IntroductionThis study analyzed injury epidemiology and incidence in male youth futsal players competing in the top national category over a regular season. The aim was to quantify injury incidence, prevalence, and burden, and to describe injury distribution according to exposure (training vs. match), body region, injury type, severity, and playing position.MethodsA prospective cohort study was conducted during the 2023–2024 season in the Spanish Youth Division of Honour, the highest national Under-19 futsal league in Spain. The sample comprised 164 male players from 13 teams who were followed prospectively over a nine-month period. Injury data were recorded throughout the season, whereas training and match exposure were estimated based on the standard schedule of the participating teams. Playing position, dominant leg, injured body region, injury type, severity, and time-loss duration were also recorded. Injury incidence, prevalence, and burden were subsequently calculated.ResultsA total of 111 injuries were registered, affecting 79 players (48% of the cohort), resulting in an overall incidence of 4.96 injuries per 1,000 hours of exposure. Match incidence (53.84/1,000 h) was substantially higher than training incidence (1.94/1,000 h). Most injuries involved the lower limbs (80.1%), particularly the ankle (23.4%) and knee (20.7%). Muscle/tendon (36.9%) and ligament (36.0%) injuries were the most frequent, and wingers sustained the highest number of injuries (n = 51). Median time loss was 16 days, while mean time loss reached approximately 36 days, and injury burden reached 176.5 days per 1,000 hours.ConclusionThese findings indicated a considerable injury risk in this population, especially during matches, and supported the need for targeted prevention strategies focused on strength development, neuromuscular control, and load management.

BACKGROUND: Inspiratory muscle training (IMT) has been widely used to enhance inspiratory muscle strength and pulmonary function. It may, therefore, have a role improving execution of wind musicians, however, this is largely unknown. This study investigated the impact of IMT on woodwind and brass players. METHODS: A cross-over randomized controlled study was conducted. Data on peripheral oxygen saturation, heart rate, electromyography-EMG, forced expiratory volume in the first second-FEV1, forced vital capacity-FVC and the ratio between FEV1/FVC, maximum inspiratory pressure-MIP, rate of perceived exertion and self-perceived levels of dyspnea were gathered before and after the intervention. Participants were also questioned about their instrumental study routines, daily habits and their experience with the IMT. RESULTS: Fourteen wind players, seven males and seven females, were included. Significant improvements in MIP were registered for time (F-(1.26)=18.0, P<0.001, eta(2)(p)=0.409) and for the interaction between time and groups (F-(1.26)=12.9, P=0.001, eta(2)(p)=0.331), with an increase in MIP scores favoring the EG. For the rate of perceived exertion a decrease was observed during performance with a significant difference, for time (F-(1.26)=6.6, P=0.016, eta(2)(p)=0.203) and for the interaction between time and groups (F-(1.26)=5.1, P=0.033, eta(2)(p)=0.163), favoring the EG. CONCLUSIONS: This study showed significant improvements in the MIP, decrease in the rate of perceived exertion after five-weeks of IMT in wind players. IMT may be effective in improving respiratory muscle strength in wind players.

Work-related musculoskeletal disorders are prevalent in occupations requiring prolonged standing and repetitive movements, often leading to vascular issues and reduced static balance. Innovations in wearable technology, such as smart footwear integrating active systems, aim to mitigate these challenges. This exploratory study assessed the effects of a novel active footwear system, incorporating compression and vibration, on vascular blood flow and static balance in healthy adults. Sixteen healthy participants (seven men and nine women) were randomized into active and placebo phases, each involving repetitive tasks. Outcomes included reflection photoplethysmography, postural sway, and foot volumetry. Data were analyzed pre- and post-intervention, with statistical significance set at p < 0.05. For men, significant improvements in reflection photoplethysmography median values were observed post-active phase (p = 0.031), while women showed no change. Enhanced static balance, reflected in decreased total sway (p = 0.025), was noted in women. No significant changes occurred during the placebo phase. The active system improved vascular function in men and static balance in women, highlighting its potential for ergonomic interventions in industrial settings. Future studies should explore long-term effects and applications in diverse populations, including those with work-related musculoskeletal disorders.

