Protein-carbohydrate interactions are very often mediated by the stacking CH-π interactions involving the side chains of aromatic amino acids such as tryptophan (Trp), tyrosine (Tyr) or phenylalanine (Phe). Especially suitable for stacking is the Trp residue. Analysis of the PDB database shows Trp stacking for 265 carbohydrate or carbohydrate like ligands in 5 208 Trp containing motives. An appropriate model system to study such an interaction is the AAL lectin family where the stacking interactions play a crucial role and are thought to be a driving force for carbohydrate binding. In this study we present data showing a novel finding in the stacking interaction of the AAL Trp side chain with the carbohydrate. High resolution X-ray structure of the AAL lectin from Aleuria aurantia with α-methyl-l-fucoside ligand shows two possible Trp side chain conformations with the same occupation in electron density. The in silico data shows that the conformation of the Trp side chain does not influence the interaction energy despite the fact that each conformation creates interactions with different carbohydrate CH groups. Moreover, the PDB data search shows that the conformations are almost equally distributed across all Trp-carbohydrate complexes, which would suggest no substantial preference for one conformation over another.

The Aleuria aurantia lectin (AAL) derived from orange peel fungus contains five fucose-binding sites that recognizes fucose bound in α-1,2, α-1,3, α-1,4, and α-1,6 linkages to N-acetylglucosamine and galactose. Recently, we have created several recombinant AAL (rAAL) proteins that had altered binding affinity to fucose linkages. In this report, we further characterize the binding specificity of one of the mutated lectins, N224Q lectin. This lectin was characterized by lectin Western blotting, surface plasmon resonance, and glycan microarray and shown to have increased binding to fucosylated glycan. Subsequently, we used this lectin to identify secreted fucosylated glycoproteins from a fetal hepatic cell line. Proteomic analysis revealed several glycoproteins secreted by the fetal cell line that were bound by N224Q lectin. These findings were confirmed by subsequent proteomic analysis of human serum from control patients or patients with hepatocellular carcinoma. These represent candidate oncofetal markers for liver cancer.

Nanosatellites are spacecraft in the mass range between 1 and 10 kg providing a fast and low-cost possibility to test new technology in Space and gain flight heritage. The first Austrian nanosatellite TUGSAT-1/BRITE-Austria, successfully launched in 2013, and a follow-up ESA mission are described in this paper.

A new matrix assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) method to spatially profile the location and distribution of multiple N-linked glycan species in tissues is described. Application of an endoglycosidase, peptide N-glycosidase F (PNGaseF), directly on tissues followed by incubation releases N-linked glycan species amenable to detection by MALDI-IMS. The method has been designed to simultaneously profile the multiple glycan species released from intracellular organelle and cell surface glycoproteins, while maintaining histopathology compatible preparation workflows. A recombinant PNGaseF enzyme was sprayed uniformly across mouse brain tissue slides, incubated for 2 h, then sprayed with 2,5-dihydroxybenzoic acid matrix for MALDI-IMS analysis. Using this basic approach, global snapshots of major cellular N-linked glycoforms were detected, including their tissue localization and distribution, structure, and relative abundance. Off-tissue extraction and modification of glycans from similarly processed tissues and further mass spectrometry or HPLC analysis was done to assign structural designations. MALDI-IMS has primarily been utilized to spatially profile proteins, lipids, drug, and small molecule metabolites in tissues, but it has not been previously applied to N-linked glycan analysis. The translatable MALDI-IMS glycan profiling workflow described herein can readily be applied to any tissue type of interest. From a clinical diagnostics perspective, the ability to differentially profile N-glycans and correlate their molecular expression to histopathological changes can offer new approaches to identifying novel disease related targets for biomarker and therapeutic applications.

We propose a new satellite mission to deliver high quality measurements of upper air water vapour. The concept centres around a LiDAR in limb sounding by occultation geometry, designed to operate as a very long path system for differential absorption measurements. We present a preliminary performance analysis with a system sized to send 75 mJ pulses at 25 Hz at four wavelengths close to 935 nm, to up to 5 microsatellites in a counter-rotating orbit, carrying retroreflectors characterized by a reflected beam divergence of roughly twice the emitted laser beam divergence of 15 µrad. This provides water vapour profiles with a vertical sampling of 110 m; preliminary calculations suggest that the system could detect concentrations of less than 5 ppm. A secondary payload of a fairly conventional medium resolution multispectral radiometer allows wide-swath cloud and aerosol imaging. The total weight and power of the system are estimated at 3 tons and 2,700 W respectively. This novel concept presents significant challenges, including the performance of the lasers in space, the tracking between the main spacecraft and the retroreflectors, the refractive effects of turbulence, and the design of the telescopes to achieve a high signal-to-noise ratio for the high precision measurements. The mission concept was conceived at the Alpbach Summer School 2010.

