Within the context of nursing education in Australia, the registered nurse (RN) preceptor plays an invaluable role with nursing students; however, many are not specifically trained for this role. This study explored the perceptions of practicing preceptors from one health care facility after completion of a specially designed preceptor program. Results indicated that the participants perceived that the program had increased their knowledge of teaching and learning and increased their skills as preceptors. Further results suggested that when the RN preceptor’s knowledge of the teaching and learning process increased, it led to an increase in the RN preceptor’s generic preceptor skills. This increased the RN preceptor’s confidence, leading to a more positive attitude toward student nurses and a positive effect on preceptor functioning, enhancing the preceptor’s ability to include students more in daily nursing tasks.