Feline coronavirus (FCoV), together with an inadequate host immune response, causes feline infectious peritonitis (FIP), one of the most fatal infectious diseases in cats worldwide. This study investigated, for the first time in Konya, T & uuml;rkiye, the presence and phylogenetic relationships of FCoV Types I and II in cats. Fecal and effusion samples were collected from 30 cats suspected of having FIP. Viral RNA was extracted, and FCoV-I/II genomes were detected using nested reverse transcriptase-polymerase chain reaction (RT-PCR). Six positive samples were sequenced for phylogenetic and amino acid analyses, and results were statistically evaluated. Nested PCR results showed that 30% (9/30) of effusion samples were positive for FCoV-I, 13.33% (4/30) for FCoV-II, and 6.66% (2/30) were positive for both FCoV types. Among fecal samples, 33.33% (10/30) were positive for FCoV-I, while no FCoV-II was detected. No statistically significant association was found between FCoV positivity and factors such as gender or age. This study demonstrates, for the first time at the molecular level, the coexistence of FCoV-I and FCoV-II strains in T & uuml;rkiye and their genetic similarity with global variants. Evaluating fecal and effusion samples together enhances diagnostic accuracy and enables the use of alternative specimens in FIP diagnosis. These findings are crucial for understanding FCoV evolution, controlling disease spread, and developing effective therapeutic and preventive strategies. Moreover, this study provides the first comprehensive phylogenetic characterization of FCoV Types I and II in T & uuml;rkiye, contributing valuable data to global feline coronavirus epidemiology and vaccine research.

Canine Parvovirus (CPV) is a highly contagious viral disease that affects domestic and wild dogs worldwide, causing high morbidity and mortality, especially in puppies. Early and accurate diagnosis of the virus is crucial for reducing secondary infections and preventing the spread of the disease among susceptible dogs. In this study, the presence of CPV infection was detected in fecal samples collected from unvaccinated dogs aged 0–12 months using Enzyme-Linked ImmunoSorbent Assay (ELISA) and a rapid test kit (immunochromatographic (IC)), and the results of these common diagnostic methods were compared. Additionally, CPV IgG antibody seropositivity was investigated from blood serum samples to evaluate protective titers. Among 100 dogs (51% female, 49% male), 49 (49%) tested positive for CPV-2 Ag via the IC test kit, while 17 (17%) were positive using the ELISA Ag test. Compared to the rapid test, the ELISA test detected CPV at a lower rate in puppies, suggesting that it may be preferred as a specific and sensitive test for identifying CPV in unvaccinated dogs. Moreover, among 42 dogs considered antibody-positive based on ELISA kit cut-off values, 22 (52.4%) had high CPV IgG antibody titers, while 20 (47.6%) had low antibody titers. Statistical analyses showed no significant difference between gender and immunity levels (P > 0.001). However, a significant relationship was observed between the age of dogs and immunity levels (P < 0.001). The study confirmed the presence of CPV-specific IgG antibodies in dogs from the Alanya. In conclusion, this study has provided readers with information on the prevalence of CPV infection, test reliability, and seroprevalence in Alanya region, recommending the development of strategies for disease control.

Bovine enterovirus (BEV) infection is a common viral disease in cattle. Although the infection is often subclinical, it is among the possible causes of gastroenteritis. BEV infections have also been associated with respiratory and reproductive system disorders and signs of diarrhea. BEV is divided into two serotypes, enterovirus serotype E (EV-E) and enterovirus serotype F (EV-F). This study aims to determine the seroprevalence of EV-E in calves in Konya province. For this purpose, 504 BEV unvaccinated calf blood serum samples from the Selcuk University Veterinary Faculty Virology Department laboratory were used. A serum neutralization test (SNT) was used to determine EV-E seroprevalence. 342 (67.85%) samples were found to be seropositive, and 162 (32.14%) were seronegative. In addition, as the serum neutralization 50 (SN50) antibody titers of seropositive animals were examined, the titers determined as 1/10, 1/15 and 1/20. Among the results we obtained, the highest antibody titers were defined as 1/80 and 1/120; the total number of animals with these values was In conclusion, bovine enteroviruses are an infection of importance for cattle breeding. Therefore, necessary precautions must be taken to protection infection. It is thought that the results of the present study will provide important data for future studies.

