The upland cotton (Gossypium hirsutum L.) germplasm lines PX06520‐42‐2‐1 (Reg. No. GP‐1005, PI 677343) and PX06520‐42‐2‐3 (Reg. No. GP‐1006, PI 677344) were jointly released by the USDA‐ARS and Cotton Incorporated in 2016. The lines were released to provide broadly adapted, high fiber quality resources for facilitating fiber improvement efforts across the US Cotton Belt. PX06520‐42‐2‐1 and PX06520‐42‐2‐3 originated from the cross SG747/NM49//AGC85/4364‐43, followed by individual plant selection in the F2, F3, and F4 generations. The two best progeny lines, determined from replicated tests conducted at College Station, TX, and Florence, SC, in 2011 and 2012, were entered into the Regional Breeders Testing Network tests across the US upland Cotton Belt in 2013 and 2014. In 2013, both PX06520‐42‐2‐1 and PX06520‐42‐2‐3 were superior to the check cultivars ‘DP 393’, ‘FM 958’, and ‘SG 105’ for fiber length, fiber length uniformity, fiber strength, and short fiber content. In 2014, PX06520‐42‐2‐1 produced a fiber length, fiber length uniformity, and fiber strength that were superior to the five check cultivars DP 393, FM 958, SG 105, ‘UA 222’, and ‘DP 491’. Fiber yield of PX06520‐42‐2‐1 did not differ significantly from any of the five check cultivars used in 2014. The two lines are excellent sources of fiber quality, with acceptable yield potentials that appear to be stable across production environments.

Information on gene expression during cotton fiber development for high fiber quality genotypes including Pima (Gossypium barbadense L.) and Acala cotton (G. hirsutum L.) is lacking, impeding the discovery of novel genes and DNA markers for fiber quality traits. In the present study, massively parallel sequencing technology was used to generate a total of 394,301 expressed sequence tag (EST) sequence reads, totaling over 42 million bp, from immature ovaries at 10 days post-anthesis of Pima Phy 76 and Acala 1517-99. For Pima Phy 76, 1,900 EST contigs were tentatively assembled from 21.8% (50,876) ESTs which resulted in 1,541 tentative consensus sequences (TCs). 1,005 and 1,064 EST contigs were homologous to TCs from the A2 and D5 genomes, respectively, while 738 of the EST contigs were shared by both genomes. For Acala 1517-99, 2,151 EST contigs were tentatively assembled from 24.5% (39,368) ESTs and resulted in 1,787 TCs. 1,220 and 1,206 EST contigs were homologous to known transcripts from A2 and D5 genomes and only 452 of the EST contigs were shared by both genomes. 691 EST contigs are also homologous (E-value ≤ 1 × 10−10) between Pima Phy 76 and Acala 1517-99, of which 38 contigs pairs were highly homologous (E-value ≤ 1 × 10−60) with sequence variations, predominantly single nucleotide polymorphisms (SNPs). A Blast search of a sample of contigs to Upland cotton ESTs in GenBank indicated that more than 75% EST contigs may contain SNPs. This report represents the first research in cotton using 454 pyrosequencing technology to enrich ESTs for high fiber quality cotton.

Numerous quantitative trait loci (QTLs) have been identified from segregating hybrid populations between Upland (Gossypium hirsutum) and Pima (G. barbadense) cotton. However, the genetic basis of these QTLs is currently unknown. In the present study, an interspecific backcross inbred line (BIL) population was tested at two locations in 2005 and 2006, and used to identify and isolate polymorphic transcript-derived fragments (TDFs) based on complementary DNA (cDNA)-amplified fragment length polymorphism (AFLP) analysis. The BILs were developed from a cross between Upland SG 747 as the recurrent parent and Pima S-7, followed by two generations of backcrossing and three generations of selfing. Of a total of 675 cDNA-AFLP fragments amplified from 15 primer combinations, 143 loci showed qualitative differences (TDFs) among BILs, and 44 TDF loci were significantly correlated with fiber yield and quality traits. Of the more than half of the TDFs cloned and sequenced, 47.4% had unknown function and many were converted to sequence tagged site (STS) markers. The identification of indels was informative in resolving homologous sequences when comparing cDNA-AFLP libraries from the same primer combination among Upland SG 747, Acala 1517-99, and Pima Phy 76. Differential expression of selected subset of TDFs associated with fiber traits was detected by reverse transcription-polymerase chain reaction (RT-PCR) using ovaries of eight selected BILs at 10 days post anthesis (DPA), and SG 747 and Pima 76 at 0, 4, 8, and 13 days. Quantitative RT-PCR was further used for validation on five TDFs using ovaries of Acala 1517-99 and Pima Phy 76 from the four early boll developmental stages. The present study has identified a number of genes whose expression was associated with fiber yield and quality traits.

