Poultry manure is used as fertilizer in most parts of the world. It can harbor diverse nature of pathogenic bacteria and may be one of the active sources of resistant pathogens transmissions. Current study was planned to identify the pathogens as well as their antibiotic resistance profile being isolated from chicken's manure. In present study total (n = 60, 100%) fresh fecal samples were collected from commercial chicken's farms of District Kohat. Samples were initially cultured on different agar media and were incubated at 37 degrees C overnight. Further antibiotics susceptibility and characterization of resistance genes were performed for all the selected pathogens. Only (n = 50, 83.3%) of the total isolates showed proper growth. Most frequent pathogens were Escherichia coli (19, 38%) followed by Salmonella (13, 26%), Klebsiella (8, 16%), Shigella (7, 14%) and Pseudomonas (3, 6%). E. coli revealed highest resistance to doxycycline (16, 84.2%), followed by ampicillin (15, 78.9%), amoxicillin (14, 73.7%) and ciprofloxacin (13, 68.5%). Salmonella showed complete resistance to doxycycline (13, 100%) and tetracycline (11, 84.6%), ciprofloxacin (5, 38.5%) and amoxicillin (4, 31.8%). Pseudomonas were completely resistance to ampicillin followed by levofloxacin (2, 66.7%) and ciprofloxacin (1, 33.3%). Klebsiella showed highly resistance to tetracycline (8, 100%), levofloxacin (6, 75%), ciprofloxacin (4, 50%) and amoxicillin (4, 50%). Shigella strains were completely resistant to doxycycline (7, 100%) and ampicillin (6, 85.7%) while showed less resistance to ciprofloxacin (3, 42.8%) and amoxicillin (2, 28.6%). In the present study, four classes of antibiotics resistance genes (tetA, sul1, str and aadA) were identified with different frequency. The highest frequency (100%) was found for tetA gene followed by the sul1 and str genes (66.6%) each. The lowest frequency (16.6%) was noted for (aadA) gene. It has been concluded that all the isolated pathogens were highly resistant to doxycycline, amoxicillin and erythromycin. To restrict the transmission of resistant pathogens in the environment, it is needed to dispose of poultry manure in proper way, to educate staff of personal hygiene and justified use of antibiotics.

The present study comprises of 16 indigenous hybrids with two replications and evaluated in RCBD during Kharif 2023 to assess the hybrids for kernel yield and its associated traits at Faisalabad, Punjab, Pakistan. Analysis of variance revealed that plant height (PHt), ear height (ErHt), ear length (ErL), ear girth (ErG), kernel/row (KeRo), kernel length (KeL), kernel width (KeW), kernel thickness (KeTh), 100 kernel weight (KW), shelling % (Shell) showed highly significant variation, kernel yield (Y) possessed significant variation while days to 50% silking (Silk) showed non-significant variation among 16 hybrids. Pearson correlation analysis revealed that highly significant association found between PHt and KeW (0.72**), KW and ErL (0.68**), KeRo and KeTh (-0.64**). Significant correlation found between KW and KeRo (0.53*), ErL and KeRo (0.52*), ErG and KeL (0.57*), KW and KeTh (-0.51*), KeL and KeTh (-0.53*). The first two components of PCA accounted for 53.2 % of the total variance. PCA biplots arrow for Y aligns in the direction of ErG and KeL that showed significant association for Y improvement. Arrow head of PHt and ErHt point in the same direction, suggesting both traits can be improved simultaneously but they are oriented opposite to Y. Similarly, arrows of ErG and KeL point in the same direction, connecting positive relation between them which can be improved together. Kernel yield (Y) can be enhanced by improving ErG and KeL. Finally, FH-1720 and YH-5427 exhibited superior performance in Y, KeL and ErG, making them promising candidates for achieving higher grain yield.