Background: Bovine Herpesvirus 1 (BoHV-1) is an important bovine pathogen found throughout the world. It causes a subclinical, mild, or severe disease. It has different types of clinical manifestations in respiratory, genital, ocular, and encephalomyelitis forms. The aim of this study was to perform comparative diagnosis of BoHV-1 by Real Time PCR and Real Time LAMP and molecular characterization of BoHV-1. Materials, Methods & Results: In this study, BoHV-1 was investigated in nasal swab samples from cattle with clinical signs of respiratory problems using Real-Time PCR (Polymerase Chain Reaction) methods and the Real-Time Loop-Mediated Isothermal Amplification (LAMP) method, which is a cheap, fast, and reliable technique. BoHV-1 positive samples in both tests were cultured in the Madin-Darby Bovine Kidney (MDBK) cell culture. Conventional PCR was performed for the molecular characterization of the isolated samples. Eight (5.33%) nasal swab samples used in the study were found to be positive by the Real-Time PCR test, while 13 (8.66%) samples were found to be positive by the LAMP test. The results of the McNemar test showed that the difference between Real-Time PCR and Real-Time LAMP tests was not significant (P. 0.05). BoHV-1 was isolated from 3 of 8 samples cultured in cell culture. In the sequence analysis of these three samples after PCR, molecular characterization of the isolates was performed, and they were found to belong to the BoHV1.1 subtype. These findings indicated that the LAMP method can be used in the rapid diagnosis of BoHV-1 infection, and the BoHV-1.1 subtype should be considered in the fight against infection. Discussion: Many studies have shown that PCR tests are more sensitive than virus isolation (VI). Similarly, in this study, only 3 of the 8 samples found to be positive by Real-Time PCR could be isolated in cell culture. The results of both tests differed in that PCR can detect any viral RNA (infectious whole virus, unpackaged viral genome, even genome strand or subgenomic fragments) whereas VI detects properly packaged and live virus particles. Although no statistical difference was recorded between Real-Time PCR and Real-Time LAMP in this study, a higher rate of positive samples was detected with LAMP, which matched the findings of other reports. These results showed that LAMP is a more resistant to PCR inhibitors and more sensitive test and due to the use of 6 different primers. BoHV-1 continues to cause significant economic losses in the cattle industry around the world. Therefore, a practical, fast, and cost-effective diagnostic tool needs to be used to identify the infection in the affected areas and control it at the earliest. In this context, the LAMP test is a promising method at the field level for the diagnosis and control of BoHV-1 infection and other diseases, as mentioned in other studies. Phylogenetic analysis was performed on 3 swab samples isolated in cell culture, after they were tested by conventional PCR with primers specific to the BoHV-1 gC region, in parallel with other reports. The isolate sequence was aligned using MUSCLE, with the sequences of other isolates obtained by a comparative analysis and multiple sequence alignment was performed in the AliView software. This analysis included the nucleotide sequences of 54 different isolates. All ambiguous locations were removed for each sequence. There were 312 nucleotides in the final dataset. Evolutionary analysis was performed using the Tamura 3-parameter model with the Neighbor-Joining algorithm in MEGA 11; in total, 1,000 iterations (bootstrap) were performed. Similar to the findings of other studies conducted in Turkey, it was determined in this study that the more common BoHV-1 strain in circulation was the BoHV-1.1 strain.

Hoof diseases are very common causes of lameness and hoof health, and it is very important factor for productivity and yield capacity in the small ruminants. This study was conducted in 97 Saanen goats, 12-18 monthsold, and weighing 30-40 kg. Hoof overgrowth (HO), white line disease (WLD), lameness score (LS), and body conditions core (BCS) were scored using 3 -point, 5 -point, 4 -point and 5 -point scale systems, respectively. The hooves of all goats were evaluated for foot rot and interdigital dermatitis, toe granuloma/abscess and laminitis as absent or present and not found in any of the goats included in the study. A negative and significant relationships were found between the BCS and LS, HO and WLD scores. Mean HO was significantly lower only for the right fore claw, but there was statistically significant difference between all the four claws for WLD. Also, positive and highly significant relationship was found between HO and WLD. The incidence of HO between the fore and hind claws was not significantly different, but WLD in the hind claw was significantly higher than the fore claws. Consequently, in goats, lower BCS was associated with increase in the severity of LS, HO and WLD. Also, at a higher rate WLD was seen in the hind legs and, it has been demonstrated that HO may predispose to WLD.