IntroductionThe objective of this study was to investigate the perceived impact of the different phases of the menstrual cycle on football and futsal participation and identify barriers that may limit the performance and participation of Portuguese female players across competition levels.MethodsAn online survey was conducted with the following inclusion criteria: registered participation in official football or futsal Portuguese leagues, an age of 18 years or older, and perceived regular menstrual cycles. A total of 197 answers were obtained and analyzed.ResultsThe majority of participants were 18-25 years old (61%), and 59% played futsal. Top-tier league players represented 15% of respondents, mid-tier 26%, and low-tier 59%. For the majority of respondents, the first 3 days of menstruation were perceived as having the most negative impact (66%), with abdominal pain, bloating, and breast tenderness being the most common symptoms. Players also associated the menstrual phase with decreased performance (endurance and power) and self-confidence, whereas they generally felt more confident and motivated during ovulation. No significant differences were found between sports (football and futsal) or competition levels in the perceived impact on performance and participation (p > 0.05). Overall, 53% of the players reported a perceived lack of knowledge and trust in their coach and other staff members to talk openly about menstrual health. The most cited external barrier to sports participation was the fear of leaking. To address the identified challenges, five intervention levels were proposed: communication, comprehension, education, equipment, and facilities.DiscussionThese findings emphasize the need for better communication, education, and structural support to reduce menstruation-related barriers.

Concepts underlying the so-called Industry 4.0 are an opportunity to develop strategies based on the merging of the physical, digital, and biological worlds, enabled by new technologies. This work reports the design proposal for an upside body wear involving industry workers to capture body signs, including real-time postural assessment, that could indicate risk for musculoskeletal disorders. Discover, Research, Engage, Approve, Make methodology allows us to consider factors such as situations, contexts, company employees, and workers (pe, gender, age, or type of task) to have a complete representation of the system. A series of participatory observations were performed in the industrial context which contributed to establishing a deeper relationship between the stakeholders involved around the theme with the analytical portion of the model enabling work in tandem with industrial partners. From participatory observation and worker feedback, the team developed a soft wearable to monitor the body conditions to prevent work-related injuries, instead of an exoskeleton to enhance the body. Nevertheless, the design team started to create concepts of soft wearables, always paying attention to the limitation of the components being used by the upper body device, designing where the circuits would be located around the body, resorting to a life-sized dummy as a model, and testing its arm movements. Future developments include the integration of several partial prototypes that will generate a final device that will integrate all the functions.

Work-related musculoskeletal disorders, a major public health problem, are mainly caused by the task nature and the work environment. One of the most important risk factors for the development of work-related musculoskeletal disorders is muscle fatigue. Therefore, its assessment can contribute to the application of preventive measures. Many studies have evaluated muscle fatigue associated with work tasks in laboratory settings, but they do not mimic industrial environments, with their constant noise, air pollution, and stress. A repetitive task of vertical screw-tightening performed by an experienced worker in a manufacturing and assembling industry was selected. Four assessment moments took place during the shift on 3 non-consecutive days. Outcomes included electromyography data from the upper trapezius and biceps brachii to assess muscle fatigue, painful symptoms in the upper limbs’ joints and trunk, and perceived exertion. An increase in painful symptoms and perceived exertion was reported by the worker throughout each of the analyzed shifts. A trend toward increased electromyography values was also observed. Muscle tension in the upper trapezius seems to have an inverse association with tension in the biceps brachii in this activity. Although it is possible to verify a trend towards an increase in electromyography, this trend is timid, with variability between the different cycles.

Anterior cruciate ligament (ACL) rupture is one of the most common knee injuries among athletes (Evans and Nielson, 2022). ACL reconstruction is, in general, the solution for the rupture of ACL; the torn ligament is replaced with a tissue graft from another surrounding functional structure of the knee (Rochmania et al., 2012). The functional changes that occur after ACL reconstruction comprise deficits of muscle power, functional performance, joint proprioception, and balance (Ageberg, 2002). Evidence shows that even several months after a successful ACL reconstruction subjects still present motor control changes, influencing knee functionality (Furlanetto et al., 2016).

Objective:This study aimed to describe and characterize injuries sustained by elite male futsal players in Portugal.Design:Prospective cohort study.Setting:Top-tier Portuguese league in the 2019 to 2020 season.Participants:One hundred sixty-seven players from 9 elite/international-level (tier 4) futsal teams.Independent variables:The location, type, body side, body part, mechanism of injury, severity, occurrence, days lost, training, and match exposure were collected.Main Outcome Measures:Injury incidence, prevalence, and burden.Results:The study was conducted during an 8-month season. A total of 133 injuries were recorded, and 92 (67.6%) players sustained injuries. The overall time-loss injury incidence was 4.5 injuries per 1000 hours of exposure. Injury incidence during matches was higher than during training sessions (25.9 vs 3.0 per 1000 hours of exposure, respectively). Average time loss was 9 days, and moderate injuries were the most frequent (44%), followed by mild injuries (24%). Injury burden was 73.8 days lost per 1000 hours of total player exposure. Sprains/ligament (29%) and muscle rupture/tear/strains (32%) were the most common injuries. The groin (19%), thigh (17%), knee (19%), and ankle (15%) were the most affected body areas. Noncontact injuries were the most reported mechanism (65%), and 24% were overuse injuries.Conclusions:This study showed that elite/international-level (tier 4) male futsal players are more prone to noncontact injuries, primarily affecting the lower limbs. The incidence during match play increased by 9-fold compared with training sessions.