Current studies were carried out to evaluate the synergistic effect of selected essential oils when combined or mixed with alpha cypermethrin to reduce the insecticide dose as well increase the mixture efficacy in controlling adult Bactrocera zonata. Essential oil tested included Parthenium hysterophorus (PH), Eucalyptus obliqua (EO), Cannabis sativa (CS) each one used at five different concentrations selected as multiple (0.25, 0.5, 1, 2, 4) of their LC 50 values determined on the basis of preliminary trials. Constant ratio combination design was used for mixing the individual components. Different component mixtures included combinations of (PH + CS), (PH+EO), (EO+CS), (PH+CS+EO) each mixed at five concentration levels (same levels). Similarly, each of the essential oil was mixed with alpha Cypermethrin in combinations of multiple (0.25, 0.5, 1, 2, 4) of their LC 50 values. Toxicity of individual components as well as mixtures was determined by using dry film residue method. The response to Toxicity response exposure was estimated using the median-effect equation, as described by Chou and Talalay (1984). Interactions of these mixtures were studied using the combination-index (CI) equation method using the software Compusyn that is based on general equation of dose and effect and its theorem of combination index developed by using the approach of merging the physicochemical principle of the mass-action law with the mathematical principle of induction and deduction. LC values of individual essential oils as determined by the model were 0.55, 0.398 and 0.572 for C. sativa, P. hysterophorus and E. obliqua respectively. Cypermethrin exhibited LC50 value of 0.00353. When binary combination of cypermethrin and c. sativa were tested, the value of CI ranged between 0.53-1.74. (<1 being synergism) was observed only for the mixture combining 0.25(LC50) but the effect size was small (fa=0.6). All the binary combinations of cypermethrin alpha and P. hysterophorus showed antagonistic effect only. Cypermethrin when combined with E. obliqua indicated synergism at 0.25(LC50) and 4(LC50) with effect size of 0.68% and 0.9% respectively. In combination mixing all the three Eos with pyrethroids showed synergism only at highest concentration 4(LC50) with a very high effect size (fa=0.99). When Eos were mixed to see their synergistic effect, combination of P. hysterophorus with E. obliqua. Showed a moderate synergism (CI= 0.77) at the lowest concentration tested 0.25 (LC50) but the effect size was relatively smaller (fa=0.52). All the combination of C. sativa and E. obliqua showed antagonism (CI=1.26-2.09). In a binary combination of P. hysterophorus and C. sativa, a high level of synergism was observed at the highest concentration combination 4(LC50) with a very high effect size (fa=0.99). When all the three Eos were combined, Synergism was observed at 2(LC50) and 4(LC50) combinations with a high affect size (fa=>0.98).

Wheat (Triticum aestivum L.) is a crucial global cereal crop, but its yield is severely affected by Leaf rust (LR) caused by Puccinia triticina. LR results in reddish-brown lesions on wheat leaves, which disrupt photosynthesis and reduce crop productivity. This study identifies three novel quantitative trait loci (QTLs) associated with LR resistance in wheat and evaluates optimal cross combinations within a segregating F2 population. Yield-related traits including 1000-grain weight, peduncle length, spike length, and grain yield per plant were evaluated over three replications. The parental genotypes, AN179, AN1687, PR123, and PR127, demonstrated high potential for both yield and reduced LR infection rates. Among the crosses, AN179 x PR127 was the most effective, exhibiting healthier grains, higher grain counts per spike, longer peduncles for better photosynthate translocation, and adult plant resistance to slow rusting. Disease severity ranged from 2.9 % to 29.1 % in the parental genotypes and from 4.7 % to 51.1 % in the crosses. Parents PR123, PR127, AN179, and AN1687 showed resistance to field disease severity. The crosses AN176 x PR127, AN1687 x PR123, and AN179 x PR123 exhibited high resistance, while AN179 x PR127 showed the lowest field disease severity at 4.7 %. A linkage map of the F2 population revealed three novel LR resistance QTLs on chromosomes 2AS, 1BL, and 6B in the cross AN179 x PR127. Microsatellite markers Xgwm273, Xgwm610, and Xgwm493 were closely linked to these QTLs. These novel QTLs can be utilized in wheat hybridization programs for marker-assisted selection and pyramiding of all-stage LR genes to enhance resistance.

