Hyperspectral optical observations of the Earth’s surface oceans from space offer a means to improve our understanding of ocean biology and biogeochemistry. NASA’s Plankton, Aerosol, Cloud, ocean Ecosystem (PACE) satellite mission, which includes a hyperspectral ocean color instrument (OCI), will provide radiometric observations of surface ocean with near continuous spectral resolution across the near UV to NIR range. Maintaining sufficient accuracy over the lifetime of satellite ocean color missions requires a robust program for system vicarious calibration (SVC) and product validation. The system vicarious calibration process combines satellite sensor data with in-situ radiometric/optical measurements to remove potential biases due to the combined errors from both satellite radiometric sensor calibration and atmospheric correction. As such, high accuracy, high-spectral resolution in-situ radiometric measurements are required to provide a principal source of truth for the satellite-derived products. To meet the requirements, a novel in-situ radiometric system, called HyperNav, has been developed, rigorously characterized and field tested. Key attributes of HyperNav are dual upwelling radiance heads coupled to individual spectrometers, spectral resolution of ∼2.2 nm (full width, half-maximum) across 320–900 nm, integrated shutter systems for dark measurements, and integrated tilt and pressure sensors. The HyperNav operational modes include traditional profiling and surface modes, as well as integration with an autonomous profiling float for unattended deployment, offering a new capability for a network of autonomous platforms to support the long-term needs for hyperspectral ocean color remote sensing observations. This paper describes the HyperNav design, in-situ operational modes, and field verification results.

Abstract. The air-sea CO2 flux in the coastal ocean is a key component of the global carbon budget. However, due to the scarcity of data, the many sources and sinks of carbon and their complex interactions in these waters remain poorly understood. In 2021, the Tara schooner collected 14,000 km of CO2 fugacity (fCO2) measurements along the coast of South America, including in the Amazon River-Ocean continuum (https://doi.org/10.5281/zenodo.13790065, Olivier et al., 2024a). The interactions between the Amazon River and its oceanic plume are complex, and under a combined influence of many processes such as tides and bathymetry. Downstream of the Amazon River plume, the fCO2 is low compared with that of the atmosphere, reaching a minimum of 42 μatm. In the river, fCO2 reaches up to 3000 μatm. South of the estuary, the waters of the North Brazil Current have a fCO2 exceeding 400 μatm. Along the Brazil Current, fCO2 is around 400 μatm and decreases, as does temperature, as the schooner sails away from the equator. Nevertheless, in all the data collected in this coastal environment, salinity varies greatly, and therefore describes best the variability of fCO2. Despite the strong variability and uncertainties in the data, comparison with discrete samples of other carbonate parameters shows that the mean differences (2 µatm) are within the range of uncertainties related to the chemical formula used for the comparison. This data set helps to fill the gap in our knowledge of the behavior of fCO2 in the under-sampled region of the Brazilian coast.

The composition of the marine phytoplankton community has been shown to impact many biogeochemical processes and marine ecosystem services. A variety of methods exist to characterize phytoplankton community composition (PCC), with varying degrees of taxonomic resolution. Accordingly, the resulting PCC determinations are dependent on the method used. Here, we use surface ocean samples collected in the North Atlantic and North Pacific Oceans to compare high performance liquid chromatography (HPLC) pigment-based PCC to four other methods: quantitative cell imaging, flow cytometry, and 16S and 18S rRNA amplicon sequencing. These methods allow characterization of both prokaryotic and eukaryotic PCC across a wide range of size classes. PCC estimates of many taxa resolved at the class level (e.g., diatoms) show strong positive correlations across methods, while other groups (e.g., dinoflagellates) are not well captured by one or more methods. Since variations in phytoplankton pigment concentrations are related to changes in optical properties, this combined dataset expands the potential scope of ocean color remote sensing by associating PCC at the genus- and species-level with group- or class-level PCC from pigments. Quantifying the strengths and limitations of pigment-based PCC methods compared to PCC assessments from amplicon sequencing, imaging, and cytometry methods is the first step toward the robust validation of remote sensing approaches to quantify PCC from space.

