Dorsal root ganglia from rats were incubated with 3-O-methyl-[14C]glucose, and [3H]leucine in the presence or absence of insulin in order to determine whether insulin influences the uptake of glucose and amino acids by the cells of the ganglion. No effect was detected. A significant proportion (38%) of the uptake of [3H]leucine was shown to be inhibited by ouabain and therefore energy dependent, utilizing Na+K+-ATPase. The activity of this enzyme is known to be impaired in dorsal root ganglia in diabetic rats, as is the uptake of amino acids; these phenomena are therefore unlikely to be due to a direct effect of reduced circulating insulin levels. The relevance of these findings to theories as to the causation of diabetic neuropathy is discussed.

The permeation of native and non-enzymatically glycated albumin and immunoglobulin G into the endoneurium of the sciatic nerve of rats was examined in acute experiments. Low amounts of native albumin entered both in control and streptozotocin-diabetic animals with no significant difference between them. The entry of glycated albumin was significantly greater both in control and diabetic rats, especially in the former. Permeation of native and glycated immunoglobulin G was not detectable over the time course of the experiment. It is concluded that glycation of albumin enhances its permeation into the nerve. This may be relevant to the increased amounts of endoneurial albumin that are detectable in human diabetic neuropathy.

Streptozotocin (STZ)-induced diabetes in the rat causes a significant reduction in ouabain-sensitive Na,K-ATPase pumping activity measured by 86Rb+ influx, in sciatic endoneurium (by 54%) and dorsal root ganglia (by 22%). For endoneurium, the change is similar to that of ouabain-sensitive enzymatic Na,K-ATPase activity (42%), but in dorsal root ganglia, the decrease in enzymatic Na,K-ATPase activity was much greater. 86Rb+ efflux from dorsal root ganglia showed no difference between diabetic and control animals, confirming that the abnormal 86Rb+ influx reflects Na,K-ATPase function and not abnormal membrane permeability. The significance of these findings to pathogenetic mechanisms in diabetic neuropathy is discussed.

Observations were made on the polypeptide and glycoprotein composition of dorsal root ganglia from streptozotocin-induced diabetic rats by sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE). Silver staining of one-dimensional gels failed to demonstrate any differences between diabetic and control animals. In two-dimensional studies, good resolution of polypeptides with a mass greater than 70 kDa was not obtained, but a number of important abnormalities in the polypeptide composition of diabetic ganglia were detected. Some polypeptides recognized in the gels from control ganglia were present in high concentrations in diabetic ganglia; other polypeptides, particularly a number of basic polypeptides of low molecular mass, were only identified in the diabetic rats. Three major polypeptides showed a small shift in their isoelectric point in the diabetic animals. The glycoprotein content of the ganglia was examined by lectin binding to both one- and two-dimensional gel separations. An increase in total glycoprotein content was evident in the diabetic ganglia. A number of polypeptides with a molecular mass between 70 and 110 kDa showed heavy glycosylation. Altered glycosylation of some specific polypeptides of lower molecular mass was also seen, 7 of these showing increased and 3 reduced glycosylation. The significance of these findings is discussed.

Conference Article| October 01 1988 Lack of correlation between enzymic Na+, K+-ATPase activity and 86Rb+ uptake in peripheral nerve tissue of diabetic rats N. J. PATEL; N. J. PATEL 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar J. G. LLEWELYN; J. G. LLEWELYN 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar D. W. WRIGHT; D. W. WRIGHT 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar P. K. THOMAS P. K. THOMAS 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1988) 16 (5): 878. https://doi.org/10.1042/bst0160878 Article history Received: April 06 1988 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation N. J. PATEL, J. G. LLEWELYN, D. W. WRIGHT, P. K. THOMAS; Lack of correlation between enzymic Na+, K+-ATPase activity and 86Rb+ uptake in peripheral nerve tissue of diabetic rats. Biochem Soc Trans 1 October 1988; 16 (5): 878. doi: https://doi.org/10.1042/bst0160878 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search © 1988 Biochemical Society1988 Article PDF first page preview Close Modal You do not currently have access to this content.

Conference Article| June 01 1988 Na/K-ATPase activity in peripheral nervous tissue of galactosaemic and diabetic rats N. J. PATEL; N. J. PATEL 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar J. G. LLEWELYN; J. G. LLEWELYN 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar P. K. THOMAS P. K. THOMAS 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, U.K. Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd Received: December 01 1987 Online ISSN: 1470-8752 Print ISSN: 0300-5127 © 1988 Biochemical Society1988 Biochem Soc Trans (1988) 16 (3): 306–307. https://doi.org/10.1042/bst0160306 Article history Received: December 01 1987 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation N. J. PATEL, J. G. LLEWELYN, P. K. THOMAS; Na/K-ATPase activity in peripheral nervous tissue of galactosaemic and diabetic rats. Biochem Soc Trans 1 June 1988; 16 (3): 306–307. doi: https://doi.org/10.1042/bst0160306 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: STZ, streptozotocin This content is only available as a PDF. © 1988 Biochemical Society1988 Article PDF first page preview Close Modal You do not currently have access to this content.

Conference Article| August 01 1987 Protein synthesis and amino acid uptake by dorsal root ganglia from diabetic rats N. J. PATEL; N. J. PATEL 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PN, U.K. Search for other works by this author on: This Site PubMed Google Scholar J. G. LLEWELYN; J. G. LLEWELYN 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PN, U.K. Search for other works by this author on: This Site PubMed Google Scholar D. W. WRIGHT; D. W. WRIGHT 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PN, U.K. Search for other works by this author on: This Site PubMed Google Scholar P. K. THOMAS P. K. THOMAS 1Department of Neurological Science, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PN, U.K. Search for other works by this author on: This Site PubMed Google Scholar Biochem Soc Trans (1987) 15 (4): 695–696. https://doi.org/10.1042/bst0150695 Article history Received: November 26 1986 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Facebook Twitter LinkedIn Email Cite Icon Cite Get Permissions Citation N. J. PATEL, J. G. LLEWELYN, D. W. WRIGHT, P. K. THOMAS; Protein synthesis and amino acid uptake by dorsal root ganglia from diabetic rats. Biochem Soc Trans 1 August 1987; 15 (4): 695–696. doi: https://doi.org/10.1042/bst0150695 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsBiochemical Society Transactions Search Advanced Search Keywords: STZ, streptozotocin, TCA, trichloracetic acid © 1987 Biochemical Society1987 Article PDF first page preview Close Modal You do not currently have access to this content.

The rate of protein synthesis was estimated in structurally preserved perikaryal preparations from 8-day-old rat cerebellum under conditions which overcome the problems of intracellular compartmentation. The rates were lower than the in vivo estimates at comparable ages, but they were of similar magnitude, and very much higher than previous estimates on isolated cells. Protein synthesis rate depended on the cell type. When expressed per cell the rank order in the preparations enriched in the indicated classes of cells was: Purkinje cells > astrocytes > granule cells in the S, G2 and M phase of the cell cycle > granule cells in G1 and G0. However, after normalizing the results for size differences between cell types, by expressing the rates in terms of unit protein or as a percentage replacement of the protein bound amino acid, astrocytes and replicating granule cells displayed greater rates than the Purkinje cells. The resolution of labelled proteins using SDS-PAGE indicated marked differences in the rate of synthesis of particular proteins. The results were consistent with the view that certain polypeptides are uniquely expressed in particular cell classes.