Wheat production faces great threat due to rising environmental temperatures resulting from climate change. Here we analyzed a bulk of wheat exotic, elite, synthetic and local wheat lines to evaluate the impact of heat stress on the physiological and biochemical parameters of different genotypes. Mutant susceptible genotypes were generated by treating with Ethyl Methane Sulfonate (EMS) and its performance was evaluated under normal and heat stress conditions. Based on the heat stress index and yield measurement, the lines were grouped into four classes, i.e. tolerant (T), Susceptible (S), moderately tolerant (MT), and moderately susceptible (MS). The results showed significant impact of heat stress on all parameters of the tested gene pool; however, the affect was less intense on the tolerant lines compared to the other classes. To validate heat stress tolerance, we conducted RNA sequencing analysis and identified eight genes associated with heat tolerance. Among them, TaDWF4˗4B showed highest expression under heat stress condition and selected for further functional analysis. Overexpression of TaDWF4˗4B in the wheat line ESWYT-4 enhanced heat tolerance. Treatment with 2 μM brassinosteroids (BR) decreased seed germination in the transgenic lines, suggesting that TaDWF4˗4B enhances BR response. Endogenous BR contents increased in overexpression lines, along with increasing the expression of several BR biosynthetic pathway genes in overexpression line under heat stress condition. Moreover, the overexpression of TaDWF4˗4B improved reactive oxygen species (ROS) scavenging by increasing the activities of TaCAT3, TaSOD1, and TaGPx1-D under heat stress condition. These findings indicate that TaDWF4˗4B plays an important role in regulating BR biosynthesis, increasing BR response, and ROS scavenging under heat stress condition. These results present mechanistic insights into the role of TaDWF4˗4B in plant responses under heat stress condition.

Rust diseases are among the most devastating threats to global wheat production significantly impact the crop productions as well as food security. Identification and deployment of rust resistance genes is critical for developing resistant wheat varieties. The premeditated crops urged to be screened regularly for the most tolerant and stable genotypes selections which are incompatible with the surrounding environmental. The present research trials were processed by screening the (66) NUWYT wheat genotypes with Rusts result. The rusts of wheat, occurred due to three species of Puccinia, are very shocking disease results and are main biotic constraints in efforts to sustain wheat production worldwide. Stripe rust is a widely spread disease of wheat, resulting in significant losses in yield and quality. Each monogenic line of Yr1, Yr5, Yr10, Yr15, Yr17, Yr32 and YrSP showed higher levels of resistance to these races, at seedling period. In present research work we utilized these markers for yellow rust CSLV34 Lr34/Yr18, S19M93-140 Yr5, Xbarc181 for Yr18 gene, Xgwm-498 Yr24/26 all give the significant results. The objective of the present study was the identification of Lr genes in 66 wheat cultivars in Pakistan. Two Markers have been utilized for Lr genes detection in 66 wheat lines that is SCS265512 Lr19 and SCAR-WMC44 Lr46. The most frequently reporting genes in 66 wheat cultivars were Lr19, It is concluded that there was a good variation in Lr genes carried by wheat cultivars commercially grown in Pakistan. These findings were for stem rust identification and resistance gens Sr22 and Sr36 in 66 NUWYT wheat cultivars. Two SSR markers Xwmc633, Xgwm319 were used for detecting the Sr22 and Sr36. This study investigates the identification of rusts resistance genes in wheat by using molecular markers. By going through the marker-assisted selection (MAS), we enabled to evaluate the several wheat genotypes for rust resistance genes confirmation. Results revealed that molecular markers like SSRs (Simple Sequence Repeats), Marker CSLV34 Lr34/Yr18, S19M93-140, Xbarc181 for Yr18 gene, Xgwm-498 Yr24/26, SCS265512 Lr19, SCAR-WMC44 Lr46, Xwmc633 Sr22 and Xgwm319 Sr36 are effectively identified the resistance genes such as Lr, Yr, and Sr and providing the valuable tools for wheat breeding programs. These markers should be helpful in marker-assisted pyramiding of rusts resistance genes for developing new cultivars along with various genes resistance against stem rust, leaf rust and yellow rust races in Pakistan. The resistance resources investigated in current study can help and guide wheat breeders to pyramid Yr, Sr and Lr resistant genes to bring about new wheat genotypes.

ABSTRACT Leaf rust (LR) poses a global threat to wheat crops and can lead to severe yield losses if environmental conditions favour its spread. Using resistant wheat cultivars offers a sustainable approach to managing LR. This study aimed to identify promising wheat lines for LR‐resistance breeding using classical analytical methods to screen for LR tolerance. We evaluated 10 parental lines, comprising 6 lines and 4 testers, crossed into 24 combinations using a line × tester mating design. These germplasm were grown in a triplicate RCB design under both optimal and LR‐stress conditions. We recorded data on various morphological, physiochemical, yield and component traits at key growth stages. The analysis of combining ability indicated significant variations among genotypes, with non‐additive gene action influencing most traits. Four promising parents (AN179, AN1687, PR123 and PR127) and two crosses (AN179 × PR127 and AN179 × PR123) showed high combining ability for yield traits under LR‐stress. Cluster analysis revealed divergent groups among the genotypes, with shifting clustering under LR‐stress suggesting varied genotypic responses. Factor analysis identified genotypes that performed consistently well under LR‐stress. These genotypes are suitable for use in LR‐resistance breeding programs. We also recommend peduncle length and tillers per plant as phenotypic markers for wheat selection and breeding due to their positive correlation with grain yield. The findings of this study can contribute valuable insights to sustainable wheat breeding research.

