Avian olfaction has gained prominence in recent decades for its roles in social communication and behavior. In penguins, the chemical characterization of preen oil remains limited. In this exploratory study, we characterized the volatile organic compound (VOC) profile of preen oil from Humboldt Penguins (Spheniscus humboldti). Preen oil from 12 captive individuals was analyzed using headspace sorptive extraction (HSSE) coupled to gas chromatography–mass spectrometry (GC–MS). Chemometric analyses examined variation in VOC profiles by sex, age class, and breeding activity. We detected 54 compounds (20 tentatively identified), including linear alcohols, methyl ketones, carboxylic acids, saturated hydrocarbons, oxygenated and nitrogen-containing organics, prenolipids, and benzene derivatives. To our knowledge, this is the first study to apply HSSE–GC-MS to the volatile fraction of penguin preen oil. The analyses revealed VOC differences associated with sex, age class, and breeding activity in the study population. These findings suggest that VOCs in preen oil may serve as a valid matrix for future metabolomic studies, offering a noninvasive alternative for monitoring physiological status.

In view of the toxic effects of persistent organic pollutants (POPs), fast and effective assessment of their concentrations in marine mammals is important for understanding individual and population-level health impacts. This study developed an ultrasonic-based method that is less time-consuming, uses minimal solvent, and thus is more sustainable than the gold standard Soxhlet method for accurate analysis of organochlorine pesticides (OCs), polychlorinated biphenyls (PCBs), and benzene hexachlorides (BHC) in false killer whale blubber. This method was developed by comparing concentrations of POPs obtained using the traditional Soxhlet and novel ultrasonic extraction methods using blubber from false killer whales (n = 4) that were stranded in the Hawaiian Islands. The average total POPs extracted from the four killer whales and adjusted for lipid weight (lw) were 11,379.57 ± 3,303.03 ng/g lw for Soxhlet extraction and 14,310.39 ± 4,469.00 ng/g lw for the ultrasonic method, indicating a greater extraction efficiency of the ultrasonic method. The results further revealed that false killer whale FKW2013018 (∑OCs 15,447.38 ± 812.17 ng/g lw and ∑PCBs 5,205.32 ± 253.46 ng/g lw) and false killer whale FKW2016016 (∑OCs 18,164.90 ± 1,433.15 ng/g lw and ∑PCBs 4,913.32 ± 447.29 ng/g lw) accumulated organochlorines and PCBs at very high and potentially toxic levels. The low ratio of 4,4'-DDT/4,4'-DDE (0.026 ± 0.004) using both extraction methods indicated that the stranded false killer whales experienced long-term exposure to 4,4'-DDT, leading to bioaccumulation of the stable 4,4'-DDE metabolite. The levels of OCs, PCBs, and BHCs in this study were below toxic threshold levels. However, in view of the susceptibility of cetaceans with reduced lipid content to the toxic effects of POPs, cetaceans with low lipid content (as a result of starvation, fasting, or extended migration events) may be at higher risk of the negative health impacts of organic pollutants.

The current world's life-threatening illnesses have amplified multidrug resistance infections, bringing about immune system mayhem, thus, the quest for novel antimicrobial compounds with a broad spectrum of action. Four Ni(II) complexes, [Ni(YB)Cl]& sdot;2H2O (C1), [Ni(YB)Br]& sdot;H2O (C2), [Ni(YB)NO3]& sdot;3H2O (C3), [Ni(YB)COOCH3]& sdot; 2H2O (C4) [HYB = 4-[(1E)-N-{2-[(Z)-(4-methoxybenzylidene)amino]ethyl}ethanimidoyl]benzene-1,3-diol], were synthesized. Analytical techniques like CHNS analysis, UV-Vis, FT-IR, molar conductance, XRD, 1H NMR, and TGA/DTA were utilized for characterization. The calculated EHOMO - ELUMO energy gap and global reactivity descriptors of the compounds were performed by DFT calculations. The energy gap (Delta E) = EHOMO - EHOMO for the studied compounds HYB, C3, C4, C1, and C2 were found to be 1.736, 1.243, 1.221 1.217, and 1.193 eV respectively. The chelated complexes exhibited higher DPPH radical scavenging power than the corresponding free HYB ligand. Amongst the complexes, C2 displayed the highest scavenging ability (IC50 = 2.59 +/- 1.21 mu M). Antimicrobial activities of the synthesized compounds were validated against bacterial strains: gram (+) E. faecalis and S. aureus; gram (-) P. aeruginosa and K. pneumoniae; and fungi: C. neoformans and C. albicans. C2 exhibited the most inhibition (MIC = 390.6 mu g/mL) against P. aeruginosa and E. faecalis, while C1 acted as the most effective compound (MIC = 48.83 mu g/mL) against the fungi strains. The docking study illustrated the highest binding affinity of -7.30 kcal/mol by C2 with P. aeruginosa (PDB: 8BN6), and C1 for the C. neoformans with -6.04 kcal/mol (PDB ID: 7T08) binding sites. Potential binding modes around the receptor's active sites were predicted by the in silico molecular docking studies.