BACKGROUND:Proteostasis impairment and the consequent increase of amyloid burden in the myocardium have been associated with heart failure (HF) development and poor prognosis. A better knowledge of the protein aggregation process in biofluids could assist the development and monitoring of tailored interventions. AIM:To compare the proteostasis status and protein's secondary structures in plasma samples of patients with HF with preserved ejection fraction (HFpEF), patients with HF with reduced ejection fraction (HFrEF), and age-matched individuals. METHODS:A total of 42 participants were enrolled in 3 groups: 14 patients with HFpEF, 14 patients with HFrEF, and 14 age-matched individuals. Proteostasis-related markers were analyzed by immunoblotting techniques. Fourier Transform Infrared (FTIR) Spectroscopy in Attenuated Total Reflectance (ATR) was applied to assess changes in the protein's conformational profile. RESULTS:Patients with HFrEF showed an elevated concentration of oligomeric proteic species and reduced clusterin levels. ATR-FTIR spectroscopy coupled with multivariate analysis allowed the discrimination of HF patients from age-matched individuals in the protein amide I absorption region (1700-1600 cm-1), reflecting changes in protein conformation, with a sensitivity of 73 and a specificity of 81%. Further analysis of FTIR spectra showed significantly reduced random coils levels in both HF phenotypes. Also, compared to the age-matched group, the levels of structures related to fibril formation were significantly increased in patients with HFrEF, whereas the β-turns were significantly increased in patients with HFpEF. CONCLUSION:Both HF phenotypes showed a compromised extracellular proteostasis and different protein conformational changes, suggesting a less efficient protein quality control system.

BACKGROUND:In manufacturing industries, tasks requiring poor posture, high repetition, and long duration commonly induce fatigue and lead to an increased risk of work-related musculoskeletal disorders. Smart devices assessing biomechanics and providing feedback to the worker for correction may be a successful way to increase postural awareness, reducing fatigue, and work-related musculoskeletal disorders. However, evidence in industrial settings is lacking.OBJECTIVE:This study protocol aims to explore the efficacy of a set of smart devices to detect malposture and increase postural awareness, reducing fatigue, and musculoskeletal disorders.METHODS:A longitudinal single-subject experimental design following the ABAB sequence will be developed in a manufacturing industry real context with 5 workers. A repetitive task of screw tightening of 5 screws in a standing position into a piece placed horizontally was selected. Workers will be assessed in 4 moments per shift (10 minutes after the beginning of the shift, 10 minutes before and after the break, and 10 minutes before the end of the shift) in 5 nonconsecutive days. The primary outcomes are fatigue, assessed by electromyography, and musculoskeletal symptoms assessed by the Nordic Musculoskeletal Questionnaire. Secondary outcomes include perceived effort (Borg perceived exertion scale); range of motion of the main joints in the upper body, speed, acceleration, and deceleration assessed by motion analysis; risk stratification of range of motion; and cycle duration in minutes. Structured visual analysis techniques will be conducted to observe the effects of the intervention. Results for each variable of interest will be compared among the different time points of the work shift and longitudinally considering each assessment day as a time point.RESULTS:Enrollment for the study will start in April 2023. Results are expected to be available still in the first semester of 2023. It is expected that the use of the smart system will reduce malposture, fatigue, and consequently, work-related musculoskeletal pain and disorders.CONCLUSIONS:This proposed study will explore a strategy to increase postural awareness in industrial manufacturing workers who do repetitive tasks, using smart wearables that provide real-time feedback about biomechanics. Results would showcase a novel approach for improving self-awareness of risk for work-related musculoskeletal disorders for these workers providing an evidence base support for the use of such devices.INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID):PRR1-10.2196/43637.