We have discovered an Aleuria Aurantia Lectin (AAL)-reactive immunoglobulin G (IgG) that naturally occurs in the circulation of rabbits and mice, following immune responses induced by various foreign antigens. AAL can specifically bind to fucose moieties on glycoproteins. However, most serum IgGs are poorly bound by AAL unless they are denatured or treated with glycosidase. In this study, using an immunogen-independent AAL-antibody microarray assay that we developed, we detected AAL-reactive IgG in the sera of all animals that had been immunized 1-2 weeks previously with various immunogens with and without adjuvants and developed immunogen-specific responses. All of these animals subsequently developed immunogen-specific immune responses. The kinetics of the production of AAL-reactive IgG in mice and rabbits were distinct from those of the immunogen-specific IgGs elicited in the same animals: they rose and fell within one to two weeks, and peaked between four to seven days after exposure, while immunogen-specific IgGs continued to rise during the same period. Mass spectrometric profiling of the Fc glycoforms of purified AAL-reactive IgGs indicates that these are mainly comprised of IgGs with core-fucosylated and either mono- or non-galactosylated Fc N-glycan structures. Our results suggest that AAL-reactive IgG could be a previously unrecognized IgG subset that is selectively produced at the onset of a humoral response.

Changes in glycosylation have long been associated with disease. While there are many methods to detect changes in glycosylation, plant derived lectins are often used to determine changes on specific proteins or molecules of interest. One change in glycosylation that has been observed by us and by others is a disease or antigen associated increase in fucosylation on N-linked glycans. To measure this change, the fucose binding Aleuria aurantia lectin (AAL) is often utilized in plate and solution based assays. AAL is a mushroom derived lectin that contains five fucose binding sites that preferentially bind fucose linked (α-1,3, α-1,2, α-,4, and α-1,6) to N-acetyllactosamine related structures. Recently, several reports by us and by others have indicated that specific fucose linkages found on certain serum biomarker glycoprotein's are more associated with disease than others. Taking a site-directed mutagenesis approach, we have created a set of recombinant AAL proteins that display altered binding affinities to different analytes containing various fucose linkages.

Nanosatellite constellations at low Earth orbits represent a cost-efficient way for the accomplishment of Earth and space observation tasks. With the growing complexity of the exploration tasks, the amount of science data is expected to increase. For data download purposes efficient communication strategies must be adopted. The available power of a single nanosatellite spacecraft is limited by the small area of solar arrays. The communications equipment consumes a substantial amount of power. A constellation of nanosatellites allows to share resources efficiently, assigning major tasks to different spacecrafts. Within the constellation, a dedicated spacecraft which acts as data relay, monitoring and controlling inter-satellite communications is advantageous. This allows all other satellites to reuse the energy saved by implementing low-energy consuming communications links between the satellites. With this approach, taking advantage of scheduled communication contacts, the overall system performance can be maximized. For the envisaged data relay scenario, the adoption of advanced communication protocol concepts is required. This paper presents innovative solutions for the fulfilment of the described communications tasks. After an investigation of the system requirements, strategies for the interconnection of the spacecrafts for the creation of a small constellation network are provided. Suitable transport protocol implementations are investigated and efficient store-and-forward concepts are proposed. A communication architecture for the usage of a data relay satellite within the constellation is presented, describing the role of the different nodes in the network.

A major effort in current developments for future space missions is the improvement of communications capabilities. With increasing interest in space research and long-term exploration programs including a variety of both near Earth and deep space missions of the major space agencies, communications faces additional challenges. Next-generation communication systems must be able to cope with long-distance communication links, supporting disruption-tolerant networking (DTN), implementing efficient store-and-forward strategies for data download, and providing interoperability between missions. Therefore, innovative concepts for the creation of an Interplanetary Internet (IPN) are currently under investigation. The Interplanetary Internet Special Interest Group (IPNSIG) has already elaborated a suitable IPN network architecture and identified key research areas.Since the main challenges for IPN are efficient data transport strategies, current research activities are focused on the transport layer. However, in order to achieve these goals, improvements at all OSI layers are necessary. The presented paper deals with future developments required for the establishment of an Interplanetary Internet. After introducing an IPN network architecture proposed by [1], the suitability of terrestrial Internet and existing space communications standards for IPN is analyzed. Current protocol developments for IPN are introduced and compared with the previously investigated standards. Finally, future IPN developments are presented. This analysis focuses on all OSI layers, providing a set of recommendations for the improvement of existing technologies and proposing some innovative concepts and solutions.