Three-day sickness, also known as Bovine Ephemeral Fever (BEF), is an arboviral-derived disease of cattle and water buffaloes progressing with acute, rapidly developing polyphasic fever and showing symptoms such as stiffness of muscle tissue and excessive salivation. While clinical findings generally continue between 1-3 days, it has importance due to a sudden and high decrease in milk yield in cows during lactation, infertility, loss of condition, treatment costs and sometimes serious economic losses due to death of sick animals. The possible spread mechanism is reported to be related to the inter-regional wind movement of Culicoides-type sandflies and various insect vectors and host feeding of these blood-fed vectors. The objective of this review is to provide information about the current situation of the three-day sickness in the light of current epidemiological data and to draw a perspective for the future.

Aim: This study was designed to identify the tick species infesting cattle in the southern region of Konya and to investigate the presence of Crimean-Congo Hemorrhagic Fever (CCHF) in these ticks. Materials and Methods: Tick samples were obtained from 60 cattle selected from 30 farms, where tick infestations were macroscopically observed in the livestock. The presence of CCHF was investigated molecularly by RT-PCR method. Ticks infesting 60 cattle from 30 farms located in the five designated study areas (Karabayir, Karacahisar, Arslantas, Kozagac, and Kayapinar neighborhoods) were collected. The collected ticks were identified to species. Results: A total of 117 ticks were identified, with 111 (94.87%) identified as Hyalomma marginatum, 3 (2.56%) identified as Hyalomma excavatum, and 3 (2.56%) identified as Dermacentor marginatus. In the RT-PCR analysis conducted on 35 pools of ticks (considering tick species, sex, and collection area), no presence of the CCHF virus was detected. Conclusion: The identification of the majority of the ticks in this study as Hyalomma marginatum is noteworthy. The identification of Hyalomma marginatum as the vector of the CCHF disease in Turkiye is indicative of increasing concerns. Molecular and serological evidence showing the presence of the CCHF virus in the region, albeit limited, indicates that more comprehensive studies related to the disease are necessary.

This study was conducted to determine and evaluate the BoLA-DRB3.2 polymorphisms in bovine leukemia virus (BLV) seropositive cows by DNA Sequence Analysis. Isolates of DNA obtained from seropositive (25) and seronegative blood samples (3) collected from cows between 2005 and 2010 were used in this study. DNA isolation was performed from blood samples. MHC analyses were performed using primers belonging to BoLA-DRB3.2 genes. DNA sequence analyses were performed. The number and diversity of haplotypes and nucleotide diversity were calculated. The phylogenetic analysis was determined that cows had 11 haplotypes, healthy individuals were associated together in the first haplotype, while in other haplotypes seropositive cows were associated among themselves and there was 1 seropositive cow between haplotype 6 and 11. Also, the variety of haplotypes (Hd) (0.847), total nucleotide variety (pi) (0.033), variation rate (0.00285), and Tajima's D statistical value (0.103) were detected. When FST of individuals were evaluated, it was revealed that healthy individuals were genetically distant from positive individuals. In this study, it was observed that BLV seropositive individuals differed genetically compared to healthy animals. In conclusion, BoLA-DRB3.2 gene polymorphism can be considered as a supporting parameter in BLV diagnosis.

Foot-and-mouth disease (FMD) and bluetongue disease (BT) are two of the most devastating viral diseases affecting domestic and wild ruminants. They cause significant economic losses, and vaccination is the most important method for combating both FMD and BT. Simultaneous vaccinations are crucial for animal welfare as well as labor and cost savings. No reports were found on the simultaneous administration of FMD and BT vaccines to sheep. This study evaluated the effect of the simultaneous administration of an inactivated FMD vaccine and an attenuated BT vaccine on the humoral immune response to both diseases. A total of 75 sixmonth- old Akkaraman-Kıvırcık crossbred sheep that did not have any vaccination history were used as research material. Before the experiment, the animals were tested for the absence of FMD and BT antibodies. The sheep were divided into three experimental groups and vaccinated with an FMD vaccine alone, a BT vaccine alone, and both. Blood samples were taken on days 30 and 60 post-vaccination. The blood samples were evaluated using liquid-phase blocking ELISA (LPBE) and virus neutralization test (VNT) for FMD antibodies, as well as competitive ELISA (c-ELISA) and VNT for BT antibodies. The study revealed that differences between the FMD and BT antibody titers of the group vaccinated simultaneously and the FMD and BT antibody titers obtained from the groups vaccinated separately were not statistically significant (p > 0.05). It was concluded that the antibody response these diseases was not altered when the FMD and BT vaccines were administered together.