The upland cotton (Gossypium hirsutum L.) germplasm line CRB 252 (Reg. No GP‐925, PI 658596) was developed, evaluated, and jointly released in 2009 by the USDA‐ARS, the Louisiana Agricultural Experiment Station, the Georgia Agricultural Experiment Station, and Cotton Incorporated. The purpose of the release was to provide a broadly adapted, high fiber‐quality resource for facilitating fiber improvement efforts across the U.S. Cotton Belt. CRB 252 originated from the cross ‘Suregrow 248’/‘Phytogen 72’//‘Stoneville 474’/‘Acala Maxxa’, followed by individual plant selection in the F2 and F3 generations at the low‐desert location of Maricopa, AZ and F3.5 progeny selection at five locations distributed across the Cotton Belt. Evaluation of CRB 252 occurred in 13 location‐year environments at Florence, SC; Blackville, SC; Tifton, GA; Plains, GA; Alexandria, LA; Maricopa, AZ; and Shafter, CA in 2007 and 2008. CRB 252 displayed fiber length and micronaire values superior to those of the high fiber‐quality check cultivars Phytogen 72 and FM 958. The fiber strength and short‐fiber content of CRB 252 was superior to those of FM 958. Lint yield of CRB 252 was superior to that of Phytogen 72 and did not differ from FM 958. CRB 252 is an excellent source of quality fiber, with acceptable yield potential that appears to be stable across production environments.

Non-coding sequences account for a majority of the higher plant genome, some of which have important effects in gene regulation and plant development. In an effort to develop molecular marker systems to search for polymorphisms associated with high fiber yield and quality in cotton, we have developed a methodology that could specifically target the regulatory regions of the cotton genome. In this study we designed 10-nucleotide degenerate promoter primers based on conserved core promoter sequences and tested their applicability in PCR amplifications in combination with 10-mer random amplified polymorphic DNA (RAPD) primers. The amplified markers are called promoter anchored amplified polymorphism based on RAPD (PAAP-RAPD). Forty cotton genotypes with diverse genetic and geographical backgrounds were used to test the PAAP-RAPD system using polyacrylamide gel electrophoresis. Based on PAAP-RAPD markers amplified from 12 primer combinations, the 40 genotypes were classified into five distinctive groups: two Upland cotton (Gossypium hirsutum) groups from China, another two Upland cotton groups from the USA, and one group from American Pima cotton (G. barbadense). The groupings are in general consistent with their genetic and geographical origins. Thirty-six PAAP-RAPD and RAPD fragments were cloned and four of them were further subjected to sequence analysis. Signal scanning using software PLACE confirmed that they contained an array of cis-regulatory sequences in addition to the core promoter sequences. The results demonstrate the potential application of PAAP-RAPD as a new marker system specifically targeting regulatory regions of the plant genome.

