Proteomic characterization of plasma is critical for the development of novel pharmacodynamic biomarkers. However, the vast dynamic range renders the profiling of proteomes extremely challenging. Here, we synthesized zeolite NaY and developed a simple and rapid method to achieve comprehensive and deep profiling of the plasma proteome using the plasma protein corona formed on zeolite NaY. Specifically, zeolite NaY and plasma were co-incubated to form plasma protein corona on zeolite NaY (NaY-PPC), followed by conventional protein identification using liquid chromatography-tandem mass spectrometry. NaY was able to significantly enhance the detection of low-abundance plasma proteins, minimizing the "masking" effect caused by high-abundance proteins. The relative abundance of middle- and low-abundance proteins increased substantially from 2.54% to 54.41%, and the top 20 high-abundance proteins decreased from 83.63% to 25.77%. Notably, our method can quantify approximately 4000 plasma proteins with sensitivity up to pg/mL, compared to only about 600 proteins identified from untreated plasma samples. A pilot study based on plasma samples from 30 lung adenocarcinoma patients and 15 healthy subjects demonstrated that our method could successfully distinguish between healthy and disease states. In summary, this work provides an advantageous tool for the exploration of plasma proteomics and its translational applications.

Achieving oriented and ordered remineralization on the surface of demineralized dental enamel, thereby restoring the satisfactory mechanical properties approaching those of sound enamel, is still a challenge for dentists. To mimic the natural biomineralization approach for enamel remineralization, the biological process of enamel development proteins, such as amelogenin, was simulated in this study. In this work, carboxymethyl chitosan (CMC) conjugated with alendronate (ALN) was applied to stabilize amorphous calcium phosphate (ACP) to form CMC/ACP nanoparticles. Sodium hypochlorite (NaClO) functioned as the protease which decompose amelogenin in vivo to degrade the CMC-ALN matrix and generate HAP@ACP core-shell nanoparticles. Finally, when guided by 10 mM glycine (Gly), HAP@ACP nanoparticles can arrange orderly and subsequently transform from an amorphous phase to well-ordered rod-like apatite crystals to achieve oriented and ordered biomimetic remineralization on acid-etched enamel surfaces. This biomimetic remineralization process is achieved through the oriented attachment (OA) of nanoparticles based on non-classical crystallization theory. These results indicate that finding and developing analogues of natural proteins such as amelogenin involved in the biomineralization by natural macromolecular polymers and imitating the process of biomineralization would be an effective strategy for enamel remineralization. Furthermore, this method represents a promising method for the management of early caries in minimal invasive dentistry (MID).

Polylactic acid(PLA)nonwoven materials were treated by low temperature plasma under different treatment time.Firstly,the weight loss rate of materials was calculated before and after treatment.And to observe the surface properties and internal structures of samples,scanning electron microscopy(SEM) and Fourier transform infrared spectroscopy(FT-IR) were used.Finally,the study focused on the wetting contact angle and the biodegradable property of materials,which were modified by low temperature plasma.The results indicate that the weight loss rate of PLA nonwoven materials increases significantly with the increase of treatment time.The wetting contact angle of samples declines from 113.86° to 70.27° when they are treated for only 5 min,which is due to the etching effect of plasma and some functional groups especial hydrophilic groups added by plasma treatment.Ultimately,hygroscopicity and biodegradability of materials are improved.

The characteristics of host-guest complexes between cucurbit[n]uril (CB [n]) and phenylalanine were investigated by UV-visible absorption spectroscopy in acetate buffer solution at room temperature. It was found that the UV-visible absorption increased steadily with constantly dropping the high concentration of cucurbit[6]uril (CB [6]) and cucurbit[8]uril (CB [8]) in the phenylalanine solution which indicates that there are some interaction betweenCB [n] and phenylalanine.Then CB [6] and phenylalanine at molar ratio of 1:1 to weigh while CB [8] and phenylalanine at molar ratio of 1:2, respectively, are both demonstrated by 1H NMR spectra. 1H NMR spectrum of complexes was obtained, indicating an enthalpic driving force for host-guest complexes. The possible interaction mechanism and inclusion mode were also discussed. This work may extend the application range of CB [n] in supramolecular and pharmaceutical analysis.

The study focused on novel surface modification technology: low temperature plasma, which is characterized by clean, high efficiency and energy-saving. Polylactic acid (PLA) non-woven materials were treated by this means under different treatment time. The SEM and FT-IR were used to observe the surface properties and internal structures of samples. Whether the wetting contact angle and the biodegradable property of materials were modified by low temperature plasma was discussed. The results indicated that with the increase of treatment time, the etching effect of plasma and some functional groups especial hydrophilic groups increased significantly. When they were only treated 3 minutes the wetting contact angle declined from 113.86° to 85.49°. Additionally, the hygroscopicity and biodegradability of materials were improved by low temperature plasma treatment.

Cucurbit[n]uril as one of the supramolecular complexes have many potential values because of the unique structure and properties. In this paper, the characteristics of host-guest complexes between cucurbit[n]uril and the reactive brilliant red X-3B were investigated by UV - visible absorption spectroscopy in acetate buffer solution at room temperature. It was found that the UV - visible absorption increased steadily with dropping the high concentration of cucurbit [6] uril (CB [6]) and cucurbit [8] uril (CB [8]) in the reactive brilliant red X-3B solution which indicates that that CB [8] has adsorption to reactive brilliant red X-3B dye and CB [8] can form host-guest complexes with reactive brilliant red X-3B dye. This work may extend the range of the application cucurbit [n] unit in the textile industry and pollution water treatment.