Aim: This research was carried out to detect CPV and CCoV infections in dogs in comparison with rapid kit and PCR and to determine the molecular characterization of these infections in Konya region. Besides, it was aimed to determine the sensitivity and specificity rates of the diagnostic tests after fresh or freeze-thawed stool for infection diagnosis. Materials and Methods: Faecal samples were collected from 50 unvaccinated, 0-12 months old dogs with diarrhoea symptoms at the shelter. The samples were analysed for CPV and CCoV by rapid test and PCR test. After freezethawing, the samples were checked again with the same tests. Results: CPV was positively diagnosed by rapid test and PCR in 2 and 29 fresh stool samples, respectively, and CCoV in 14 and 28 samples. CPV positive samples did not change while CCoV was diagnosed as positive in 10 samples and 28 samples by rapid test and PCR, respectively, after the freeze-thaw procedure. Although there were no differences in the diagnosis of CPV, the sensitivity of the rapid test in the diagnosis of CCoV decreased after the freezethaw procedure. In addition, only CPV-2b type was detected in CPV positive samples and both GI and GII subtypes were detected in CCoV positive samples as molecular. In conclusion, it was observed that rapid tests are not sensitive for accurate diagnosis of CPV and CCoV infections. Conclusion: The importance of choosing molecular diagnostic methods and using fresh samples for accurate diagnosis of virological infections can be emphasized.

The transmission of West Nile Virus (WNV) to new locations is mostly facilitated by migratory birds. Türkiye's domestic ducks, geese, and chickens have already tested positive for WNV by serology. This study was conducted to identify the seroprevalence of WNV in wild bird species because wild birds in the Western Mediterranean Region are found along migration routes from Africa to Europe, they are home to a wide variety of bird species due to the abundance of lakes and wetlands, the mild Mediterranean climate, and some areas that are suitable habitat for mosquitoes due to their low altitude. Serum samples were taken from 141 wild birds in Isparta (66), Burdur (42), and Antalya (33) for this study on birds in the wild. During serological studies, there was no evidence of WNV-specific antibodies.

aim of the present study was to compare the electrocardiographic findings, heart rate, cloacal tem-perature, respiratory rate, and anaesthetic effects of medetomidine-ketamine to detomidine-ketamine in buzzards. Fourteen buzzards of unknown sex and age and weighing a mean +/- standart deviation (SD) of 789.29 +/- 82.97 g were included in the study. The buzzards were randomly assigned to two groups: MK group (0.1 mg kg-1 medetomidine and 10 mg kg-1 ketamine 10 minutes after medetomidine application) and group DK (0.3 mg kg-1 detomidine and 10 mg kg-1 ketamine 10 minutes after medetomidine application). Heart rates (HR), respiratory rates (fR), and cloacal temperatures (CT) were recorded before administration of any drug (0) and then 5 and 10 minutes after medetomidine and 5, 10, 15, 30, 45, 60 minutes after ketamine. At this point, atipamezole (0.5 mg/kg, IM) was administered and measurement recorded at 5, 10 and 15 minutes. Serum electrolyte and blood gases were measured before and during anesthesia. Elec-trocardiogram was recorded before and during anesthesia. Distribution and suitability of the data were evaluated by the Shapiro-Wilks test. Statistical tests were performed via two-way variance analysis in repeated measures, and multiple comparisons were corrected with the post-hoc Generalized Linear Model (GLM). Differences were considered to be statistically significant if p < 0.05. Onset of anesthesia time was 4.1 +/- 2.3 and 3.9 +/- 1.3 minutes (mean +/- standard deviation) in groups MK and DK, respectively. Reflexes (righting reflex, toe pinch reflex, feather plucking reflex, palpebral reflex) disappeared studied in both groups during anesthesia. HR,fR , and CT were significantly decreased in both groups (p < 0.001). Baseline blood gases values showed significantly increases in pCO2 and HCO3 and decreases in pH and pO2 during anesthesia in both groups (p < 0.05). In conclusions medetomidine-ketamine and detomidine-ketamine combinations were both sufficient to provide desired level of anesthesia, analgesia and muscle relaxation.