At the nanoscale level, several biological processes take place, owing to the potential that engineered nanomaterials might interrelate with bio-molecules and cellular procedures. This study aimed to synthesize cadmium oxide nanoparticles via a one-step calcination process of tetradentate Schiff base-Cd(II) complex at different temperature ranges. The as-synthesized compounds were carried out via a viz UV–visible, elemental analysis, 1H NMR, molar conductivity, transmission electron microscopy (TEM), FT-IR spectroscopy, and X-ray diffraction (PXRD). The band gap energy and average particle sizes of the CdO particles are respectively (2.69 eV, 3.54 eV), 26.88 nm for CdO@250, and (3.20 eV, 3.57 eV), 25.67 nm for CdO@300, while CdO@350 exhibited the 3.78 eV and 28.42 nm values. The antioxidant accomplishments of the test samples through the scavenging activity of DPPH radicals showed CdO@300 to possess (IC50 = 5.18 ± 0.56 µg/mL). Similarly, the as-synthesized CdO nanoparticles exhibited higher antibacterial activities against S. aureus and E. coli as compared to the corresponding Cd-HMB and ligand (HMB), while ciprofloxacin acted as a standard antibiotic. Furthermore, HMB and its complex Cd-HMB were docked against the DNA gyrase enzymes of S. aureus (PDB IDs: 5CDQ) and E. coli (PDB IDs: 6F86) as receptors. The binding sites docking results showed that the binding energies of HMB and Cd-HMB to 5CDQ ranged from − 3.44 to − 4.99 kcal/mol and from − 6.45 to − 6.64 kcal/mol, while the binding energies related to the target 6F86 are in the ranges of (− 3.64, − 4.76) kcal/mol and (− 6.08, − 6.09) kcal/mol respectively. Therefore, the significant antioxidant and antibacterial activities of the ligand (HMB), Cd-HMB, and CdO NPs review the broad application prospects of these compounds as therapeutic agents for wide-ranging biomedical applications.

In this study, trivalent metal complexes of the category: [M(L)(H 2 O) n Cl y ] obtained from the interaction of metal 3 + ion salts with organic N, N, O -Schiff base ( HL ) (where: HL = 4-{( Z )-((2-{( E )-((2-hydroxyphenyl)meth- ylidene)amino}ethyl)imino)methyl}-2-methoxyphenol; n, y = 1 or 2 and M = Ti(III), Fe(III), Ru(III), Cr(III) and Al(III)) were synthesized and characterized viz molar conductance, FT-IR, and UV - Vis spectroscopies, elemental analyses, thermal analyses (TGA and DTA), and UV - Vis spectroscopy, theoretical calculations. A distorted octahedral structure around the metal ions was proposed based on the obtained experimental and calculated data. Thermal examination of the complexes signposts the step-by-step disintegration to give the final decomposition product as metal oxides. Moreover, DFT calculations were executed utilizing the B3LYP/LANL2DZ theory level, which revealed that the synthesized metal (III) complexes were more stable than the free ligand ( HL ). The value of Delta E for HL is 4.60 eV while the related values for the complexes of Cr(III) ( C1 ), Ru(III) ( C2 ), Fe (III) ( C3 ), Al(III) ( C4 ), and Ti(III) ( C5 ) are respectively 2.59, 3.68, 3.15, 1.64, and 2.75 eV. Scavenging abilities of DPPH and ABTS radicals by the test compounds revealed promising antioxidant behavior. It was observed that the compounds are proficient DPPH radical scavengers in a dose-dependent configuration. Ru(III); IC 50 = 1.69 +/- 2.68 mu M for DPPH and Ti(III); IC 50 = 8.70 +/- 2.78 mu M for ABTS performed best. Similarly, the complexes demonstrated higher antimicrobial activities compared to HL against the designated strains, while ciprofloxacin acted as a standard antibiotic. Furthermore, the ligand and its most effective complexes C2 and C5 were docked against the targets S. aureus DNA gyrase ( 2XCT ), S. pneumoniae DNA gyrase ( 5BOD ), and E . coli DNA gyrase ( 5L3J ). The binding sites were evaluated and the docking results showed that the studied molecules bind to the targets through classical O - H ... O and/or N - H ... O hydrogen bonds, as well as via hydrophobic contacts.