The lack of genetic diversity within cultivated upland cotton (Gossypium hirsutum L.) has hindered the construction of genomewide linkage maps and their applications in genetics and breeding. The objective of this investigation was to develop candidate gene markers for fiber quality and yield on the basis of approximately 90 genes implicated in fiber development. Polymorphisms using sequence-tagged site (STS) and single nucleotide polymorphism (SNP) markers based on single strand conformation polymorphism (SSCP) and cleaved amplified polymorphism (CAP) were evaluated among three upland and five Pima cotton (G. barbadense L.) genotypes. Of the 90 primer pairs, 75 resulted in polymerase chain reaction amplifications, including 11 that yielded polymorphic STS markers. Of the 48 primer pairs that produced polymorphic SSCP markers, 27 yielded interspecific polymorphism, while 15 yielded both inter- and intraspecific polymorphisms. Six pairs yielded only intraspecific polymorphisms. A total of 18 SNPs, including four indels, were identified in seven of the 15 fiber gene fragments on the basis of direct DNA sequencing, and the average length was 350 bp, with a mean of 1.3 SNPs per fragment. The average rate of SNPs per nucleotide was 0.34%, and 0.31% and 0.41% in coding and noncoding regions, respectively. Eight of the 15 SNPs were interspecific and 78% were nucleotide substitutions, with the four indels contributing to interspecific polymorphism. Six selected SNPs were confirmed by restriction enzyme digestion. The high level of SSCP polymorphism observed within a selected set of agronomically improved lines of upland cotton suggests that the use of SSCP will greatly facilitate genomewide mapping in upland cotton.

Crop ScienceVolume 46, Issue 4 p. 1828-1829 Registrations of Germplasm Registration of AGC85, AGC208, and AGC375 Upland Cotton Germplasm Lines R.G. Percy, Corresponding Author R.G. Percy [email protected] USDA-ARS, U.S. Arid Land Agricultural Research Center, 21881 N. Cardon Lane, Maricopa, AZ, 85239Corresponding author ([email protected])Search for more papers by this authorO.L. Mayo, O.L. Mayo Dep. of Crop & Soil Sci., Univ. Georgia, Coastal Plain Station, P.O. Box 748, Tifton, GA, 31793Search for more papers by this authorM. Ulloa, M. Ulloa USDA-ARS, 17053 N. Shafter Ave., Shafter, CA, 93263Search for more papers by this authorR.G. Cantrell, R.G. Cantrell Cotton Incorporated, 6399 Weston Pkwy., Cary, NC, 27513Search for more papers by this author R.G. Percy, Corresponding Author R.G. Percy [email protected] USDA-ARS, U.S. Arid Land Agricultural Research Center, 21881 N. Cardon Lane, Maricopa, AZ, 85239Corresponding author ([email protected])Search for more papers by this authorO.L. Mayo, O.L. Mayo Dep. of Crop & Soil Sci., Univ. Georgia, Coastal Plain Station, P.O. Box 748, Tifton, GA, 31793Search for more papers by this authorM. Ulloa, M. Ulloa USDA-ARS, 17053 N. Shafter Ave., Shafter, CA, 93263Search for more papers by this authorR.G. Cantrell, R.G. Cantrell Cotton Incorporated, 6399 Weston Pkwy., Cary, NC, 27513Search for more papers by this author First published: 01 July 2006 https://doi.org/10.2135/cropsci2005.12-0483Citations: 6 Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article.Citing Literature Volume46, Issue4July–August 2006Pages 1828-1829 RelatedInformation

Crop ScienceVolume 46, Issue 5 p. 2336-2338 Registrations of Germplasms Registration of SJ-U86 Cotton Germplasm Line with High Yield and Excellent Fiber Quality M. Ulloa, Corresponding Author M. Ulloa mulloa@pw.ars.usda.gov USDA-ARS, WICS Research Unit, 17053 N. Shafter Ave., Shafter, CA, 93263Corresponding author (mulloa@pw.ars.usda.gov)Search for more papers by this authorR.G. Percy, R.G. Percy USDA-ARS, 37860 W. Smith-Enke Road, Maricopa, AZ, 85239Search for more papers by this authorR. Hutmacher, R. Hutmacher Univ. of California-Shafter, 17053 N. Shafter Ave., Shafter, CA, 93263Search for more papers by this authorR.G. Cantrell, R.G. Cantrell Cotton Incorporated, 6399 Weston Pkwy., Cary, NC, 27513Search for more papers by this author M. Ulloa, Corresponding Author M. Ulloa mulloa@pw.ars.usda.gov USDA-ARS, WICS Research Unit, 17053 N. Shafter Ave., Shafter, CA, 93263Corresponding author (mulloa@pw.ars.usda.gov)Search for more papers by this authorR.G. Percy, R.G. Percy USDA-ARS, 37860 W. Smith-Enke Road, Maricopa, AZ, 85239Search for more papers by this authorR. Hutmacher, R. Hutmacher Univ. of California-Shafter, 17053 N. Shafter Ave., Shafter, CA, 93263Search for more papers by this authorR.G. Cantrell, R.G. Cantrell Cotton Incorporated, 6399 Weston Pkwy., Cary, NC, 27513Search for more papers by this author First published: 01 September 2006 https://doi.org/10.2135/cropsci2006.04.0235Citations: 1 Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume46, Issue5September–October 2006Pages 2336-2338 RelatedInformation