The research studies were conducted on insect pests in the Hazara division (Haripur, Abbottabad, Mansehra, and Battagram) on rice under the proposed project. During the visit, five fields were selected in each district to record major and minor insect pests on rice. After 10 days, the visit to the crop in each district was repeated. In NARC, the preserved insects were identified. In the present study, we recorded pests on rice that were Scirpophaga incertulas (Yellow stem borer), Armyworm, Nephotettix virescens (Green leafhopper), Leptocorisa acuta (Rice ear head bug), Brevennia rehi (Rice mealybug), Cnaphalocrocis medinalis (Rice leaf folder), Pyrilla perpusilla (Leafhopper), Nymphula depunctalis (Rice caseworm), and Reciliadorsalis (Zigzag leafhopper) from the above-mentioned districts. The seasonal average of each insect in each district varied in incidence, and the present study was conducted in 2023 in the Hazara region of Pakistan.

Purpose: The purpose of this research was to assess the production potential of maize by examining its agro-physiological traits under different combinations of organic and inorganic nitrogen fertilizers, as well as to evaluate the resulting changes in soil properties. Material and Methods: The study was conducted for two years using randomized complete block split-plot arrangement. Three tillage systems (minimum, conventional and deep) were used in the main plots, while ten different nitrogen treatments, with different proportions of single and combined applications of chemical fertilizer, poultry manure and bioslurry, were used in sub-plots. Further, detail is given in section-2. Findings and Recommendations: The treatment where deep tillage was used in combination with 50% of the recommended nitrogen from chemical fertilizer (67.5 kg ha-1), 25% from poultry manure (1.9 t ha-1) and 25% from bioslurry (2.1 t ha-1) showed the greatest increase in agro-physiological crop growth compared to the control (zero nitrogen application with minimum tillage), with a 76% increase in leaf area index (75 days after sowing), 86% increase in average mean crop growth rate and 38% increase in mean net N assimilation rate. This might be due to a 43% increase in recovery efficiency of fertilizer nitrogen in this treatment compared to the control. This treatment also decreased soil bulk density by 5% and increased soil porosity by 6%, total soil nitrogen by 21%, available soil phosphorous by 55% and available soil potassium by 20% compared to the control. Implications to Theory, Practice and Policy: These results suggest that, in this soil, this treatment provides the optimum nutrient management, both improving soil properties and achieving maximum agro-physiological growth due to enhancing fertilizer nitrogen recovery efficiency.

Parthenium is an invasive weed and has adverse effect on agriculture, biodiversity and human health in our ecosystem. This experiment was carried out to find the allelopathic water stress of parthenium effect on maize growth dynamics using 3 sigmoidal growth model. Soil was collected from weed infested field. Allelopathic water stress (AWS) greatly reduced weed density as compared to control. Allelopathic water stress of parthenium at higher concentration (PWS-100%) reduced maize germination and affect chlorophyll content, leaf area, plant height, shoot biomass, root biomass. As at lower concentration (AWS-25% and 50%) enhance maize growth parameters. This study revealed that allelopathic water stress of parthenium at lower concentration increase maize growth and decrease a weed density up to minimum level.

Tomato is an important vegetable crop grown on all continents of the world. Tomato mosaic virus disease significantly lowers the yield in the field every year. The present study was conducted to find the resistant germplasm and to evaluate the potential of different plant extracts to manage the Tomato mosaic virus (ToMV) disease in field conditions. Ten tomato genotypes were evaluated against the ToMV disease in field conditions. Out of ten varieties/lines, no one was found highly resistant and two varieties/lines (VRI-575 and Naqeeb) were evaluated as resistant to the disease. Aqueous extracts of three different plants (Azadirachta indica, Calotropis procera and Allium cepa) were potentially evaluated at 1% concentrations by three-times foliar application against the disease. In control, nothing was applied. A significant difference in disease index was observed among plant extracts. The disease significantly decreased with the number of applications. Azadirachta indica was found most effective to manage the disease as compared to the others in field conditions. Allium cepa was found least effective against the ToMV disease. From the present study, it was revealed that Naqeeb and VRI-575 varieties/lines may be used for the future breeding program and extracts of Azadirachta indica may be applied to manage the disease.