This study was carried out to investigate the presence of BNoV in cattle and its phylogenetic analysis. The fecal samples from a total of 80 cattle (ages 2 to 5 years old) brought to a slaughterhouse in Konya for slaughter (between 2019-2020) were collected, regardless of clinical symptoms and gender, and examined for the presence of nucleic acid by RT-PCR. Six samples out of the tested fecal samples (7.5%) were found to be positive for the presence of BNoV nucleic acid. DNA sequences were analyzed by comparing the nucleotide gene sequences specific to BNoV isolates with GIII genotype and the RdRp gene region of the virus in different regions of Turkey and the world. As a result of the phylogenetic analysis in line with the data obtained, it was determined that the virus genotype circulating in the sampling region was included in the GIII.2 cluster-2. It can be concluded that the BnoV infection exists in the country, therefore, it can be stated that protection and control measures should be applied within the scope of the struggle.

Foot-and-mouth disease (FMD) and bluetongue disease (BT) are two of the most devastating viral diseases affecting domestic and wild ruminants. They cause significant economic losses, and vaccination is the most important method for combating both FMD and BT. Simultaneous vaccinations are crucial for animal welfare as well as labor and cost savings. No reports were found on the simultaneous administration of FMD and BT vaccines to sheep. This study evaluated the effect of the simultaneous administration of an inactivated FMD vaccine and an attenuated BT vaccine on the humoral immune response to both diseases. A total of 75 sixmonth-old Akkaraman-Kivircik crossbred sheep that did not have any vaccination history were used as research material. Before the experiment, the animals were tested for the absence of FMD and BT antibodies. The sheep were divided into three experimental groups and vaccinated with an FMD vaccine alone, a BT vaccine alone, and both. Blood samples were taken on days 30 and 60 post-vaccination. The blood samples were evaluated using liquid-phase blocking ELISA (LPBE) and virus neutralization test (VNT) for FMD antibodies, as well as competitive ELISA (c-ELISA) and VNT for BT antibodies. The study revealed that differences between the FMD and BT antibody titers of the group vaccinated simultaneously and the FMD and BT antibody titers obtained from the groups vaccinated separately were not statistically significant (p > 0.05). It was concluded that the antibody response these diseases was not altered when the FMD and BT vaccines were administered together.

Bovine enterovirus (BEV) infection is a common viral disease in cattle. Although the infection is often subclinical, it is among the possible causes of gastroenteritis. BEV infections have also been associated with respiratory and reproductive system disorders and signs of diarrhea. BEV is divided into two serotypes, enterovirus serotype E (EV-E) and enterovirus serotype F (EV-F). This study aims to determine the seroprevalence of EV-E in calves in Konya province. For this purpose, 504 BEV unvaccinated calf blood serum samples from the Selcuk University Veterinary Faculty Virology Department laboratory were used. A serum neutralization test (SNT) was used to determine EV-E seroprevalence. 342 (67.85%) samples were found to be seropositive, and 162 (32.14%) were seronegative. In addition, as the serum neutralization 50 (SN50) antibody titers of seropositive animals were examined, the titers determined as 1/10, 1/15 and 1/20. Among the results we obtained, the highest antibody titers were defined as 1/80 and 1/120; the total number of animals with these values was In conclusion, bovine enteroviruses are an infection of importance for cattle breeding. Therefore, necessary precautions must be taken to protection infection. It is thought that the results of the present study will provide important data for future studies.

ABSTRACT: Cats are susceptible to feline panleukopenia virus (FPV) and canine parvovirus type 2 (CPV-2). Therefore, coinfection and superinfection with multiple parvovirus strains may occur, resulting in high heterogeneity and recombination. Considering the importance of cats as a potential source of genetic diversity for parvoviruses, we investigated the frequency of parvovirus infection in cats using their blood and fecal samples and performed molecular characterization of parvovirus strains circulating in cat populations. Accordingly, the fecal and blood samples of 60 cats with gastroenteritis symptoms were collected from Turkey’s Burdur, Isparta, and Izmit provinces. Of these 15 fecal samples tested as parvovirus-positive by PCR, 14 were confirmed to have been infected with true FPV strains by sequencing analysis. Through the phylogeny analysis, those were located in the FPV cluster, closely related to CPV-2, and one was discriminated in the CPV-2b cluster. Additionally, sequence analysis of the VP2 gene of CPV and FPV revealed that the FPV strains detected in Turkey and the vaccine strains were highly related to each other, with a nucleotide identity of 97.7- 100%. Furthermore, 13 variable positions were detected in VP2 of the field and reference FPV strains. Three synonymous mutations were determined in the VP2 gene. Some amino acid mutations in the VP2 protein-affected sites were considered responsible for the virus’s biological and antigenic properties. The partial sequence analysis of the VP2 gene revealed that four FPV strains detected in Turkey have a single nucleotide change from T to G at the amino acid position 384 between the nucleotides 3939-3941, which was reported for the first time. Therefore, these four isolates formed a different branch in the phylogenetic tree. The results suggest that both FPV and CPV-2b strains are circulating in domestic cats in Turkey and cats should be considered as potential sources of new parvovirus variants for cats, dogs and other animals.