ABSTRACT Genetic variation available for the improvement of fiber properties is restricted in commercial upland cotton ( Gossypium hirsutum L.). Resources for fiber improvement exist in G. barbadense L., but introgression of traits has been a limited success. The objectives of this study were to investigate the genetic variation and heritability of agronomic and fiber traits within a diverse recombinant inbred line (RIL) population created with a stable introgressed parent. The population ( n = 98 lines) had as its parents NM24016, a stable G. hirsutum line with significant introgression from G. barbadense , and TM1, the G. hirsutum genetic standard. Yield, plant height, boll size, lint percentage, and fiber length, strength, micronaire, and elongation were measured in randomized, complete block tests at Las Cruces, NM, and Maricopa, AZ, in 2001 and 2002. Genotype coefficients of variation (CV) were highest for plant height and boll size. Among fiber traits, fiber length and micronaire produced the highest genotype CVs. Most traits (fiber elongation excepted) exhibited high broadsense heritability, ranging from 0.69 for lint yield to 0.92 for 2.5% span length. Transgressive segregants were identified for most traits. Fiber strength and 2.5% span length were favorably correlated ( r = 0.59, P = 0.001) as were 2.5% span length and micronaire ( r = −0.47, P = 0.001). The NM24016/TM1 RIL population presents valuable genetic variation for fiber quality improvement efforts in G. hirsutum .

Interspecific chromosome substitution is among the most powerful means of introgression and steps toward quantitative trait locus (QTL) identification. By reducing the genetic “noise” from other chromosomes, it greatly empowers the detection of genetic effects by specific chromosomes on quantitative traits. Here, we report on such results for 14 cotton lines (CS-B) with specific chromosomes or chromosome arms from G. barbadense L. substituted into G. hirsutum and chromosome-specific F2 families. Boll size, lint percentage, micronaire, 2.5% span length, elongation, strength, and yield were measured by replicated field experiments in five diverse environments and analyzed under an additive–dominance (AD) genetic model with genotype and environment interaction. Additive effects were significant for all traits and dominance effects were significant for all traits except 2.5% span length. CS-B25 had additive effects increasing fiber strength and fiber length and decreasing micronaire. CS-B16 and CS-B18 had additive effects related to reduced yields. The results point toward specific chromosomes of G. barbadense 3-79 as the probable locations of the genes that significantly affect quantitative traits of importance. Our results provided a scope to analyze individual chromosomes of the genome in homozygous and heterozygous conditions and thus detected novel effects of alleles controlling important QTL.

Crop ScienceVolume 42, Issue 3 p. 988-988 Registration of Germplasm Registration of Five Extra-Long Staple Cotton Germplasm Lines Possessing Superior Fiber Length and Strength R.G. Percy, Corresponding Author R.G. Percy rpercy@ag.arizona.edu USDA-ARS, Maricopa Agricultural Center, 37860 W. Smith-Enke Road, Maricopa, AZ, 85239Corresponding author (rpercy@ag.arizona.edu)Search for more papers by this author R.G. Percy, Corresponding Author R.G. Percy rpercy@ag.arizona.edu USDA-ARS, Maricopa Agricultural Center, 37860 W. Smith-Enke Road, Maricopa, AZ, 85239Corresponding author (rpercy@ag.arizona.edu)Search for more papers by this author First published: 01 May 2002 https://doi.org/10.2135/cropsci2002.9880Citations: 4 Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume42, Issue3May–June 2002Pages 988-988 RelatedInformation