Citrus greening disease, or Huanglongbing (HLB), has devastated citrus crops globally in recent years. The causal bacterium, 'Candidatus Liberibacter asiaticus', presents a sampling issue for qPCR diagnostics and results in a high false negative rate. In this work, we compared six metabolomics assays to identify HLB-infected citrus trees from leaf tissue extracted from 30 control and 30 HLB-infected trees. A liquid chromatography-mass spectrometry-based assay was most accurate. A final partial least squares-discriminant analysis (PLS-DA) model was trained and validated on 690 leaf samples with corresponding qPCR measures from three citrus varieties (Rio Red grapefruit, Hamlin sweet orange, and Valencia sweet orange) from orchards in Florida and Texas. Trees were naturally infected with HLB transmitted by the insect vector Diaphorina citri. In a randomized validation set, the assay was 99.9% accurate to classify diseased from nondiseased samples. This model was applied to samples from trees receiving plant defense-inducer compounds or biological treatments to prevent or cure HLB infection. From two trials, HLB-related metabolite abundances and PLS-DA scores were tracked longitudinally and compared with those of control trees. We demonstrate how our assay can assess tree health and the efficacy of HLB treatments and conclude that no trialed treatment was efficacious.

Biotransformation of organic pollutants is crucial for the dissipation of environmental pollutants. While the roles of microorganisms have been extensively studied, the significant contribution of various root exudates are still not very well understood. Through plant growth experiment, coupled with gas and liquid chromatography-mass spectrometry methods, this study examined the effect of the presence of M. sativa on microbial-associated biochemical transformation of petroleum hydrocarbons. The results of this study revealed that the concentration of exudates within the soil matrix is a function of proximity to root surfaces. Similarly, biodegradation was found to correlate with distance from roots, ranging from ≥ 90% within the rhizosphere to < 50% in bulk soil and unplanted control soil. Most importantly, for the first time in a study of an entire petroleum distillate, this study revealed a statistically significant negative correlation between root exudate concentration and residual total petroleum hydrocarbons. While not all the compounds that may influence biodegradation are derived from roots, the results of this study show that the presence of plant can significantly influence biodegradation of hydrocarbon pollutants through such root exudation as organic acids, amino acids, soluble sugars and terpenoids. Therefore, root exudates, including secondary metabolites, offer great prospects for biotechnological applications in the remediation of organic pollutants, including recalcitrant ones.

Most of the buzz surrounding the word "nanotechnology" has been on over the last 15–20 years. The application of nanotechnology in diverse fields is based on two motives. Firstly, an increasing surface/volume ratio, nanoparticles become more active. Secondly, a smaller particle size results in greater quantum confinement which is responsible for the changes in electron mobility, effective mass, relative dielectric constant, and optical properties. Application of nanotechnology in medicine (nanomedicine) includes Nanodiagnosis, Nano therapy (controlling drug delivery), and Remedial (renovated) medicine. Nano diagnosis is the evolution of nanodevices and image devices to detect and analyze diseased conditions, cellular malfunction, and unmoral cells. Nano therapy assigns effective nano-systems by performing a more effective therapy while minimizing side effects while renovated medicine aims to repair or reproduce deteriorated tissues and organs using nanotechnology materials. Although nanotechnology is a rapidly growing field, availability of product is far away from reach, this is because of various hurdles at different stages of development. Major hurdles needed to be overcomed include toxicity, production cost, environmental hazards, and accessibility to unreachable far-off areas. The obstacles to growth if overcome can cause ground-breaking modifications in the field of health care and medicine. It is hoped that this chapter will provide updated information useful for material scientists, biotechnologists, pharmacologists, clinicians, researchers in the health sector, or scholars of the allied fields, who have an interest in drug discovery and development, nano-diagnosis, nanobiotechnology, and nanomedicine.