Ocean color satellites require a procedure known as System Vicarious Calibration (SVC) after launch as the pre-launch and on-orbit calibration accuracy is insufficient. The current approach for determination of post-launch SVC uses a single fixed measurement location and may be susceptible to unexpected biases in satellite processing algorithms. Here we describe a novel SVC program which is based on a high resolution and high accuracy radiometric system integrated with an autonomous profiling float (providing a buoyancy engine, physical observations, and communication). This float + radiometer (HyperNav) system can be shipped via air, land, ocean and is deployable from small boats. This SVC program relies on multiple deployment sites with associated facilities to collect a significant amount of SVC quality data in a relatively short time. It has centralized logistics and command-and-control centers ensuring easy access to information regarding the status of each asset and to ensure floats stay within a certain ocean area. The development of the program has been associated with the launch of NASA’s PACE satellite and has been executed by academic institutions in collaboration with an industrial partner. Other approaches for a future float-based operational SVC program are discussed.

Checking the radiometric calibration of satellite hyper-spectral sensors such as the PACE Ocean Color Instrument (OCI) while they operate in orbit and evaluating remote sensing reflectance, the basic variable from which a variety of optical and biogeochemical ocean properties can be derived, requires measuring upwelling radiance just above the surface (Lw) and downwelling planar irradiance reaching the surface (Es). For this, the current HyperNav systems measure Lw at about 2 nm spectral resolution in the ultraviolet to near infrared, but Es in only four 10 nm wide spectral bands centered on 412, 489, 555, and 705 nm. In this study, the Es data acquired in these spectral bands in clear sky conditions are used to reconstruct via a multi-linear regression model the hyper-spectral Es signal at 0.5 nm resolution from 315 to 900 nm, the OCI spectral range, allowing an estimate of Es at the HyperNav, OCI, and other sensors’ resolutions. After correction of gaseous absorption and normalization by the top-of-atmosphere incident solar flux, the atmospheric diffuse transmittance is expressed as a linear combination of Es measured in those 4 spectral bands. Based on simulations for Sun zenith angles from 0 to 75° and a wide range of (i.e., expected) atmospheric, surface, and water conditions, the Es spectrum is reconstructed with a bias of less than 0.4% in magnitude and an RMS error (RMSE) ranging from 0% to 2.5%, depending on wavelength. The largest errors occur in spectral regions with strong gaseous absorption. In the presence of typical noise on Es measurements and uncertainties on the ancillary variables, the bias and RMSE become −2.5% and 7.0%, respectively. Using a General Additive Model with coefficients depending on Sun zenith angle and aerosol optical thickness at 550 nm improves statistical performance in the absence of noise, especially in the ultraviolet, but provides similar performance on noisy data, indicating more sensitivity to noise. Adding spectral bands in the ultraviolet, e.g., centered on 325, 340, and 380 nm, yields marginally more accurate results in the ultraviolet, due to uncertainties in the gaseous transmittance. Comparisons between the measured and reconstructed Es spectra acquired by the MOBY spectroradiometer show agreement within predicted uncertainties, i.e., biases less than 2% in magnitude and RMS differences less than 5%. Reconstruction can also be achieved accurately with other sets of spectral bands and extended to cloudy conditions since cloud optical properties, like aerosol properties, tend to vary regularly with wavelength. These results indicate that it is sufficient, for many scientific applications involving hyper-spectral Es, to measure Es in a few coarse spectral bands in the ultraviolet to near infrared and reconstruct the hyperspectral signal using the proposed multivariate linear modeling.

A novel ocean profiling float system for calibrating and validating satellite-based ocean color observations has been developed and tested. The float-based radiometric sampling system, herein referred to as HyperNav, is complementary to traditional moored in-situ observing systems and provides additional capability due to the relatively small platform size and high radiometric accuracy that allows for opportunistic deployments at locations during seasons and conditions that are best for ocean color observations. The purpose of this study is to optimize the deployment locations of an array of HyperNav systems to support the Plankton, Aerosol, Cloud, ocean Ecosystem (PACE) mission by performing System Vicarious Calibration (SVC) observations. Specifically, we present the development of logistical and scientific criteria for selecting suitable sites for developing an SVC network of profiling-float-based radiometric systems capable of calibrating and validating ocean color observations. As part of the analyses described in this paper, we have synthetically deployed HyperNav at potential US-based and international sites, including: north of Crete island; south-east of Bermuda island; south of Puerto Rico island; southwest of Port Hueneme, CA; west of Monterey, CA; west of Kona, HI; and south-west of Tahiti island. The synthetic analyses identified Kona, Puerto Rico, Crete, and Tahiti as promising SVC sites. All sites considered are suitable for generating a significant number of validation match-ups. Optimally deploying HyperNav systems at these sites during the PACE post-launch SVC campaign is expected to cost-effectively provide a large number of SVC match-ups to fulfill the PACE calibration requirements.

