitable areas - focusing mostly on the management of potential conflicting uses at sea - is rapidly increasing. However, to date, there is a lack of information regarding spatial planning according to a trait-based approach encompassing the functional and biological data of farmed species; a gap that may lead to selecting unsuitable areas for farming. To fill this gap, our study builds on a functional trait-based mechanistic approach based on the Dynamic Energy Budget (DEB) theory allowing to generated species and site-specific predictions of aquaculture performances and the related environmental impact. We applied this approach to a commercial species farmed in the Mediterranean Sea, namely, the European seabass (Dicentrarchus labrax). We used three seeding sizes (1.5, 2, 2.5 g) to run model simulations and answer a crucial question for farm management, i.e. the selection of the best seeding size. A sensitivity analysis coupled with our simulations allowed to spatially represent the performance growth and environmental impact per seeding size across Mediterranean countries. The accuracy of the model's outcome was strengthened by using high-resolution satellite data over a wide area of investigation (c.a. 302,000 km2). The novel informative obtained layers combine both the modelling of aquaculture performance and related environmental impact to fill a lacking perspective within both AZAs (Allocated Zones for Aquaculture) and AZEs (Allowable Zones of Effect) concepts. Our approach allowed discriminating the best zones for European sea bass aquaculture at country level for each of the tested seeding sizes. High-resolution predictions of aquaculture performances and impact were provided for each of the nineteen Mediterranean coastal countries, zooming at Exclusive Economic Zone scale (EEZ). We highlighted pole-ward negative patterns with the best values in the Southern basin; Libya, Tunisia and Egypt in particular. Our spatial contextualization - through high resolution mapped outcomes - represents an effective and salient tool for stakeholders and policy makers, based on the translation of complex computational modelling results into easy-to-read maps. The highlighted patterns may provide scientific evidence for proactive capacity-building programmes at country level in the future.

Around 350 million tonnes of plastics are annually produced worldwide. A remarkable percentage of these products is dispersed in the environment, finally reaching and dispersed in the marine environment. Recent field surveys detected microplastics' concentrations in the Mediterranean Sea. The most commonly polymers found were polyethylene, polypropylene and viscose, ethylene vinyl acetate and polystyrene. In general, the in-situ monitoring of microplastic pollution is difficult and time consuming. The main goals of this work were to spectrally characterize the most commonly polymers and to quantify their spectral separability that may allow to determine optimal band combinations for imaging techniques monitoring. The spectral signatures of microplastics have been analysed in laboratory, both in dry condition and on water surface, using a full spectrum spectroradiometer. The theoretical use of operational satellite images for remote sensing monitoring was investigated by quantifying the spectral separability achievable by their sensors. The WorldView-3 sensor appears the most suitable for the monitoring but better average spectral separability are expected using the recently released PRISMA images. This research was preparatory to further outdoor experiments needed to better simulate the real acquisition condition.

Many recent studies have focused their attention on the physiological stress experienced by marine organisms in measuring ecotoxicological responses. Here we suggest a new approach for investigating the effects of an anthropogenic pollutant on Life-History (LH) traits of marine organisms, to provide stakeholders and policy makers an effective tool to evaluate the best environmental recovery strategies and plans. A Dynamic Energy Budget (DEB), coupled with a biophysical model was used to predict the effects of a six-month oil spill on Mytilus galloprovincialis' LH traits and to test two potential recovery strategies in the central Mediterranean Sea. Oxygen consumption rates were used to check for increasing energetic maintenance costs [ṗM] respectively in oil-polluted system treatments (∼76.2%) and polluted systems with physical (nano-bubbles ∼32.6%) or chemical treatment (dispersant ∼18.4%). Our model outputs highlighted a higher growth reduction of intertidal compared to subtidal populations and contextually an effect on the reproductive output and on the maturation time of this latter. The models also enabled an estimation of the timing of the disturbance affecting both the intertidal and subtidal populations' growth and reproduction. Interestingly, results led to the identification of the chemical dispersant as being the best remediation technique in contexts of oil spill contamination.

The ability to observe and predict trawling-induced patterns at spatial and temporal scales that are relevant to inform realistic management strategies is a challenge which scientists have consistently faced in recent decades. Here, we use fish feeding behaviour, a biological trait easily impaired by trawling disturbance, to depict alterations in fish condition (i.e. individual fitness) and feeding opportunities. The benthivorous fish Mullus barbatus barbatus was selected as a model species. The observed trends of responses to trawling in prey species confirmed the effectiveness of a non-trawled zone in sustaining higher levels of diet diversity (e.g. quantity and quality of ingested prey) and fish condition values (e.g. morphometric and physiological Condition Index). Changes observed in fish prey selection confirmed the role of trawling disturbance in modifying the local soft bottoms community, producing alterations of prey availability that trigger shifts in fish diet. Trawling-induced feeding patterns, mirrored through stomach contents, can positively or negatively affect fish condition, the main driver of population dynamics in maintaining carrying capacity levels. Due to the widespread socio-economic value of the red mullet fishery, and the current exploitation status, evidence gathered by the proposed bottom-up trait based approach might inform future trawling adaptation strategies, and tailor spatial conservation measures supporting an Ecosystem-Based Fisheries Management.

The use of rigorous methodologies to assess environmental, social and health impacts of specific interventions is crucial to disentangle the various components of environmental questions and to inform public opinion. The power of systematic maps relies on the capacity to summarise and organise the areas or relationships most studied, and to highlight key gaps in the evidence base. The recent Italian technical referendum (2016) – a public consultation inviting people to express their opinion by voting to change the rules on the length of licence duration and the decommissioning of offshore oil and gas platform drilling licences – inspired the creation of a systematic map of evidence to scope and quantify the effects of off-shore extraction platforms on Mediterranean marine ecosystems. The map was aimed as a useful model to standardise a "minimal informational threshold", which can inform public opinion at the beginning of any public consultation. Produced by synthesising scientific information, the map represents a reliable layer for any future sustainable strategy in the Mediterranean basin by: (i) providing a summary of the effects of marine gas and oil platforms on the Mediterranean marine ecosystem, (ii) describing the best known affected components on which the biggest monitoring efforts have been focused, and (iii) strengthening the science-policy nexus by offering a credible, salient and legitimate knowledge baseline to both public opinion and decision-makers. The map exercise highlights the knowledge gaps that need filling and taking into due consideration before future transnational and cross-border monitoring and management plans and activities can be addressed.

Physical disturbance by trawling can have both negative and positive effects on populations of scavenging benthic organisms. In the present study the impact of fishing activity on feeding behaviour of the two Astropectinids, Astropecten bispinosus and A. irregularis, was assessed based on stomach contents analysis. The study was carried out along trawled seabed highlighting the positive response of the two facultative scavengers to carrion generated by trawl disturbance. Furthermore, there was greater food specialization in areas that were more heavily exploited by trawling. This specialisation could be linked to the availability of certain prey that results from the passage of fishing gears across the seabed. Interestingly, differences between the two species analysed have been highlighted in term of population dynamic, feeding rate, diet composition and diet diversity, testifying their capacity to coexist in the same fishing grounds.