Herbal medicine is a form of medicine that has been extensively exploited in traditional medicine, and its therapeutic potential is accepted. Combretum is one of the most frequently happening genera in the African and Asia tropical and subtropical areas; some are widely used in African herbal medicine due to their ethnopharmacological properties. Numerous species of this plant have been used and expended owing to high pharmaco-constituents following their phytochemical screening and evaluations. The recent incidence of multidrug-resistant strains and reduced receptiveness to antibiotics has raised serious anxiety in health delivery and the need for an urgent search for new antibiotics mediators from nature. A countless number of natural substances have resulted from the Combretum species as medicine and are utilized traditionally for the management of bacteriological infection. The plants have received comprehensive documentation as a good cradle of natural constituents that can be categorized into four groups following their biosynthetic source: alkaloids, terpenoids, polyketides, and phenylpropanoids. The study deals with the ethnobotanical and pharmacological significance of the Combretum species for treating numerous ailments and diseases.

Phytopharmacological review on Acanthospermum hispidumAnup K Chakraborty, Amit V Gaikwad, Karuna B Singh

Alternaria leaf spot of cabbage, caused by the Alternaria brassicicola, affects leaves of cabbages and often results in head rots causing severe decline in yield. In this work, the effects of systemic and combination fungicides on A. brassicicola mycelia growth in vitro and disease severity in field trials were investigated. The results of in vitro evaluation revealed that both fungicides significantly inhibited (p < 0.05) the growth of A. brassicicola under in vitro conditions. However, metalaxyl-M 6% was less effective with 100 μg/mL having only 30 ± 3.5% inhibition. On the other hand, 100 μg/mL of mancozeb 63% + carbendazim 12% had 94 ± 3.5% growth inhibition of A. brassicicola, respectively, under the same conditions. Dose-response analysis of the efficacy of the two fungicides showed that the LC50 of metalaxyl-M 6% and mancozeb 63% + carbendazim 12% were 125.52 ppm and 57.22 ppm, respectively, indicating the superiority of combination fungicide over systemic fungicide alone. Field studies showed that while manure type significantly impacted on biomass production (p < 0.001), it did not significantly affect disease severity. On the other hand, the frequency of fungicide application impacted on disease severity, with biweekly application leading to a significant reduction in disease severity after 10 weeks.

The most common approaches for the in-situ bioremediation of contaminated sites worldwide are bioaugmentation and biostimulation. Biostimulation has often proved more effective for chronically contaminated sites. This study examined the effectiveness of optimized water hyacinth compost in comparison with other organic and inorganic amendments for the remediation of crude oil-polluted soils. Water hyacinth was found to be rich in nutrients necessary to stimulate microbial growth and activity. An organic geochemical analysis revealed that all amendments in this study increased total petroleum hydrocarbon (TPH) biodegradation by ≥75% within 56 days, with the greatest biodegradation (93%) occurring in sterilized soil inoculated with optimized water hyacinth compost. This was followed by polluted soil amended with a combination of spent mushroom and water hyacinth composts (SMC + WH), which recorded a TPH biodegradation of 89%. Soil amendment using the inorganic fertilizer NPK (20:10:10) resulted in 86% TPH biodegradation. On the other hand, control samples (natural attenuation) recorded only 4% degradation. A molecular analysis of residual polycyclic aromatic hydrocarbons (PAHs) showed that the 16 PAHs designated by the US EPA as priority pollutants were either completely or highly degraded in the combined treatment (SMC + WH), indicating the potential of this amendment for the environmental remediation of soils contaminated with recalcitrant organic pollutants.