This study characterizes the plasma levels and composition of SDS-resistant aggregates (SRAs) in patients with heart failure with preserved ejection fraction (HFpEF) to infer molecular pathways associated with disease and/or proteostasis disruption. Twenty adults (ten with HFpEF and ten age-matched individuals) were included. Circulating SRAs were resolved by diagonal two-dimensional SDS-PAGE, and their protein content was identified by mass spectrometry. Protein carbonylation, ubiquitination and ficolin-3 were evaluated. Patients with HFpEF showed higher SRA/total (36.6 ± 4.9

The claim that the effects of kinesiology tape are different depending on the direction of tape application needs to be clearly ascertained. This study aimed to determine the immediate effects of two forearm kinesiology tape applications on muscle tone, stiffness, and elasticity of young individuals. Thirty-nine participants (15 men and 24 women) were randomized (1:1:1) to: the facilitatory group, receiving kinesiology tape applied from origin to insertion; the inhibitory group, receiving kinesiology tape applied from insertion to origin; or, a control group, without any intervention. The mechanical properties - tone, elasticity, and stiffness - of the forearm muscles were measured with a handheld mechanical impulse-based myotonometric device before and 30 min after the kinesiology tape application. Only the application of kinesiology tape from origin to insertion significantly increased muscle tone [16.6 (2.5) to 17.4 (3.5) Hz, p = 0.036], stiffness [318.3 (52) to 355.0 (87) N/m, p = 0.004], and elasticity [0.98 (0.1) to 1.10 (0.1), p = 0.023]. No changes were observed in both inhibitory kinesiology tape and the control group. In conclusion, kinesiology tape application has different effects depending on the direction of the taping application. The facilitatory tapping increased muscle tone, elasticity, and stiffness.

Abstract Funding Acknowledgements Type of funding sources: Other. Main funding source(s): MG and CS were supported by a PhD FCT grant (SFRH/BD/128893/2017) and by an individual grant from CAPES [BEX 0554/14-6], respectively. This work was financially supported by the project POCI-01-0145-FEDER-030011, funded by FEDER, through COMPETE2020-POCI, and by national funds, through FCT/MCTES (PTDC/MEC-CAR/30011/2017). iBiMED is a research unit supported by the Portuguese Foundation for Science and Technology (REF: UID/BIM/04501/2020) and FEDER/Compete2020 funds). Introduction Amyloid-like protein aggregates play a decisive role in the pathology of heart failure. Alterations in protein homeostasis, in particular, the clearance of toxic amyloid-like aggregates are emerging therapeutic targets in cardiovascular medicine. The clinical benefits of cardiac rehabilitation and exercise training are widely accepted in heart failure; however, little is known about the potential benefit of exercise training in amyloid-like protein aggregates. Purpose To assess the effects of a moderate-intensity exercise training program on amyloid-like protein aggregates levels among patients with heart failure with reduced ejection fraction. Methods Eighteen subjects participated in the study; eight patients (age: 66.6 ± 5.9 years; FEVE: 38.4 ± 8.9%) with heart failure with reduced ejection fraction participated in a 3-month exercise training program (2 x 60 min sessions per week of moderate-intensity aerobic and resistance exercise). Ten healthy subjects (age: 68. 4 ± 3.1 years) were recruited to an age-matched reference group. Amyloid-like protein aggregates were assessed before and after 3 months of exercise training. Clinical data, medication, anthropometrics, and cardiorespiratory fitness were also assessed. Thioflavin T (ThT) dye fluorescence was used to quantify the plasma levels of amyloid-like aggregates and the Fourier transform infrared spectroscopy (FTIR) was applied to evaluate the conformation of cross-β-sheet structures characteristic of amyloid protein aggregates. Results Exercise program improved cardiorespiratory fitness by 14.0 ± 17.1% (17.4 ± 3.2 to 19.7 ± 2.9 ml/kg/min) and reduced NT-proBNP levels by 16.5% (34.2) (median concentration of 632 pg/mL (720.8) to 517.5 pg/mL (707.0)) in the heart failure patients. A slight decrease of amyloid-like aggregates levels was observed in post-exercise training samples (a reduction of 3.1%); interestingly, after the exercise training program, the heart failure patients showed levels of amyloid-like aggregates similar to the reference group (1132.0 ± 114.2 vs. 1094.8 ± 132.9 a.u.). Additionally, the PLS-R multivariate analysis of the amide I region of the FTIR spectra revealed enrichment of antiparallel β-sheets (1693 cm-1) assigned to amyloid-like oligomers in the samples of heart failure patients before, but not after, the exercise program. Of note, oligomeric species, as intermediates of amyloid assembly, can contribute to the increase of amyloid burden, but also, some have been reported to be highly reactive and toxic to cells, being key elements of amyloid pathogenesis. Conclusions Our preliminary results indicate that 3 months of exercise training may have significant effects on amyloid-like oligomers, and start hindering the formation of the larger ThT-positive aggregates among patients with heart failure. Abstract Figure.