A pot experiment was conducted in the greenhouse to assess the effect of wheat straw derived biochar on the bioavailability of Pb, Cd and Cr using maize as test crop. Surface (0–20 cm) soil collected from the research farm of The University of Agriculture Peshawar was amended with 0, 2, 4 and 6% biochar respectively. Ten kg soil was placed in pots and all pots were spiked with Pb, Cd and Cr at concentrations 0, 5, 10 and 50 mg kg−1 of each element. The experiment was conducted in a completely randomized design, replicated three times. Maize seeds were sown in pots in wetter conditions. Soil and plant samples were taken at harvest time. Results show that soil Pb, Cd and Cr reduced from 15.5, 5.38 and 5.85 mg kg−1 in control to 1.34, 0.69 and 0.75 mg kg−1 respectively with application of 6% biochar when values were averaged over the contamination levels. Similarly, Pb, Cd and Cr accumulation in plant were reduced by the application of biochar as compared to control. As compared to control, there was a significant improvement in the plant growth with biochar at elevated levels of Pb, Cd and Cr in soil. Overall, the application of wheat straw biochar might be effective in immobilization of metal in the soil and reducing its uptake to plant. However, investigation of the residual effect of biochar is required for sustainable use of biochar for remediation of contaminated soil.

Insect control for crops is one of the most critical global concerns. Pest management is an economic and ecological problem worldwide due to the human and environmental risks raised by most synthetic pesticide products. Botanical insecticides have resurfaced in popularity due to their low cost and low environmental impact, rather than their negative effects on human health. Botanical insecticides destroy only the insects they are meant to kill, leaving no residue on food or in the environment. Botanicals have long been used to combat pests. The compounds have many environmental advantages. However, as opposed to other bio-control pests and pathogens, their use was minimal during the twentieth century. In developing countries, botanical insecticides are well adapted for use in organic food production. Nonetheless, they may play a far bigger role in developed countries’ food production and post-harvest food protection. Consequently, the current chapter briefly addresses botanicals with active ingredients with insecticidal, antifeedant, or repellent properties.

The beet armyworm Spodoptera exigua (Hübner) is an important pest of field and vegetable crops in Pakistan. Due to persistent use of insecticides, it developed high levels of resistance to organophosphates and pyrethroids in the mid 2000s and farmers then switched to new chemistries. In the present study, its resistance to new chemistries such as indoxacarb, spinosad, chlorfenapyr, methoxyfenozide and avermectins was monitored from 1998 to 2017 using a leaf-dip bioassay. There was generally a very low to low resistance to these insecticides during the first 11 years of their introduction (1998-2008). Resistance to methoxyfenozide remained very low throughout the 20 years of monitoring. A moderate resistance was recorded to indoxacarb (2011-2015), spinosad (2009-2012), chlorfenapyr (2011-2017), abamectin (2016-2017) and emamectin benzoate (2010-2013). A high resistance was found to indoxacarb during 2016–2017, spinosad during 2013–2017 and emamectin benzoate during 2014–2017. A rotation of diverse chemistries, having novel modes of action, along with other integrated pest management tactics, is recommended for the management of insecticide resistance in S. exigua.

Pea leaf miner Phytomyza atricornis Goureau (Diptera: Agromyzidae) is a major insect pest of pea in Peshawar, causing heavy losses to it each year. Synthetic insecticides have been routinely used for the control of leaf miner on pea, but there are many side effects with the regular use of synthetics on pea crop. In the present study efficacy of a biopesticide and three synthetic insecticides were compared against P. atricornis of pea during 2005. The results revealed that overall mean density of P. atricornis after 1 st and 2 nd chemical treatment was significantly lower in megamos (1.08, 1.59 individuals leaf -1 ) and larsban (1.47, 2.07 individuals leaf -1 ). In control overall mean density and mean density after each chemical treatment of P. atricornis remained significantly higher than all the insecticidal treatments. Pea yield was significantly higher in lorsban (3323 kg ha-1) and lower in control (2352 kg ha-1). The findings of the present research might help in better control of insect pest of pea crop in Peshawar.