In every liter of seawater there are between 10 and 100 billion life forms, mostly invisible, called marine plankton or marine microbiome, which form the largest and most dynamic ecosystem on our planet, at the heart of global ecological and economic processes. While physical and chemical parameters of planktonic ecosystems are fairly well measured and modeled at the planetary scale, biological data are still scarce due to the extreme cost and relative inflexibility of the classical vessels and instruments used to explore marine biodiversity. Here we introduce ‘Plankton Planet’, an initiative whose goal is to engage the curiosity and creativity of researchers, makers, and mariners to ( i ) co-develop a new generation of cost-effective (frugal) universal scientific instrumentation to measure the genetic and morphological diversity of marine microbiomes in context, ( ii ) organize their systematic deployment through coastal or open ocean communities of sea-users/farers, to generate uniform plankton data across global and long-term spatio-temporal scales, and ( iii ) setup tools to flow the data without embargo into public and explorable databases. As proof-of-concept, we show how 20 crews of sailors were able to sample plankton biomass from the world surface ocean in a single year, generating the first seatizen-based, planetary dataset of marine plankton biodiversity based on DNA barcodes. The quality of this dataset is comparable to that generated by Tara Oceans and is not biased by the multiplication of samplers. The data unveil significant genetic novelty and can be used to explore the taxonomic and ecological diversity of plankton at both regional and global scales. This pilot project paves the way for construction of a miniaturized, modular, evolvable, affordable and open-source citizen field-platform that will allow systematic assessment of the eco/morpho/genetic variation of aquatic ecosystems and microbiomes across the dimensions of the Earth system.

Phytoplankton play a major role on Earth, impacting the global distribution and cycles of carbon, oxygen, nitrogen, sulfur, and other elements, and structuring marine food webs. One fundamental trait of phytoplankton with direct biogeochemical implications is their size, as it governs metabolic and sinking rates as well as prey–predator interactions. Phytoplankton size spans approximately 3.5 orders of magnitude (when expressed as an equivalent spherical diameter), and thus measuring the full range in size distribution of phytoplankton is challenging and rarely attempted. Here, we constructed phytoplankton size spectra by merging state‐of‐the‐art cytometry and imaging cytometry measurements that were collected in the western North Atlantic Ocean, along a latitudinal gradient (36°N to 55°N) and during different phases of the annual cycle of phytoplankton. The derived spectra show a seasonal pattern that parallels changes in phytoplankton biomass, and do not always follow a commonly assumed power‐law model. Shifts in size spectra were more pronounced in the sub‐Arctic and temperate subregions, compared to the subtropical region of the study area. We evaluated the relationships between different size groups and environmental parameters to derive ecologically meaningful size groups. Finally, to simulate Ocean Color remote‐sensing algorithms of phytoplankton size, we compared temporal variations in descriptors of the size spectra (median particle size, phytoplankton size distribution exponent) with optical size proxies derived from light absorption and attenuation; good agreement was observed in the northern sections of the study area where temporal changes in community size structure were more pronounced.

Data recorded by autonomous solar tracking systems onboard ships can be used to validate remote-sensing reflectance observations collected by satellite sensors. Important water quality parameters can also be derived from these data using inverse models. These systems require a mechanical setup that is interfaced with a computer to log radiometric data and to optimally adjust radiometer direction relative to the sun and the platform on which the system is installed to avoid glint. Although solutions are known, no autonomous module compatible with the Sea-Bird Scientific HyperSAS radiometric system is currently available. Here, we present an open-source system, pySAS, that can be constructed for less than $5,000 (excluding radiometers). The pySAS uses two GPS units for orienting the radiometers to avoid effects of the platform's magnetic field. The system can be modified for use with other radiometers.

Estimating the biomass of phytoplankton communities via remote sensing is a key requirement for understanding global ocean ecosystems. Of particular interest is the carbon associated with diatoms given their unequivocal ecological and biogeochemical roles. Satellite-based algorithms often rely on accessory pigment proxies to define diatom biomass, despite a lack of validation against independent diatom biomass measurements. We used imaging-in-flow cytometry to quantify diatom carbon in the western North Atlantic, and compared results to those obtained from accessory pigment-based approximations. Based on this analysis, we offer a new empirical formula to estimate diatom carbon concentrations from chlorophyll a. Additionally, we developed a neural network model in which we integrated chlorophyll a and environmental information to estimate diatom carbon distributions in the western North Atlantic. The potential for improving satellite-based diatom carbon estimates by integrating environmental information into a model, compared to models that are based solely on chlorophyll a, is discussed.