Crop ScienceVolume 41, Issue 2 p. 602-603 Registration of Germplasm Registration of PS-6ne, PS-6L°, PS-6neL°, P62ne, P62L°, and P62neL° Extra-long Staple Cotton Germplasm R.G. Percy, Corresponding Author R.G. Percy rpercy@ag.arizona.edu USDA-ARS, Maricopa Agricultural Center, 37860 W. Smith-Enke Rd., Maricopa, AZ, 85239Corresponding author (rpercy@ag.arizona.edu)Search for more papers by this author R.G. Percy, Corresponding Author R.G. Percy rpercy@ag.arizona.edu USDA-ARS, Maricopa Agricultural Center, 37860 W. Smith-Enke Rd., Maricopa, AZ, 85239Corresponding author (rpercy@ag.arizona.edu)Search for more papers by this author First published: 01 March 2001 https://doi.org/10.2135/cropsci2001.412602-axCitations: 3 Registration by CSSA. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Citing Literature Volume41, Issue2March–April 2001Pages 602-603 RelatedInformation

Morphological mutants of cotton (Gossypium spp.) have been used extensively in genetic mapping studies and in several instances have proven useful in agronomic improvement efforts. This investigation was conducted to determine the inheritance, allelism, and linkage associations of two spontaneous mutants found in plants of American Pima (G. barbadense L.) cotton. The two mutants were crossed to the normal phenotype cultivar PS‐6 and to several genetic marker stocks. Appropriate F1, F2, and BC populations were created for analyses of inheritance and linkage studies. Analyses of F1 and F2 populations of a virescent mutant indicated that it is inherited as an extranuclear factor. Expression of the mutant is transient, being strongest in the first true leaf after germination and fading by the fourth or fifth true leaf. It is proposed that the mutant be designated cytoplasmicvirescent and be assigned the gene symbol cyt‐V. Analyses of F1, F2, and BC populations of a second, juvenilely expressed, densely glanded mutant indicated that it is inherited as a single, recessively expressed gene. Tests for association of the dense glanding trait with 13 other mutant markers produced no evidence of linkage. Linkage tests with two markers, T1 and ms13, were inconclusive. Expression of the dense glanding mutant is confined to expanding leaves and internodes, and to bracteoles. Expression is strongest in the first and second apical leaves, and fades by the fourth or fifth leaf. The designation denseglanding and gene symbol dg are assigned to the mutant.

Recent studies of historical series from Pima cotton and bread wheat bred for higher yields at supra-optimal temperatures under ample water supply have shown that incremental increases in stomatal conductance have accompanied lint and grain yield increases in successive commercial releases. Pima cotton studies showed that the differences in stomatal conductance are under genetic control. F 4 progeny of F 2 plants selected solely for high stomatal conductance had higher lint yields than progeny from low conductance plants. Carbon isotope discrimination is positively correlated with stomatal conductance and yields in both wheat and Pima cotton. A gas exchange study showed that the stomatal response to temperature, but not to light or to water vapour pressure deficit (VPD), separated low and high-yielding Pima lines in the same order as their stomatal conductance in field conditions. Selection for higher yields in Pima cotton and bread wheat appear to have generated selection pressures for higher stomatal conductances that are independent of operating pressures for higher photosynthetic rates. The adaptive advantage of higher stomatal conductance appears to be associated with leaf cooling, which provides an avoidance type of heat resistance at supra-optimal temperatures. Lower leaf and canopy temperatures at critical developmental stages associated with flowering and fruiting during July for Pima cotton in Arizona, and February for bread wheat in north-west Mexico appear to favour higher yields. Stomatal conductance could be a valuable selection criterion for higher yields in irrigated crops grown at supra-optimal temperatures.