Four tobacco cultivars viz. Flue-cured Virginia (FCV), Sun-cured Rustica (SCR), Dark Air-cured (DAC) and Air-cured Burley (ACB) were evaluated for preference by larvae and adults of Lasioderma serricorne. Leaf thickness (LT), moisture content (MC), nicotine, total sugars (TS), starch, chlorides and potassium contents were determined and the impact of these factors on beetle preference was worked out. FCV tobacco was highly preferred by both larvae (2.18) and adults (1.49) due to high percentages of MC (12.50), TS (15.84) and starch (6.89). ACB was least preferred due to low contents of MC (11.40%), TS (1.29%) and starch (1.14%). Potassium had negatively significant effect on the beetle’s preference. Regression analyses revealed a positive relationship of larvae and adults with MC (0.780, 0.803) TS (0.866, 0.713) and starch (0.888, 0.765) while a negative relationship with potassium (-0.684, -0.407). LT (0.155, 0.101) nicotine (-0.047, -0.277) and chlorides (0.163, 0.305) had non-significant association with preference by both larvae and adults respectively. The relationship between MC, TS and starch is direct curvilinear as their increase had encouraged the population of L. serricorne

A study on losses due to Brevicoryne brassicae in 12 Brassica genotypes under screen house conditions was conducted under screen house conditions during 2006-08. For the experiments artificialrninfestation of 5, 10 and 15 aphids/plant at flower-bud initiation stage was done in all the 12 genotypes. The results of the experiment of 5 aphids/plant infestation revealed that aphid density was significantly higher (106.2 aphidrnplant-1) on Rainbow and lower (27.67 aphid plant-1) on Raya Anmol and Peela Raya (30.17 aphid plant-1). In the experiment of 10 aphids plant-1 infestation, mean density of aphid was significantly higher on Legend (125.7 aphids plant-1) and Westar (117.0 aphids plant-1) while lower on Raya Anmol (57.67 aphids plant-1) and T-16-401 (54.50 aphids plant-1). In the experiment of 15 aphids aphids plant-1, mean aphid density was significantly higher (200.70 aphids plant-1) on Rainbow and lower (82.83 aphids plant-1)rnon Ganyou-5. In control (0 aphids aphids plant-1), Raya Anmol yielded significantly higher (7.32, 7.33 and 7.33 g plant-1) and Oscar lower (3.62, 3.62 and 3.60 g plant-1) in the 5, 10 and 15 aphids plant-1, respectively. Among the 5, 10 and 15 aphid infested plants, Ganyou-5 (4.98, 4.57, 3.45 g plant-1) yielded significantly higher and while Oscar (2.55, 1.60, 1.22) lower, respectively. The percent yield losses were significantly higher in Crusher (43.83%) and Torch (44.50%) and lower (11.08%) in T-16-401 in 5 aphid infested plant; significantly higher (62.15%) in Vanguard and lower in Legend (23.65%) and T-16-401 (22.09%) in 10 aphid/plant, and significantlyrnhigher (75.88%) in Vanguard and lower (29.14%) in T-16-401. The present results might be helpful in using host plant resistance in an integrated pest management program against B. brassicae on Brassica genotypes.

In the present study aphids density and yield components of 12 genetically diversified Brassica genotypes were determined under field conditions during 2006-08. The genotypes tested were Brassica napus (Westar), B. napus (Ganyou-5), B. napus (Rainbow), B. napus (Oscar), B. napus (Vanguard), B. napus (Crusher), B. napus (Torch), B. napus (Legend), B. napus (Altex), B. juncea (Raya Anmol), B. carinata (Peela Raya) and B. campestris (T-16-401). The results showed that mean aphids density was significantly higher (37.94 aphids plant-1) on Legend and lower (12.84 aphids plant-1) on Vanguard. With respect to yield and its components, highest values for seed yield (2386 kg/ha), plant height (214.3cm)rnand number of branches/plant (19.20) were recorded in Peela Raya, whereas maximum 1000 grain weight (4.80g), siliqua length (8.31cm) and seed/siliqua (23.57 seed) were found in Westar, Ganyou-5, and Oscar, respectively. Overall, among the 12 genotypes Vanguard, Crusher and Legend were the best. Based on comparatively better performance against aphids attack and giving better yield components, Vanguard, Crusher and Legend were found best among the Brassica genotypes.