The Tara Pacific expedition (2016-2018) sampled coral ecosystems around 32 islands in the Pacific Ocean, and sampled the surface of oceanic waters at 249 locations, resulting in the collection of nearly 58,000 samples (Gorsky et al. 2019, Planes et al. 2019, Flores et al. 2020). The expedition was designed to systematically study corals, fish, plankton, and seawater, and included the collection of samples for advanced biogeochemical, molecular, and imaging analysis. Here we provide the continuous dataset originating from the hyperspectral and multispectral spectrophotometers [ACS] instruments acquiring continuously during the full course of the campaign. Surface seawater was pumped continuously through a hull inlet located 1.5 m under the waterline using a membrane pump (10 LPM; Shurflo), circulated through a vortex debubbler, a flow meter, and distributed to a number of flow-through instruments. An [ACS] spectrophotometer (WETLabs) measured hyper-spectral (4 nm resolution) attenuation and absorption in the visible and near infrared except between Panama and Tahiti where an AC-9 multispectral spectrophotometer (WETLabs) was used instead. The flow was automatically directed through a 0.2 µm filter for 10 minutes every hour before being circulated through the spectrophotometer to eliminate the impact of biofouling and instrument drift and estimate particulate absorption [ap] and attenuation [cp] (Slade et al. 2010). Chlorophyll a content was estimated from particulate absorption line height at 676 nm (Boss et al. 2001). The particulate organic carbon concentration [poc] was estimated using an empirical relation (Gardner et al. 2006) between measured [poc] and measured [cp]. An indicator for size distribution of particles between 0.2 and ~20 µm [gamma] was calculated from [cp] (Boss et al 2001). The data was processed with custom software for underway optical data (InLineAnalysis software available on GitHub). The detailed information regarding the data processing is given in the processing report attached with the data and in Lombard et al. (In prep.). These results are preliminary: no matchup with in-situ chlorophyll from HPLC or [poc] measurements were performed.

The deep chlorophyll maximum (DCM) is a ubiquitous feature of phytoplankton vertical distribution in stratified waters that is relevant to our understanding of the mechanisms that underpin the variability in photoautotroph ecophysiology across environmental gradients and has implications for remote sensing of aquatic productivity. During the PEACETIME (Process studies at the air-sea interface after dust deposition in the Mediterranean Sea) cruise, carried out from 10 May to 11 June 2017, we obtained 23 concurrent vertical profiles of phytoplankton chlorophyll a, carbon biomass and primary production, as well as heterotrophic prokaryotic production, in the western and central Mediterranean basins. Our main aims were to quantify the relative role of photoacclimation and enhanced growth as underlying mechanisms of the DCM and to assess the trophic coupling between phytoplankton and heterotrophic prokaryotic production. We found that the DCM coincided with a maximum in both the biomass and primary production but not in the growth rate of phytoplankton, which averaged 0.3 d−1 and was relatively constant across the euphotic layer. Photoacclimation explained most of the increased chlorophyll a at the DCM, as the ratio of carbon to chlorophyll a (C:Chl a) decreased from ca. 90–100 (g:g) at the surface to 20–30 at the base of the euphotic layer, while phytoplankton carbon biomass increased from ca. 6 mg C m−3 at the surface to 10–15 mg C m−3 at the DCM. As a result of photoacclimation, there was an uncoupling between chlorophyll a-specific and carbon-specific productivity across the euphotic layer. The ratio of fucoxanthin to total chlorophyll a increased markedly with depth, suggesting an increased contribution of diatoms at the DCM. The increased biomass and carbon fixation at the base of the euphotic zone was associated with enhanced rates of heterotrophic prokaryotic activity, which also showed a surface peak linked with warmer temperatures. Considering the phytoplankton biomass and turnover rates measured at the DCM, nutrient diffusive fluxes across the nutricline were able to supply only a minor fraction of the photoautotroph nitrogen and phosphorus requirements. Thus the deep maxima in biomass and primary production were not fuelled by new nutrients but likely resulted from cell sinking from the upper layers in combination with the high photosynthetic efficiency of a diatom-rich, low-light acclimated community largely sustained by regenerated nutrients. Further studies with increased temporal and spatial resolution will be required to ascertain if the peaks of deep primary production associated with the DCM persist across the western and central Mediterranean Sea throughout the stratification season.