Objective: To compare the structural conformation of circulating protein aggregates and the levels of SDS-resistant protein aggregates between patients with heart failure with preserved ejection fraction (HFpEF) and age-matched individuals. Design and method: Ten adults were recruited, five patients with HFpEF (age: 70.0 ± 3.2 years, FEVE: 58.0 ± 7.5%, NT-proBNP: 1039.4 ± 530.7 pg/mL, BMI: 33.2 ± 5.6 kg/m2, SBP: 142.0 ± 23.5 mmHg, DBP: 74.6 ± 9.3 mmHg) and five age-matched individuals with cardiovascular risk factors (70.6 ± 2.7 years, BMI: 32.8 ± 2.8 kg/m2, SBP: 38.5 ± 13.8 mmHg, DBP: 81.6 ± 6.6 mmHg). SDS detergent-insoluble protein aggregates were analysed in plasma pre-cleared from albumin and immunoglobulin, by diagonal two-dimensional electrophoresis. Fourier-transform infrared spectroscopy (FTIR) was used to assess plasma proteins’ secondary structures, particularly the ones related with high aggregation propensity. Results: Our results indicate that HFpEF patients have similar plasma levels of SDS-resistant protein aggregates to those of age-matched participants (29.6 ± 6.4 vs. 35.0 ± 3.9%, p = 0.153). However, HFpEF patients with lower VO2peak and higher NT-proBNP generally presented higher level of protein aggregates. FTIR multivariate analysis of the amide region I (1700–1600 cm-1), which is directly related to proteins secondary structures, allowed to discriminate HFpEF from age-matched group. Partial Least Square Regression (PLS-R) to this spectra region showed that HFpEF patients exhibited strong vibrational peaks characteristic of protein aggregates (intermolecular beta-sheet (1625 cm-1), anti-parallel beta-sheet (1693 cm-1) and aggregate strands (1611 cm-1)); the age-matched individuals were characterized by a band containing alpha-helix structures (1653 cm-1), characteristic of a more stable conformation with less susceptibility to aggregation. Conclusions: The analysis of the protein aggregation profile by FTIR revealed that the plasma from HFpEF patients was characterized by the presence of secondary structures typical of protein aggregates. Although the fraction of detergent-insoluble aggregates was not different from the one of age-matched participants, our ongoing identification of proteins present in the aggregates, by mass spectrometry, will allow us to infer main molecular pathways involved in the progression of HFpEF.

Many contaminant yeast strains that survive inside fuel ethanol industrial vats show detrimental cell surface phenotypes. These harmful effects may include filamentation, invasive growth, flocculation, biofilm formation, and excessive foam production. Previous studies have linked some of these phenotypes to the expression of FLO genes, and the presence of gene length polymorphisms causing the expansion of FLO gene size appears to result in stronger flocculation and biofilm formation phenotypes. We performed here a molecular analysis of FLO1 and FLO11 gene polymorphisms present in contaminant strains of Saccharomyces cerevisiae from Brazilian fuel ethanol distilleries showing vigorous foaming phenotypes during fermentation. The size variability of these genes was correlated with cellular hydrophobicity, flocculation, and highly foaming phenotypes in these yeast strains. Our results also showed that deleting the primary activator of FLO genes (the FLO8 gene) from the genome of a contaminant and highly foaming industrial strain avoids complex foam formation, flocculation, invasive growth, and biofilm production by the engineered (flo8∆::BleR/flo8Δ::kanMX) yeast strain. Thus, the characterization of highly foaming yeasts and the influence of FLO8 in this phenotype open new perspectives for yeast strain engineering and optimization in the sugarcane fuel-ethanol industry.