mThe aim of this study was to investigate determination of antioxidant capacity and changes of biochemical parameters [Albumin, ALP (Alkaline phosphatase), ALT (Alanin transaminase), AST (Aspartate aminotransferase), cholesterol, CK-MB (Creatine kinase), GGT (Gama-glutamil transferase), T-Protein, Triglycerine, BUN (Blood Urea Nitrogen)] on calves persistently infected (PI) with Bovine viral diarrhea virus (BVDV) in Konya. Blood samples (both sera and leukocyte) will be taken from the PI calves. Obtained samples will be screened for BVDV antigen and antibodies, antioxidant capacity and changes of biochemical parameters with the use of a commercially available Enzyme Linked Immunosorbent Assay (ELISA) kits. Independent T test will be performed for comparing results between PI calves and a control group. P<0.05 value will be considered significance level. A statistically significant difference was found between ALB, ALP, Cholesterol, CK-MB, TP values (P <0.05) while there was no statistically significant difference MDA and TAC values between control and study group (p>0.05). It can be communicable that persistently BVDV infection has been damage the heart, duodenum and liver, although affecting lipid metabolism and lipid peroxidation. In conclusion the fact that the PI animals to be detected are to be removed from farms is of economic importance both our province and others. In addition, changes in biochemical parameters and antioxidant capacities in persistent infected animals will give some information about the pathogenesis of the infection.

ABSTRACT: Goose parvovirus (GPV), also called Derzsy’s disease, is a viral pathogen that causes high morbidity and mortality in goslings and ducklings. In this study, we perform the molecular characterization of the GPV in Turkey. The definition of similarity to the world of GPV isolates in Turkey and construction of a phylogenetic tree was aimed. For this purpose, the presence of GPV in the liver, spleen, and intestine tissues of nine goslings with symptoms such as dysphagia, bilateral ocular swelling, eye discharge, diarrhea, and fatigue were investigated by real-time PCR method and all samples were detected as positive. According to the data obtained by molecular characterization, phylogenetic analysis of GPV has been presented in Turkey. As a result of this study, it was determined that the GPVs available in Turkey are virulent strains.

Aim: Coronaviruses and Rotaviruses are important virologic factors for both animal and human health in Turkey and the world. Bovine Rotavirus (BRV) and Bovine Coronavirus (BCoV) in cattle cause significant economic losses. The aim of this study was to determine the presence of BRV and BCoV in Anatolian buffaloes which were on the same farms with cattle. For this purpose, presence of these two viruses were investigated by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and BRV-BCoV Rapid tests and sensitivity and specificity ratios of these two tests were compared. Materials and Methods: In this study, 230 Anatolian buffaloes were clinically evaluated in cattle farms in Afyonkarahisar region. Fecal samples were collected from 27 buffaloes which had clinical signs (weakness, dehydration, vomiting, watery consistency and yellow stool). The fecal samples were evaluated by Rapid Test and RT-PCR for Bovine Rotavirus and Bovine Coronavirus. The analyzes were performed according to the procedure of the commercial RT-PCR and rapid kits. Results: The RT-PCR results were positive as 22.2% (6/27) for BRV and 3.7% (1/27 27/1) for BcoV while Rota-Corona Rapid test results were negative in all samples. When compared with RT-PCR results for both viruses, the rapid test sensitivity and specificity was determined as 0% and 100%, respectively. In addition, positive rates of BRV was statistically important as BCoV rate in analyzed samples (p<0,05). Conclusion: In conclusion, low sensitivity of rapid test may be due to the change in the amount of virus scattered throughout the course of enteric infections.