Sea spray aerosol (SSA) formation have a major role in the climate system, but measurements at a global-scale of this micro-scale process are highly challenging. We measured high-resolution temporal patterns of SSA number concentration over the Atlantic Ocean, Caribbean Sea, and the Pacific Ocean covering over 42,000 km. We discovered a ubiquitous 24-hour rhythm to the SSA number concentration, with concentrations increasing after sunrise, remaining higher during the day, and returning to predawn values after sunset. The presence of dominating continental aerosol transport can mask the SSA cycle. We did not find significant links between the diel cycle of SSA number concentration and diel variations of surface winds, atmospheric physical properties, radiation, pollution, nor oceanic physical properties. However, the daily mean sea surface temperature positively correlated with the magnitude of the day-to-nighttime increase in SSA concentration. Parallel diel patterns in particle sizes were also detected in near-surface waters attributed to variations in the size of particles smaller than ~1 µm. These variations may point to microbial day-to-night modulation of bubble-bursting dynamics as a possible cause of the SSA cycle.

One pathway by which the oceans influence climate is via the emission of sea spray that may subsequently influence cloud properties. Sea spray emissions are known to be dependent on atmospheric and oceanic physicochemical parameters, but the potential role of ocean biology on sea spray fluxes remains poorly characterized. Here we show a consistent significant relationship between seawater nanophytoplankton cell abundances and sea-spray derived Cloud Condensation Nuclei (CCN) number fluxes, generated using water from three different oceanic regions. This sensitivity of CCN number fluxes to ocean biology is currently unaccounted for in climate models yet our measurements indicate that it influences fluxes by more than one order of magnitude over the range of phytoplankton investigated.

The 14C incubation method for net primary production (NPP) has limited spatial/temporal resolution, while satellite approaches cannot provide direct information at depth. With chlorophyll-a and backscatter measurements from BGC-Argo floats, we quantified year-round NPP in the western North Atlantic Ocean using both the Carbon-based Productivity Model (CbPM) and Photoacclimation Productivity Model (PPM). Comparison with NPP profiles from 14C incubation measurements showed advantages and limitations of both models. CbPM reproduced the magnitude of NPP in most cases. However, in the summer the CbPM-based NPP had a large peak in the subsurface, which was an artifact from the subsurface chlorophyll maximum caused by photoacclimation. PPM avoided the artifacts from photoacclimation, but the magnitude of PPM-derived NPP was smaller than the 14C result. Different NPP distribution patterns along a North-South transect in the Western North Atlantic Ocean were observed, including higher winter NPP/lower summer NPP in the south, timing differences in NPP seasonal phenology, and different NPP depth distribution patterns in the summer months. Using a 6-months record of concurrent oxygen and bio-optical measurements from two Argo floats, we also demonstrated the ability of Argo floats to obtain estimates of the net community production to NPP ratio, ranging from 0.3 in July to -1.0 in December 2016. Our results highlight the utility of float bio-optical profiles and indicate that environmental conditions (e.g., light availability, nutrient supply) are major factors controlling the seasonality and spatial (horizontal and vertical) distributions of NPP in the western North Atlantic Ocean.

Raw data for "Seasonal mixed layer depth shapes phytoplankton physiology, viral production, and accumulation in the North Atlantic".

The goal of the EXport Processes in the Ocean from RemoTe Sensing (EXPORTS) field campaign is to develop a predictive understanding of the export, fate, and carbon cycle impacts of global ocean net primary production. To accomplish this goal, observations of export flux pathways, plankton community composition, food web processes, and optical, physical, and biogeochemical (BGC) properties are needed over a range of ecosystem states. Here we introduce the first EXPORTS field deployment to Ocean Station Papa in the Northeast Pacific Ocean during summer of 2018, providing context for other papers in this special collection. The experiment was conducted with two ships: a Process Ship, focused on ecological rates, BGC fluxes, temporal changes in food web, and BGC and optical properties, that followed an instrumented Lagrangian float; and a Survey Ship that sampled BGC and optical properties in spatial patterns around the Process Ship. An array of autonomous underwater assets provided measurements over a range of spatial and temporal scales, and partnering programs and remote sensing observations provided additional observational context. The oceanographic setting was typical of late-summer conditions at Ocean Station Papa: a shallow mixed layer, strong vertical and weak horizontal gradients in hydrographic properties, sluggish sub-inertial currents, elevated macronutrient concentrations and low phytoplankton abundances. Although nutrient concentrations were consistent with previous observations, mixed layer chlorophyll was lower than typically observed, resulting in a deeper euphotic zone. Analyses of surface layer temperature and salinity found three distinct surface water types, allowing for diagnosis of whether observed changes were spatial or temporal. The 2018 EXPORTS field deployment is among the most comprehensive biological pump studies ever conducted. A second deployment to the North Atlantic Ocean occurred in spring 2021, which will be followed by focused work on data synthesis and modeling using the entire EXPORTS data set.