Since space missions have been flown, a proper data return to Earth was inherently required. The complexity level and the number of space experiments have increased over the last decades. As a consequence, higher data rates have been required. The most limiting factor on achievable data rates for interplanetary communications is the distance. It imposes challenges on all parts of the communication system. Communication user and system requirements must be carefully defined to fulfill the mission needs. These requirements are expected to increase in the future. Hence, we studied 13 generic future ESA space mission scenarios comprising 5 Lunar missions, 5 Mars missions, and three missions to special targets (Lagrange point L2, NEO, and the Jovian satellite Europa) based on planned and already flown missions in order to derive communication user and system requirements. These requirements were classified, scaled, adapted and validated by calculations for these missions defined in. Within this paper we will present how communication user and system requirements for future space science missions can be classified, scaled and applied. Therefore, after introducing a proper classification of communication requirements we will present recommendations by means of concrete examples assuming an Asteroid Sample Return mission scenario. Some practical hints will be provided as well.

Originally identified as the cellular targets of immunosuppressant drugs, the immunophilins encompass two ubiquitous protein families: the FK-506 binding proteins or FKBPs, and the cyclosporin-binding proteins or cyclophilins. Present in organisms ranging from bacteria to animals and plants, these proteins are characterized by their enzymatic activity; the peptidyl-prolyl cis-trans isomerization of polypeptides. Whilst this function is important for protein folding, it has formed the functional basis for more complex interactions between immunophilins and their target proteins. Beginning with a brief historical overview of the immunophilin family, and a representative illustration of the current state of knowledge that has accumulated for these proteins in diverse organisms, a detailed description is presented of the recent advances in the elucidation of the role of this ubiquitous protein family in plant biology. Though still in its infancy, investigation into the function of plant immunophilins has so far yielded interesting results-as a significant component of the chloroplast proteome, the abundance of immunophilins located in the thylakoid lumen suggests that these proteins may play important roles in this relatively uncharacterized subcellular compartment. Moreover, the importance of the complex multidomain immunophilins in functions pertaining to development is underscored by the strong phenotypes displayed by their corresponding mutants.

The discovery of the family of highly effective and specific fungal metabolites cyclosporin, FK-506, and rapamycin ranks among one of the most important advancements in the field of organ transplantation: these compounds have been shown to act as potent immunosuppressants and their administration to

Change in redox status has long been known to link light to the posttranslational regulation of chloroplast enzymes. So far, studies have been conducted primarily with thioredoxin-linked members of the stroma that function in a broad array of biosynthetic and degradatory processes. Consequently, little is known about the role of redox in regulating the growing number of enzymes found to occur in the lumen, the site of oxygen evolution in thylakoid membranes. To help fill this gap, we have studied AtFKBP13, an FKBP-type immunophilin earlier shown to interact with a redox-active protein of the lumen, and found the enzyme to contain a pair of disulfide bonds in x-ray structural studies. These disulfides, which in protein mutagenesis experiments were shown to be essential for the associated peptidyl-prolyl isomerase activity, are unique to chloroplast FKBPs and are absent in animal and yeast counterparts. Both disulfide bonds were redox-active and were reduced by thioredoxin from either chloroplast or bacterial sources in a reaction that led to loss of enzyme activity. The results suggest a previously unrecognized paradigm for redox regulation in chloroplasts in which activation by light is achieved in concert with oxygen evolution by the oxidation of sulfhydryl groups (conversion of SH to S-S). Such a mechanism, occurring in the thylakoid lumen, is in direct contrast to regulation of enzymes in the stroma, where reduction of disulfides targeted by thioredoxin (S-S converted to SH) leads to an increase in activity in the light.

Selon l'invention, des taux eleves de GP73 dans les serums permettent de diagnostiquer le carcinome hepatocellulaire. Une augmentation des taux de GP73 serique au fil du temps indique egalement l'apparition d'un carcinome hepatocellulaire chez des sujets consideres a risque.