目的:利用生物信息学方法识别口腔扁平苔藓(OLP)发病过程中的生物学通路及关键基因或分子.方法:从GEO数据库中下载GSE38616,通过t检验结合Benjamini-Hochberg校正的方法筛选差异表达基因,并根据差异表达基因构建蛋白质互作网络,利用Cytoscape进行网络中心(Hub)节点识别,最后对差异表达基因进行富集分析.对Hub节点基因在20例OLP患者和20例健康对照者外周血中的表达进行检测.结果:在OLP与健康对照组之间共有212个差异表达基因.在构建的蛋白质网络中,所识别的Hub节点为GNB2L1,其在OLP患者外周血的表达显著高于健康对照组(P ＜0.001).富集分析结果显示,212差异表达基因富集的通路有29个.所富集的细胞生物学过程有:信号转导、免疫反应、细胞通讯,类固醇代谢、突触小泡转运等.结论:本研究所识别出来的29条通路及Hub节点GNB2L1可能在OLP发生发展中起到关键作用.

Objective:To research the expression of matrix metalloprotase 9 (MMP-9) in the saliva and the relationship with histopathologic grading of patients with Sjogren Syndrome.Methods:The expressions of MMP-9 in the saliva of 27 Sjogren Syndrome patients (experimental group) and 29 underlip mucocele patients (control group) were detected with ELISA and the results of Sjogren Syndrome patients were pathological graded with low level (1 and 2) and high level (3 and 4).Results:In contrast to control group,the MMP-9 levels in Sjogren Syndrome patients were significantly increased (P＜0.05),and the level of MMP-9 in the high pathologic grade was higher than that of low grade (P＜0.05).Conclusion:The level of MMP-9 in the saliva may be a test indicator for the Sjogren Syndrome,which might provide reference for clinical diagnosis and treatment.

目的：通过观察二氧化锆全瓷冠与烤瓷熔附金属全冠修复前牙缺损后，牙周组织的变化，进行两者用于前牙缺损修复的对比研究。方法选取2015年9月-2016年9月在哈尔滨医科大学附属第二医院口腔修复科进行前牙修复的就诊患者60例。经患者自愿同意将所选择修复方式分为两组：二氧化锆全瓷冠修复者(观察组)，烤瓷熔附金属全冠修复者(对照组)。结果观察组的牙龈缘色素沉着发生率、牙龈乳头颜色的变化、牙龈炎的发生率都明显低于对照组。结论应用二氧化锆全瓷冠修复美观度较高，因此，临床上可作为前牙的美容修复。

口腔修复学的牙列缺失章节是口腔医学生的重要、抽象的专业课程,学生进入临床实习阶段很难将理论知识与实践结合,把PBL教育模式适当地运用于牙列缺失的实习教学中,可以激发学生学习兴趣,提高其提出问题、分析问题的能力,增强学习的积极性、主动性,有利于培养学生的综合素质.本文探讨了运用PBL教学法进行临床教学对学生、病人和教师所带来的益处,为口腔牙列缺失教学以至口腔医学教学方法的改革提供一些经验.

Objective To explore the application and effect of case-based teaching methods used in teaching of stomatology.Methods Compared to the degee of satisfaction and the specialized knowledge written examination between the case-based teaching methods and the traditional teaching methods.Results The test scores and satisfaction survey of the case-based teaching methods were higher than the traditional teaching methods.The experimental results were statistically significant difference(P<0.05).Conclusion The case-based teaching methods can improve the students' interest in learning and academic achievement.

目的:观察腓骨修复重建下颌骨缺损的临床疗效.方法:对采用游离腓骨瓣治疗下颌骨缺损18例进行疗效观察并随访,调查患者对术后面型满意度及义齿修复后咀嚼效果.结果:18例移植腓骨瓣全部成活.全部病例均获随访,所有患者对面型满意(100%),绝大部分病例术后义齿修复(83%),咀嚼功能恢复好.结论:游离腓骨瓣不仅可以提供充足的移植骨量及软组织量,还能保证充足的血液供应,修复重建下颌骨缺损临床疗效良好.

目的:观察针刺治疗纤维肌痛综合征的疗效.方法:治疗组28例采用辨证归经针刺的方法治疗,并设28例为对照组采用口服药物治疗,疗效判定采用国际公认可信度高的MPQ问卷,1疗程后两组进行治疗前后对比.结果:治疗组总有效率96.4%,对照组总有效率64.3%,两组经统计学分析,P＜0.001.结论:辨证归经针刺方法优于常规药物治疗.