The organic mass fraction from sea spray aerosol (SSA) is currently a subject of intense research. The majority of this research is dedicated to measurements in ambient air. However a number of studies have recently started to focus on nascent sea spray aerosol. This work presents measurements collected during a 5-week cruise in May and June 2017 in the central and western Mediterranean Sea, an oligotrophic marine region with low phytoplankton biomass. Surface seawater was continuously pumped into a bubble-bursting apparatus to generate nascent sea spray aerosol. Size distributions were measured with a differential mobility particle sizer (DMPS). Chemical characterization of the submicron aerosol was performed with a timeof-flight aerosol chemical speciation monitor (ToF-ACSM) operating with 10 min time resolution and with filter-based chemical analysis on a daily basis. Using positive matrix factorization analysis, the ToF-ACSM non-refractory organic matter (OMNR) was separated into four different organic aerosol types, identified as primary OA (POA(NR)), oxidized OA (OOA(NR)), methanesulfonic acid type OA (MSA-OA(NR)), and mixed OA (MOA(NR)). In parallel, surface seawater bio-geochemical properties were monitored providing information on phytoplankton cell abundance and seawater particulate organic carbon (1 h time resolution) and seawater surface microlayer (SML) dissolved organic carbon (DOC) (on a daily basis). Statistically robust correlations (for n > 500) were found between MOA(NR) and nanophytoplankton cell abundance, as well as between POA(NR), OOA(NR), and particulate organic carbon (POC). Parameterizations of the contributions of different types of organics to the submicron nascent sea spray aerosol are proposed as a function of the seawater biogeochemical properties for use in models.

Phytoplankton accessory pigments are commonly used to estimate phytoplankton size classes, particularly during development and validation of biogeochemical models and satellite ocean color-based algorithms. The diagnostic pigment analysis (DPA) is based on bulk measurements of pigment concentrations and relies on assumptions regarding the presence of specific pigments in different phytoplankton taxonomic groups. Three size classes are defined by the DPA: picoplankton, nanoplankton, and microplankton. Until now, the DPA has not been evaluated against an independent approach that provides phytoplankton size calculated on a per-cell basis. Automated quantitative cell imagery of microplankton and some nanoplankton, used in combination with conventional flow cytometry for enumeration of picoplankton and nanoplankton, provide a novel opportunity to perform an independent evaluation of the DPA. Here, we use a data set from the North Atlantic Ocean that encompasses all seasons and a wide range of chlorophyll concentrations (0.18-5.14 mg m(-3)). Results show that the DPA overestimates microplankton and picoplankton when compared to cytometry data, and subsequently underestimates the contribution of nanoplankton to total biomass. In contrast to the assumption made by the DPA that the microplankton size class is largely made up of diatoms and dinoflagellates, imaging-in-flow cytometry shows significant presence of diatoms and dinoflagellates in the nanoplankton size class. Additionally, chlorophyll bis commonly attributed solely to picoplankton by the DPA, but Chl b-containing phytoplankton are observed with imaging in both nanoplankton and microplankton size classes. We suggest revisions to the DPA equations and application of uncertainties when calculating size classes from diagnostic pigments.

During the North Atlantic Aerosols and Marine Ecosystems Study in the western North Atlantic, float‐based profiles of fluorescent dissolved organic matter and backscattering exhibited distinct spike layers at ∼ 300 m. The locations of the spikes were at depths similar or shallower to where a ship‐based scientific echo sounder identified layers of acoustic backscatter, an Underwater Vision Profiler detected elevated concentration of zooplankton, and mesopelagic fish were sampled by a mesopelagic net tow. The collocation of spike layers in bio‐optical properties with mesopelagic organisms suggests that some can be detected with float‐based bio‐optical sensors. This opens the door to the investigation of such aggregations/layers in observations collected by the global biogeochemical‐Argo array allowing the detection of mesopelagic organisms in remote locations of the open ocean under‐sampled by traditional methods.