Many contaminant yeast strains able to survive inside fuel ethanol industrial vats show detrimental cell surface phenotypes, such as filamentation, invasive growth, flocculation, biofilm formation and excessive foam production. Previous studies have linked some of these phenotypes to the expression of FLO genes, and the presence of gene length polymorphisms causing the expansion of FLO gene size appears to result in stronger flocculation and biofilm formation phenotypes. We have performed here a molecular analysis of FLO1 and FLO11 gene polymorphisms present in contaminant strains of S. cerevisae from Brazilian fuel ethanol distilleries showing strong foaming phenotypes during fermentation. The size variability of these genes was correlated with cellular hydrophobicity, flocculation and highly foaming phenotypes in these yeast strains. Our results also show that deleting the major activator of FLO genes (the FLO8 gene) from the genome of a contaminant and highly foaming industrial strain avoids problematic foam formation, flocculation, invasive growth and biofilm production by the engineered (flo8∆::BleR / flo8Δ::kanMX) yeast strain. Thus, the characterization of highly foaming yeasts and the influence of FLO8 in this phenotype opens new perspectives for yeast strain engineering and optimization in the sugarcane fuel-ethanol industry.

Residential consumers have been adopting distributed energy resources (DER) like photovoltaics (PV), electric vehicles (EV) as well as electric heating, ventilation and air conditioning devices (HVAC) in recent years - thus substantially reshaping power systems. This study is dedicated to the analysis of such adopters in continental Portugal, using both spatial analysis tools and census data with information theoretic criteria. Results suggest that the current uptake of EV, PV, and HVAC is characterised by spatially auto-correlated adoption patterns. The analysis of census variables, on the other hand, reveals that Portuguese EV, PV, and HVAC adopters exhibit a few surprising, unrecorded characteristics compared with previous studies. Comparing different dataset resolutions, EV and HVAC adopters are found to be most similar across all three aggregation levels considered. Results further show that fewer adopter groups tend to own both EV-HVAC and PV-HVAC, reducing per se synergy potentials that may arise behind the metre. One of the main outcomes from this work is that studies describing energy technology adopters using census variables might receive very unstable results across different data aggregation levels. This may lead to adverse effects on studies' conclusiveness and energy policy design choices.

Background: Futsal is considered to be the world's fastest growing indoor sport. Being a multiple-sprints sport, with a high intensity level of play, the players are submitted to high risk of injury. The fast growth in the rates of futsal participation is increasing the awareness and concern regarding the public health significance of futsal injuries. Objectives: This narrative review aims to provide an overview of the specificities of futsal, injury incidence in futsal, and current evidence regarding the effects of injury prevention programs, focusing on the FIFA 11+, in futsal and football. Major Findings: Futsal involves a considerable number of high-intensity phases in terms of proportion of match time, which may help to justify a high rate of injuries in futsal. The most prevalent time-loss injuries in futsal are the ankle sprain, groin and thigh strains. Injury prevention programs have led to significant reduction in injuries amongst football players. The majority of studies performed so far in football players to analyze the risk of injuries with the FIFA11+ showed significant risk reductions; there is a clear need for studies in futsal players, as only one study was identified showing a reduction of overall, acute and lower limb injuries during the season. Four studies assessed the impact of the FIFA11+ on physical performance in futsal players, showing positive results in knee muscle strength. Conclusions: Considering that the main injury in futsal is the ankle sprain, the injury risk reduction observed in football and futsal players after the FIFA 11+, and the positive effects of the program in some physical parameters (e.g. muscle strength) in futsal players, it seems reasonable to suggest the use of the FIFA 11+ to reduce injury risk in futsal players.

Objective To examine the effectiveness of the FIFA 11+ in reducing injury in futsal players. Methods Ninety-one male futsal players from six amateur futsal clubs were recruited and assessed for eligibility, and 71 were randomized to the 11+ group (n = 37, age: 27.0 +/- 5.1 years) and a control group (n = 34, age: 26.0 +/- 5.1 years). The 11+ program was executed twice a week for 20 weeks, separated by a 10-week period where both groups executed their regular warm-up. Data on match, training exposure, and injuries were recorded during the regular season. Results The players sustained a total of 58 injuries during the futsal regular season, with 24 injuries in the 11+ group and 34 injuries in the control group; the overall incidence of injuries per 1000 player-hours was significantly higher in the control group (11.6 vs 6.5; mean difference (95% CI) -5.1 (-9.1 to -1.1), P = .014). The 11+ group had a significantly lower incidence of acute (11.2 vs 5.7; -5.5 (-9.4 to -1.6), P = .007) and lower limb (8.7 vs 4.4; -4.2 (-8.1 to -0.4), P = .032) injuries per 1000 player-hours. Players from the control group had a higher number of days injured (20.4 +/- 17.3 vs 10.5 +/- 9.1, P = .036). Conclusion The FIFA 11+ is an injury prevention program suited for injury reduction in amateur futsal players, as it reduces the incidence of overall, acute, and lower limb injuries during the season.