Crop ScienceVolume 38, Issue 5 cropsci1998.0011183X003800050062x p. 1409-1409 Registration of GermplasmFull Access Registration of Extra-Long Staple Cotton Germplasm, 89590 and 8810 R. G. Percy, R. G. PercySearch for more papers by this authorE. L. Turcotte, E. L. TurcotteSearch for more papers by this author R. G. Percy, R. G. PercySearch for more papers by this authorE. L. Turcotte, E. L. TurcotteSearch for more papers by this author First published: 01 September 1998 https://doi.org/10.2135/cropsci1998.0011183X003800050062xCitations: 15AboutRelatedInformationPDFPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessClose modalShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article.Citing Literature Volume38, Issue5September–October 1998Pages 1409-1409 RelatedInformation RecommendedRegistration of Eight Extra‐Long Staple Upland Cotton Germplasm LinesC. W. Smith, S. Hague, P. S. Thaxton, E. Hequet, D. Jones, Journal of Plant RegistrationsExtra Long Staple Upland Cotton for the Production of Superior YarnsKolbyn Joy, C. Wayne Smith, Eric Hequet, S. Ed Hughs, Steve Hague, Crop ScienceRegistration of three Arkot 0908 cotton germplasm linesFred M. Bourland, Peng Chee, Don C. Jones, Journal of Plant RegistrationsDevelopment of Extra‐Long Staple Upland CottonC. Wayne Smith, S. Hague, E. Hequet, P. S. Thaxton, I. N. Brown, Crop ScienceRegistration of Arkot 1005, Arkot 1015, and Arkot 1019 cotton germplasm linesFred Bourland, Don Jones, Journal of Plant Registrations

Crop ScienceVolume 37, Issue 2 cropsci1997.0011183X003700020061x p. 632-633 Registration of Germplasm Registration of 10 Pima Cotton Germplasm Lines, P70 to P79 R. G. Percy, Corresponding Author R. G. Percy rpercy@ag.arizona.edu USDA-ARS, Maricopa Agricultural Center, 37860 W. Smith-Enke Rd., Maricopa, AZ, 85239Corresponding author (rpercy@ag.arizona.edu).Search for more papers by this authorE. L. Turcotte, E. L. Turcotte USDA-ARS, Maricopa Agricultural Center, 37860 W. Smith-Enke Rd., Maricopa, AZ, 85239Search for more papers by this author R. G. Percy, Corresponding Author R. G. Percy rpercy@ag.arizona.edu USDA-ARS, Maricopa Agricultural Center, 37860 W. Smith-Enke Rd., Maricopa, AZ, 85239Corresponding author (rpercy@ag.arizona.edu).Search for more papers by this authorE. L. Turcotte, E. L. Turcotte USDA-ARS, Maricopa Agricultural Center, 37860 W. Smith-Enke Rd., Maricopa, AZ, 85239Search for more papers by this author First published: 01 March 1997 https://doi.org/10.2135/cropsci1997.0011183X003700020061xCitations: 6AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat No abstract is available for this article.Citing Literature Volume37, Issue2March–April 1997Pages 632-633 RelatedInformation

Gossypol, a triterpenoid aldehyde found in all species of the genus Gossypium, possesses insecticidal, antimicrobial, antifertility, and antitumor properties. Gossypol content in cotton seed varies among cultivated species and among cultivars within species. This investigation was conducted at the Texas Agricultural Research Center in San Angelo, TX, in 1993 to determine the regional and geographic variability for seed gossypol content and isomer form in G. barbadense L. A set of 57 accessions (collected during 50 yr from 21 countries) representing five regions, and 26 cultivars were analyzed. Species′ variation for gossypol and isomer content exceeded previous reports. Total gossypol content in seed ranged from 3.0 to 34.0 g kg‐1 kernel weight. The more biologically active negative isomer ranged from 249 to 689 g kg‐1 total seed gossypol. Cotton accessions collected west of the Andes mountains in South America displayed the greatest variation in seed gossypol and gossypol isomer contents. Accessions from Central Americaa nd Argentina displayed the least variation. Subsets of accessions, possessing unique levels of seed gossypol or gossypol isomer, were identified within regional groups or cultivars. A low (r = 0.02) phenotypic correlation between gossypol content and isomer ratio suggested that simultaneous selection for the two traits is feasible.