COVID-19 is caused by infection with the “severe acute respiratory syndrome coronavirus-2″ (i.e., SARS-CoV-2). This is an enveloped virus having a positive sense, single-stranded RNA genome; like the two earlier viruses SARS-CoV and the Middle East respiratory syndrome (MERS) virus. COVID-19 is unique in that, in the severe case, it has the propensity to affect multiple organs, leading to multiple organ distress syndrome (MODS), and causing high morbidity and mortality in the extreme case. In addition, comorbidities like age, cardiovascular disease, diabetes and its complications, obesity, are risk factors for severe COVID-19. It turns out that a most plausible, simple, single explanation for this propensity for MODS is the pivotal involvement of the vascular endothelium (VE). This is a consequence of the fact that the VE seamlessly connects all the entire vascular bed in the body, thus linking all the target organs (heart, lungs, kidney, liver, brain) and systems. Infection with SARS-CoV-2 leads to hyper-inflammation yielding uncontrolled production of a mixture of cytokines, chemokines, reactive oxygen species, nitric oxide, oxidative stress, acute phase proteins (e.g., C-reactive protein), and other pro-inflammatory substances. In the extreme case, a cytokine storm is created. Displacement of the virus bound to the VE, and/or inhibition of binding of the virus, would constitute an effective strategy for preventing COVID-19. In this regard, the acetone-water extract of the leaf of the Neem (Azadirachta indica) plant has been known to prevent the adherence of malaria parasitized red blood cells (pRBCs) to VE; prevent cytoadherence of cancer cells in metastasis; and prevent HIV from invading target T lymphocytes. We therefore hypothesize that this Neem leaf acetone-water extract will prevent the binding of SARS-CoV-2 to the VE, and therefore be an effective therapeutic formulation against COVID-19. It is therefore advocated herein that this extract be investigated through rigorous clinical trials for this purpose. It has the advantages of being (i) readily available, and renewable in favor of the populations positioned to benefit from it; (ii) simple to prepare; and (iii) devoid of any detectable toxicity.

The biotechnological application of microorganisms for rhizoremediation of contaminated sites requires the development of plant-microbe symbionts capable of plant growth promotion and hydrocarbon degradation. Here, we present a study aimed at isolating single microbial strains that are capable of promoting plant growth as well as rhizoremediation of diesel fuel hydrocarbons. Through genomic analyses and greenhouse-based experiments, we examined the synergistic interactions of Medicago sativa L. and Paraburkholderia tropica WTPI1 for enhanced rhizoremediation of diesel fuel-contaminated soils. Plant growth-based experiments confirmed that the inoculation of M. sativa with P. tropica led to a 99% increase in plant biomass. Furthermore, organic geochemical analysis revealed that 96% of all the distinctive diesel fuel hydrocarbons, including C 10 –C 25 n -alkanes, branched alkanes, cycloalkanes and aromatic hydrocarbons were degraded in the M. sativa + P. tropica treatment. These results will prove beneficial for biotechnological application of P. tropica WTPI1 for plant growth promotion and most importantly for environmental remediation of organic pollutants.

The slow rate of natural attenuation of organic pollutants, together with unwanted environmental impacts of traditional remediation strategies, has necessitated the exploration of plant-microbe systems for enhanced bioremediation applications. The identification of microorganisms capable of promoting rhizoremediation through both plant growth-promoting and hydrocarbon-degrading processes is crucial to the success and adoption of plant-based remediation techniques. In this study, through successive enrichments of soil samples from a historic oil-contaminated site in Wietze, Germany, we isolated a plant growth-promoting and hydrocarbon-degrading bacterial consortium dominated by Alphaproteobacteria. In microcosm experiments involving Medicago sativa L. and the isolated bacterial consortium, we examined the ability of the consortium to enhance rhizoremediation of petroleum hydrocarbons. The inoculation of M. sativa with the consortium resulted in 66% increase in plant biomass, and achieved a 91% reduction in diesel fuel hydrocarbon concentrations in the soil within 60 days. Metagenome analysis led to the identification of genes and taxa putatively involved in these processes. The majority of the coding DNA sequences associated with plant growth promotion and hydrocarbon degradation in this study were affiliated to Acidocella aminolytica and Acidobacterium capsulatum indicating their potential for biotechnological applications in the rhizoremediation of sites contaminated by petroleum-derived organic pollutants.

The Clean Air Act (42 U.S. Code § 7401) is one of the United States of America’s most influential environmental laws. Under the Clean Air Act Amendments of 1990, oxygen-containing organic compounds must be added to some fossil fuels with the goal of combating CO2 and particulate emissions. However, one major implication is the effect of co-solvency on the leaching potentials of polycyclic aromatic hydrocarbons (PAHs) into groundwater. Our research investigated this effect on three groups of recalcitrant PAHs that are present in diesel fuel. Our results reveal that ethanol addition enhances the leaching potentials of these otherwise hydrophobic contaminants, with 10% ethanol (E10) resulting in elution of all the PAHs studied. While 5% ethanol addition to diesel fuel resulted in the elution of an average of 2.5% of all the trimethylnaphthalenes and 6.0% of the C2 alkylphenanthrenes present in diesel fuel, 10% ethanol addition led to the elution of more than 80% of each of the studied trimethylnaphthalene peaks and more than 70% of each of the studied C2 alkylphenanthrene peaks present in diesel fuel. In view of the known mutagenic and carcinogenic risks associated with exposure to PAHs through groundwater contamination, our study highlights the need for energy scientists to carefully consider the environmental and health implications of ethanol-blended innovations holistically. It is not enough to save the atmosphere but ruin the hydrosphere and most importantly, human health.

Environmental contamination by petroleum hydrocarbons is of concern due to the carcinogenicity and neurotoxicity of these compounds. Successful bioremediation of organic contaminants requires bacterial populations with degradative capacity for these contaminants. Through successive enrichment of microorganisms from a petroleum-contaminated soil using diesel fuel as the sole carbon and energy source, we successfully isolated a bacterial consortium that can degrade diesel fuel hydrocarbons. Metagenome analysis revealed the specific roles of different microbial populations involved in the degradation of benzene, toluene, ethylbenzene and xylene (BTEX), and the metabolic pathways involved in these reactions. One hundred and five putative coding DNA sequences were identified as responsible for both the activation of BTEX and central metabolism (ring-cleavage) of catechol and alkylcatechols during BTEX degradation. The majority of the Coding DNA sequences (CDSs) were affiliated to Acidocella, which was also the dominant bacterial genus in the consortium. The inoculation of diesel fuel contaminated soils with the consortium resulted in approximately 70% hydrocarbon biodegradation, indicating the potential of the consortium for environmental remediation of petroleum hydrocarbons.

Summary Environmental contamination by petroleum hydrocarbons is of concern due to the carcinogenicity and neurotoxicity of these compounds. Successful bioremediation of organic contaminants requires bacterial populations with degradative capacity for these contaminants. Through successive enrichment of microorganisms from a petroleum-contaminated soil using diesel fuel as the sole carbon and energy source, we successfully isolated a bacterial consortium that can degrade diesel fuel hydrocarbons. Metagenome analysis revealed the specific roles of different microbial populations involved in the degradation of benzene, toluene, ethylbenzene and xylene (BTEX), and the metabolic pathways involved in these reactions. One hundred and five putative coding DNA sequences (CDSs) were identified as responsible for both the activation of BTEX and central metabolism (ring-cleavage) of catechol and alkylcatechols during BTEX degradation. The majority of the CDSs were affiliated to Acidocella, which was also the dominant bacterial genus in the consortium. The inoculation of diesel fuel contaminated soils with the consortium resulted in approximately 70% hydrocarbon biodegradation, indicating the potential of the consortium for environmental remediation of petroleum hydrocarbons.

The biotechnological application of microorganisms for rhizoremediation of contaminated sites requires the development of plant-microbe symbionts capable of plant growth promotion and hydrocarbon degradation. Studies focusing on microbial consortia are often difficult to reproduce, thereby necessitating the need for culturable single bacterial species for biotechnological applications. Through genomic analyses and plant growth experiments, we examined the synergistic interactions of Medicago sativa L. and Paraburkholderia tropica for enhanced remediation of diesel fuel-contaminated soils. Comparative genomics revealed strong potential of P. tropica for plant growth-promotion, chemotaxis and motility, root nodulation and colonization, and diesel fuel degradation. Plant growth experiments confirmed that P. tropica thrived in the contaminated soils and effectively enhanced M. sativa growth. Geochemical analysis showed that the M. sativa + P. tropica treatment resulted in an efficient degradation of diesel fuel hydrocarbons within two months, offering great prospects for enhanced biodegradation of organic pollutants. ### Competing Interest Statement The authors have declared no competing interest.

The slow rate of natural attenuation of organic pollutants, together with unwanted environmental impacts of traditional remediation strategies, has necessitated the exploration of plant-microbe systems for enhanced bioremediation applications. The identification of microorganisms capable of promoting both plant growth and hydrocarbon degradation is crucial to the success of plant-based remediation techniques. Through successive enrichments of a soil sample from a historic oil-contaminated site in Wietze, Germany, we isolated a plant growth-promoting and hydrocarbon-degrading bacterial consortium. Metagenome analysis of the consortium led to the identification of genes and taxa putatively associated with these processes. The majority of the coding DNA sequences involved in these reactions were affiliated to Acidocella aminolytica and Acidobacterium capsulatum. In microcosm experiments performed in association with Medicago sativa L., the consortium achieved 91% rhizodegradation of diesel fuel hydrocarbons within 60 days, indicating its potential for biotechnological applications in the remediation of sites contaminated by organic pollutants.